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234 results about "Glutamate fermentation" patented technology

Two pathways are involved in the fermentation of glutamate to acetate, butyrate, carbon dioxide, and ammonia-the methylaspartate and the hydroxyglutarate pathways which are used by Clostridium tetanomorphum and Peptococcus aerogenes, respectively.

Novel production method for glutamic acid

The invention discloses a novel production method for glutamic acid, belonging to the technical field of the production of amino acid. The novel production method for the glutamic acid comprises the following steps of: removing thalli and insolubles by means of high-speed disc separation; evaporating and concentrating separated glutamic acid material liquid through a multi-effect plate type evaporator at low temperature, wherein the generated secondary steam condensed water is used for fermentation ingredients of the glutamic acid; performing continuous isoelectric extraction on the glutamic acid in the evaporated glutamic acid concentrated solution; absorbing the glutamic acid by making supernatant fluid pass through ion exchange columns; performing isoelectric reextraction on the analyzed glutamic acid; inputting high-concentration wastewater into a fertilizer workshop for producing fertilizer; squeezing heavy phase (mycoprotein) through a plate frame, and granulating; and drying through a fluid bed, and thus producing high-protein feed. The novel production method for the glutamic acid has the advantages of low unit consumption of liquid ammonia and sulfuric acid, high extraction yield of the glutamic acid, less ion exchange investment and the like; and meanwhile, the purity of the extracted glutamic acid is high, sodium glutamate can be produced without crystalloblast, resources are fully used in the whole process, the aims of energy conservation and consumption reduction are achieved, and the novel production method for the glutamic acid has a wide application prospect.
Owner:HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD

Amino acid fermentation bacteria utilization method

The invention discloses an amino acid fermentation bacteria utilization method, which belongs to the technical field of amino acid production. The method comprises the steps that by taking glutamic acid fermentation as an example and adopting the isoelectric point of fermentation broth, a disc separator is utilized to separate mycoprotein in the fermentation broth, and a moderate amount of compound enzyme is added into the mycoprotein serum, so that the walls of bacteria are broken and the bacteria are enzymetically hydrolyzed. The enzymatic hydrolysate is separated by the high-speed disc separator, and the cell walls are removed, the obtained clarified enzymatic hydrolysate is concentrated under low temperature to produce the enzymetically hydrolyzed mycoprotein extract, or the clarified enzymatic hydrolysate is dried by a gunite granulation fluidized bed drier, a spray granulation drier and the like to produce the enzymetically hydrolyzed mycoprotein powder. In the method, the utilization value of the mycoprotein is greatly increased, so that the mycoprotein is changed from cheap feed into high value-added yeast extract and yeast powder substitute, the product can be used as fermentation ingredient and in other fields, the resources can be sufficiently utilized in the whole process, the purposes of energy saving and consumption reduction are achieved, and the invention has a broad application prospect.
Owner:HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD

Method for increasing acid production rate and extracting rate of glutamic acid

The invention belongs to the technical field of amino acids and discloses a method for increasing an acid production rate and an extracting rate of glutamic acid. The method for increasing the acid production rate and the extracting rate of the glutamic acid comprises the following steps: putting a brevibacterium flavum seed solution for producing the glutamic acid into a fermentation tank filledwith fermentation medium to carry out fermentation cultivation; when the brevibacterium flavum seed solution is fermented for 24 hours, separating out a fermentation solution in the fermentation tankvia a ceramic membrane; draining a filter liquor; putting concentrated thalli back into the fermentation tank; and meanwhile, supplementing a fermentation medium B to the fermentation tank; and continuing to carry out fermentation for 16 h to finish fermentation. The method for increasing the acid production rate and the extracting rate of the glutamic acid, disclosed by the invention, has the advantages that the glutamic acid fermentation process is more stable and easy to control by optimizing a culture medium; and moreover, the yield and glucose conversion rate of the glutamic acid are increased, the quality of the fermentation solution is improved, the glutamic acid extracting cost is reduced, and the comprehensive benefits are increased.
Owner:HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD +2

Method for preparing glutamic acid fermentation organic nitrogen additive from glutamic acid fermentation waste thalli

The invention relates to a method for preparing a glutamic acid fermentation organic nitrogen additive from glutamic acid fermentation waste thalli, which solves the problem that environment can be polluted by directly discharging fermented waste thalli or economic benefit is low when the fermented waste thalli are prepared into a feed additive in the conventional glutamic acid production. The glutamic acid fermentation waste thalli are taken as raw materials; protein hydrolyzate is obtained by enzymatic hydrolysis, acid hydrolysis or an enzymatic hydrolysis and acid hydrolysis combined method; and the hydrolyzate is concentrated or dried to prepare the organic nitrogen additive for fermentation. For the organic nitrogen additive prepared by the method, the protein recovery rate of the glutamic acid fermentation waste thalli reaches 93 percent; the prepared organic nitrogen additive can be recycled for glutamic acid fermentation and replaces yeast extract, peptone, corn steep liquor and other organic nitrogen additives which are used in the conventional glutamic acid production, so environmental pollution can be reduced and the added value of the organic nitrogen additive can be greatly improved; and the organic nitrogen additive has great significance for the comprehensive utilization of the glutamic acid fermentation waste thalli.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY

Method for producing high-yield gamma-propalanine and application thereof

The invention relates to a method for producing high-yield gamma-propalanine and an application thereof, belonging to the technical field of biotechnology medicine and food engineering. The method comprises the following steps: carrying out strain separated screening, acridine orange-ultraviolet ray mutagenesis and N<+> injection mutagenesis to obtain a high-yield gamma-propalanine LactobacillusbrevisTCCC (CGMCC No.3414) strain; optimizing a fermentation medium and fermentation conditions; coupling fermentation of the gonotokont of the strain with resting cell biotransformation of the strain to produce the gamma-propalanine; employing the membrane filtration technology, the adsorbent resin decoloration technology, the strong acid resin cation exchange technology, the ethanol recrystallization technology, the preparative chromatography technology and the energy-saving and cost-reducing technology to separate the gamma-propalanine from the fermentation fluid and the biotransformation fluid; and then carrying out purification to obtain the crystallized gamma-propalanine with the purity of 99%. The method has the advantages of cheap raw materials, low energy consumption for production, low production cost, good product security and easy realization of industrialized production. Applied to glutamic acid fermentation waste liquor for producing the gamma-propalanine, the strain has good potential for generating social and economic benefits.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY

Monosodium glutamate production technology

The invention discloses a monosodium glutamate production technology which comprises the following steps: fermenting glutamic acid; extracting glutamic acid: a glutamic acid fermentation broth is subjected to pasteurization and two-stage ultrafiltration to obtain bacteria paste and a glutamic acid clear solution, the glutamic acid clear solution is subjected to evaporation and concentration, and is separated after continuously isoelectric cooling to obtain crystallized alpha-glutamic acid and a separated mother solution; mixing, heating and keeping warm and separating alpha-glutamic acid and a crystal transformation mother solution to obtain the glutamic acid, preparing a suspension liquid through mixing the glutamic acid and water, controlling the crystal transformation pH value to be 4.0-5.0, rising the temperature, carrying out the crystal transformation to obtain beta-glutamic acid, neutralizing with a sodium carbonate water solution, then performing activated carbon decolorizing, filtering, carrying out decoloration and iron removal on the filtrate through a granular activated carbon column, performing evaporative crystallization and centrifugal separation on the decoloring solution, drying and screening to obtain the finished product. The monosodium glutamate production technology has the advantages that the discharge of waste liquid during the monosodium glutamate production process is reduced, the consumption of chemicals is reduced, the production cost is reduced, the economic benefit is improved, and the resource recycling is facilitated.
Owner:广州奥桑味精食品有限公司

Method for preparing gamma-polyglutamic acid through soild fermentation by utilizing edible fungi residues

The invention discloses a method for preparing gamma-polyglutamic acid through solid fermentation by utilizing edible fungi residues. The method comprises the following steps: (1) preparation of solid fungicide: collecting thallus in gamma- polyglutamic acid fermentation liquor to be mixed with a carrier, and drying to obtain the solid fungicide; (2) solid fermentation: inoculating the solid fungicide obtained from the step (1) into a fermentation medium to be mixed uniformly, and fermenting to prepare the gamma-polyglutamic acid, wherein the fermentation medium consists of a solid matrix and an exogenous additive, and the solid matrix is a mixture of the edible fungi residues and aginomoto. Compared with the prior art in which gamma-polyglutamic acid is produced through solid fermentation, the method disclosed by the invention is used for efficiently producing gamma-polyglutamic acid, the production cost is greatly reduced through the utilization of agricultural waste residues, meanwhile, a new way is found for the disposal of the residues, and the environment pollution problem caused by the residues is avoided. Therefore, the method is a high-efficient, low-cost, environment-friendly biological reaction process and has good industrial application prospect.
Owner:轩凯生物科技(山东)有限公司

Special strawberry composite biological fertilizer as well as preparation method and application thereof

The invention relates to a special strawberry composite biological fertilizer as well as a preparation method and application thereof. The special strawberry composite biological fertilizer comprisesthe following components: EDTA-Ca (Ethylene Diamine Tetraacetic Acid), magnesium sulfate heptahydrate, EDTA-Mg, heptahydrate, EDTA-Zn, manganese sulfate monohydrate, EDTA-Mn, boric acid, EDTA-Fe, EDTA-Cu, soybean molasses, polyglutamic acid fermentation broth, chitosan oligosaccharide, algin, compound amino acid, humic acid, bacillus thuringiensis fermentation broth, bacillus megatherium fermentation broth, bacillus mucilaginosus fermentation broth and streptomyces jingyangensis fermentation broth. Through bacterium activation, seed liquid preparation, fermentation culture and compounding, thecomposite biological fertilizer is prepared, the composite biological fertilizer is applied to strawberry planting, and a diluted composite biological fertilizer liquid is sprayed at different growthstages of strawberry respectively. Due to synergetic effects of substances such as inorganic salts, organic matters and microorganisms, long lasting nutrient substances are provided to strawberry crops, absorption of nutrients in soil by plants is promoted, the fertilizer amount is reduced, and the chemical fertilizer cost is reduced.
Owner:天津北洋百川生物技术有限公司

Fertilizer synergistic additive and preparation thereof

The invention relates to technology of synergy for fertilizers, and more specifically relates to a fertilizer synergistic additive and a preparation thereof. The solid dosage form synergistic additive comprises the following raw materials in percentage by weight: 55-93.5% of fermentation broth of polyglutamic acid, 0.5-3% of thiourea, 0.5-9% of N-(n-butyl)thiophosphoric triamide, 5-30% of dicyandiamide, and 48-91% of diatomite. Or the liquid dosage form synergistic additive comprises the following raw materials in percentage by weight: 55-93.5% of fermentation broth of polyglutamic acid, 0.5-3% of thiourea, 0.5-9% of N-(n-butyl)thiophosphoric triamide, 5-30% of dicyandiamide, and 0.5-3% of an emulsifier. The fermentation broth contains 3-10% of polyglutamic acid, and the emulsifier is polyoxyethylene sorbitan fatty acid ester. The fertilizer synergistic additive can increase effective concentration of fertilizer nutrients, prolong effective period of the fertilizer, promote crops to absorb and transport nutrients, overcome the problem of insufficient release of the long-acting slow-release fertilizer nutrients at early stage, guarantee efficiency of fertilizer at later period, the validity period of fertilizer nutrients is longer than 120 days, so that long-acting and high-efficiency utilization of fertilizer nutrients are realized.
Owner:辽宁中科生物工程股份有限公司
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