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Method for preparing oligo xylose in high purity by using stalk as raw material, and using technique of enzyme and membrane

A high-purity technology of xylooligosaccharides, which is applied in the field of food biochemical industry, can solve the problems of high loss of xylooligosaccharides, achieve simple processes, improve market competitiveness, and accelerate national economic development.

Inactive Publication Date: 2007-07-25
JIANGNAN UNIV
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  • Claims
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AI Technical Summary

Problems solved by technology

Activated carbon is the most commonly used adsorption medium, which has the advantages of strong adsorption, good separation effect, cheap and easy to obtain, but the loss of xylooligosaccharides is relatively large

Method used

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  • Method for preparing oligo xylose in high purity by using stalk as raw material, and using technique of enzyme and membrane
  • Method for preparing oligo xylose in high purity by using stalk as raw material, and using technique of enzyme and membrane

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Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1: enzymatic hydrolysis process.

[0036] Blast the straw, add 5-15 times of water, mix well, adjust the pH to 4-8, the temperature is 40-60°C, add Aspergillus niger xylanase at a ratio of 50-300U / g dry basis, stir for 8-24h, Centrifuge or filter to obtain supernatant. The main evaluation index is the change of total sugar and reducing sugar content.

[0037] Figure 1 and Figure 2 show that the temperature is 53°C, the pH is 6.0, the amount of enzyme added is 132, 198, and 264 U / g dry basis when adding 8 times of water, and the enzyme hydrolysis time is 1, 2, 3, 4, 5, 6, 8, 10, 12, 15 and 18h, the change of total sugar and reducing sugar content. Both total sugar and reducing sugar increased gradually with time, but the rate of increase decreased gradually. The amount of enzyme added has a certain impact on the reaction. The higher the amount of enzyme added, the faster the hydrolysis rate, the higher the proportion of sugar increase in the same enzymatic ...

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Abstract

This invention discloses a method for preparing high-purity xylooligosaccharide from straw by using enzyme and membrane technology. The method comprises: steam-exploding straw, adding xylanase, hydrolyzing, centrifuging or filtering to obtain supernatant, performing nanofiltration or vacuum-concentration, acid-treating, centrifuging or filtering to obtain supernatant, performing active carbon adsorption, ultrafine filtration and ion exchange resin column adsorption, and concentrating or spray-drying to obtain high-purity xylooligosaccharide slurry or powder (xylooligosaccharide content is up to 70-95%). The residues of straw produced during xylooligosaccharide preparation can be used for papermaking, thus is environmentally friendly. Straw has an abundant resource, thus the cost is low. The method has such advantages as high treatment quantity, integrated operation, simple process, and high efficiency.

Description

technical field [0001] A method for preparing high-purity xylooligosaccharides by using enzyme and membrane technology as raw material, involving the preparation process of xylooligosaccharides, specifically involving straw blasting, enzymatic hydrolysis, nanofiltration concentration and separation, and the use of activated carbon, ultrafiltration The invention relates to a method for decoloring and desalting with an ion exchange resin, and belongs to the technical field of food biochemical industry. Background technique [0002] In recent years, oligosaccharides have become popular. Oligosaccharides, also known as oligosaccharides, are a general term for a class of oligosaccharides formed by 2-10 monosaccharides linked by glycosidic bonds to form straight or branched chains, with a molecular weight of about 300-2000. According to its use, it is divided into two categories: functional oligosaccharides and ordinary oligosaccharides. Functional oligosaccharides refer to oligo...

Claims

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Application Information

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IPC IPC(8): C12P19/00
Inventor 杨瑞金蒋琦霞
Owner JIANGNAN UNIV
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