99 results about "Poly(gamma-glutamic acid)" patented technology

Methods for producing high molecular weight poly-gamma-glutamic acid (PGA). The PGA is produced by fermentation, and purified by use of tangential flow filtration, followed by diafiltration, as necessary, to yield a product of the desired purity. Product obtained may be of very high purity using all the prescribed purification steps. Product of this purity is suitable for in vivo medical applications. Other applications, such as food or agricultural, may utilize lower purity levels, and hence do not require all the purification steps specified.

The invention relates to a poly-gamma-glutamic acid synergistic fertilizer for strengthening fertilizer effect, which belongs to the fertilizer field. The invention comprises graininess fertilizer which comprises the poly-gamma-glutamic acid. The contents of the poly-gamma-glutamic acid and fertilizer in weight are as follows: 0.01 to 1 percent of the poly-gamma-glutamic acid and the remained content is fertilizer. The contents of poly-gamma-glutamic acid of the invention are strictly controlled and the poly-gamma-glutamic acid can be conveniently fertilized. The poly-gamma-glutamic acid is combined with to make full use of the feature as absorption promoter of fertilizer of the poly-gamma-glutamic acid, therefore, crops output is further raised. The fertilizer is released slowly and prolonged by around 4 times compared with the traditional fertilizer in soil. The invention promotes crop absorption and inhibits fertilizer ingredient decomposition and loss, therefore, fertilizer use is directly decreased by more than 20 percent. The pollution to environment is reduced without excessive or inappropriate fertilization.

The invention discloses a bacillus licheniformis strain which is preserved as bacillus licheniformis WX-02 with the preserving number of NO: M208065 and the preserving date of April 24th, 2008 and the preserving unit of CCTCC. The invention also discloses an application of the bacillus licheniformis strain and a method for producing poly-gamma-glutamic acid by using the bacillus licheniformis strain. Poly-gamma-glutamic acid products are produced by processes of seed solution preparation and liquid submerged fermentation, and the strain has stable genetic performance of the strain, high production efficiency and low production cost. When the bacillus licheniformis strain of the invention and the fermentation method thereof are used for preparing 3000L of poly-gamma-glutamic acid, the yield of a fermentation tank can reach 35.05g / l.

The present invention discloses a strain capable of producing poly-gamma-glutamic acid and a method for producing poly-gamma-glutamic acid by utilizing said strain. The described strain is X-003, a Bacillussubilis, CCTCC, NO:M202048. It adopts seed liquor preparation and liquid submerged fermentation process to produce and obtain the poly-gamma-glutamic acid product. The hereditary feature of said strain is stable, and its capacity for synthesizing poly-gamma-glutamic acid is strong, and the molecular weight of synthetic poly-gamma-glutamic acid can be up to above 10000 KD. The shake-flask fermentation level of poly-gamma-glutamic acid by utilizing said invented strain and its fermentation method can be up to 10 mg / ml, and the fermentation level of the fermentation tank with 80L is 30 mg / ml.

The present invention discloses poly-gamma-glutamic acid preparing process. The process includes the steps of culturing different kinds of poly-gamma-glutamic acid producing bacterial strains in culture medium containing carbon source, nitrogen source, NaCl and intermediate to obtain poly-gamma-glutamic acid fermenting liquor, precipitating in organic solvent, dialysis and drying to obtain poly-gamma-glutamic acid product. The process has the intermediate as the precursor for preparing poly-gamma-glutamic acid, and is simple, low in cost and high in yield.

The invention relates to a bacillus licheniformis engineering bacterium for efficiently synthetizing poly-gamma-glutamic acid, and belongs to the field of microbial metabolism engineering. A conventional molecular biological technique is used; on the basis of bacillus licheniformis WX-02 stored in a laboratory, a double-ingredient regulation and control factor degU is expressed in an enhanced way through external sources; the bacillus licheniformis engineering bacterium WX-02 / pHY-degU capable of efficiently synthetizing the poly-gamma-glutamic acid under the condition of no addition of an external source of glutamic acid is obtained. Liquid fermentation proves that the concentration of the poly-gamma-glutamic acid at a fermentation end point reaches 32.78 g / L; compared with that of an original bacterial strain of bacillus licheniformis WX 02, the concentration is improved by 47.59 percent; the synthesis capability of the poly-gamma-glutamic acid is obviously enhanced. The bacterial strain is preserved in CCTCC (China Center For Type Culture Collection) on May 27, 2016, and the preservation number is CCTCC NO:M2016294.

The invention relates to the application of the gamma-polyglutamic acid as the absorption promoting agent of the fertilizer in the agricultural husbandry. The said gamma-polyglutamic acid is a high molecular polymer of D-glutacid and L- glutacid formed by the alpha-amino and the gamma-carboxyl in the manner of peptide bond and there is also a dissociative carboxyl on the alpha-carbon atom of every repetitive unit with average molecular weight of 10-10,000 kilodaltons. The said gamma-polyglutamic acid can be used as the absorption promoting agent of the fertilizer made of single element of nitrogen, phosphor, kalium or their composition. The significance of the invention is: it can be degraded by edaphon and its environmental value is much better in relation to TPA; it can reduce by 10-30 % of the normal usage of the chemical fertilizer by applying 10-50 grams of fertilizer per area and increase by 10-30 % of the crop yield, at the same time it can improve the crop quality and increase the crop content of nitrogen, phosphor and kalium.

The present invention belongs to the technical field of agricultural microbe, and specifically relates to a microbial capsule preparation taking poly-gamma-glutamic acid as a wall material, a preparation method thereof, and applications thereof in preparing microbial pesticides and microbial fertilizers. The microbial microcapsules taking the poly-gamma-glutamic acid as the wall material have good mechanical strength and flexibility, can be applied in microbial embedding medium for good sustained-release ability, can prevent microbial cells from injuries of ultraviolet rays, temperature, acid or base, ionic strength and heavy metal ions, can prevent microbial cells from damages in a production process, and have the advantages of effectively extending the shelf life of the preparation and the like. The microbial microcapsule preparation of the present invention can prepare different microbial microcapsule preparations according to different microbial species, and can effectively control capsule wall thickness and diameter of the microcapsules in the preparation process. The microcapsules of the invention have an embedding rate of more than 90% for microbial cells, and can realize industrial mass production.

The invention relates to a method for preparing a high molecular polymer Poly gamma-glumatic acid gamma-PGA from a corn raw material by the liquid state fermentation of bacillus subtilis. The method is characterized in that: the method for preparing a high molecular polymer Poly gamma-glumatic acid gamma-PGA from corns taken as a basic raw material by the liquid state fermentation of the bacillus subtilis screens Bacillus subtilis HCUL-B115, which is stored in the China Center Of Culture Collection, and the storage number is CGMCC No. 2283. The bacillus subtilis HCUL-B115 uses corn, corn starch, corn dextrin, and corn saccharification hydrolysis solution as basic raw materials to prepare the gamma-PGA by the deep liquid state fermentation. The method has the advantages that: the strain (Bacillus subtillis HCUL-B115) can use the corn, the corn starch, the corn dextrine and the corn saccharification hydrolysis solution as the basic raw materials to prepare the gamma-PGA by the liquid state fermentation, and the raw materials are low in price, so that the cost is lowered, and the industrialized production is easily realized.

The invention provides a Bacillus licheniformis engineered bacterium capable of high production of poly-Gamma-glutamic acid, collected under CCTCC No: M2016439 and named as Bacillus licheniformis (WX-02) / pHY-zwf, and further provides a construction method of the Bacillus licheniformis (WX-02) / pHY-zwf and a method for high production of poly-Gamma-glutamic acid. The synthetic level of intracellular NADPH is increased through genetic engineering modification, and the yield of Gamma-PGA. By constructing glucose-6-phosphate dehydrogenase overexpression support pHY-zwf and converting to Gamma-PGA-producing Bacillus licheniformis WX-02 pre-stored in a lab, the Bacillus licheniformis (WX-02) / pHY-zwf capable of producing Gamma-PGA is re-constructed. The bacterium may provide significantly synthesis of Gamma-PGA under optimized media and culture conditions. The yield of Gamma-PGA is up to 51.13 g / L which is 49% significantly higher than the Gamma-PGA yield of an original bacterium.

The invention discloses a preparation method of a biological organic fertilizer having a function of prevention and control of soil-borne plant diseases; the preparation method includes a composting fermentation step; a composting raw material is inoculated with a mixed bacterial liquid of four antagonistic bacteria of bacillus licheniformis, bacillus subtilis, ureibacillus thermosphaericus and bacillus thuringiensis; after composting fermentation is finished, gamma-polyglutamic acid is added in the fermentation product. The prepared biological organic fertilizer has a significant prevention and control effect on plant soil-borne fungal diseases. The production technology of the product is simple, wide in raw material sources, low in production cost, and suitable for mass production of the biological organic fertilizer having the function of prevention and control of the soil-borne plant diseases.

The invention provides a soil conditioner for repairing polluted soil. The soil conditioner is prepared by mixing the following raw materials in percentage by weight: 1-2.5% of a compound microorganism agent, 60-75% of an organic matter, 25-45% of a medium trace element soil conditioner and 5-20% of a macromolecular organic compound, wherein the macromolecular organic compound consists of poly gamma-glutamic acid and humic acid; and humic acid comes from high-quality lignite. The soil conditioner provided by the invention has the effects of improving soil, degrading soil pesticides and residual fertilizers, passivating and fixing farmland heavy metals, reducing adsorption of plants to heavy metals and improving the crop yield and quality, is used for repairing and controlling farmland pollution, and has such remarkable functions of fertilizer supply, soil improvement, soil-ripening progress acceleration, and crop yield and quality improvement.

The present invention relates to a surface expression vector of SARS coronavirus antigen containing a gene encoding an antigen of SARS inducing coronavirus and any one or two or more of genes pgsB, pgsC and pgsA encoding poly-gamma-glutamic acid synthase complex, a microorganism transformed by the surface expression vector, and a SARS vaccine comprising the microorganism. According to the present invention, it is possible to economically produce a vaccine for prevention and treatment of SARS using a recombinant strain expressing an SARS coronavirus antigen on their surface.

The invention discloses a fast preparation method of a nanometer particle gel medicine carrying system. Poly gamma-glutamic acid, Chitosan oligosaccharide, hyaluronic acid, nanometer particles, rhamnolipid and crosslinking agents EDC / NHS are used for preparing gel; under the catalytic effect of crosslinking agents, intermolecular crosslinking reaction is performed. According to the method provided by the invention, the hyaluronic acid is used as a catalyst to be matched with the rhamnolipid and the crosslinking agents EDC / NHS; the gel formation time can be effectively reduced, so that the gel has more excellent gel performance; high bioactivity performance and good biocompatibility are realized; the swelling properties are good; the raw materials are cheap; the obtaining is easy; the production process is simple.

The invention relates to an efficient high-concentration sulfur-based compound fertilizer containing poly-gamma-glutamic acid, which is prepared from urea, phosphoric acid, potassium chloride, potassium sulfate, sulfuric acid, gas ammonia and poly-gamma-glutamic acid, and also comprises a water-retaining agent. A production process of the efficient high-concentration sulfur-based compound fertilizer comprises the steps of: A, preparing a mixed acid; B, neutralizing; C, pelleting and forming; D, preparing monoammonium phosphate; E, supplementing potassium; and F, supplementing nitrogen. The invention has the advantage of being capable of manufacturing the efficient high-concentration sulfur-based compound fertilizer containing the poly-gamma-glutamic acid, with the proportion of N:P:K of 17:17:17. Meanwhile, a poly-gamma-glutamic acid fertilizer synergist is added in the production process and used as a fertilizer absorbing promoting agent used on single nitrogen or phosphorus or potassium or the combination of the nitrogen and the phosphorus as well as the potassium. In addition, the water-remaining agent is added for increasing the water-retaining effect.

The invention provides a macro-element water-soluble fertilizer containing poly-gamma-glutamic acid. The fertilizer comprises the following ingredients in parts by weight: 10-30 parts of nitrogen fertilizer, 10-20 parts of phosphorus fertilizer, 10-30 parts of potassium fertilizer and 0.1-0.5 part of poly-gamma-glutamic acid, that is, the weight ratio of nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer and poly-gamma-glutamic acid is (10-30):(10-20):(10-30):(0.1-0.5). Under the equal cultivation conditions, the crop yield can be increased by 10-30% when the coated fertilizer is applied, and the fertilizer consumption can be reduced by 10-20%; the fertilizer is used for experimental cultivation of wheat, apples, citruses and the like for several years, and the experimental results show that the fertilizer use cost can be effectively reduced by 30% when poly-glutamic acid is added into the fertilizer, and the fertilizer consumption can be reduced by 20-40%; moreover, the fertilizer provided by the invention is poly-element composite fertilizer which can be completely dissolved in water; due to rapid dissolution in water, the fertilizer can be absorbed by crops more easily, and the fertilizer absorption and utilization rate is relatively high; more importantly, the fertilizer can be applied to facility agriculture such as spray irrigation and drop irrigation, the water-fertilizer integration is realized, and the efficacies on water, fertilizer and labor conservation are achieved.

This invention belongs to preparative technique field of Biological Pesticide, Specifically relating to a gamma-PGA which is used as protective additive for Bacillus thuringiensis praeparatum and the its application that it is used as protective agent in thuringiensis praeparatum preparation . using gamma-PGA as Bt praeparatum, can reduce activity loss of Bt praeparatum in spray drying process, compared with control group, the anthelminthic activity, crystalline and gemma content of invented praeparatum raise about 18.55%,11.24% and 20.38% respectively. Besides, the application of this invention elevates anti- ultraviolet irradiation capability of Bt praeparatum in practically using process, after Sunlight shines 7 days, its anthelminthic activity is 4.30 times of control group. The application of this invention elevates sticky and attaching capability of Bt praeparatum at plant strain. It bears rain water underscouring, its anthelminthic activity can achieve more than 2.23 times of control group, accordingly extend residual period.

The invention belongs to the field of sewage treatment, and particularly relates to a composite gel ball containing graphene oxide quantum dots / magnetic chitosan oligosaccharide, and a preparation method and application of the composite gel ball. The preparation method of the composite gel ball comprises the following steps: 1) adding poly-gamma glutamic acid and chitosan oligosaccharide into deionized water until the poly-gamma glutamic acid and the chitosan oligosaccharide are completely dissolved, carrying out cooling, adding a ferroferric oxide dispersion liquid, and carrying out stirringto prepare a magnetic chitosan oligosaccharide solution; 2) adding polyvinyl alcohol and sodium alginate into deionized water to completely dissolving the polyvinyl alcohol and the sodium alginate, carrying out cooling to obtain a polyvinyl alcohol / sodium alginate water solution, and uniformly mixing the polyvinyl alcohol / sodium alginate water solution with the magnetic chitosan oligosaccharide solution to obtain a composite embedded carrier water solution; and 3) uniformly mixing graphene oxide quantum dots with the composite embedded carrier water solution to obtain a mixed solution, dropwise adding the mixed solution into a calcium chloride / saturated boric acid solution until small balls are formed, carrying out fixing and cross-linking, and carrying out low-temperature preservation toobtain the composite gel ball. The composite gel ball prepared by the method is large in adsorption capacity and good in mechanical strength and is recyclable.

The invention belongs to the technical field of microbial fermentation and particularly relates to a batch fermentation method for efficient coproduction of poly-gamma-glutamic acid and 2,3-butanediol by virtue of bacillus. According to the method, by liquid-submerged fermentation, two products, namely, poly-gamma-glutamic acid and 2,3-butanediol are simultaneously produced. The process can be used for safely producing the strain, the production cost is effectively reduced, the highest concentrations of simultaneously produced poly-gamma-glutamic acid and 2,3-butanediol can respectively reach 52.2g / L and 73.68g / L, the apparent conversion rate of glucose reaches 83.9%, the production strength reaches 2.10g.L<-1>.h<-1>, and the method is suitable for industrial production.

The invention discloses a nanometer platinum polymer, and a preparation method and application thereof. The nanometer platinum polymer is prepared from the following raw materials of nanometer platinum, chitosan oligosaccharide, poly gamma-glutamic acid, propanediol and cross linking agents EDC / NHS. The nanometer platinum polymer obtained through preparation can be applied to medical products, in particular to the wound dressing aspect. Compared with the prior art, the obtained nanometer platinum polymer medicine has good biocompatibility and antibacterial activity; the nanometer platinum is preferably selected to be used as the medicine; propanediol is used as a lubricating agent; the medicine coating rate is higher; high degradability is realized; the swelling behavior performance is good; the raw materials are cheap and can be easily obtained; the production process is simple.

The invention relates to a filler-free blended fertilizer consisting of urea, monoammonium phosphate, potassium chloride, ammonium chloride and poly-gamma-glutamic acid, wherein the filler-free blended fertilizer has the formulation based on parts by weight: 3-41 parts of urea with 46% of N, 12-60 parts ammonium chloride with 20-25% of N, 25-45 parts of monoammonium phosphate with 45-55% of P2O5, 23-28 parts of potassium chloride with 60% of K2O, and 0.1-1 part of poly-gamma-glutamic acid. The blended fertilizer has the advantages that the blended fertilizer is free of filler, thus being attractive in appearance, and good in water solubility. The filler-free blended fertilizer is scientific and reasonable in proportion, but the production cost is only 1949 RMB / ton, so that the cost is saved about 441 RMB / ton compared with the cost of the original filler-containing blended fertilizer. The filler-free blended fertilizer is simple in technology, so that the filler-free blended fertilizer can be prepared by mixing raw materials according to the formulation proportion.

The invention relates to the field of biotechnology and fermentation and discloses a preparation method of bacillus licheniformis for producing poly gamma-glutamic acid at high yield and application.The preparation method is characterized in that on the basis of plasmid T2(2)-Ori, a cimH gene is successfully knocked out from bacillus licheniformis WX-02 by constructing a knockout vector T2-deltacimH of citric acid transporter gene cimH, and further engineered bacterium WX-02 deltacimH of the bacillus licheniformis is obtained. Compared with contrast bacterium WX-02, an engineered strain WX-02deltacimH has the advantages that the yield of gamma-PGA is at least improved by 11 percent or above, and a new strategy is provided for high yield of the gamma-PGA.

The invention discloses water soluble fertilizer with poly-gamma-glutamic acid and application thereof. Compared with existing water soluble fertilizer, the adopted poly-gamma-glutamic acid is anionic polyamino acid produced through microbial fermentation, has rich side chain carboxyl, is nontoxic and harmless, has no residue, can chelate various mineral ions, and has the good effect on improving the soil structure, improving the soil moisture and fertilizer retention capacity, promoting crop root system ion exchange. Experiments prove that on the condition of ensuring normal growth of muskmelon, 300 ml of water soluble fertilizer with poly-gamma-glutamic acid is applied in each mu of field, the chemical fertilizer application amount can be reduced by 20-30%, and the cost is reduced; on the basis of ensuring the normal chemical fertilizer application amount of muskmelon, 300 ml of water soluble fertilizer with poly-gamma-glutamic acid is applied in each mu of field, so that the muskmelon yield is improved by 40-42%, and benefits are improved; in addition, the muskmelon quality is improved, and the muskmelon is more sweet, crisper and more delicious in taste.

A recombinant microorganism having a poly-gamma-glutamic acid-producing ability, in which among a gene cluster associated with poly-gamma-glutamic acid synthesis, pgsB gene of Bacillus subtilis or a gene corresponding to the gene and pgsC gene of Bacillus subtilis or a gene corresponding to the gene have been introduced into a host microorganism, and pgsA gene of Bacillus subtilis or a gene corresponding to the gene has not been introduced into the host microorganism; and a method for producing poly-gamma-glutamic acid using the recombinant microorganism.

The invention relates to a microorganism producing poly gamma-glutamic acid and a construction method and application thereof. The microorganism producing poly gamma-glutamic acid disclosed by the invention is a microorganism capable of overexpressing phospholipase C, and the overexpression is introducing one or a plurality of copied phospholipase C encoding genes and / or replacing the expression element of phospholipase C with an expression element with the relatively high activity. The microorganism is obtained by improving expression of phospholipase C, and the yield of poly gamma-glutamic acid produced through fermentation of the microorganism is obviously improved. The microorganism provides new strategies for improving the yield of poly gamma-glutamic acid produced through fermentation of the microorganism and reducing the production cost.

Methods for producing high molecular weight poly-gamma-glutamic acid (PGA). The PGA is produced by fermentation, and purified by use of tangential flow filtration, followed by diafiltration, as necessary, to yield a product of the desired purity. Product obtained may be of very high purity using all the prescribed purification steps. Product of this purity is suitable for in vivo medical applications. Other applications, such as food or agricultural, may utilize lower purity levels, and hence do not require all the purification steps specified.

The invention relates to a technology of preventing soil salinization, in particular to a method for improving soil salinization. The biodegradation type method for improving saline-alkali soil comprises the steps of: firstly, preparing nutrient-supplying agent mother liquor for conditioning soil: mixing according to the mixture ratio of 50-70 percent of high polymer product PGA (poly-gamma-glutamic acid) subjected to microbial metabolism, 25-35 percent of carbamide and 5-15 percent of root-inducing agent, and dissolving the mixture in water to make the mother liquor according to the proportion of 1:15; secondly, diluting the mother liquor obtained in the first step by 300-500 folds, irrigating the mother liquor on the surface of soil before planting, or adding the diluted mother liquor in water used for watering seedlings during the waking period after plant seedlings are transplanted; and thirdly, irrigating the diluted mother liquor again in a week. According to the method provided by the invention, no adverse effect can be caused on the soil, and the method is adaptable to different areas.

The invention relates to a hydrogel sour pomegranate powder skin care product as well as a preparation method and application thereof. The hydrogel sour pomegranate powder skin care product is prepared from raw materials, namely poly gamma-glutamic acid, chitosan oligosaccharide, hyaluronic acid and a cross-linking agent EDC / NHS, rhamnolipid and sour pomegranate powder through steps of jucing, purification, freeze drying and extraction. The hydrogel sour pomegranate powder skin care product provided by the invention is good in biocompatibility, antibacterial property, efficient moistening property and skin whitening property. As hydrogel is preferably adopted as a skin care product matrix, and the sour pomegranate powder is adopted as an active component, the skin care product is relatively good in water retention property, relatively low in water loss rate, good in skin whitening function and relatively good in biodegradability; in addition the raw materials are cheap and easy to obtain, only a small amount of chemical substance products are used, and the components are mostly degradable macromolecule polymers generated from microorganisms instead, so that the product is environmentally friendly, free of toxic or side effect and simple in production process, and on-batch production can be implemented.

The invention provides a bioorganic fertilizer with a Bok choy anthrax control function. The raw materials of the bioorganic fertilizer comprise Paenibacillus polymyxa, Bacillus subtilis, a compost produced from animal manure and plant residues, and a poly(gamma-glutamic acid) solution. The invention also provides a preparation method of the bioorganic fertilizer. The preparation method comprises the following steps: inoculating he compost produced from the animal manure and the plant residues with a mixed bacterial liquid prepared after culturing and activating two antagonistic bacteria comprising Paenibacillus polymyxa and Bacillus subtilis, carrying out fermentation to obtain a decomposed product, adding the a poly(gamma-glutamic acid) solution, and drying the obtained mixture in a shady place to prepare the bioorganic fertilizer with a Bok choy anthrax control function. The bioorganic fertilizer prepared in the invention has a substantial control effect on Bok choy anthraxes; and the preparation method of the product has the advantages of simplicity, wide sources of the raw materials, and low production cost, and is suitable for large-scale production of the bioorganic fertilizer with the Bok choy anthrax control function.