20 results about "Glutamate fermentation" patented technology

The application discloses a preparation method of glutamic acid bacterial protein ecological fertilizer, and the preparation steps are as follows: firstly, zinc polysilicate is added into glutamic acid fermentation waste liquid, and bacterial protein-zinc polysilicate complex is obtained through filtration; then, zinc silicate and hydroxyapatite are added, and amino acid composite hydroxyapatite particles are formed through the action of citric acid; and finally, urea-humic acid-dipotassium hydrogen phosphate complex is sprayed on the amino acid composite hydroxyapatite particles to obtain the ecological fertilizer. The ecological fertilizer can not only fully utilize the bacterial protein in the glutamic acid fermentation waste liquid, but also play a good slow-release effect of nitrogen, phosphorus and other elements through the action of two complexes. In addition, the addition of silicon and zinc elements can play a supplementing effect for the growth of crops, so that the fertilizer has the dual functions of environmental protection and long-acting slow release, and has a good promoting effect on the growth of crops.

The invention relates to the technical field of microbial fermentation, and discloses a fermentation composition with a whitening effect and a preparation method and application thereof. According to the application, Chinese rose, a sodium glutamate culture medium and sprouted mung beans are respectively used as fermentation objects, and are fermented by selecting plant lactobacillus and bacillus subtilis to respectively obtain a Chinese rose fermented product, a glutamic acid fermented product and a mung bean sprout fermented product; compared with the prior art, the arbutin fermented product has the advantages that the arbutin fermented product shows more excellent monophenol enzyme inhibition rate and diphenol enzyme inhibition rate which are superior to those of arbutin, particularly, the effect is optimal when the three fermented products are compounded, and a theoretical basis is provided for development and application of whitening cosmetic raw materials and products.

The invention belongs to the technical field of biology, and discloses a process for improving the fermentation rate of glutamic acid, which comprises the following steps: a fermentation medium containing oligopeptide is used for fermentation, and the amino acid sequence of the oligopeptide contains PSGPR. By optimizing the components of the fermentation culture medium, the density and activity of the strain are improved, so that the glutamic acid fermentation efficiency is promoted to be improved.

The invention belongs to the technical field of separation and extraction of organic matters in high-salt-content wastewater, and discloses a method for extracting organic matters in glutamic acid fermentation tail liquid and determining the content of the organic matters in the glutamic acid fermentation tail liquid and an extracting agent.The high-concentration glutamic acid fermentation tail liquid is filtered firstly and then mixed with the extracting agent.The high-concentration glutamic acid fermentation tail liquid is mixed with the extracting agent.The inorganic salt is separated out from the tail liquid, and wet filter residues and filtrate are obtained through a simple filtering method; the wet filter residue is wet inorganic salt, and the filtrate is organic matter extract liquor; respectively drying the wet filter residue and the filtrate to constant weight at a certain temperature to respectively obtain dried inorganic salt and organic matter, weighing the weight, and calculating the content of the inorganic salt and the organic matter in the tail liquid. The method can realize removal of inorganic salts in the glutamic acid fermentation tail liquid and extraction and content determination of the organic matters, for a glutamic acid granular fertilizer manufacturer, the organic matter content index can be used for predicting the caking condition of the granular fertilizer, and the extracted organic matters can be directly used for liquid chromatography-mass spectrometry tandem instrument sample loading, so that the method is suitable for large-scale industrial production. And carrying out all-component analysis on the organic matters.

This invention belongs to the field of bio-fermentation technology and discloses a method for improving glutamic acid fermentation by using liquid alkali in conjunction with liquid ammonia to regulate pH. The method includes the following steps: when glutamic acid fermentation has reached 6, 7, or 8 hours, pH is adjusted using a combined liquid alkali and liquid ammonia feedstock to control the pH at approximately 7.0 until fermentation is complete. This invention, by employing a liquid ammonia-liquid alkali synergistic regulation strategy in the early stages of fermentation, effectively balances the relationship between nitrogen supply and pH control, thereby improving the level of glutamic acid fermentation.

The invention relates to the field of microorganisms, and discloses corynebacterium glutamicum and application thereof. Corynebacterium glutamicum with the preservation number of CGMCC No.35081 can tolerate the environment with the pH value of 5 and still has glutamic acid fermentation performance, the strain can still have high glutamic acid yield and sugar-acid conversion rate under the acidic condition with the pH value of 5.7-5.9, the performance of the strain is not reduced compared with that under the neutral fermentation condition, and even can be maintained at a considerable level, and the strain can be applied to the field of fermentation. By means of the characteristic, the adding amount of liquid ammonia in the later period of fermentation can be greatly reduced, and therefore raw material consumption and comprehensive production cost in the production process are remarkably reduced.

The present application relates to the technical field of fermentation wastewater in biological fermentation industry, and is a method for preparing coal chemical wastewater flocculant by using glutamic acid fermentation wastewater, which comprises the following steps: extracting and purifying the glutamic acid fermentation wastewater to obtain fermentation wastewater extract; adding a required amount of metal salt and sodium hydroxide into the fermentation wastewater extract to obtain a reaction mixture, grinding the reaction, and removing impurities from the reaction product to obtain the coal chemical wastewater flocculant, wherein the metal salt comprises magnesium salt, calcium salt, copper salt and trivalent iron salt. The present application realizes secondary utilization of the glutamic acid fermentation wastewater, fully utilizes the effective substances in the glutamic acid fermentation wastewater, and achieves excellent treatment effect of the prepared coal chemical wastewater flocculant on coal chemical wastewater. The preparation and treatment processes are both non-toxic, harmless, green and pollution-free, and the effect of turning waste into treasure and achieving more with less is realized.

The invention belongs to the technical field of biological production, and discloses a method for improving glutamic acid fermentation efficiency, which comprises the following steps: glutamic acid is produced by fermentation of glutamic acid producing strains, and a surfactant is added in the fermentation process. The method adopts the surfactant to regulate the fermentation mode, improves the yield of glutamic acid, and is environment-friendly and low in cost.

The application provides a method for recovering pyroglutamic acid from glutamic acid fermentation liquor, which comprises the following steps: removing bacteria bodies from the glutamic acid fermentation liquor through centrifugation; extracting glutamic acid from the supernatant through isoelectric separation after concentration; separating ammonium sulfate from the glutamic acid isoelectric mother liquor through concentration and crystallization; separating through electrodialysis after decolorization and filtration of the secondary mother liquor; collecting concentrated water and dilute water respectively; recycling the dilute water to the isoelectric separation process; and separating ammonium sulfate and pyroglutamic acid through alcohol extraction and filtration of the concentrated water. The method can reduce production cost and provide a new means for effective recovery and utilization of pyroglutamic acid in the monosodium glutamate industry by treating the glutamic acid isoelectric mother liquor through electrodialysis to recover high-value-added product pyroglutamic acid.

The purpose of the present invention is to develop a method for the fermentative production of L-glutamic acid based on nitrogen molecules in the atmosphere using nitrogen-immobilized bacteria. The present invention develops a culture method for maintaining a state in which nitrogen-immobilizing ability is increased in nitrogen-immobilizing bacteria. This culture method is characterized by mixing an organic acid such as citric acid into a nitrogen-restricted medium placed in an open container. A method for producing L-glutamic acid by fermentation depending on nitrogen immobilization can be developed by culturing a gene-altered strain that highly expresses a citrate synthase gene and a citrate transporter gene using this culture method. In this process, a method for producing L-glutamic acid by fermentation that depends on nitrogen immobilization by using a modified strain that highly expresses a citrate synthase gene or a 2-methylcitrate synthase gene can be developed. The present invention relates to a method for producing the microorganism and a method for culturing the microorganism.

The present application relates to monosodium glutamate clean production technology field, specifically to a kind of method for preparing sodium glutamate directly from glutamic acid fermentation liquor using cation replacement dialysis, the method comprises: glutamic acid fermentation liquor is passed into cation replacement dialysis device and sodium ion aqueous solution carry out ammonium ion and sodium ion cation replacement dialysis, finally obtain sodium glutamate and ammonia water or ammonium salt, wherein, the main component of glutamic acid fermentation liquor is ammonium glutamate.The method can realize the direct conversion from ammonium glutamate to sodium glutamate, and the method has the advantages of few process steps, simple flow, environment-friendly and low energy consumption.

The invention belongs to the technical field of biology, and discloses a method for producing L-glutamic acid, which comprises the following steps: fermenting by using a fermentation culture medium containing oligopeptide PSGPR, then obtaining an L-glutamic acid wet crystal and a mother liquor through an evaporation and concentration process, separating and extracting the mother liquor, and recovering again to obtain the L-glutamic acid wet crystal. The method can improve the glutamic acid fermentation efficiency, optimize the separation and purification process and improve the glutamic acid yield.

The invention discloses acid-resistant corynebacterium glutamicum and application thereof, and relates to the technical field of microorganisms. The acid-resistant corynebacterium glutamicum is named as corynebacterium glutamicum A-8, and is preserved in the Guangdong Microbial Culture Collection Center on June 3, 2025, the preservation number is GDMCC NO: 66452, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The Corynebacterium glutamicum A-8 provided by the invention can normally grow in an acidic environment with the pH value of 4.0 and produce glutamic acid, and the problem of bacterial strain growth inhibition caused by pH value reduction in traditional glutamic acid fermentation is effectively solved.

The invention discloses a special culture medium for glutamic acid fermentation in a low-pH environment as well as a preparation method and application thereof, and relates to the technical field of biological fermentation and microbial culture. The culture medium comprises a carbon source compounded by glucose and sorbitol, a nitrogen source compounded by liquid ammonia and double-enzyme hydrolyzed corn steep liquor, inorganic salt with specific composition, trace elements and a glycine-citric acid buffer system. By systematically reconstructing a carbon source, a nitrogen source, inorganic salt, trace elements and a buffer system, the provided culture medium can effectively meet the metabolic demand of corynebacterium glutamicum under the acidic stress of pH 4.8-5.5, and the problems of carbon and nitrogen source utilization obstacle, key enzyme activity inhibition and intracellular metabolic flow imbalance caused by pH reduction are remarkably relieved; therefore, high-efficiency fermentation under the low-pH condition is realized.

The present application relates to the technical field of glutamic acid production, and particularly relates to a method for directly extracting glutamic acid from glutamic acid fermentation liquor by cation replacement dialysis, comprising the following steps: (1) cation replacement dialysis: glutamic acid fermentation liquor is introduced into a cation replacement dialysis device and an acid solution to perform cation replacement dialysis treatment, so as to obtain glutamic acid acid conversion liquor and ammonium salt, wherein the main component of the glutamic acid fermentation liquor is ammonium glutamate; (2) glutamic acid crystallization: the glutamic acid acid conversion liquor obtained in step (1) is crystallized to separate glutamic acid crystals, so as to obtain glutamic acid crystals and salt-free liquor. The present application can realize efficient conversion from ammonium glutamate to glutamic acid, and the method can improve the recovery rate of glutamic acid and simultaneously obtain high-purity ammonium salt.

The invention belongs to the technical field of fermentation engineering, and discloses an energy-saving continuous sterilization process of a glutamic acid fermentation culture medium, and the parameters are as follows: the temperature is 130-135 DEG C, the pressure is 230-360 kPa, and the holding time is 10-15 minutes. According to the invention, the problems of serious latent heat loss, poor sterilization effect, high contamination rate, large nutrient loss, high energy consumption and the like in the existing normal-pressure continuous sterilization process are solved, and the purposes of improving the sterilization effect, reducing the contamination rate, reducing the nutrient loss and saving energy are achieved.

The present invention provides a method for decolorizing γ-polyglutamic acid fermentation broth, comprising: four steps of resin decolorization, activated carbon decolorization, glutamic acid sedimentation decolorization, and membrane filtration treatment decolorization. By means of the combined decolorization process of the present invention, the decolorization rate of the γ-polyglutamic acid fermentation broth can reach over 95%, the recovery rate of γ-polyglutamic acid can reach over 92%, the protein removal rate reaches over 67%, and the recovery rate of glutamic acid can reach over 90%. The decolorization method provided by the present invention has a novel route, uses less decolorizing agent, does not use organic solvents, has no high-temperature heating, has a low degree of damage to high-molecular-weight γ-PGA, and can realize the recycling of the fermentation raw material glutamic acid. The present invention is suitable for large-scale industrial production.

The application belongs to the field of biotechnology, and discloses a method for improving glutamic acid yield by feeding a nutrient solution, which comprises the following steps: feeding a nutrient solution containing glucose in a fermentation process. The application reduces the inhibition of cell growth and activity caused by high temperature in the middle and late stages of glutamic acid fermentation.

This invention belongs to the field of bio-fermentation technology and discloses a method for improving glutamic acid fermentation using nutrients, which includes the following steps: adding nutrients containing vitamin V during the fermentation process. A1 Nutrients containing valine. This invention combines vitamin A and valine in glutamic acid fermentation, which can improve strain activity and yield, and further enhance the fermentation efficiency and sugar-acid conversion rate of glutamic acid.

The present application belongs to the technical field of wastewater treatment, and discloses a method for treating glutamic acid fermentation wastewater, which comprises the following steps: mixing bacillus subtilis inoculum and brevitux inoculum uniformly to obtain a compound inoculum, adding the compound inoculum into the glutamic acid fermentation wastewater, and treating for more than 24 hours. The method uses high-efficiency inoculum, has simple compatibility, requires a small amount of use, and has a wide application prospect.