Biological organic fertilizer having function of prevention and control of soil-borne plant diseases and preparation method thereof

A bio-organic fertilizer and plant disease technology, applied in the field of agricultural biology, can solve the problems of increasing prevention and control costs, the environment of chemical residues, and the limitation of crop rotation methods, and achieve the effects of improving product quality, improving the structure of aggregates, and reducing the application of pesticides.

Inactive Publication Date: 2016-01-06
DALIAN UNIV OF TECH
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  • Claims
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Problems solved by technology

The current disease prevention and control mainly relies on chemical control, but long-term application of chemical agents will also cause problems such as chemical residues and environmental pollution; in conventional agricultural management, plant pathogens can exist in the soil for several years, and the crop rotation method when the disease breaks out Greatly limited, grafting and other methods have greatly increased the cost of prevention and control

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  • Biological organic fertilizer having function of prevention and control of soil-borne plant diseases and preparation method thereof

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preparation example Construction

[0017] In the preparation method of the above bio-organic fertilizer, the inoculation ratio of the mixed bacterial solution in the compost raw material is 0.01~0.05L·kg -1 (The volume of the mixed bacterial solution inoculated in every kilogram of compost raw materials). Among them, 0.03L·kg is especially preferred -1 .

[0018] In a preferred specific embodiment, the preparation method of the bio-organic fertilizer also includes adding 0.08 to 0.12 L·kg to the compost fermentation product -1 The step of adding the mixed bacterial solution in the ratio.

[0019] In the preparation method of the above-mentioned bio-organic fertilizer, the poly-γ-glutamic acid is preferably pre-prepared with distilled water as 200mg L -1 Poly-γ-glutamic acid solution, the amount added is 0.03-0.05L·kg -1 (Volume of poly-gamma-glutamic acid-distilled aqueous solution added per kilogram of fermentation product).

[0020] In another specific embodiment, the preferred composting raw material of...

Embodiment 1

[0035] Prepare the bio-organic fertilizer I with the function of preventing and controlling soil-borne plant diseases, comprising the steps of:

[0036] 1. Preparation for stockpiling

[0037] After crushing animal manure such as pig manure, chicken manure or cow manure and plant residues such as hay, branches or straw, pass through a 1-mesh screen, and then mix according to the mass ratio of animal manure: plant residue 4:1 to adjust the water content of compost raw materials The rate is 60%, save it for later use. The water contents of the selected animal manure and plant residues are 75% and 5% (percentage by mass) respectively.

[0038] 2. Preparation of mixed bacterial solution and poly-γ-glutamic acid solution:

[0039] The antagonistic bacteria Bacillus licheniformis ABTNL-1, Bacillus subtilis ABTNL-2, Bacillus thermospheroidus ABTNL-3 and Bacillus thuringiensis ABTNL-4 were cultivated in liquid medium until the bacterial concentration in each antagonistic bacteria li...

Embodiment 2

[0046] The preparation of bio-organic fertilizer II with the function of preventing and controlling soil-borne plant diseases comprises the following steps:

[0047] 1. Preparation for stockpiling

[0048] With embodiment 1 step 1.

[0049] 2. Preparation of mixed bacterial solution and poly-γ-glutamic acid solution:

[0050] The antagonistic bacteria Bacillus licheniformis ABTNL-1, Bacillus subtilis ABTNL-2, Bacillus thermospheroidus ABTNL-3 and Bacillus thuringiensis ABTNL-4 were cultivated in liquid medium until the bacterial concentration in each antagonistic bacteria liquid was equal to for 10 8 ~10 9 CFU·ml -1 , the four cultures were mixed in equal volume proportions to obtain a mixed bacterial solution; the liquid medium was prepared by dissolving 10.0 g of sodium chloride, 10.0 g of tryptone, and 5.0 g of yeast powder in 1L of distilled water, and sterilized at 121°C for 20 minutes. Use after sterilization.

[0051] In addition, distilled water was used as a sol...

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Abstract

The invention discloses a preparation method of a biological organic fertilizer having a function of prevention and control of soil-borne plant diseases; the preparation method includes a composting fermentation step; a composting raw material is inoculated with a mixed bacterial liquid of four antagonistic bacteria of bacillus licheniformis, bacillus subtilis, ureibacillus thermosphaericus and bacillus thuringiensis; after composting fermentation is finished, gamma-polyglutamic acid is added in the fermentation product. The prepared biological organic fertilizer has a significant prevention and control effect on plant soil-borne fungal diseases. The production technology of the product is simple, wide in raw material sources, low in production cost, and suitable for mass production of the biological organic fertilizer having the function of prevention and control of the soil-borne plant diseases.

Description

technical field [0001] The invention relates to a bio-organic fertilizer capable of preventing and controlling soil-borne plant diseases and a preparation method thereof, belonging to the field of agricultural biotechnology. Background technique [0002] With the separation of aquaculture and planting in our country, there is not enough farmland to absorb a large amount of aquaculture waste produced by large-scale and intensive aquaculture, which has a serious impact on the ecological environment. However, the application of chemical fertilizers in farmland is increasing year by year, and the bottleneck of contribution to crop yield is becoming increasingly prominent. [0003] Fertilization in my country's agricultural production is characterized by partial application of chemical fertilizers. The long-term application of large amounts of chemical fertilizers reduces the quality of crops, causes soil compaction, decline in organic matter, damage to the micro-ecological struc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C05G3/80
Inventor 徐永平王佳宁续彦龙龚改林王丽丽李晓宇
Owner DALIAN UNIV OF TECH
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