12 results about "Glutamate fermentation" patented technology

The invention relates to the technical field of microbial fermentation, and discloses a fermentation composition with a whitening effect and a preparation method and application thereof. According to the application, Chinese rose, a sodium glutamate culture medium and sprouted mung beans are respectively used as fermentation objects, and are fermented by selecting plant lactobacillus and bacillus subtilis to respectively obtain a Chinese rose fermented product, a glutamic acid fermented product and a mung bean sprout fermented product; compared with the prior art, the arbutin fermented product has the advantages that the arbutin fermented product shows more excellent monophenol enzyme inhibition rate and diphenol enzyme inhibition rate which are superior to those of arbutin, particularly, the effect is optimal when the three fermented products are compounded, and a theoretical basis is provided for development and application of whitening cosmetic raw materials and products.

The invention belongs to the technical field of biology, and discloses a process for improving the fermentation rate of glutamic acid, which comprises the following steps: a fermentation medium containing oligopeptide is used for fermentation, and the amino acid sequence of the oligopeptide contains PSGPR. By optimizing the components of the fermentation culture medium, the density and activity of the strain are improved, so that the glutamic acid fermentation efficiency is promoted to be improved.

This invention belongs to the field of bio-fermentation technology and discloses a method for improving glutamic acid fermentation by using liquid alkali in conjunction with liquid ammonia to regulate pH. The method includes the following steps: when glutamic acid fermentation has reached 6, 7, or 8 hours, pH is adjusted using a combined liquid alkali and liquid ammonia feedstock to control the pH at approximately 7.0 until fermentation is complete. This invention, by employing a liquid ammonia-liquid alkali synergistic regulation strategy in the early stages of fermentation, effectively balances the relationship between nitrogen supply and pH control, thereby improving the level of glutamic acid fermentation.

The invention relates to the field of microorganisms, and discloses corynebacterium glutamicum and application thereof. Corynebacterium glutamicum with the preservation number of CGMCC No.35081 can tolerate the environment with the pH value of 5 and still has glutamic acid fermentation performance, the strain can still have high glutamic acid yield and sugar-acid conversion rate under the acidic condition with the pH value of 5.7-5.9, the performance of the strain is not reduced compared with that under the neutral fermentation condition, and even can be maintained at a considerable level, and the strain can be applied to the field of fermentation. By means of the characteristic, the adding amount of liquid ammonia in the later period of fermentation can be greatly reduced, and therefore raw material consumption and comprehensive production cost in the production process are remarkably reduced.

The present application relates to monosodium glutamate clean production technology field, specifically to a kind of method for preparing sodium glutamate directly from glutamic acid fermentation liquor using cation replacement dialysis, the method comprises: glutamic acid fermentation liquor is passed into cation replacement dialysis device and sodium ion aqueous solution carry out ammonium ion and sodium ion cation replacement dialysis, finally obtain sodium glutamate and ammonia water or ammonium salt, wherein, the main component of glutamic acid fermentation liquor is ammonium glutamate.The method can realize the direct conversion from ammonium glutamate to sodium glutamate, and the method has the advantages of few process steps, simple flow, environment-friendly and low energy consumption.

The invention belongs to the technical field of biology, and discloses a method for producing L-glutamic acid, which comprises the following steps: fermenting by using a fermentation culture medium containing oligopeptide PSGPR, then obtaining an L-glutamic acid wet crystal and a mother liquor through an evaporation and concentration process, separating and extracting the mother liquor, and recovering again to obtain the L-glutamic acid wet crystal. The method can improve the glutamic acid fermentation efficiency, optimize the separation and purification process and improve the glutamic acid yield.

The invention discloses a special culture medium for glutamic acid fermentation in a low-pH environment as well as a preparation method and application thereof, and relates to the technical field of biological fermentation and microbial culture. The culture medium comprises a carbon source compounded by glucose and sorbitol, a nitrogen source compounded by liquid ammonia and double-enzyme hydrolyzed corn steep liquor, inorganic salt with specific composition, trace elements and a glycine-citric acid buffer system. By systematically reconstructing a carbon source, a nitrogen source, inorganic salt, trace elements and a buffer system, the provided culture medium can effectively meet the metabolic demand of corynebacterium glutamicum under the acidic stress of pH 4.8-5.5, and the problems of carbon and nitrogen source utilization obstacle, key enzyme activity inhibition and intracellular metabolic flow imbalance caused by pH reduction are remarkably relieved; therefore, high-efficiency fermentation under the low-pH condition is realized.

The present application relates to the technical field of glutamic acid production, and particularly relates to a method for directly extracting glutamic acid from glutamic acid fermentation liquor by cation replacement dialysis, comprising the following steps: (1) cation replacement dialysis: glutamic acid fermentation liquor is introduced into a cation replacement dialysis device and an acid solution to perform cation replacement dialysis treatment, so as to obtain glutamic acid acid conversion liquor and ammonium salt, wherein the main component of the glutamic acid fermentation liquor is ammonium glutamate; (2) glutamic acid crystallization: the glutamic acid acid conversion liquor obtained in step (1) is crystallized to separate glutamic acid crystals, so as to obtain glutamic acid crystals and salt-free liquor. The present application can realize efficient conversion from ammonium glutamate to glutamic acid, and the method can improve the recovery rate of glutamic acid and simultaneously obtain high-purity ammonium salt.

The invention belongs to the technical field of fermentation engineering, and discloses an energy-saving continuous sterilization process of a glutamic acid fermentation culture medium, and the parameters are as follows: the temperature is 130-135 DEG C, the pressure is 230-360 kPa, and the holding time is 10-15 minutes. According to the invention, the problems of serious latent heat loss, poor sterilization effect, high contamination rate, large nutrient loss, high energy consumption and the like in the existing normal-pressure continuous sterilization process are solved, and the purposes of improving the sterilization effect, reducing the contamination rate, reducing the nutrient loss and saving energy are achieved.

The application belongs to the field of biotechnology, and discloses a method for improving glutamic acid yield by feeding a nutrient solution, which comprises the following steps: feeding a nutrient solution containing glucose in a fermentation process. The application reduces the inhibition of cell growth and activity caused by high temperature in the middle and late stages of glutamic acid fermentation.

This invention belongs to the field of bio-fermentation technology and discloses a method for improving glutamic acid fermentation using nutrients, which includes the following steps: adding nutrients containing vitamin V during the fermentation process. A1 Nutrients containing valine. This invention combines vitamin A and valine in glutamic acid fermentation, which can improve strain activity and yield, and further enhance the fermentation efficiency and sugar-acid conversion rate of glutamic acid.

The present application belongs to the technical field of wastewater treatment, and discloses a method for treating glutamic acid fermentation wastewater, which comprises the following steps: mixing bacillus subtilis inoculum and brevitux inoculum uniformly to obtain a compound inoculum, adding the compound inoculum into the glutamic acid fermentation wastewater, and treating for more than 24 hours. The method uses high-efficiency inoculum, has simple compatibility, requires a small amount of use, and has a wide application prospect.