Ganoderma lucidum polysaccharide extracting method

An extraction method and technology of ganoderma polysaccharides, applied in the field of extraction of ganoderma polysaccharides, can solve the problems of high degree of lignification, long extraction time, high extraction temperature, achieve high decolorization rate and polysaccharide yield, reduce the use of enzymes, maintain The effect of biological activity

Inactive Publication Date: 2018-07-31
四川岚晟生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different from other edible fungi, the fruiting body of Ganoderma lucidum has a high degree of lignification and complex structure. Polysaccharides are generally combined with cellulose and lignin through physical or chemical bonds, making the fruiting body of Ganoderma lucidum have strong hardness, and the polysaccharides of Ganoderma lucidum exist in the fruiting body It is difficult to extract polysaccharides from the cell wall of
As can be seen from the current research results, the extraction rate of water-soluble Ganoderma lucidum polysaccharides is only about 3%, and most of the remaining polysaccharides are retained in the residue. If they are directly discarded, resources will be wasted and the environment will be damaged. create pollution
The traditiona

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Example 1

[0033] (1) Crushing: The dried Ganoderma lucidum fruit body is crushed by a biomass pulverizer and passed through an 80-mesh sieve to obtain Ganoderma lucidum powder;

[0034] (2) Steam explosion: Put 100g of Ganoderma lucidum powder into the steam explosion device, the explosion pressure is 1.5MPa, the temperature is 150℃, and the explosion is maintained for 2 minutes. Then the pressure in the reactor is reduced to 1 standard atmosphere. After the explosion is completed, Blasting Ganoderma lucidum powder;

[0035] (3) Soaking: add explosive Ganoderma lucidum powder into the reaction tank, add water to soak for 1.5h according to the solid-liquid ratio of 1:15, to obtain a mixture of material and liquid;

[0036] (4) Ultrasonic treatment: the material-liquid mixture is subjected to ultrasonic treatment at 40°C for 30 minutes, and the ultrasonic power is 4000W;

[0037] (5) Proteolysis: control the temperature of the material in the reaction tank at 52°C and the pH val...

Example Embodiment

[0043] Example 2

[0044] (1) Crushing: The dried Ganoderma lucidum fruit body is crushed by a biomass pulverizer and passed through an 80-mesh sieve to obtain Ganoderma lucidum powder;

[0045] (2) Steam explosion: Put 100g of Ganoderma lucidum powder into the steam explosion device, the steam explosion pressure is 1.5MPa, the temperature is 150℃, and the explosion is maintained for 5 minutes, and then the pressure in the reactor is reduced to 1 standard atmosphere. After the explosion is completed, Get blasting Lingzhi powder;

[0046] (3) Soaking: Put the explosive Ganoderma lucidum powder into the reaction tank, add water to soak for 1.5h according to the solid-to-liquid ratio of 1:10 to obtain a mixture of material and liquid;

[0047] (4) Ultrasonic treatment: the material-liquid mixture is subjected to ultrasonic treatment at 40°C for 30 minutes, and the ultrasonic power is 5000W;

[0048] (5) Proteolysis: control the temperature of the material in the reaction tank at 52°C and ...

Example Embodiment

[0054] Example 3

[0055] (1) Crushing: The dried Ganoderma lucidum fruit body is crushed by a biomass grinder and passed through an 80-mesh sieve to obtain Ganoderma lucidum powder;

[0056] (2) Steam explosion: Put 100g of Ganoderma lucidum powder into the steam explosion device, steam explosion pressure 2.0MPa, temperature 200℃, maintain the explosion for 3 minutes, then reduce the pressure in the reactor to 1 standard atmosphere, after the explosion is completed, Get blasting Lingzhi powder;

[0057] (3) Soaking: add the explosive Ganoderma lucidum powder into the reaction tank, add water to soak for 1.5 hours according to the solid-liquid ratio of 1:20, to obtain a mixture of material and liquid;

[0058] (4) Ultrasonic treatment: the material-liquid mixture is subjected to ultrasonic treatment at 50°C for 30 minutes, and the ultrasonic power is 3000W;

[0059] (5) Proteolysis: control the temperature of the material in the reaction tank at 52°C and the pH value of 7.0, and put th...

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Abstract

The invention discloses a ganoderma lucidum polysaccharide extracting method. The extracting method comprises the steps: firstly, smashing ganoderma lucidum sporocarp and sieving by a 80-mesh sieve; then putting into a steam explosion device to be exploded, adding into water to be soaked for 1.5h according to a solid to liquid ratio as 1 to (10 to 20) and then heating to 30 to 50 DEG C to performultrasonic treatment; adjusting a material liquid temperature, a pH value, protease and cellulase; after enzymolysis finishes, warming to denaturalize enzyme, centrifuging, decoloring resin, deproteinizing, filtering, vacuum concentrating and vacuum drying under a lower temperature to obtain high-activity and high-purity ganoderma lucidum polysaccharide solid powder. Therefore, a polysaccharide extracting rate and a polysaccharide production efficiency are improved, a space structure of polysaccharide is prevented from being damaged, biological activity of the polysaccharide is prevented frombeing reduced, and the ganoderma lucidum polysaccharide extracting method is more suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to an extraction method of ganoderma lucidum polysaccharide. Background technique [0002] Ganoderma lucidum is a large basidiomycete with high nutritional and health value. Since ancient times, Ganoderma lucidum has been regarded as a symbol of auspiciousness, wealth, beauty and longevity. If the fungus is eaten directly, it generally cannot achieve the therapeutic effect. Usually, its active ingredients can only be extracted, separated and purified to improve the efficacy and exert certain pharmacological effects. A large number of research reports show that: Ganoderma lucidum polysaccharide is the main bioactive component of Ganoderma lucidum, and the various medicinal effects of Ganoderma lucidum are closely related to the activity and content of Ganoderma lucidum polysaccharide, and Ganoderma lucidum polysaccharide has become one of the most impor...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 陈彪曾淋民邬佑平陈海强
Owner 四川岚晟生物科技股份有限公司
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