Poly-gamma-glutamic acid generation bacteria and method for producing poly-gamma-glutamic acid

A glutamic acid and strain technology, applied in the field of applied microorganisms, can solve the problems of high fermentation cost, long fermentation period, limited performance application range, etc., and achieve the effects of stable genetic traits, reduced production costs, and fast growth speed.
CN1536071AInactive Publication Date: 2004-10-13HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2004-10-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a strain capable of producing poly-gamma-glutamic acid and a method for producing poly-gamma-glutamic acid by utilizing said strain. The described strain is X-003, a Bacillussubilis, CCTCC, NO:M202048. It adopts seed liquor preparation and liquid submerged fermentation process to produce and obtain the poly-gamma-glutamic acid product. The hereditary feature of said strain is stable, and its capacity for synthesizing poly-gamma-glutamic acid is strong, and the molecular weight of synthetic poly-gamma-glutamic acid can be up to above 10000 KD. The shake-flask fermentation level of poly-gamma-glutamic acid by utilizing said invented strain and its fermentation method can be up to 10 mg / ml, and the fermentation level of the fermentation tank with 80L is 30 mg / ml.
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Description

technical field

[0001] The invention belongs to the technical field of applied microorganisms, and in particular relates to a poly-gamma-glutamic acid producing bacterium selected and a method for producing poly-gamma-glutamic acid using the strain. Background technique

[0002] Poly-γ-glutamic acid (poly-γ-glutamic acid) is a biodegradable polymer composed of D-type and L-type glutamic acid linked by peptide bonds, with an average molecular weight ranging from 10,000 to 2,000,000 Daltons (Matsukawa et al.1997 Hydrophilic surface treating aqueous solution and hydrophilic surfacetreating method, U.S.Patents 5.616,585.Ito, Y., Tamaka et al., 1996.Glutamic acid Independent Production of poly(glutamic acid) by Bacillus 4nol-subtilisBiotech. .Biochen.60:1239-1242; Cromwick et al., Effect of magnese (II) on Bacilluslicheniformis ATCC9945A physiology and poly-(glutamic acid)formation.Int.J.Biol.Macromol.17:259-267; Cromwick et al., 1995 b .Investigati...

Claims

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