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Microcapsule preparation, preparation method and application

A technology of microcapsules and preparations, applied in the field of agricultural microorganisms, can solve the problems of high requirements for microcapsule equipment, unsuitable for large-scale production, weak adaptability to the environment, etc. The effect of easy transportation and preservation

Inactive Publication Date: 2012-11-07
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] In a large number of previous studies, microcapsule technology has achieved certain development, but there are still some unresolved problems, such as low embedding rate, poor slow-release performance, high requirements for equipment for preparing microcapsules or raw materials. Not easy to obtain and not suitable for large-scale production, etc.
[0011] At present, microorganisms, as the main body of microbial fertilizers and microbial pesticides, are widely used in agricultural production, but microbial products have the disadvantages of poor stress resistance, weak ability to adapt to the environment, and strong variability.
In recent years, people have been researching on enhancing the microbial activity of microbial products in the process of production, transportation, storage and application, and achieved certain results, but they still cannot meet the requirements of higher standards.

Method used

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  • Microcapsule preparation, preparation method and application
  • Microcapsule preparation, preparation method and application
  • Microcapsule preparation, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] 1. The isolation and screening process of Bacillus subtilis 3-10;

[0065] One of the microorganisms used in the capsule core of the microcapsule preparation of the present invention is a biocontrol bacterium Bacillus subtilis which is obtained by separating and screening from the vegetable garden soil of the Huazhong Agricultural University campus in the Shizishan District, Wuchang, Wuhan, Hubei Province. Bacillus subtilis 3-10, which was sent to the China Center for Type Culture Collection (CCTCC) in Wuhan University, Wuhan, Hubei Province on March 30, 2011, and its preservation number is CCTCC NO: M2011100.

[0066] 2. Isolation, identification and mycological characteristics of Bacillus subtilis 3-10

[0067] (1) Isolation and identification of Bacillus subtilis 3-10

[0068] A biocontrol bacterium Bacillus subtilis (Bacillus subtilis) 3-10 suitable for the prevention and treatment of watermelon wilt of the present invention was isolated and screened on the vegetab...

Embodiment 2

[0098] According to the method described in Example 1, in this example, the microorganism used for the capsule core is Bacillus mucilaginosus, preservation number: ACCC NO: 10168, purchased from China Agricultural Microorganism Culture Collection Center ( Address: No. 12, Zhongguancun South Street, Haidian District, Beijing), its spores and bacteria are obtained through fermentation.

[0099] The fermentation medium formula and conditions are as follows:

[0100] Medium: soybean meal 42g / L, cornstarch 8g / L, CaCO 3 1g / L, K 2 HPO 4 0.25g / L, FeCl 3 0.015g / L, magnesium sulfate heptahydrate 2.4g / L; Supplement distilled water to 1000ml; pH 7.0-7.2; Sterilize according to conventional methods (121°C high-pressure steam sterilization for 20min).

[0101] Fermentation conditions: shake flask 100ml / 500ml liquid volume, 28°C, shaker 180r / min for 24 hours.

[0102] Wherein the concentration of poly-γ-glutamic acid in step (1) and gelatin solution is all 1.5%, the consumption of gel...

Embodiment 3

[0107] According to the method described in Example 1, in this example, the microorganism used for the cystic core is Bacillus megaterium (Bacillus megaterium), preservation number: ACCC NO: 10010, purchased from China Agricultural Microorganism Culture Collection Center (address: No. 12, Zhongguancun South Street, Haidian District, Beijing), and its spores and thallus are obtained by fermentation.

[0108] The fermentation medium formula and conditions are as follows:

[0109] Medium: cornstarch 20.0g, peptone 15.0g, soybean meal 30.0g, KH 2 PO 4 2.0g, calcium carbonate 2.0g, magnesium sulfate 1.0g, manganese sulfate 0.5g; add distilled water to 1000ml; pH pH7.5; sterilize according to conventional methods (121°C autoclaving for 20min).

[0110] Fermentation conditions: shake flask 100ml / 500ml liquid volume, 28°C, shaker 180r / min for 36 hours.

[0111] Wherein the concentration of the poly-γ-glutamic acid in the step (1) and the gelatin solution is 2.5%, the consumption of...

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Abstract

The present invention belongs to the technical field of agricultural microbe, and specifically relates to a microbial capsule preparation taking poly-gamma-glutamic acid as a wall material, a preparation method thereof, and applications thereof in preparing microbial pesticides and microbial fertilizers. The microbial microcapsules taking the poly-gamma-glutamic acid as the wall material have good mechanical strength and flexibility, can be applied in microbial embedding medium for good sustained-release ability, can prevent microbial cells from injuries of ultraviolet rays, temperature, acid or base, ionic strength and heavy metal ions, can prevent microbial cells from damages in a production process, and have the advantages of effectively extending the shelf life of the preparation and the like. The microbial microcapsule preparation of the present invention can prepare different microbial microcapsule preparations according to different microbial species, and can effectively control capsule wall thickness and diameter of the microcapsules in the preparation process. The microcapsules of the invention have an embedding rate of more than 90% for microbial cells, and can realize industrial mass production.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a microbial capsule preparation using poly-γ-glutamic acid as a wall material, its preparation method and its application in the preparation of microbial pesticides and microbial fertilizers. Background technique [0002] Microcapsule technology uses natural or synthetic polymer materials as the capsule wall, and wraps one or more active substances by chemical, physical or physicochemical methods to form tiny particles with semi-permeable or sealed capsules. . The encapsulation process is called microencapsulation, and the tiny particles formed are called microcapsules (Zhang Jun et al., 2005). The advantage of microencapsulation is that when microcapsules are formed, the capsule core is covered and isolated from the external environment, and its properties can be preserved without any influence. Under appropriate conditions, the capsule core can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N63/02A01N25/28A01P3/00C05G3/00
Inventor 陈守文冀志霞邓有辉马昕舒芹祁高富姚德惠陈建刚
Owner HUAZHONG AGRI UNIV
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