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Process for prepering gamma-polyglutamic acid and polyglutamates

A technology of polyglutamic acid and glutamic acid is applied in the field of preparation of γ-polyglutamic acid and its salts, which can solve the problems of low accumulation and achieve the effects of low dosage, light color and good moisture absorption and moisture retention.

Inactive Publication Date: 2002-05-01
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the accumulation of γ-polyglutamic acid in the culture medium is very low, and it is still difficult to produce γ-polyglutamic acid on a large scale and cheaply in the industry. Therefore, it is hoped to develop an efficient method for the preparation of γ-polyglutamic acid

Method used

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  • Process for prepering gamma-polyglutamic acid and polyglutamates
  • Process for prepering gamma-polyglutamic acid and polyglutamates

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Incline medium: 1-2% peptone, 0.5-1% beef extract, 0.5-1% NaCl, 2% agar, pH7.0

[0027] Shake flask medium: glucose 1.5%, yeast extract 1%, glutamic acid 2%, KH 2 PO 4 0.5%, MgSO 4 .7H 2 O0.05%, pH7.0. Fill 50ml of liquid in a 500ml Erlenmeyer flask, and sterilize at 121°C for 20 minutes.

[0028] Cultivate the purified Bacillus subtilis NX-2 on the slant medium at 25-37°C for 24-48h, then put a ring of the lawn in the shake flask medium, culture at 37°C for 48h, and shake the flask at a rotational speed of 180r / ml. Finally, the content of gamma-polyglutamic acid in the fermented liquid was 15 g / L.

Embodiment 2

[0030] The same culture conditions as in Example 1, using Bacillus licheniformis B-2 to obtain a γ-polyglutamic acid content of 10 g / L in the fermentation broth.

Embodiment 3

[0032] As in Example 1, the glucose in the shake flask culture medium was replaced with maltose, and the result was that the γ-polyglutamic acid content in the fermentation broth was 20 g / L.

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Abstract

A process for preparing gamma-polyglutamic acid and polyglutamate includes such steps as culturing bacillus, such as Bacillus subtilis and lichen bacillus, in the culture medium containing carbon source, nitrogen source and Glu to obtain high-activity (1-10 U / ml) gamma-glutamyl transpeptidase, preparing high-concentration fermented liquid of gamma-polyglutamic acid, and solvent precipitation or chemical precipitation.

Description

technical field [0001] The invention relates to a preparation method of gamma-polyglutamic acid and its salt. Background technique [0002] γ-polyglutamic acid (γ-PGA) and its salts are biodegradable polymer substances, which can be expected to be widely used in the fields of medicine, textiles, food, cosmetics, and agriculture. [0003] γ-polyglutamic acid can be formed by linking glutamic acid monomers through the traditional method of peptide synthesis. However, this method has no industrial application value, because the process of polymerizing glutamic acid needs to go through many complicated steps. [0004] On the other hand, as early as 1937, Ivanovics et al. discovered that γ-polyglutamic acid was a capsular component of Bacillus anthracis (Ivanovics G, Erdos Z. Immunt tsforsch, 1937, 90:5), and in 1942 Bovarnick et al. Began to study the use of microorganisms that can accumulate γ-polyglutamic acid in the medium to ferment and produce γ-polyglutamic acid (Bovarnic...

Claims

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Application Information

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IPC IPC(8): C08G69/02C12P13/14C12P21/02
Inventor 欧阳平凯徐虹
Owner NANJING UNIV OF TECH
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