Method for preparing gamma-polyglutamic acid through soild fermentation by utilizing edible fungi residues

Abstract

The invention discloses a method for preparing gamma-polyglutamic acid through solid fermentation by utilizing edible fungi residues. The method comprises the following steps: (1) preparation of solid fungicide: collecting thallus in gamma- polyglutamic acid fermentation liquor to be mixed with a carrier, and drying to obtain the solid fungicide; (2) solid fermentation: inoculating the solid fungicide obtained from the step (1) into a fermentation medium to be mixed uniformly, and fermenting to prepare the gamma-polyglutamic acid, wherein the fermentation medium consists of a solid matrix and an exogenous additive, and the solid matrix is a mixture of the edible fungi residues and aginomoto. Compared with the prior art in which gamma-polyglutamic acid is produced through solid fermentation, the method disclosed by the invention is used for efficiently producing gamma-polyglutamic acid, the production cost is greatly reduced through the utilization of agricultural waste residues, meanwhile, a new way is found for the disposal of the residues, and the environment pollution problem caused by the residues is avoided. Therefore, the method is a high-efficient, low-cost, environment-friendly biological reaction process and has good industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a method for high-yielding γ-polyglutamic acid by utilizing edible fungus residues for solid fermentation. Background technique [0002] γ-polyglutamic acid (γ-poly-glutamic acid, referred to as γ-PGA) is a polyamino acid in which glutamic acid monomer is condensed with γ-carboxyl group and α-amino group (the structure is as follows). The molecular weight distribution is between 100KDa and 10000KDa. [0003] [0004] The molecular chain of γ-PGA has a large number of free side chain carboxyl groups with high activity, which can form hydrogen bonds within or between molecules, and has excellent biodegradability, film-forming, fibrogenicity, plasticity, With many unique physicochemical and biological properties such as adhesion and moisture retention, γ-PGA and its derivatives have very broad application prospects in today's environmental protection a...

AI Technical Summary

Problems solved by technology

[0005] At present, extensive research has been carried out on the fermentation production process of γ-PGA at home and abroad, mainly based on microbial liquid submerged fermentation production, but there are still many deficiencies and problems that are difficult to solve: (1). , the viscosity of the fermentation liquid increases, which limits the supply of oxygen and the transfer of quality; (2), a large amount of foam is produced in the fermentation process, which increases the difficulty of control and the probability of bacterial infection; (3), the cost of raw materials is expensive, which greatly increases the The production cost of γ-PGA; (4), the serious consumption of water resources, especially in the north where water is scarce, makes the large-scale production of γ-PGA a serious obstacle

The above studies have proved that the solid fermentation production of γ-PGA is feasible, but there are problems such as long production cycle, low production efficiency, and high cost of raw materials; therefore, a low-cost, high-efficiency solid fermentation method for producing γ-PGA Research and development are urgently needed, which will also bring a new development prospect and application platform to the fermentation industry of γ-PGA!

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