Method for preparing glutamic acid fermentation organic nitrogen additive from glutamic acid fermentation waste thalli

A technology for glutamic acid and organic nitrogen, which is applied in the field of preparing glutamic acid fermentation organic nitrogen additives, can solve problems such as environmental pollution and low economic value, and achieve the effects of solving environmental pollution problems, good income and high value.

Inactive Publication Date: 2011-02-23
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the direct discharge of organic waste water produced in the production process of monosodium glutamate will cause pollution to the environment or the direct use of its economic value is low, and to provide a method for preparing glutamic acid by using glutamic acid fermentation waste cells as raw materials. Method for fermenting organic nitrogen supplements

Method used

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  • Method for preparing glutamic acid fermentation organic nitrogen additive from glutamic acid fermentation waste thalli
  • Method for preparing glutamic acid fermentation organic nitrogen additive from glutamic acid fermentation waste thalli
  • Method for preparing glutamic acid fermentation organic nitrogen additive from glutamic acid fermentation waste thalli

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1 enzymatic hydrolysis method

[0017] (1) The effect of compound enzyme ratio on the hydrolysis of glutamic acid fermentation waste cells

[0018] Corynebacterium glutamicum was formulated into 20g / 100mL bacterial liquid, pretreated by high-pressure homogenization, homogenization conditions: homogenization 6 times under 70MPa. Add different proportions of protease and dextranase, the amount of enzyme added is 1%, the temperature is 50°C, and the recovery rate of bacterial protein is measured after hydrolysis for 10 hours. The results are shown in Table 1. It can be seen from Table 1 that when the compound ratio of protease and dextranase is 3:1, the protein recovery rate of glutamic acid fermentation waste cells is higher, and the recovery rate of acid protease and dextranase compound cell protein is the highest. : 64.27%.

[0019] Table 1 Recovery rate (%) of glutamic acid fermentation waste thalline protein under different addition ratios of compound enz...

Embodiment 2

[0026] Embodiment 2: acid hydrolysis method

[0027] (1) Effect of pH on the recovery rate of bacterial protein

[0028] Corynebacterium glutamicum was formulated into a 5 g / 100 mL bacterial solution, adjusted to pH 0.5, 1.5, 2.5, and 3.5 with 6 mol / L HCl, and hydrolyzed at 115 ° C for 20 hours. The results are shown in Table 3. It can be seen from Table 3 that pH has a great influence on acid hydrolysis. When the pH is 0.5, the protein recovery rate is nearly 60% higher than that of glutamic acid fermentation waste cells when the pH is 3.5.

[0029] Table 3 Recovery rate (%) of glutamic acid fermentation waste thalline protein under different pH

[0030]

[0031] (2) Effect of cell concentration on acid hydrolysis

[0032] Make 50ml of Corynebacterium glutamicum into 5g / 100mL, 10g / 100mL, 15g / 100mL, 20g / 100mL bacterial solution respectively, put it into a 250ml Erlenmeyer flask, add 6mol / L HCl to adjust the pH to 0.5, and hydrolyze at 115°C for 18 hours , the thalline pr...

Embodiment 3

[0035] Embodiment 3: enzyme-acid binding method

[0036] (1) After the Corynebacterium glutamicum body is washed with water, a Corynebacterium glutamicum liquid with a concentration of 20g / 100mL is prepared;

[0037] (2) Adopting a high-pressure homogenizer to carry out homogenization treatment to the Corynebacterium glutamicum liquid, homogenization 6 times under the condition of 70MPa;

[0038] (3) At 50°C, use sodium hydroxide solution to adjust the pH to 4.0, add protease and dextranase compound enzyme (addition ratio 3:1, addition amount 2%), inactivate the enzyme after hydrolysis for 10 hours, centrifuge, then its protein The recovery rate is 65.31%;

[0039] (4) The residue after enzymatic hydrolysis was acid-hydrolyzed at 115°C and pH 0.5. After hydrolysis for 18 hours, it was centrifuged, and the protein recovery rate was measured to be 80.02%;

[0040] (5) The recovery rate of total bacterial protein was 93% when treated with enzyme-acid binding method.

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Abstract

The invention relates to a method for preparing a glutamic acid fermentation organic nitrogen additive from glutamic acid fermentation waste thalli, which solves the problem that environment can be polluted by directly discharging fermented waste thalli or economic benefit is low when the fermented waste thalli are prepared into a feed additive in the conventional glutamic acid production. The glutamic acid fermentation waste thalli are taken as raw materials; protein hydrolyzate is obtained by enzymatic hydrolysis, acid hydrolysis or an enzymatic hydrolysis and acid hydrolysis combined method; and the hydrolyzate is concentrated or dried to prepare the organic nitrogen additive for fermentation. For the organic nitrogen additive prepared by the method, the protein recovery rate of the glutamic acid fermentation waste thalli reaches 93 percent; the prepared organic nitrogen additive can be recycled for glutamic acid fermentation and replaces yeast extract, peptone, corn steep liquor and other organic nitrogen additives which are used in the conventional glutamic acid production, so environmental pollution can be reduced and the added value of the organic nitrogen additive can be greatly improved; and the organic nitrogen additive has great significance for the comprehensive utilization of the glutamic acid fermentation waste thalli.

Description

【Technical field】 [0001] The invention belongs to the technical field of comprehensive utilization of monosodium glutamate fermentation industry, and in particular relates to a method for preparing glutamic acid fermentation organic nitrogen additives by using glutamic acid fermentation waste cells. 【technical background】 [0002] my country is a big producer of monosodium glutamate, which produces a large amount of monosodium glutamate every year. Generally, 1 ton of monosodium glutamate will produce 20 to 25 tons of high-concentration organic wastewater. The waste liquid contains 2% to 5% of glutamic acid fermentation waste cells, which are rich in Direct discharge of nutrients such as protein and nucleic acid will pollute the environment, and the economic benefit of being used as a feed additive after drying is also low. 【Content of invention】 [0003] The purpose of the present invention is to solve the problem that the direct discharge of organic waste water produced i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14C12R1/15
Inventor 肖冬光杜丽平郭学武周大伟张翠英陈叶福
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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