Enzymatic conversion preparation method of gamma-L-glutamoyl n-propylamine

Abstract

The invention provides an enzymatic conversion preparation method of gamma-L-glutamoyl n-propylamine. The method comprises the following steps: carrying out culture and fermentation on a strain with the activity of gamma-glutamyl transpeptidase in a culture medium, and carrying out centrifugalizing on the fermentation liquid, thus obtaining the wet thallus containing gamma-glutamyl transpeptidase;(2) adding conversion liquid into the wet thallus, carrying out enzymatic reaction under the condition with the temperature being 35-50 DEG C and the pH being 6-11, and carrying out separation by adopting an isoelectric point crystallization method, thus obtaining gamma-L-glutamoyl n-propylamine, wherein the conversion liquid contains L-glutamic acid-gamma-methyl ester, n-propylamine, and one ofethyl acetate, butyl acetate, octanol and n-hexanol. According to the method, the specific strain of gamma-glutamyl transpeptidase is cultured in the culture medium, gamma-glutamyl transpeptidase is efficiently expressed, so that the enzymatic synthesis of gamma-L-glutamoyl n-propylamine has relatively high catalysis rate and conversion rate, in addition, the reaction conditions are mild, the stereoselectivity of enzyme is strong, the catalysis efficiency is high, the cost is low, the technical process is simple, and the method is suitable for industrial production.

Description

technical field

[0001] The invention relates to the field of preparation of γ-L-glutamyl n-propylamine, in particular to an enzymatic conversion preparation method of γ-L-glutamyl-n-propylamine. Background technique

[0002] γ-L-glutamyl n-propylamine is an important γ-glutamyl alkylamine, and γ-glutamyl alkylamine has important physiological activities. The γ-L-glutamyl n-propylamine can be used in the field of medicine and has a very wide application prospect.

[0003] γ-Glutamyl transpeptidase (EC 2.3.2.2) is an important enzyme involved in the glutamyl cycle reaction, which can specifically catalyze the migration process of glutamyl groups, so as to obtain compounds containing glutamyl groups. So far, there are reports on the synthesis of γ-D-glutamyl-L-tryptophan, glutathione, theanine, γ-glutamyl-taurine and other compounds catalyzed by γ-glutamyl transpeptidase There are many, but there is no report of using this enzyme to prepare γ-L-glutamine n-propylamine. [00...