The invention provides an enzymatic conversion preparation method of gamma-L-glutamoyl n-
propylamine. The method comprises the following steps: carrying out culture and
fermentation on a strain with the activity of gamma-glutamyl transpeptidase in a culture medium, and carrying out centrifugalizing on the
fermentation liquid, thus obtaining the wet
thallus containing gamma-glutamyl transpeptidase;(2) adding conversion liquid into the wet
thallus, carrying out enzymatic reaction under the condition with the temperature being 35-50 DEG C and the pH being 6-11, and carrying out separation by adopting an
isoelectric point crystallization method, thus obtaining gamma-L-glutamoyl n-
propylamine, wherein the conversion liquid contains L-
glutamic acid-gamma-methyl ester, n-
propylamine, and one ofethyl acetate,
butyl acetate,
octanol and n-
hexanol. According to the method, the specific strain of gamma-glutamyl transpeptidase is cultured in the culture medium, gamma-glutamyl transpeptidase is efficiently expressed, so that the
enzymatic synthesis of gamma-L-glutamoyl n-propylamine has relatively high
catalysis rate and conversion rate, in addition, the
reaction conditions are mild, the
stereoselectivity of
enzyme is strong, the
catalysis efficiency is high, the cost is low, the technical process is simple, and the method is suitable for industrial production.