Use of poly-gamma-glutamic acid as fertilizer absorbefacient in agricultural planting

An absorption accelerator, glutamic acid technology, applied in the application, agriculture, fertilizer mixture and other directions, to achieve the effect of improving quality, expanding the scope of application, and increasing the value of environmental protection

Inactive Publication Date: 2006-09-06
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to expand the application range of poly-γ-glutamic acid. As a biodegradable fertilizer absorption accelera...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Production of poly-γ-glutamic acid by solid-state fermentation

[0022] Poly-γ-glutamic acid can also be produced by solid-state fermentation. In the present invention, pig manure or cow dung is used as the main raw material, and auxiliary materials such as soybean meal, bran, glutamic acid and citric acid are added in a certain proportion. Bacillus subtilis CCTCC202048 was inoculated, and the bio-organic fertilizer containing poly-γ-glutamic acid was produced by outdoor accumulation and fermentation.

[0023] The solid fermentation method is as follows:

[0024] 1) Preparation of seed liquid: activate the strain, and then inoculate the activated strain in the seed liquid medium. The components of the medium and their proportions are as follows: beef extract 10.0g, peptone 5.0g, NaCl 10.0g, Distilled water 1000ml, pH7.2-7.4. The cultivation conditions and methods of the seeds are as follows: cultivate at 30°C for 4.5h, and take the seeds grown to the mid-lo...

Embodiment 2

[0032] Embodiment 2: poly-γ-glutamic acid (average molecular weight 200 kilodaltons) to the nutrient accumulation of Chinese cabbage stems and leaves cultivated in nutrient pots

[0033]Experimental procedure: choose plump and consistent pakchoi seeds (the variety is Shanghai Qing) to germinate. After germination 3d, after diluting the standard nutrient solution (see the third article of the content of the invention) by 4 times, pouring and cultivating; after 7 days, diluting the standard nutrient solution by 2 times, pouring and cultivating; The consistent seedlings were transplanted into hydroponic black plastic pots (7cm×15cm), and divided into 4 groups. One group added 400mL standard nutrient solution to each pot, and the nutrients in the rest of the groups were diluted by 2 times and 4 times the standard nutrient solution. times and 8 times, each large group was further divided into 5 groups, and poly-γ-glutamic acid was added respectively, and the final concentration of ...

Embodiment 3

[0039] Embodiment 3: the influence of poly-γ-glutamic acid root irrigation (average molecular weight 10000 kilodaltons) on the yield of potted Chinese cabbage

[0040] Test treatment: without adding poly-γ-glutamic acid and adding different concentrations of poly-γ-glutamic acid (25mg / kg soil, 50mg / kg soil, 100mg / kg soil, 200mg / kg soil, 400mg / kg soil ), a total of 6 groups, the amount of nutrient element application of the base fertilizer was established in 4 groups, one group was 0.20g of nitrogen, 0.065g of phosphorus, and 0.16g of potassium applied to 1kg of soil, and the types of fertilizers were commercially available commercial fertilizers: urea, superphosphate, chlorine Potassium, the rest of the nutrients in each group were reduced to the original 75%, 50%, 33%.

[0041] Experimental procedure: dissolve fertilizer in water and mix with poly-γ-glutamic acid (average molecular weight 1000 kilodalton) solution, weigh 1 kg of soil in each pot, mix it with the above fertili...

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Abstract

The invention relates to the application of the gamma-polyglutamic acid as the absorption promoting agent of the fertilizer in the agricultural husbandry. The said gamma-polyglutamic acid is a high molecular polymer of D-glutacid and L- glutacid formed by the alpha-amino and the gamma-carboxyl in the manner of peptide bond and there is also a dissociative carboxyl on the alpha-carbon atom of every repetitive unit with average molecular weight of 10-10,000 kilodaltons. The said gamma-polyglutamic acid can be used as the absorption promoting agent of the fertilizer made of single element of nitrogen, phosphor, kalium or their composition. The significance of the invention is: it can be degraded by edaphon and its environmental value is much better in relation to TPA; it can reduce by 10-30 % of the normal usage of the chemical fertilizer by applying 10-50 grams of fertilizer per area and increase by 10-30 % of the crop yield, at the same time it can improve the crop quality and increase the crop content of nitrogen, phosphor and kalium.

Description

technical field [0001] The invention relates to the application of poly-γ-glutamic acid as a fertilizer absorption accelerator in agricultural planting, and belongs to the field of microbial fertilizer application. Background technique [0002] Chemical fertilizer is a major change in the history of agricultural development. It has greatly increased the yield of crops and eased the global food shortage. my country's chemical fertilizer consumption reaches more than 40 million tons per year, accounting for 30% of the world's total consumption of chemical fertilizers, but the seasonal utilization rate of nitrogen is 30%-35%, phosphorus is 10%-25%, and potassium is 35%-50%. According to statistics, more than 60 of the more than 130 large and medium-sized lakes in my country have encountered eutrophication problems. The root cause is the migration of excess nitrogen and phosphorus. Therefore, today's fertilizer nutrition problem is no longer the...

Claims

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Application Information

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IPC IPC(8): C05G3/00
Inventor 陈守文喻子牛陈雄蔡皓柯云孙明何进
Owner HUAZHONG AGRI UNIV
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