18 results about "Amino acid fermentation" patented technology

The application discloses a kind of process methods for optimizing control production 5-aminovaleric acid, step one, amino acid fermentation seed is inoculated in culture medium, and aerobic fermentation culture is carried out;During fermentation culture process, fermentation temperature is controlled at 30-35 DEG C, pH value is maintained at 6.5-7.5, and by controlling aeration and stirring, dissolved oxygen level is controlled at 20%-30%;After fermentation, fermentation broth is separated, and 5-aminovaleric acid crude product is obtained;5-aminovaleric acid crude product is sequentially subjected to decolorization, crystallization and refining, and finally 5-aminovaleric acid finished product is obtained.The application uses optimized fermentation conditions, by accurately controlling fermentation temperature, pH value and dissolved oxygen level, to ensure the stability and efficiency of fermentation process, improve product quality and yield.

This invention relates to the field of amino acid mother liquor resource utilization technology, specifically disclosing a method for the resource utilization of amino acid mother liquor. Specifically, the method involves: treating the amino acid mother liquor by electrodialysis to obtain an inorganic salt solution and desalted material; using the inorganic salt solution as an inorganic salt supplement for reuse in the amino acid fermentation production process; concentrating, crystallizing, and separating the desalted material to obtain crude amino acids and final mother liquor; and spray-granulating the final mother liquor to produce organic fertilizer. Compared with existing ion exchange and chromatographic separation technologies, the process provided by this invention has significant advantages such as simple flow, stable operation, no need for acid-base regeneration, and low equipment investment and operating costs. It achieves both amino acid resource recovery and the resource utilization of inorganic salt impurities, realizing a balance between clean production and economic benefits.

The invention discloses a device and a method for realizing stable acid production of an amino acid fermentation kettle by accurately controlling a heat exchange temperature difference, and relates to the technical field of bioengineering. The device comprises a water mixer, a first refrigerant water inlet and a first refrigerant water outlet are formed in the top and the bottom of the water mixer respectively, a second refrigerant water inlet and a second refrigerant water outlet are formed in the two sides of the water mixer respectively, the first refrigerant water inlet is communicated with a refrigerant inlet header pipe, and the first refrigerant water outlet is connected with a refrigerant circulating pump through a pipeline. The refrigerant circulating pump is connected to the first inlet end of the cold using device through a pipeline, the refrigerant inlet header pipe is connected to the second inlet end of the cold using device through a pipeline, the outlet end of the cold using device is communicated with the second refrigerant water inlet, and the second refrigerant water outlet is connected with a refrigerant water return pipe. Large temperature difference can be eliminated, seasonal-difference-free stable high-yield acid production is realized, the fixed flow of a refrigerant is kept, the temperature difference between the refrigerant and a medium in the kettle is accurately controlled, and the upper temperature and the lower temperature of the medium in the kettle are basically consistent.

This invention relates to the field of feed, specifically disclosing a method for preparing selenium- and strontium-enriched amino acid chelated feed; including the preparation of selenium / strontium-enriched amino acid fermentation products, the preparation steps of which are as follows: S1: Inoculating activated microalgae and yeast strains into a culture medium supplemented with selenium and strontium compounds, the pH of the culture medium is 6.5-7.5, the culture temperature is 25-35℃, and the culture is carried out under light and aeration conditions for 5-7 days; S2: Collecting selenium / strontium-enriched algal biomass using a flocculant, and ultrasonically treating the selenium / strontium-enriched algal biomass for 10-20 minutes, then adding soybean meal and rapeseed cake to the selenium / strontium-enriched algal biomass and stirring; S3: Adding cellulase, papain, alkaline protease, and pectinase for enzymatic hydrolysis to obtain selenium / strontium-enriched amino acid fermentation products. The selenium- and strontium-enriched amino acid chelated feed and its preparation method of this invention can improve the intestinal health of animals, increase the absorption and utilization rate of nutrients, and enhance animal immunity.

The utility model discloses an amino acid fermentation tail gas treatment device, which relates to the technical field of amino acid fermentation and comprises a fermentation pipe, the fermentation pipe is fixedly connected with a pretreatment filtering tank through an inverted U-shaped exhaust pipe, and the bottom end of the pretreatment filtering tank is fixedly connected with a chemical absorption tank through an air inlet pipe. The top end of the chemical absorption tank is fixedly connected with an activated carbon treatment box through a pipeline, and the inner wall of the chemical absorption tank is fixedly connected with a first ring pipe and a second ring pipe. Through the unique sand clock type flow guide pipe and the design of the filter screens with different pore diameters in the pretreatment filter tank, dust in tail gas is efficiently filtered in a classified mode according to the aerodynamic principle, it is ensured that more than 90% of particles with the specific size larger than 10 micrometers are intercepted, and follow-up equipment pipelines are effectively prevented from being blocked and abraded. And meanwhile, the chemical absorption tank creatively adopts a mode of combining a double-layer ring pipe inclined spray head and a rotary spray head, so that the alkaline absorbent is in full contact with the tail gas.

The invention belongs to the technical field of biology, and discloses a neutral protease mutant, the amino acid sequence of which is mutated on the basis of the amino acid sequence of neutral protease as shown in SEQ ID NO: 1: (1) substitution of 50th amino acid alanine into aspartic acid, (2) substitution of 171st valine into leucine, or (3) substitution of 50th amino acid alanine into aspartic acid, or (4) substitution of 171st valine into leucine, or (5) substitution of 171st valine into leucine, or (6) substitution of 171st valine into leucine, or (7) substitution of 171st valine into leucine. And valine at the 171st site is replaced by leucine. Compared with a wild type, the neutral protease mutant disclosed by the invention has the advantages that the enzyme activity is improved, the optimum pH is lower, the stability is improved, and the neutral protease mutant is suitable for repairing amino acid wastewater with lower pH.

The application provides a fermentation tank feeding device for amino acid production and a use method, and belongs to the technical field of amino acid fermentation production equipment, and comprises a knocking mechanism, the knocking mechanism comprises a fermentation tank body, a lifting device is arranged on one side of the fermentation tank body, a hopper is arranged on the lifting device, a discharging plate is arranged at the lower part of the hopper, a lower frame is arranged outside the discharging plate, a vibrating hammer is arranged on the lower frame, a transmission assembly is arranged on one side of the vibrating hammer, and the transmission assembly is used for driving the vibrating hammer to knock off the wall-hanging material on the inner wall of the hopper; a frequency adjusting mechanism comprises an upper frame fixedly arranged on the upper part of the hopper, and a lifting mechanism is arranged at the lower part of the upper frame, and the lifting mechanism is used for changing the knocking frequency of the vibrating hammer on the hopper according to the difference between dry and wet materials. The application solves the problems of material waste caused by wall-hanging of materials and feeding caused by different wall-hanging characteristics of dry and wet materials.

This invention discloses a strain that produces high levels of L-homoserine, its construction method, and its applications, belonging to the field of amino acid fermentation. This invention provides a strain for efficient fermentation production of L-homoserine, its construction method, and its applications. This strain utilizes a cofactor homogenization scheme to introduce a heterologous NADH-dependent dehydrogenase to replace the original NADPH-dependent dehydrogenase in *E. coli*, thereby removing cofactor limitations, knocking out the main byproduct pathway, enhancing precursor supply, and strengthening efflux protein expression. Fed-batch fermentation in a fermenter achieves a yield as high as 155 g / L.

The application provides a kind of MurJ protein mutant and its application.The mutant includes at least one of the following ①-③ mutations: ① the mutation of the 373th amino acid of MurJ protein from P to S;② the mutation of the 422th amino acid of MurJ protein from A to T;③ the mutation of the 882th amino acid of MurJ protein from T to I.The MurJ protein variant provided by the application containing P373S, A422T, T882I mutation site can improve the yield and conversion rate of amino acid, and also can ensure the growth performance of strain.Using the recombinant microorganism expressing the above-mentioned MurJ protein variant for amino acid fermentation production, the amino acid fermentation production performance is significantly improved, especially glutamic acid, lysine, proline, with high yield and sugar acid conversion rate, providing a powerful tool for large-scale industrial production of amino acid.

The present application relates to the technical field of genetic engineering, in particular to transcriptional regulator PcaR, mutant thereof and engineering bacteria thereof. The present application finds that the transcriptional regulator PcaR has the function of regulating the production of amino acids such as threonine, and can be used as a metabolic modification target point of amino acid production strains. The present application provides a mutant of the transcriptional regulator PcaR, which can significantly improve the production capacity of bacteria for amino acids such as threonine. The threonine yield and conversion rate of the engineering bacteria constructed based on the mutant are significantly improved, and the engineering bacteria have good application prospects in the field of amino acid fermentation.

The present application relates to the technical field of wheat bio-organic foliar fertilizer, and discloses a wheat zinc-selenium bio-organic foliar fertilizer and a preparation method thereof, which comprises: 300-400 parts of fermentation substrate, 40-60 parts of zinc sulfate, 2-5 parts of sodium selenite, 15-25 parts of alginic acid extract, 80-120 parts of compound amino acid, 1-2 parts of potassium sorbate and 435-554 parts of water. The present application combines zinc-selenium nutrient fortification with the biological stimulation of alginic acid extract, compound amino acid and fermentation substrate. The alginic acid extract can promote the growth of wheat root system, improve the photosynthetic efficiency of leaves and enhance the drought resistance and disease resistance of wheat. The compound amino acid can be directly absorbed and utilized by wheat, supplement the raw materials for protein synthesis and improve the quality of grains. The organic matter and beneficial bacteria metabolites in the fermentation substrate can regulate the growth rhythm of wheat, promote nutrient transport, and achieve the effect of trinity of wheat nutrient fortification, stress resistance enhancement and yield increase.

This paper discloses the use of modified Escherichia coli and its L-amino acid fermentation production. Specifically, it discloses the use of pyruvate formate lyase, alcohol dehydrogenase, branched-chain amino acid transaminase derived from Escherichia coli, branched-chain amino acid transaminase derived from Bacillus subtilis, thiamine phosphate synthase, and maleate dehydrogenase in the construction of genetically modified bacteria that produce L-amino acids. Experiments have shown that by knocking out the genes encoding pyruvate formate lyase, alcohol dehydrogenase, branched-chain amino acid transaminase derived from Escherichia coli, thiamine phosphate synthase, and maleate dehydrogenase in E. coli capable of producing L-amino acids, and introducing the gene encoding branched-chain amino acid transaminase derived from Bacillus subtilis, recombinant bacteria favorable for L-amino acid accumulation can be obtained. These recombinant bacteria can significantly increase L-amino acid production and reduce costs, which is of great significance in accelerating the industrialization process of L-amino acids.

A CFD-based optimization method for a fermenter for amino acid fermentation according to an embodiment of the present invention includes: a first step of setting an objective variable, a constraint variable, and a design variable with respect to an optimization objective of at least one impeller fermenter; a second step of generating a three-dimensional shape for a flow region of the impeller fermenter for each condition of the constraint variable and the design variable according to the objective variable; a third step of calculating a primary value, which is at least one of oxygen diffusion coefficient, liquid density, liquid viscosity, gas hold-up, bubble diameter, energy dissipation rate, and torque, by computational fluid dynamics analysis for the flow region based on information about the three-dimensional shape; a fourth step of calculating secondary values, which are an oxygen transfer coefficient and a power consumption ratio (power ratio), using the primary value; and a fifth step of repeating the second, third, and fourth steps while varying the design variable of the first step in various ways, and finding an optimal point based on a comparison value obtained by comparing the secondary values before and after the variation.

The utility model relates to the technical field of amino acid production, in particular to a sealed centrifugal machine for amino acid production, which comprises a flat plate base, a centrifugal machine box is fixedly connected to the middle of the top of the flat plate base, a sealed upper cover is clamped at the top of the centrifugal machine box, and a pretreatment sleeve is arranged at the right position of the top of the sealed upper cover. A ceramic conical disc is arranged in the middle of the interior of the pretreatment sleeve, and the inner clamping sleeve, the upper conical ring, the ceramic conical disc, the annular temperature controller, the electrifying terminal, the electric heating needle head and the supporting block are arranged; according to the sealed centrifugal machine facilitating amino acid production, before amino acid fermentation liquor is fed into the separation drum body through the feeding pipe to be treated, the amino acid fermentation liquor with the high viscosity is preheated through the preheating structure, the viscosity of the amino acid fermentation liquor body is reduced, and the situation that due to the high viscosity of the amino acid fermentation liquor, the viscosity of the amino acid fermentation liquor is reduced is avoided. The separation settling speed is slowed down and the separation efficiency is reduced during the operation of the equipment.

The present application relates to a kind of biogas slurry-based fermentation broth production device and production method, solve the existing biogas slurry component complex, it is difficult to use, smell is unpleasant, acceptance is not high, and the problem of high cost of biogas slurry processing.This device greenhouse is set up above fermentation tank, and the middle part of fermentation tank is provided with shunt wall along the length direction, and the both ends of shunt wall are left empty between the end wall of fermentation tank, and one end of fermentation tank is provided with recessed secondary pool, and the both sides and both ends of fermentation tank form the circulation channel of biogas slurry on the both sides of shunt wall, and water removal car is arranged on the circulation channel, and the liquid inlet pipe is arranged on the secondary pool of fermentation tank, and the liquid outlet pipe is arranged in secondary pool, and the dosing pipe of adding composite microbial inoculant is further arranged above secondary pool.The present application forms efficient composite microorganism-amino acid fermentation broth product after fermentation treatment to biogas slurry, which can partially replace chemical fertilizer, greatly reduces the processing cost of biogas slurry, reduces the economic burden of breeder, and reduces the problem of biogas slurry environmental pollution.

The utility model relates to amino acid fermentation liquor processing technical field discloses a disc type separator, including separator main part, the upper end of separator main part is fixedly connected with feed pipe and discharge pipe respectively, pretreatment subassembly, pretreatment subassembly sets up between separator main part and feed pipe, pretreatment subassembly is used for the pretreatment of amino acid fermentation liquor, and pretreatment subassembly includes installation shell, mixing pipe and driving motor, and through the setting of separator main part and pretreatment subassembly realizes the coherent realization of the pretreatment before amino acid fermentation liquor processing and the separation step of amino acid fermentation liquor, reduces processing step, improves processing efficiency, the heating wire in installation shell is electrified heating after the device works, and the enzyme in amino acid fermentation liquor is inactivated by heating the amino acid fermentation liquor that flows through the installation shell, prevents the target object inactivation, and the quantitative amino acid fermentation liquor and treating agent are sent into the mixing pipe through the conveying pipe.

The application belongs to the field of separation and purification of organic compounds, and discloses a method for extracting amino acids from an amino acid fermentation liquor by using an ion rectification system. By accurately controlling the pH value of the amino acid fermentation liquor, the charged states of amino acid ions and inorganic ions are made different, and by using a special membrane arrangement mode of the ion rectification technology, efficient separation of multiple components in the amino acid fermentation liquor is realized. Meanwhile, the ability of the bipolar membrane to dissociate water molecules is combined to recover the corresponding acid and base. The application provides an innovative method for extracting amino acids from an amino acid fermentation liquor, realizes accurate separation of amino acids in the fermentation liquor, simultaneously improves the recovery efficiency and product purity, reduces waste discharge, and greatly reduces the production cost of amino acids.

This invention belongs to the field of bioengineering technology, specifically relating to a nutritional composition, its preparation method, and its application. By weight, the nutritional composition comprises 80-98 parts yeast extract and 1-15 parts free methionine. It also includes 0.1-6 parts choline chloride, 0.1-2 parts niacin, 0.01-0.15 parts vitamin B2, 0.01-0.2 parts vitamin B6, 0.01-0.25 parts vitamin B5, 0.001-0.05 parts vitamin B1, 0.001-0.06 parts vitamin C, and 0.0001-0.0008 parts biotin. The peptides with a molecular weight of 1000 Da or less account for at least 70% of the total peptides in the nutritional composition. The nutritional composition provided by this invention can be efficiently utilized by the producing strains during the production of amino acid fermentation broth, resulting in fewer impurities in the fermentation broth and facilitating the extraction of fermentation products. Simultaneously, it shortens the fermentation cycle of the amino acid fermentation broth, increases the amino acid concentration, and improves the sugar-acid conversion rate.