Preparation method of selenium-rich strontium amino acid chelate feed

Abstract

This invention relates to the field of feed, specifically disclosing a method for preparing selenium- and strontium-enriched amino acid chelated feed; including the preparation of selenium / strontium-enriched amino acid fermentation products, the preparation steps of which are as follows: S1: Inoculating activated microalgae and yeast strains into a culture medium supplemented with selenium and strontium compounds, the pH of the culture medium is 6.5-7.5, the culture temperature is 25-35℃, and the culture is carried out under light and aeration conditions for 5-7 days; S2: Collecting selenium / strontium-enriched algal biomass using a flocculant, and ultrasonically treating the selenium / strontium-enriched algal biomass for 10-20 minutes, then adding soybean meal and rapeseed cake to the selenium / strontium-enriched algal biomass and stirring; S3: Adding cellulase, papain, alkaline protease, and pectinase for enzymatic hydrolysis to obtain selenium / strontium-enriched amino acid fermentation products. The selenium- and strontium-enriched amino acid chelated feed and its preparation method of this invention can improve the intestinal health of animals, increase the absorption and utilization rate of nutrients, and enhance animal immunity.

Description

Technical Field

[0001] This invention relates to the field of feed, specifically to a method for preparing selenium-enriched strontium amino acid chelated feed. Background Technology

[0002] In modern animal husbandry, feed quality directly impacts animal growth performance, health status, and the yield and quality of final products. With increasing public concern for food safety and nutritional health, developing feeds with specific nutritional functions or health-promoting effects has become a significant industry trend. Selenium and strontium are essential trace elements for animal life, playing crucial roles in maintaining normal physiological functions, enhancing immunity, and promoting bone development. However, in traditional feed formulations, the amount of these trace elements added is often difficult to control precisely and is easily affected by fluctuations in the trace element content of raw materials, thus impacting the overall nutritional value of the feed.

[0003] Furthermore, gut health is crucial for an animal's growth and overall health. The gut is not only the primary site of nutrient absorption but also an important component of the body's immune system. Therefore, there is a need for feed that can improve an animal's gut health and enhance its absorption and utilization of nutrients. Summary of the Invention

[0004] The present invention aims to provide a selenium- and strontium-rich chelated feed and its preparation method to improve the intestinal health of animals, enhance the absorption and utilization rate of nutrients, and improve animal immunity.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a method for preparing selenium-enriched strontium amino acid chelated feed, comprising the preparation of selenium / strontium amino acid fermentation products, wherein the preparation steps are as follows:

[0006] S1: Cultivation of Yeast in Selenium / Strontium Algae Solution

[0007] The activated microalgae and yeast strains were inoculated into a culture medium supplemented with selenium and strontium compounds. The pH of the culture medium was 6.5–7.5, the culture temperature was 25–35℃, and the culture was carried out for 5–7 days under light and ventilation conditions.

[0008] S2: Preparation of fermentation feedstock by enzymatic hydrolysis

[0009] Selenium-enriched / strontium-enriched algae biomass was obtained by harvesting with flocculants, and the selenium-enriched / strontium-enriched algae biomass was ultrasonically treated for 10-20 minutes. Then, crushed soybean meal and rapeseed cake were added to the selenium-enriched / strontium-enriched algae biomass and stirred for 5-15 minutes.

[0010] S3: Preparation of selenium-enriched / strontium amino acid fermentation products

[0011] Cellulase, papain, alkaline protease, and pectinase were added for enzymatic hydrolysis to obtain a selenium-enriched / strontium amino acid ferment.

[0012] Furthermore, in step S1, the concentration of the selenium compound is 1–2 mg / L and the concentration of the strontium compound is 1–3 mg / L.

[0013] Furthermore, the amount of microalgae added in step S1 is 20wt% to 80wt% of the yeast weight.

[0014] Furthermore, in step S2, the weight ratio of selenium-enriched / strontium-rich algae biomass, soybean meal, and rapeseed cake is 25–40:40–60:20–30.

[0015] Further, during enzymatic hydrolysis in step S3, 0.05%–0.1% cellulase, 0.1%–0.15% papain, 0.1%–0.2% alkaline protease, and 0.1%–0.15% pectinase are added, and enzymatic hydrolysis is carried out for 1–5 hours at a temperature of 48–60°C and a pH of 6.0–6.4.

[0016] Furthermore, it includes a feed preparation step, which is shown below:

[0017] S4: Mix the selenium-enriched / strontium-enriched amino acid fermentation product and element additives, then add an initiator, and catalyze the reaction with microwave for 30-120 minutes. After washing and drying, the selenium-enriched / strontium-enriched amino acid trace element chelate is obtained.

[0018] S5: Selenium-enriched / strontium amino acid trace element chelate, compound flour, compound biological agent and compound vitamin are mixed to obtain selenium-enriched / strontium amino acid chelate feed.

[0019] Furthermore, the above-mentioned selenium-enriched strontium amino acid chelated feed includes the following raw materials in parts by weight: 40-55 parts of selenium-enriched / strontium amino acid fermentation product, 35-45 parts of compound flour, 5-10 parts of elemental additives, 0.5-2 parts of compound biological agent, and 0.1-1 parts of compound vitamins.

[0020] Furthermore, the elemental additives include the following raw materials in parts by weight: 30-55 parts calcium chloride, 25-42 parts magnesium chloride, 5-10 parts zinc acetate, and 2-5 parts ferric chloride.

[0021] Furthermore, compound flour includes wheat flour, corn flour, and bone meal.

[0022] Furthermore, the compound biological agent includes Clostridium butyricum and lactic acid bacteria.

[0023] This invention also discloses

[0024] The beneficial effects of this plan are:

[0025] (1) The feed prepared by this technical solution can effectively improve the growth rate and survival rate of farmed animals, thereby increasing the economic benefits of aquaculture enterprises. Selenium and strontium are essential trace elements for animal growth, and the amino acid chelate form can improve the bioavailability of these elements. Selenium has antioxidant effects, which can protect cells from oxidative damage and promote metabolism; strontium helps bone development and muscle function. Thus, the overall growth rate of farmed animals is improved. Secondly, Clostridium butyricum and lactic acid bacteria can regulate the balance of intestinal flora, inhibit the growth of harmful bacteria, and enhance the immune function of animals. Clostridium butyricum can also produce butyric acid, which promotes the repair and regeneration of intestinal cells. The selenium-enriched / strontium amino acid chelate provides essential trace elements and antioxidant protection, while the compound biological agent enhances the absorption and utilization efficiency of these elements by improving intestinal health. The combination of the two not only improves the growth rate and survival rate, but also enhances the overall health of fish.

[0026] (2) The preparation method of this technical solution is simple, the raw materials are widely available and relatively inexpensive, and the production process is simple and low-cost. This feed formula is suitable for a variety of aquatic animals, including fish, shrimp and shellfish, and has a wide range of applications. Solid-phase microwave technology is suitable for large-scale industrial production, with relatively low equipment investment and simple operation, making it suitable for aquaculture enterprises of all sizes.

[0027] (3) The microalgae strain can be Chlorella. Compared with existing preparation methods, this invention achieves the enrichment of selenium / strontium by co-culturing microalgae and yeast in a selenium / strontium-containing culture medium. Selenium / strontium-enriched amino acid trace element chelates were successfully prepared using solid-phase microwave technology. The preparation method of this invention has significant advantages in improving the bioavailability of trace elements, promoting feed digestibility and absorption, and enhancing animal immunity. These advantages not only improve the nutritional value of feed but also bring greater economic benefits to enterprises. Attached Figure Description

[0028] Figure 1 This is a flowchart illustrating the preparation method of selenium-enriched / strontium amino acid chelated feed in this invention embodiment;

[0029] Figure 2 The growth of microalgae-yeast in different selenium-containing culture media;

[0030] Figure 3 This shows the amount of selenium absorbed by microalgae and yeast.

[0031] Figure 4 The growth of microalgae-yeast in different strontium-containing culture media;

[0032] Figure 5 This shows the amount of strontium absorbed by microalgae-yeast. Detailed Implementation

[0033] The following detailed description illustrates the specific implementation method:

[0034] Example

[0035] This invention discloses a method for preparing selenium-enriched strontium amino acid chelated feed, which uses the following raw materials in parts by weight: 40-55 parts of selenium-enriched / strontium amino acid fermentation product, 35-45 parts of compound flour, 5-10 parts of elemental additives, 0.5-2 parts of compound biological agent, and 0.1-1 parts of compound vitamins.

[0036] The elemental additives include the following raw materials in parts by weight: 30-55 parts calcium chloride, 25-42 parts magnesium chloride, 5-10 parts zinc acetate, and 2-5 parts ferric chloride.

[0037] Compound flour includes wheat flour, corn flour, and bone meal; compound biological agents include Clostridium butyricum and lactic acid bacteria, and the colony-forming units of Clostridium butyricum are ≥60×10⁻⁶. 8 CFU / g, Lactic acid bacteria colony forming units ≥100×10⁻⁶ 8 cfu / g.

[0038] This invention discloses a method for preparing selenium-enriched strontium amino acid chelated feed. First, a selenium-enriched / strontium amino acid ferment is prepared. The preparation steps for the selenium-enriched / strontium amino acid ferment are as follows:

[0039] S1: Cultivation of Yeast in Selenium / Strontium Algae Solution

[0040] The activated microalgae and yeast strains were inoculated into a culture medium containing 1–2 mg / L selenium compounds and 1–3 mg / L strontium compounds. The pH of the culture medium was 6.5–7.5, the inoculation amount was 5–10%, and the culture temperature was 25–35℃. The culture was carried out for 5–7 days under light and aeration conditions. The amount of microalgae added was 20 wt%–80 wt% of the weight of the yeast.

[0041] S2: Preparation of fermentation feedstock by enzymatic hydrolysis

[0042] Using existing technology, selenium-enriched / strontium-enriched algae biomass is obtained by harvesting with flocculants; the selenium-enriched / strontium-enriched algae biomass is ultrasonically treated for 10-20 minutes, and then crushed soybean meal and rapeseed cake are added to the selenium-enriched / strontium-enriched algae biomass and stirred for 5-15 minutes;

[0043] The flocculant is one or both of chitosan and magnesium chloride;

[0044] The weight ratio between selenium-enriched / strontium-enriched algae biomass and the added soybean meal and rapeseed cake is 25-40:40-60:20-30;

[0045] S3: Preparation of selenium-enriched / strontium amino acid fermentation products

[0046] Add 0.05%–0.1% cellulase, 0.1%–0.15% papain, 0.1%–0.2% alkaline protease, and 0.1%–0.15% pectinase, and enzymatically hydrolyze for 1–5 hours at a temperature of 48–60℃ and a pH of 6.0–6.4 to obtain a selenium / strontium amino acid ferment with a crude protein content ≥45%, a selenium concentration of 15–30 μg / g, a strontium concentration of 25–35 μg / g, and a chlorophyll content ≥2.5 mg / g.

[0047] Feed preparation includes the following steps:

[0048] S4: Mix the selenium-enriched / strontium-enriched amino acid fermentation product and element additives, then add an initiator, and catalyze the reaction with microwave for 30-120 minutes. After washing and drying, the selenium-enriched / strontium-enriched amino acid trace element chelate is obtained.

[0049] S5: Selenium-enriched / strontium amino acid trace element chelate, compound flour, compound biological agent and compound vitamins are mixed to obtain selenium-enriched / strontium amino acid chelate feed; compound vitamins include vitamin C and B vitamins.

[0050] This invention discloses Examples 1 to 3, and the weight parts of each raw material in Examples 1 to 3 are shown in the table below:

[0051]

[0052] The preparation method of Example 1 is as follows:

[0053] Preparation of selenium / strontium amino acid fermentation products

[0054] S1: Cultivation of Yeast in Selenium / Strontium Algae Solution

[0055] The activated microalgae and yeast strains were inoculated into a culture medium with a concentration of 1-2 mg / L of selenium compounds and 1-3 mg / L of strontium compounds. The pH of the culture medium was 6.5-7.5, the inoculation amount was 10%, the culture temperature was 25℃, and the culture was carried out for 5 days under light and aeration conditions. The amount of microalgae added was 20wt%-80wt% of the weight of yeast.

[0056] S2: Preparation of fermentation feedstock by enzymatic hydrolysis

[0057] Selenium-enriched / strontium-enriched algae biomass was obtained by harvesting with flocculants. The selenium-enriched / strontium-enriched algae biomass was ultrasonically treated for 20 minutes. Then, crushed soybean meal and rapeseed cake were added to the selenium-enriched / strontium-enriched algae biomass and stirred for 15 minutes.

[0058] The flocculant is one or both of chitosan and magnesium chloride;

[0059] The weight ratio between selenium-enriched / strontium-enriched algae biomass and the added soybean meal and rapeseed cake is 25-40:40-60:20-30;

[0060] S3: Preparation of selenium-enriched / strontium amino acid fermentation products

[0061] Add 0.05% cellulase, 0.1% papain, 0.1% alkaline protease and 0.1% pectinase, and enzymatically hydrolyze for 1 hour at 55℃ and pH 6.0 to obtain a selenium / strontium amino acid ferment with a crude protein content ≥45%, a selenium concentration of 15-30 μg / g, a strontium concentration of 25-35 μg / g, and a chlorophyll content ≥2.5 mg / g.

[0062] Feed preparation

[0063] S4: Mix the selenium-enriched / strontium-enriched amino acid fermentation product and element additives, then add an initiator, and catalyze the reaction with microwave for 30-120 minutes. After washing and drying, the selenium-enriched / strontium-enriched amino acid trace element chelate is obtained.

[0064] S5: Selenium-enriched / strontium amino acid trace element chelate, compound flour, compound biological agent and compound vitamins are mixed to obtain selenium-enriched / strontium amino acid chelate feed; compound vitamins include vitamin C and B vitamins.

[0065] The preparation method of Example 2 is as follows:

[0066] Preparation of selenium / strontium amino acid fermentation products

[0067] S1: Cultivation of Yeast in Selenium / Strontium Algae Solution

[0068] The activated microalgae and yeast strains were inoculated into a culture medium with a concentration of 1-2 mg / L of selenium compound and 1-3 mg / L of strontium compound. The pH of the culture medium was 6.5-7.5, the inoculation amount was 10%, the culture temperature was 30℃, and the culture was carried out for 5 days under light and aeration conditions. The amount of microalgae added was 20 wt% of the weight of yeast.

[0069] S2: Preparation of fermentation feedstock by enzymatic hydrolysis

[0070] Selenium-enriched / strontium-enriched algae biomass was obtained by harvesting with flocculants; the selenium-enriched / strontium-enriched algae biomass was ultrasonically treated for 10 minutes, and then crushed soybean meal and rapeseed cake were added to the selenium-enriched / strontium-enriched algae biomass and stirred for 15 minutes.

[0071] The flocculant is one or both of chitosan and magnesium chloride;

[0072] The weight ratio between selenium-enriched / strontium-enriched algae biomass and the added soybean meal and rapeseed cake is 25-40:40-60:20-30;

[0073] S3: Preparation of selenium-enriched / strontium amino acid fermentation products

[0074] Adding 0.1% of cellulase, 0.1% of papain, 0.1% of alkaline protease, and 0.1% of pectinase to the fermentation feedstock containing cellulase, papain, alkaline protease, and pectinase, and enzymatically hydrolyzing for 5 hours at 60℃ and pH 6.4, yields a selenium / strontium amino acid ferment with a crude protein content ≥45%, a selenium concentration of 15–30 μg / g, a strontium concentration of 25–35 μg / g, and a chlorophyll content ≥2.5 mg / g.

[0075] Feed preparation

[0076] S4: Mix the selenium-enriched / strontium-enriched amino acid fermentation product and element additives, then add an initiator, and catalyze the reaction with microwave for 30-120 minutes. After washing and drying, the selenium-enriched / strontium-enriched amino acid trace element chelate is obtained.

[0077] S5: Selenium-enriched / strontium amino acid trace element chelate, compound flour, compound biological agent and compound vitamins are mixed to obtain selenium-enriched / strontium amino acid chelate feed; compound vitamins include vitamin C and B vitamins.

[0078] The preparation method of Example 3 is as follows:

[0079] Preparation of selenium / strontium amino acid fermentation products

[0080] S1: Cultivation of Yeast in Selenium / Strontium Algae Solution

[0081] The activated microalgae and yeast strains were inoculated into a culture medium with a selenium concentration of 1-2 mg / L and a strontium concentration of 1-3 mg / L. The pH of the culture medium was 6.5-7.5, the inoculation amount was 10%, the culture temperature was 35℃, and the culture was carried out for 7 days under light and aeration conditions. The amount of microalgae added was 20wt%-80wt% of the weight of yeast.

[0082] S2: Preparation of fermentation feedstock by enzymatic hydrolysis

[0083] Selenium-enriched / strontium-enriched algae biomass was obtained by harvesting with flocculants; the selenium-enriched / strontium-enriched algae biomass was ultrasonically treated for 10 minutes, and then crushed soybean meal and rapeseed cake were added to the selenium-enriched / strontium-enriched algae biomass and stirred for 15 minutes.

[0084] The flocculant is one or both of chitosan and magnesium chloride;

[0085] The weight ratio between selenium-enriched / strontium-enriched algae biomass and the added soybean meal and rapeseed cake is 25-40:40-60:20-30;

[0086] S3: Preparation of selenium-enriched / strontium amino acid fermentation products

[0087] Adding 0.1% cellulase, 0.15% papain, 0.2% alkaline protease, and 0.15% pectinase to the fermentation feedstock containing cellulase, papain, alkaline protease, and pectinase, and enzymatically hydrolyzing for 5 hours at 60℃ and pH 6.4, yields a selenium / strontium amino acid ferment with a crude protein content ≥45%, a selenium concentration of 15–30 μg / g, a strontium concentration of 25–35 μg / g, and a chlorophyll content ≥2.5 mg / g.

[0088] Feed preparation

[0089] S4: Mix the selenium-enriched / strontium-enriched amino acid fermentation product and element additives, then add an initiator, and catalyze the reaction with microwave for 30-120 minutes. After washing and drying, the selenium-enriched / strontium-enriched amino acid trace element chelate is obtained.

[0090] S5: Selenium-enriched / strontium-enriched amino acid trace element chelate, compound flour, compound biological agent, and compound vitamins are mixed to obtain selenium-enriched / strontium-enriched amino acid chelate feed; the compound vitamins include vitamin C and B vitamins.

[0091] Comparative Examples 1 to 5 are also disclosed in this embodiment:

[0092] Comparative Example 1

[0093] Based on Example 2, except for the amount of microalgae added, all other components and preparation methods are the same as in Example 2. The amount of microalgae added is 25 wt% of the yeast.

[0094] Comparative Example 2

[0095] Based on Example 2, except for the amount of microalgae added, all other components and preparation methods are the same as in Example 2. The amount of microalgae added is 50 wt% of the yeast weight.

[0096] Comparative Example 3

[0097] Based on Example 2, except for the amount of microalgae added, all other components and preparation methods are the same as in Example 2. The amount of microalgae added is 75 wt% of the yeast.

[0098] Comparative Example 4

[0099] Based on Example 2, except for the amount of selenium compound added, the other components and preparation methods are the same as in Example 2.

[0100] Comparative Example 5

[0101] Based on Example 2, except for the amount of strontium compound added, the other components and preparation methods are the same as in Example 2.

[0102] The selenium-enriched / strontium amino acid chelated feeds prepared using Examples 2 and Comparative Examples 1-3 were fed to fish of the same species and age under the same environmental and water quality conditions. The effects of the feeds on fish growth were tested, and the results are shown in the table below:

[0103]

[0104] The first group above was fed pure fish meal. The feed of the present invention significantly improved the survival rate and weight of the fish. When the amount of microalgae added was 75 wt% of the weight of yeast, the survival rate and weight of the fish were significantly improved.

[0105] Simultaneously, the growth of microalgae-yeast in different selenium-containing and strontium-containing media was detected. Comparative examples 4-5 show the results. Figures 2-5 The growth and uptake of microalgae-yeast were optimal when the selenium and strontium concentrations were 10 mg / L and 20 mg / L, respectively.

[0106] The above descriptions are merely embodiments of the present invention, and common knowledge such as specific technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solutions of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A method for preparing a selenium-enriched strontium amino acid chelated feed, characterized in that: The preparation of selenium-enriched / strontium amino acid ferments includes the following steps: S1: Cultivation of Yeast in Selenium / Strontium Algae Solution The activated microalgae and yeast strains were inoculated into a culture medium supplemented with selenium and strontium compounds. The pH of the culture medium was 6.5–7.5, the culture temperature was 25–35℃, and the culture was carried out for 5–7 days under light and ventilation conditions. S2: Preparation of fermentation feedstock by enzymatic hydrolysis Selenium-enriched / strontium-enriched algae biomass was obtained by harvesting with flocculants, and the selenium-enriched / strontium-enriched algae biomass was ultrasonically treated for 10-20 minutes. Then, crushed soybean meal and rapeseed cake were added to the selenium-enriched / strontium-enriched algae biomass and stirred for 5-15 minutes. S3: Preparation of selenium-enriched / strontium amino acid fermentation products Cellulase, papain, alkaline protease, and pectinase were added for enzymatic hydrolysis to obtain a selenium-enriched / strontium amino acid ferment.

2. The method for preparing a selenium-enriched strontium amino acid chelated feed according to claim 1, characterized in that: In step S1, the concentration of selenium compound is 1-2 mg / L and the concentration of strontium compound is 1-3 mg / L.

3. The method for preparing a selenium-enriched strontium amino acid chelated feed according to claim 1, characterized in that: The amount of microalgae added in step S1 is 20wt% to 80wt% of the yeast weight.

4. The method for preparing a selenium-enriched strontium amino acid chelated feed according to claim 1, characterized in that: In step S2, the weight ratio of selenium-enriched / strontium-enriched algae biomass, soybean meal, and rapeseed cake is 25–40:40–60:20–30.

5. The method for preparing a selenium-enriched strontium amino acid chelated feed according to claim 1, characterized in that: In step S3, 0.05%–0.1% cellulase, 0.1%–0.15% papain, 0.1%–0.2% alkaline protease, and 0.1%–0.15% pectinase are added for enzymatic hydrolysis. The hydrolysis is carried out at a temperature of 48–60℃ and a pH of 6.0–6.4 for 1–5 hours.

6. The method for preparing a selenium-enriched strontium amino acid chelated feed according to claim 1, characterized in that: The feed preparation steps are as follows: S4: Mix the selenium-enriched / strontium-enriched amino acid fermentation product and element additives, then add an initiator, and catalyze the reaction with microwave for 30-120 minutes. After washing and drying, the selenium-enriched / strontium-enriched amino acid trace element chelate is obtained. S5: Selenium-enriched / strontium amino acid trace element chelate, compound flour, compound biological agent and compound vitamin are mixed to obtain selenium-enriched / strontium amino acid chelate feed.

7. The method for preparing a selenium-enriched strontium amino acid chelated feed according to claim 1, characterized in that: The ingredients include the following parts by weight: 40-55 parts of selenium-enriched / strontium amino acid fermentation product, 35-45 parts of compound flour, 5-10 parts of element additives, 0.5-2 parts of compound biological agent, and 0.1-1 parts of compound vitamins.

8. The method for preparing a selenium-enriched strontium amino acid chelated feed according to claim 1, characterized in that: The elemental additives include the following raw materials in parts by weight: 30-55 parts calcium chloride, 25-42 parts magnesium chloride, 5-10 parts zinc acetate, and 2-5 parts ferric chloride.

9. A selenium- and strontium-rich amino acid chelated feed according to claim 1, characterized in that: Compound flour includes wheat flour, corn flour, and bone meal.

10. A selenium- and strontium-rich amino acid chelated feed according to claim 1, characterized in that: The compound biological agent includes Clostridium butyricum and lactic acid bacteria.

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