Ganoderma lucidum polysaccharide extracting method

An extraction method and technology of ganoderma polysaccharides, applied in the field of extraction of ganoderma polysaccharides, can solve the problems of high degree of lignification, long extraction time, high extraction temperature, achieve high decolorization rate and polysaccharide yield, reduce the use of enzymes, maintain The effect of biological activity

An extraction method and technology of ganoderma polysaccharides, applied in the field of extraction of ganoderma polysaccharides, can solve the problems of high degree of lignification, long extraction time, high extraction temperature, achieve high decolorization rate and polysaccharide yield, reduce the use of enzymes, maintain The effect of biological activity

CN108341890AInactive Publication Date: 2018-07-31四川岚晟生物科技股份有限公司
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Pulverization: the dried Ganoderma lucidum fruiting body is pulverized by a biomass pulverizer, and passed through an 80-mesh sieve to obtain Ganoderma lucidum powder;

[0034] (2) Steam explosion: put 100g of Ganoderma lucidum powder into the steam explosion device, the explosion pressure is 1.5MPa, the temperature is 150°C, and the explosion is maintained for 2 minutes, and then the pressure in the reactor is reduced to 1 standard atmospheric pressure. After the explosion is completed, we get blasted ganoderma powder;

[0035] (3) Soaking: add the explosive Ganoderma lucidum powder into the reaction tank, add water to soak for 1.5 hours according to the solid-liquid ratio of 1:15, and obtain the material-liquid mixture;

[0036] (4) Ultrasonic treatment: Ultrasonic treatment of the material-liquid mixture for 30 minutes at 40°C, with an ultrasonic power of 4000W;

[0037] (5) Proteolysis: Control the temperature of the feed liquid in the reaction tank to 52°C and...

Embodiment 2

[0044] (1) Pulverization: the dried Ganoderma lucidum fruiting body is pulverized by a biomass pulverizer, and passed through an 80-mesh sieve to obtain Ganoderma lucidum powder;

[0045] (2) Steam explosion: Put 100g of Ganoderma lucidum powder into the steam explosion device, the steam explosion pressure is 1.5MPa, the temperature is 150°C, and the explosion is maintained for 5 minutes, and then the pressure in the reactor is reduced to 1 standard atmospheric pressure. After the explosion is completed, Explosive Ganoderma lucidum powder;

[0046] (3) Soaking: add the explosive Ganoderma lucidum powder into the reaction tank, add water to soak for 1.5 hours according to the solid-to-liquid ratio of 1:10, and obtain the material-liquid mixture;

[0047] (4) Ultrasonic treatment: The material-liquid mixture is ultrasonically treated at 40°C for 30 minutes, and the ultrasonic power is 5000W;

[0048] (5) Proteolysis: Control the temperature of the feed liquid in the reaction ta...

Embodiment 3

[0055](1) Pulverization: the dried Ganoderma lucidum fruiting body is pulverized by a biomass pulverizer, and passed through an 80-mesh sieve to obtain Ganoderma lucidum powder;

[0056] (2) Steam explosion: put 100g of Ganoderma lucidum powder into the steam explosion device, the steam explosion pressure is 2.0MPa, the temperature is 200°C, and the explosion is maintained for 3 minutes, and then the pressure in the reactor is reduced to 1 standard atmospheric pressure. After the explosion is completed, Explosive Ganoderma lucidum powder;

[0057] (3) Soaking: add the explosive Ganoderma lucidum powder into the reaction tank, add water to soak for 1.5 hours according to the solid-to-liquid ratio of 1:20, and obtain the material-liquid mixture;

[0058] (4) Ultrasonic treatment: Ultrasonic treatment of the material-liquid mixture at 50°C for 30 minutes, with an ultrasonic power of 3000W;

[0059] (5) Proteolysis: Control the temperature of the feed liquid in the reaction tank ...

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Abstract

The invention discloses a ganoderma lucidum polysaccharide extracting method. The extracting method comprises the steps: firstly, smashing ganoderma lucidum sporocarp and sieving by a 80-mesh sieve; then putting into a steam explosion device to be exploded, adding into water to be soaked for 1.5h according to a solid to liquid ratio as 1 to (10 to 20) and then heating to 30 to 50 DEG C to performultrasonic treatment; adjusting a material liquid temperature, a pH value, protease and cellulase; after enzymolysis finishes, warming to denaturalize enzyme, centrifuging, decoloring resin, deproteinizing, filtering, vacuum concentrating and vacuum drying under a lower temperature to obtain high-activity and high-purity ganoderma lucidum polysaccharide solid powder. Therefore, a polysaccharide extracting rate and a polysaccharide production efficiency are improved, a space structure of polysaccharide is prevented from being damaged, biological activity of the polysaccharide is prevented frombeing reduced, and the ganoderma lucidum polysaccharide extracting method is more suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to an extraction method of ganoderma lucidum polysaccharide. Background technique [0002] Ganoderma lucidum is a large basidiomycete with high nutritional and health value. Since ancient times, Ganoderma lucidum has been regarded as a symbol of auspiciousness, wealth, beauty and longevity. If the fungus is eaten directly, it generally cannot achieve the therapeutic effect. Usually, its active ingredients can only be extracted, separated and purified to improve the efficacy and exert certain pharmacological effects. A large number of research reports show that: Ganoderma lucidum polysaccharide is the main bioactive component of Ganoderma lucidum, and the various medicinal effects of Ganoderma lucidum are closely related to the activity and content of Ganoderma lucidum polysaccharide, and Ganoderma lucidum polysaccharide has become one of the most impor...

Claims

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Application Information

Patent Timeline
31 Jul 2018
Publication
CN108341890A
IPC
C08B37/00
CPC
C08B37/0003
Inventors
陈彪; 曾淋民