10 results about "Amino acid fermentation" patented technology

The application discloses a kind of process methods for optimizing control production 5-aminovaleric acid, step one, amino acid fermentation seed is inoculated in culture medium, and aerobic fermentation culture is carried out;During fermentation culture process, fermentation temperature is controlled at 30-35 DEG C, pH value is maintained at 6.5-7.5, and by controlling aeration and stirring, dissolved oxygen level is controlled at 20%-30%;After fermentation, fermentation broth is separated, and 5-aminovaleric acid crude product is obtained;5-aminovaleric acid crude product is sequentially subjected to decolorization, crystallization and refining, and finally 5-aminovaleric acid finished product is obtained.The application uses optimized fermentation conditions, by accurately controlling fermentation temperature, pH value and dissolved oxygen level, to ensure the stability and efficiency of fermentation process, improve product quality and yield.

This invention relates to the field of amino acid mother liquor resource utilization technology, specifically disclosing a method for the resource utilization of amino acid mother liquor. Specifically, the method involves: treating the amino acid mother liquor by electrodialysis to obtain an inorganic salt solution and desalted material; using the inorganic salt solution as an inorganic salt supplement for reuse in the amino acid fermentation production process; concentrating, crystallizing, and separating the desalted material to obtain crude amino acids and final mother liquor; and spray-granulating the final mother liquor to produce organic fertilizer. Compared with existing ion exchange and chromatographic separation technologies, the process provided by this invention has significant advantages such as simple flow, stable operation, no need for acid-base regeneration, and low equipment investment and operating costs. It achieves both amino acid resource recovery and the resource utilization of inorganic salt impurities, realizing a balance between clean production and economic benefits.

This invention relates to the field of feed, specifically disclosing a method for preparing selenium- and strontium-enriched amino acid chelated feed; including the preparation of selenium / strontium-enriched amino acid fermentation products, the preparation steps of which are as follows: S1: Inoculating activated microalgae and yeast strains into a culture medium supplemented with selenium and strontium compounds, the pH of the culture medium is 6.5-7.5, the culture temperature is 25-35℃, and the culture is carried out under light and aeration conditions for 5-7 days; S2: Collecting selenium / strontium-enriched algal biomass using a flocculant, and ultrasonically treating the selenium / strontium-enriched algal biomass for 10-20 minutes, then adding soybean meal and rapeseed cake to the selenium / strontium-enriched algal biomass and stirring; S3: Adding cellulase, papain, alkaline protease, and pectinase for enzymatic hydrolysis to obtain selenium / strontium-enriched amino acid fermentation products. The selenium- and strontium-enriched amino acid chelated feed and its preparation method of this invention can improve the intestinal health of animals, increase the absorption and utilization rate of nutrients, and enhance animal immunity.

The application provides a fermentation tank feeding device for amino acid production and a use method, and belongs to the technical field of amino acid fermentation production equipment, and comprises a knocking mechanism, the knocking mechanism comprises a fermentation tank body, a lifting device is arranged on one side of the fermentation tank body, a hopper is arranged on the lifting device, a discharging plate is arranged at the lower part of the hopper, a lower frame is arranged outside the discharging plate, a vibrating hammer is arranged on the lower frame, a transmission assembly is arranged on one side of the vibrating hammer, and the transmission assembly is used for driving the vibrating hammer to knock off the wall-hanging material on the inner wall of the hopper; a frequency adjusting mechanism comprises an upper frame fixedly arranged on the upper part of the hopper, and a lifting mechanism is arranged at the lower part of the upper frame, and the lifting mechanism is used for changing the knocking frequency of the vibrating hammer on the hopper according to the difference between dry and wet materials. The application solves the problems of material waste caused by wall-hanging of materials and feeding caused by different wall-hanging characteristics of dry and wet materials.

This invention discloses a strain that produces high levels of L-homoserine, its construction method, and its applications, belonging to the field of amino acid fermentation. This invention provides a strain for efficient fermentation production of L-homoserine, its construction method, and its applications. This strain utilizes a cofactor homogenization scheme to introduce a heterologous NADH-dependent dehydrogenase to replace the original NADPH-dependent dehydrogenase in *E. coli*, thereby removing cofactor limitations, knocking out the main byproduct pathway, enhancing precursor supply, and strengthening efflux protein expression. Fed-batch fermentation in a fermenter achieves a yield as high as 155 g / L.

The present application relates to the technical field of genetic engineering, in particular to transcriptional regulator PcaR, mutant thereof and engineering bacteria thereof. The present application finds that the transcriptional regulator PcaR has the function of regulating the production of amino acids such as threonine, and can be used as a metabolic modification target point of amino acid production strains. The present application provides a mutant of the transcriptional regulator PcaR, which can significantly improve the production capacity of bacteria for amino acids such as threonine. The threonine yield and conversion rate of the engineering bacteria constructed based on the mutant are significantly improved, and the engineering bacteria have good application prospects in the field of amino acid fermentation.

The present application relates to the technical field of wheat bio-organic foliar fertilizer, and discloses a wheat zinc-selenium bio-organic foliar fertilizer and a preparation method thereof, which comprises: 300-400 parts of fermentation substrate, 40-60 parts of zinc sulfate, 2-5 parts of sodium selenite, 15-25 parts of alginic acid extract, 80-120 parts of compound amino acid, 1-2 parts of potassium sorbate and 435-554 parts of water. The present application combines zinc-selenium nutrient fortification with the biological stimulation of alginic acid extract, compound amino acid and fermentation substrate. The alginic acid extract can promote the growth of wheat root system, improve the photosynthetic efficiency of leaves and enhance the drought resistance and disease resistance of wheat. The compound amino acid can be directly absorbed and utilized by wheat, supplement the raw materials for protein synthesis and improve the quality of grains. The organic matter and beneficial bacteria metabolites in the fermentation substrate can regulate the growth rhythm of wheat, promote nutrient transport, and achieve the effect of trinity of wheat nutrient fortification, stress resistance enhancement and yield increase.

This paper discloses the use of modified Escherichia coli and its L-amino acid fermentation production. Specifically, it discloses the use of pyruvate formate lyase, alcohol dehydrogenase, branched-chain amino acid transaminase derived from Escherichia coli, branched-chain amino acid transaminase derived from Bacillus subtilis, thiamine phosphate synthase, and maleate dehydrogenase in the construction of genetically modified bacteria that produce L-amino acids. Experiments have shown that by knocking out the genes encoding pyruvate formate lyase, alcohol dehydrogenase, branched-chain amino acid transaminase derived from Escherichia coli, thiamine phosphate synthase, and maleate dehydrogenase in E. coli capable of producing L-amino acids, and introducing the gene encoding branched-chain amino acid transaminase derived from Bacillus subtilis, recombinant bacteria favorable for L-amino acid accumulation can be obtained. These recombinant bacteria can significantly increase L-amino acid production and reduce costs, which is of great significance in accelerating the industrialization process of L-amino acids.

The utility model relates to amino acid fermentation liquor processing technical field discloses a disc type separator, including separator main part, the upper end of separator main part is fixedly connected with feed pipe and discharge pipe respectively, pretreatment subassembly, pretreatment subassembly sets up between separator main part and feed pipe, pretreatment subassembly is used for the pretreatment of amino acid fermentation liquor, and pretreatment subassembly includes installation shell, mixing pipe and driving motor, and through the setting of separator main part and pretreatment subassembly realizes the coherent realization of the pretreatment before amino acid fermentation liquor processing and the separation step of amino acid fermentation liquor, reduces processing step, improves processing efficiency, the heating wire in installation shell is electrified heating after the device works, and the enzyme in amino acid fermentation liquor is inactivated by heating the amino acid fermentation liquor that flows through the installation shell, prevents the target object inactivation, and the quantitative amino acid fermentation liquor and treating agent are sent into the mixing pipe through the conveying pipe.

This invention belongs to the field of bioengineering technology, specifically relating to a nutritional composition, its preparation method, and its application. By weight, the nutritional composition comprises 80-98 parts yeast extract and 1-15 parts free methionine. It also includes 0.1-6 parts choline chloride, 0.1-2 parts niacin, 0.01-0.15 parts vitamin B2, 0.01-0.2 parts vitamin B6, 0.01-0.25 parts vitamin B5, 0.001-0.05 parts vitamin B1, 0.001-0.06 parts vitamin C, and 0.0001-0.0008 parts biotin. The peptides with a molecular weight of 1000 Da or less account for at least 70% of the total peptides in the nutritional composition. The nutritional composition provided by this invention can be efficiently utilized by the producing strains during the production of amino acid fermentation broth, resulting in fewer impurities in the fermentation broth and facilitating the extraction of fermentation products. Simultaneously, it shortens the fermentation cycle of the amino acid fermentation broth, increases the amino acid concentration, and improves the sugar-acid conversion rate.