29 results about "Glutamate fermentation" patented technology

The application discloses a preparation method of glutamic acid bacterial protein ecological fertilizer, and the preparation steps are as follows: firstly, zinc polysilicate is added into glutamic acid fermentation waste liquid, and bacterial protein-zinc polysilicate complex is obtained through filtration; then, zinc silicate and hydroxyapatite are added, and amino acid composite hydroxyapatite particles are formed through the action of citric acid; and finally, urea-humic acid-dipotassium hydrogen phosphate complex is sprayed on the amino acid composite hydroxyapatite particles to obtain the ecological fertilizer. The ecological fertilizer can not only fully utilize the bacterial protein in the glutamic acid fermentation waste liquid, but also play a good slow-release effect of nitrogen, phosphorus and other elements through the action of two complexes. In addition, the addition of silicon and zinc elements can play a supplementing effect for the growth of crops, so that the fertilizer has the dual functions of environmental protection and long-acting slow release, and has a good promoting effect on the growth of crops.

The invention relates to the technical field of microbial fermentation, and discloses a fermentation composition with a whitening effect and a preparation method and application thereof. According to the application, Chinese rose, a sodium glutamate culture medium and sprouted mung beans are respectively used as fermentation objects, and are fermented by selecting plant lactobacillus and bacillus subtilis to respectively obtain a Chinese rose fermented product, a glutamic acid fermented product and a mung bean sprout fermented product; compared with the prior art, the arbutin fermented product has the advantages that the arbutin fermented product shows more excellent monophenol enzyme inhibition rate and diphenol enzyme inhibition rate which are superior to those of arbutin, particularly, the effect is optimal when the three fermented products are compounded, and a theoretical basis is provided for development and application of whitening cosmetic raw materials and products.

The invention discloses an extraction method of high-purity glutamic acid, and belongs to the field of monosodium glutamate production industry. The method comprises the following steps: introducing glutamic acid fermentation liquor into an ultrafiltration membrane to obtain clear liquor and thallus concentrated liquor A; the thallus concentrated solution A enters a dialysis membrane to obtain dialysate and a thallus concentrated solution B; carrying out filter pressing on the thallus concentrated solution B to obtain fungi residues and filtrate; merging the filtrate and the thallus concentrated solution A; the dialysate enters a concentration membrane, a concentrated solution and membrane produced water are obtained, and the membrane produced water is dialysis water; the concentrated solution and the clear solution enter a continuous isoelectric crystallizer to be crystallized, and glutamic acid crystal slurry is discharged at the same time for crystallization; performing primary separation to obtain glutamic acid and isoelectric mother liquor A; the isoelectric mother liquor A is subjected to secondary separation, fine glutamic acid crystals and isoelectric mother liquor B are obtained, and the isoelectric mother liquor B enters a sewage treatment procedure; and fine glutamic acid crystals return to the continuous isoelectric crystallizer. The glutamic acid obtained by the method has the advantages of high yield, high product purity, high light transmittance and good color and luster, and is superior to the commonly used'concentration continuous isoelectric crystallization + crystal transformation 'process.

The invention belongs to the technical field of biology, and discloses a process for improving the fermentation rate of glutamic acid, which comprises the following steps: a fermentation medium containing oligopeptide is used for fermentation, and the amino acid sequence of the oligopeptide contains PSGPR. By optimizing the components of the fermentation culture medium, the density and activity of the strain are improved, so that the glutamic acid fermentation efficiency is promoted to be improved.

The present invention belongs to the technical field of fertilizer production, and discloses a fertilizer production method and system containing glutamic acid fermentation waste liquid, the method includes soil conditioner production and organic-inorganic compound fertilizer production, and the fertilizer production system containing glutamic acid fermentation waste liquid is provided with a detection terminal for detecting fertilizer composition; a soil conditioner device connected to the detection terminal for realizing soil conditioning; and an organic-inorganic compound fertilizer preparation device connected to the detection terminal for realizing fertilizer conditioning. By adding 200-300 mesh primary granulation fertilizer powder from the initial batching, adjusting the proportion of granulation liquid in dry matter, especially the proportion of fine particle organic matter, improving material conditions, thereby effectively reducing the spraying granulation temperature, shortening the residence time of the liquid in the granulator, and then effectively improving the solubility of the final product.

The invention belongs to the technical field of separation and extraction of organic matters in high-salt-content wastewater, and discloses a method for extracting organic matters in glutamic acid fermentation tail liquid and determining the content of the organic matters in the glutamic acid fermentation tail liquid and an extracting agent.The high-concentration glutamic acid fermentation tail liquid is filtered firstly and then mixed with the extracting agent.The high-concentration glutamic acid fermentation tail liquid is mixed with the extracting agent.The inorganic salt is separated out from the tail liquid, and wet filter residues and filtrate are obtained through a simple filtering method; the wet filter residue is wet inorganic salt, and the filtrate is organic matter extract liquor; respectively drying the wet filter residue and the filtrate to constant weight at a certain temperature to respectively obtain dried inorganic salt and organic matter, weighing the weight, and calculating the content of the inorganic salt and the organic matter in the tail liquid. The method can realize removal of inorganic salts in the glutamic acid fermentation tail liquid and extraction and content determination of the organic matters, for a glutamic acid granular fertilizer manufacturer, the organic matter content index can be used for predicting the caking condition of the granular fertilizer, and the extracted organic matters can be directly used for liquid chromatography-mass spectrometry tandem instrument sample loading, so that the method is suitable for large-scale industrial production. And carrying out all-component analysis on the organic matters.

This invention belongs to the field of bio-fermentation technology and discloses a method for improving glutamic acid fermentation by using liquid alkali in conjunction with liquid ammonia to regulate pH. The method includes the following steps: when glutamic acid fermentation has reached 6, 7, or 8 hours, pH is adjusted using a combined liquid alkali and liquid ammonia feedstock to control the pH at approximately 7.0 until fermentation is complete. This invention, by employing a liquid ammonia-liquid alkali synergistic regulation strategy in the early stages of fermentation, effectively balances the relationship between nitrogen supply and pH control, thereby improving the level of glutamic acid fermentation.

The invention relates to the field of microorganisms, and discloses corynebacterium glutamicum and application thereof. Corynebacterium glutamicum with the preservation number of CGMCC No.35081 can tolerate the environment with the pH value of 5 and still has glutamic acid fermentation performance, the strain can still have high glutamic acid yield and sugar-acid conversion rate under the acidic condition with the pH value of 5.7-5.9, the performance of the strain is not reduced compared with that under the neutral fermentation condition, and even can be maintained at a considerable level, and the strain can be applied to the field of fermentation. By means of the characteristic, the adding amount of liquid ammonia in the later period of fermentation can be greatly reduced, and therefore raw material consumption and comprehensive production cost in the production process are remarkably reduced.

The invention discloses a method for preparing a fertilizer from glutamic acid fermentation liquor under the assistance of microwaves, and relates to the technical field of fertilizer preparation, and the method has the advantages that the purity of the fertilizer is improved by adopting various materials and methods, and a filter screen coated with a polytetrafluoroethylene nano coating in a multi-stage filtering mode is adopted, so that the utilization rate of the fertilizer is improved. The low surface energy characteristic of polytetrafluoroethylene is utilized, grease impurities and macromolecular protein impurities are effectively repelled, a ceramic membrane filtering device further removes small-particle impurities, the purity of fermentation liquor is ensured, through specific cellulase, hemicellulase and laccase treatment, the cellulose and hemicellulose structures of the corn straw are changed, and the corn straw fermentation liquor is obtained. According to the preparation method, a substance with rich pore structures is formed, then the substance is activated by a low-temperature plasma technology, surface active sites are increased, the adsorption capacity to impurities is remarkably enhanced, the purity of the fertilizer is greatly improved through the synergistic effect of the materials, and the high quality of a fertilizer product is guaranteed.

The invention relates to the technical field of fermentation wastewater in the biological fermentation industry, in particular to a method for preparing a coal chemical wastewater flocculant from glutamic acid fermentation waste liquid, which comprises the following steps: extracting and purifying the glutamic acid fermentation waste liquid to obtain a fermentation waste liquid extract; the required amount of metal salt and sodium hydroxide are added into the fermentation waste liquid extract, a reaction mixture is obtained, grinding reaction and impurity removal of a reaction product are performed, the coal chemical industry wastewater flocculant is obtained, and the metal salt comprises magnesium salt, calcium salt, copper salt and ferric iron salt. The glutamic acid fermentation waste liquid is secondarily utilized, effective substances in the glutamic acid fermentation waste liquid are fully utilized, the prepared coal chemical industry wastewater flocculant achieves an excellent treatment effect on the coal chemical industry wastewater, the preparation and treatment processes are non-toxic, harmless, green and pollution-free, and the effects of turning waste into wealth and getting twofold results with half the effort are achieved.

The present application relates to the technical field of fermentation wastewater in biological fermentation industry, and is a method for preparing coal chemical wastewater flocculant by using glutamic acid fermentation wastewater, which comprises the following steps: extracting and purifying the glutamic acid fermentation wastewater to obtain fermentation wastewater extract; adding a required amount of metal salt and sodium hydroxide into the fermentation wastewater extract to obtain a reaction mixture, grinding the reaction, and removing impurities from the reaction product to obtain the coal chemical wastewater flocculant, wherein the metal salt comprises magnesium salt, calcium salt, copper salt and trivalent iron salt. The present application realizes secondary utilization of the glutamic acid fermentation wastewater, fully utilizes the effective substances in the glutamic acid fermentation wastewater, and achieves excellent treatment effect of the prepared coal chemical wastewater flocculant on coal chemical wastewater. The preparation and treatment processes are both non-toxic, harmless, green and pollution-free, and the effect of turning waste into treasure and achieving more with less is realized.

The invention belongs to the technical field of biological production, and discloses a method for improving glutamic acid fermentation efficiency, which comprises the following steps: glutamic acid is produced by fermentation of glutamic acid producing strains, and a surfactant is added in the fermentation process. The method adopts the surfactant to regulate the fermentation mode, improves the yield of glutamic acid, and is environment-friendly and low in cost.

The application discloses an acid-resistant Corynebacterium glutamicum and application thereof, and relates to the technical field of microorganisms. The acid-resistant Corynebacterium glutamicum is named Corynebacterium glutamicum (Corynebacterium glutamicum) Corynebacterium glutamicum ) A-8, and is preserved in the Guangdong Microbial Culture Collection Center on June 3, 2025, with a preservation number of GDMCC NO: 66452 and a preservation address of 5th Floor, Building 59, 100, Middle Liangma Street, Guangzhou. The Corynebacterium glutamicum (Corynebacterium glutamicum) Corynebacterium glutamicum ) A-8 provided in the application can grow normally and produce glutamic acid in an acidic environment with a pH of 4.0, and effectively solves the problem of growth inhibition of a strain caused by pH reduction in traditional glutamic acid fermentation.

The invention discloses a pretreatment process for preparing a liquid fertilizer by using glutamic acid fermentation liquor, and relates to the technical field of liquid fertilizer preparation, a bacillus subtilis culture solution is prepared, the culture solution is treated, bacillus subtilis thalli are collected, then a nitrogen mustard chemical mutagenic agent is used for treating bacillus subtilis, and the liquid fertilizer is prepared. The method has the advantages that a large number of adsorption sites are provided for the glutamic acid, the adsorption capacity of the glutamic acid is greatly improved, the adsorption capacity of the glutamic acid is greatly improved, and the utilization rate of the glutamic acid is increased. The concentrated sulfuric acid modified high-molecular polymer into which a sulfonic active group is introduced can be subjected to specific chemical bonding with glutamic acid, so that impurities in the fermentation liquor are effectively removed, the adsorption capacity to glutamic acid is enhanced, the purity of the liquid fertilizer is improved, and the utilization value of the glutamic acid fermentation liquor is greatly improved.

The application provides a method for recovering pyroglutamic acid from glutamic acid fermentation liquor, which comprises the following steps: removing bacteria bodies from the glutamic acid fermentation liquor through centrifugation; extracting glutamic acid from the supernatant through isoelectric separation after concentration; separating ammonium sulfate from the glutamic acid isoelectric mother liquor through concentration and crystallization; separating through electrodialysis after decolorization and filtration of the secondary mother liquor; collecting concentrated water and dilute water respectively; recycling the dilute water to the isoelectric separation process; and separating ammonium sulfate and pyroglutamic acid through alcohol extraction and filtration of the concentrated water. The method can reduce production cost and provide a new means for effective recovery and utilization of pyroglutamic acid in the monosodium glutamate industry by treating the glutamic acid isoelectric mother liquor through electrodialysis to recover high-value-added product pyroglutamic acid.

The purpose of the present invention is to develop a method for the fermentative production of L-glutamic acid based on nitrogen molecules in the atmosphere using nitrogen-immobilized bacteria. The present invention develops a culture method for maintaining a state in which nitrogen-immobilizing ability is increased in nitrogen-immobilizing bacteria. This culture method is characterized by mixing an organic acid such as citric acid into a nitrogen-restricted medium placed in an open container. A method for producing L-glutamic acid by fermentation depending on nitrogen immobilization can be developed by culturing a gene-altered strain that highly expresses a citrate synthase gene and a citrate transporter gene using this culture method. In this process, a method for producing L-glutamic acid by fermentation that depends on nitrogen immobilization by using a modified strain that highly expresses a citrate synthase gene or a 2-methylcitrate synthase gene can be developed. The present invention relates to a method for producing the microorganism and a method for culturing the microorganism.

The present application relates to monosodium glutamate clean production technology field, specifically to a kind of method for preparing sodium glutamate directly from glutamic acid fermentation liquor using cation replacement dialysis, the method comprises: glutamic acid fermentation liquor is passed into cation replacement dialysis device and sodium ion aqueous solution carry out ammonium ion and sodium ion cation replacement dialysis, finally obtain sodium glutamate and ammonia water or ammonium salt, wherein, the main component of glutamic acid fermentation liquor is ammonium glutamate.The method can realize the direct conversion from ammonium glutamate to sodium glutamate, and the method has the advantages of few process steps, simple flow, environment-friendly and low energy consumption.

The invention belongs to the technical field of biology, and discloses a method for producing L-glutamic acid, which comprises the following steps: fermenting by using a fermentation culture medium containing oligopeptide PSGPR, then obtaining an L-glutamic acid wet crystal and a mother liquor through an evaporation and concentration process, separating and extracting the mother liquor, and recovering again to obtain the L-glutamic acid wet crystal. The method can improve the glutamic acid fermentation efficiency, optimize the separation and purification process and improve the glutamic acid yield.

The invention discloses acid-resistant corynebacterium glutamicum and application thereof, and relates to the technical field of microorganisms. The acid-resistant corynebacterium glutamicum is named as corynebacterium glutamicum A-8, and is preserved in the Guangdong Microbial Culture Collection Center on June 3, 2025, the preservation number is GDMCC NO: 66452, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The Corynebacterium glutamicum A-8 provided by the invention can normally grow in an acidic environment with the pH value of 4.0 and produce glutamic acid, and the problem of bacterial strain growth inhibition caused by pH value reduction in traditional glutamic acid fermentation is effectively solved.

The invention discloses a special culture medium for glutamic acid fermentation in a low-pH environment as well as a preparation method and application thereof, and relates to the technical field of biological fermentation and microbial culture. The culture medium comprises a carbon source compounded by glucose and sorbitol, a nitrogen source compounded by liquid ammonia and double-enzyme hydrolyzed corn steep liquor, inorganic salt with specific composition, trace elements and a glycine-citric acid buffer system. By systematically reconstructing a carbon source, a nitrogen source, inorganic salt, trace elements and a buffer system, the provided culture medium can effectively meet the metabolic demand of corynebacterium glutamicum under the acidic stress of pH 4.8-5.5, and the problems of carbon and nitrogen source utilization obstacle, key enzyme activity inhibition and intracellular metabolic flow imbalance caused by pH reduction are remarkably relieved; therefore, high-efficiency fermentation under the low-pH condition is realized.

The present application relates to the technical field of glutamic acid production, and particularly relates to a method for directly extracting glutamic acid from glutamic acid fermentation liquor by cation replacement dialysis, comprising the following steps: (1) cation replacement dialysis: glutamic acid fermentation liquor is introduced into a cation replacement dialysis device and an acid solution to perform cation replacement dialysis treatment, so as to obtain glutamic acid acid conversion liquor and ammonium salt, wherein the main component of the glutamic acid fermentation liquor is ammonium glutamate; (2) glutamic acid crystallization: the glutamic acid acid conversion liquor obtained in step (1) is crystallized to separate glutamic acid crystals, so as to obtain glutamic acid crystals and salt-free liquor. The present application can realize efficient conversion from ammonium glutamate to glutamic acid, and the method can improve the recovery rate of glutamic acid and simultaneously obtain high-purity ammonium salt.

The invention belongs to the technical field of fermentation engineering, and discloses an energy-saving continuous sterilization process of a glutamic acid fermentation culture medium, and the parameters are as follows: the temperature is 130-135 DEG C, the pressure is 230-360 kPa, and the holding time is 10-15 minutes. According to the invention, the problems of serious latent heat loss, poor sterilization effect, high contamination rate, large nutrient loss, high energy consumption and the like in the existing normal-pressure continuous sterilization process are solved, and the purposes of improving the sterilization effect, reducing the contamination rate, reducing the nutrient loss and saving energy are achieved.

The invention belongs to the technical field of preparation of polyglutamic acid, and particularly relates to a method for preparing polyglutamic acid based on kitchen waste liquid. According to the method, the kitchen waste liquid is used as a raw material, the content of total sugar and glutamic acid in the waste liquid is measured, for the kitchen waste liquid with low content of total sugar and glutamic acid, the glutamic acid concentrated solution is supplemented to improve nutrient components of the culture medium and reduce the cost of the polyglutamic acid culture medium, and resource utilization of the two waste liquids is realized; meanwhile, ammonium sulfate is added to produce a hypertonic environment, inhibit growth of infectious microbes, reduce sterilization energy consumption, stimulate the polyglutamic acid synthesis capability of strains, reduce the viscosity of the fermentation liquor and reduce ventilation stirring energy consumption, and the low-cost polyglutamic acid fermentation liquor is obtained. The fermentation liquor is concentrated to prepare a liquid polyglutamic acid product, the content of ammonium sulfate is further increased, the corrosion resistance of the product is enhanced, degradation of polyglutamic acid is effectively prevented, and zero discharge treatment of waste liquid of the kitchen waste is realized.

The present invention provides a method for decolorizing γ-polyglutamic acid fermentation broth, comprising: four steps of resin decolorization, activated carbon decolorization, glutamic acid sedimentation decolorization, and membrane filtration treatment decolorization. By means of the combined decolorization process of the present invention, the decolorization rate of the γ-polyglutamic acid fermentation broth can reach over 95%, the recovery rate of γ-polyglutamic acid can reach over 92%, the protein removal rate reaches over 67%, and the recovery rate of glutamic acid can reach over 90%. The decolorization method provided by the present invention has a novel route, uses less decolorizing agent, does not use organic solvents, has no high-temperature heating, has a low degree of damage to high-molecular-weight γ-PGA, and can realize the recycling of the fermentation raw material glutamic acid. The present invention is suitable for large-scale industrial production.

The application belongs to the field of biotechnology, and discloses a method for improving glutamic acid yield by feeding a nutrient solution, which comprises the following steps: feeding a nutrient solution containing glucose in a fermentation process. The application reduces the inhibition of cell growth and activity caused by high temperature in the middle and late stages of glutamic acid fermentation.

This invention belongs to the field of bio-fermentation technology and discloses a method for improving glutamic acid fermentation using nutrients, which includes the following steps: adding nutrients containing vitamin V during the fermentation process. A1 Nutrients containing valine. This invention combines vitamin A and valine in glutamic acid fermentation, which can improve strain activity and yield, and further enhance the fermentation efficiency and sugar-acid conversion rate of glutamic acid.

The present application belongs to the technical field of wastewater treatment, and discloses a method for treating glutamic acid fermentation wastewater, which comprises the following steps: mixing bacillus subtilis inoculum and brevitux inoculum uniformly to obtain a compound inoculum, adding the compound inoculum into the glutamic acid fermentation wastewater, and treating for more than 24 hours. The method uses high-efficiency inoculum, has simple compatibility, requires a small amount of use, and has a wide application prospect.

The invention discloses a preparation method of a glutamic acid mycoprotein ecological fertilizer, which comprises the following steps: firstly, adding polysilicate zinc into glutamic acid fermentation waste liquid, and filtering to obtain a mycoprotein-polysilicate zinc complex; zinc silicate and hydroxyapatite are added, and amino acid composite hydroxyapatite particles are formed under the action of citric acid; and spraying the urea-humic acid-dipotassium phosphate complex onto the amino acid composite hydroxyapatite particles to obtain the ecological fertilizer. According to the ecological fertilizer, mycoprotein in the glutamic acid fermentation waste liquid can be fully utilized, a good slow release effect of nitrogen, phosphorus and other elements can be achieved through the effects of the two complexes, and in addition, the addition of silicon and zinc elements can achieve a supplement effect on the growth of crops; therefore, the fertilizer provided by the invention has dual effects of environmental protection and long-acting slow release, and has a good promotion effect on the growth of crops.

The invention provides a method for decoloring gamma-polyglutamic acid fermentation liquor. The method comprises four steps of resin decoloring, activated carbon decoloring, glutamic acid sedimentation decoloring and membrane filtration treatment decoloring. Through the combined decolorization process, the decolorization rate of the gamma-polyglutamic acid fermentation liquor can reach 95% or above, the recovery rate of gamma-polyglutamic acid can reach 92% or above, the protein removal rate can reach 67% or above, and the recovery rate of glutamic acid can reach 90% or above. The decolorizing method provided by the invention has the advantages of novel route, less decolorizing agent dosage, no use of organic solvent, no high-temperature heating, low damage degree of high-molecular-weight gamma-PGA, realization of cyclic utilization of the fermentation raw material glutamic acid, and suitableness for large-scale industrial production.