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45 results about "Gamma-glutamyltransferase" patented technology
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Gamma-glutamyltransferase (also γ-glutamyltransferase, GGT, gamma-GT; EC 2.3.2.2) is a transferase (a type of enzyme) that catalyzes the transfer of gamma-glutamyl functional groups from molecules such as glutathione to an acceptor that may be an amino acid, a peptide or water (forming glutamate). GGT plays a key role in the gamma-glutamyl cycle, a pathway for the synthesis and degradation of glutathione as well as drug and xenobiotic detoxification. Other lines of evidence indicate that GGT can also exert a pro-oxidant role, with regulatory effects at various levels in cellular signal transduction and cellular pathophysiology. This transferase is found in many tissues, the most notable one being the liver, and has significance in medicine as a diagnostic marker.
Process for prepering gamma-polyglutamic acid and polyglutamates
A process for preparing gamma-polyglutamic acid and polyglutamate includes such steps as culturing bacillus, such as Bacillus subtilis and lichen bacillus, in the culture medium containing carbon source, nitrogen source and Glu to obtain high-activity (1-10 U / ml) gamma-glutamyl transpeptidase, preparing high-concentration fermented liquid of gamma-polyglutamic acid, and solvent precipitation or chemical precipitation.
Owner:NANJING UNIV OF TECH
Preparing gamma-poly glutamic acid, glutamate, glutathione and its precursor with bacillus subtilis NX-2
InactiveCN1425762AImprove protectionSimple process routeBacteriaFermentationProduction rateDipeptide
The bacillus subtilis NX-2, or CGMCC NO.0833 is one bacillus spawn obtained through screening and is used for the production of gamma-polyglutamic acid and its salt as well as glutathione and its precursor. In optimized condition, gamma-polyglutamic acid may be accumulated to 30-50 g / L and produced in the production efficinecy of 0.8-2.5 g / L. When bacillus subtilis NX-2 is used in fermenting to produce gamma-glutamyl transpeptidase in optimized condition, the fermented liquid may reach enzyme activity of 1-5 U / ml; and the fermented liquid or separation obtained enzyme or the immobilized enzyme is used in the production of glutathione and its precursor with substrate dipeptide or dipeptide precursor converting rate of 30-80% being reached.
Owner:WUHAN ELITE CHEM FERTILIZER
Preparation method for natural theanine
InactiveCN101343618AEfficient productionEasy to produceBacteriaTransferasesChinese traditionalTheanine
The invention relates to a natural theanine preparing method, which belongs to the technical field of the biologic engineering. Bacillus subtilis SK11.004 strain obtained through separating and selecting the Chinese traditional fermentation food is theanine producing strain of gamma-glutamyltransferase with the collection number CCTCC NO of M 208083; Gamma-glutamyl transpeptidase is acted on glutamine and ethylamine, and under the condition of pH 10, the theanine can be efficiently produced. The enzyme preparing method for theanine of the invention has the advantages that the reaction time is short, the preparation of the enzyme activity source is simple, the production of the theanine is ensured to be simple, and the product is safe and reliable. The preparing method is suitable for performing large-scale industrialized production.
Owner:JIANGNAN UNIV
Method for producing gamma-glutamyl transpeptidase using bacillus subtilis
InactiveCN101363014AReduce the cost of fermentation productionBacteriaTransferasesGamma-glutamyl L-dopaTryptophan
A method for producing Gamma-glutamyltranspeptidase by using Bacillus subtilis belongs to the biotechnology field of foods. The method carries out the three-stage culture of the slope, the seeds and the fermentation of the producing bacteria Bacillus subtilis SK11.004 of the Gamma-glutamyltranspeptidase; and the Gamma-glutamyltranspeptidase powder is obtained by carrying out separation and purification of fermentation broth. The provided method for producing the Gamma-glutamyltranspeptidase by using the Bacillus subtilis SK 11.004 has small breeding workload, low cost and safe and reliable performances, the method can carry out the liquid fermentation production, the cost is low, the method can be used in large-scale production, and the enzyme activity of the Gamma-glutamyltranspeptidase which is produced by using the Bacillus subtilis can be up to more than 4U / mL. The fermented product is non-toxic and can be directly used for producing a plurality of valuable Gamma-glutamyl compounds, such as L-Gamma-glutamyl-L-dopa, Gamma-glutamyl taurine, Gamma-glutamyl tryptophan, glutathione, theanine, and the like.
Owner:JIANGNAN UNIV
Fluorescent probe for detecting gamma-glutamyl transpeptidase as well as preparation method and application of fluorescent probe
InactiveCN104892541ARealize determinationVisibly pinkish purpleOrganic chemistryFluorescence/phosphorescenceOrganic solventFluorescence
Owner:INST OF CHEM CHINESE ACAD OF SCI
Immobilized enzyme using modified epoxy resin as carrier and preparation method and application thereof
InactiveCN102559648AUniform shapeImprove stabilityChemical industryOn/in organic carrierHydrogenGamma-glutamyltransferase
The invention discloses an immobilized enzyme using modified epoxy resin as a carrier, which comprises an enzyme and the modified carrier for fixing the enzyme. The enzyme is gamma-glutamyl transpeptidase, and the modified carrier is the epoxy resin Eupergit C250L modified through carrier ampholyte with potential of hydrogen (pH) 8-10.5. The invention further discloses a preparation method and application of the immobilized enzyme. The immobilized enzyme has the advantages of being uniform in shape, strong in enzyme stability, good in repeated use performance, strong in reaction environment pH adaptability and the like. Stability of the gamma-glutamyl transpeptidase is improved further, and application of the novel immobilized enzyme in synthesis of theanine is achieved.
Owner:NANJING UNIV OF TECH
Dietary modulators of gamma glutamyl transpeptidase
InactiveUS20010034325A1Easy to solveEasy to adjustSenses disorderNervous disorderGamma-glutamyltransferaseBiology
The invention relates to the new compositions comprising probiotic glucoproteins and isolated soy proteins for lowering upregulated gamma glutamyl transpeptidase humans or domestic animals. Their synergetic effects with N-acetyl glucosamine is disclosed.
Owner:SLESAREV VLADIMIR I
Application of Chinese herb Pien Tze Huang and preparation thereof in preparation of drugs for treating non-alcoholic fatty liver disease (NAFLD)
InactiveCN104042655AImprove liver functionDecreased liver functionDigestive systemMammal material medical ingredientsSerum igeLiver function
The invention belongs to the field of pharmacology of Chinese medicines and discloses an application of a Chinese herb Pien Tze Huang and a preparation thereof in preparation of drugs for treating non-alcoholic fatty liver disease (NAFLD). The Chinese herb Pien Tze Huang can be used for remarkably improving the liver function of a fatty liver rat, reducing the AST (Aspartate Aminotransferase), TG (Triglyceride) and (gamma-Glutamyl Transpeptidase) level of serum, reducing the degree of fatty liver and relieving inflammatory reaction and has a remarkable curative effect on the aspect of treating NAFLD.
Owner:ZHANGZHOU PIEN TZE HUANG PHARM
Co-culture system for liver cells and Kupffer cells and application thereof
InactiveCN102220281ADoes not affect proliferationRapid cultivationMicrobiological testing/measurementArtificial cell constructsSerum glutamate pyruvate transaminaseFactor ii
The invention discloses a co-culture system for liver cells and Kupffer cells and application thereof. The co-culture system is characterized in that in a Millicell double-layer culture room, 1-100 ng / mL LPS (lipopolysaccharides) is added into a culture medium, the Kuffer cells are cultured in the upper layer of the Millicell double-layer culture room, and the liver cells are cultured in the lower layer of the Millicell double-layer culture room; and half of the fresh culture medium is replaced every other day so as to keep the concentration of the LPS. The co-culture system for liver cells and Kupffer cells provided by the invention has the advantages that the Kupffer cells continuously secrete inflammatory cell factors (such as TNF-alpha) in vitro after being stimulated by the LPS; the liver cells can generate a great number of enzymes, such as AST (aspartate aminotransferase), ALT (alanine transaminase) and GGT (gamma-glutamyl transpeptidase) in the environment so as to stimulate the liver injury based on immunoreaction caused by the immune / inflammation response after the liver tissue undergoes virus infection in vivo; and moreover, the co-culture system is used for screening medicaments resisting immune liver injury according to the influence of the medicaments on the secretion amount of immune factors and the enzymatic activity.
Owner:SHAANXI INST FOR FOOD & DRUG CONTROL
Gamma-glutamyl transpeptidase inhibitors and methods of use
InactiveUS20100197745A1High sensitivityGood effectBiocideAnimal repellantsTherapy resistantCancer cell
The present invention enhances the effects of antitumor agents and increases the sensitivity of therapy-resistant tumor cells to antitumor agents such as chemotherapeutic agents and radiation. The present invention is thus directed to compositions and methods for inhibiting and killing neoplastic cancer cells, for example for the treatment, inhibition or prevention of tumors or malignant growths or other neoplasias in mammals. The GGT inhibitor compounds used in the methods of the present invention comprise a class of benzylthiadiazol benzenesulfoniamides represented by the general structure Formula (I) (FIG. 1), or a pharmaceutically acceptable salt thereof, wherein any one or more of R1-R10 may be H, Cl, F, Br, I, OH, an alkoxy, or NO2. Other R groups include carrier groups linked by C,N, or O. The present invention also provides a method for the prophylaxis or treatment of a reversible airways obstruction in a mammal, such as a human, comprising administration of a therapeutically effective amount of a GGT inhibitor described herein for the prophylaxis or treatment of a disease associated with reverse airways obstruction such as asthma, chronic obstructive pulmonary disease (COPD), allergic reaction, respiratory tract infection or upper respiratory tract disease. Other diseases or conditions which may be treated include, for example, degenerative diseases, renal diseases, liver diseases, and inner ear conditions or diseases.
Owner:THE BOARD OF RGT UNIV OF OKLAHOMA
Control technical system for important metabolic disease of dairy cattle after delivery
The present invention relates to a control technical system for important metabolic disease of dairy cattle after delivery, belonging to the technical field of veterinary medicine. The technical system is used for controlling and preventing important metabolic diseases of dairy cattle after delivery by detecting dairy cattle before and after delivery in the aspects of dietary cation anion balance (DCAB), oxidation resistance capability of cow plasma (glutathione peroxidase GSH-Px, superoxide dismutase SOD, methylene dioxyamphetamine MDA), plasma leptin hormone levels, liver function indexes (ornithine carbamoyl transferase OCT, aspartate transaminease AST, gamma-glutamyl transpeptidase GGT, total bilirubin Tbil, total protein TP, albumin ALB), blood biochemical indexes (acetone bodies, nonesterified fatty acid NEFA, lactic acid, blood sugar, blood fat, triglyceride TG, total cholesterol TC, calcium, phosphorus and magnesium), cow appetite, milk output increase speed, etc. The important metabolic diseases include cow parturient paresis, retention of afterbirth, endometritis, ketosis, ruminant acidosis, mastitis and fatty liver. The present invention is suitable for preventing and controlling metabolic diseases of dairy cattle after delivery in large-scale dairy farms.
Owner:何宝祥 +2
MALDI-TOF mass spectrometric probe for gamma-glutamyl transpeptidase detection and use method thereof
ActiveCN108424436AEasy to synthesizeStable under mass spectrometry conditionsMicrobiological testing/measurementBiological material analysisLysosomeStructural formula
The invention relates to an MALDI-TOF mass spectrometric probe for gamma-glutamyl transpeptidase detection and a use method thereof. The structural formula of the probe is shown as the specification.The probe is used for quantitative analysis of GGT enzyme and screening of related inhibitor drugs, compared with the prior art, the molecular probe synthesis cost and labor force are reduced, the interference of substrate light signal, light scattering, photobleaching and other phenomena in the spectrum can be avoided, rapid and accurate analysis of GGT enzyme in cell lysosomes and human serum can be achieved, simultaneous analysis of hundreds of samples on an MALDI target plate can be realized, and the analysis speed and efficiency are greatly improved.
Owner:JILIN UNIV
Preparation method of sulfur-allyl-L-cysteine
InactiveCN103451249AAvoid the problem of oxidase oxidation to alliinEasy to makeFermentationEvaporationOxidative enzyme
The invention discloses a preparation method of sulfur-allyl-L-cysteine. The preparation method comprises the following steps of dissolving gamma-glutamoyl-sulfur-allyl-L-cysteine in a buffer solution, and adding gamma-glutamyl transpeptidase as a catalyst to the buffer solution for reaction so as to obtain a reaction liquid containing the sulfur-allyl-L-cysteine; sending the reaction liquid to a chromatographic column for adsorption separation, washing with de-ionized water and eluting by using a 0.3 mol / L-1.2 mol / L ammonia solution, and collecting an eluant containing the sulfur-allyl-L-cysteine; adding a solvent after the eluant is subjected to evaporation and concentration, stewing at a room temperature and separating out crystals; washing the crystals with ethanol, and recrystallizing by using an ethanol solution with the volume fraction of 60% after the crystals are dried so as to obtain the crystals containing the sulfur-allyl-L-cysteine. The preparation method of the sulfur-allyl-L-cysteine has the advantages that preparing the sulfur-allyl-L-cysteine is prepared in a high yield and the problem that the sulfur-allyl-L-cysteine is oxidized into alliin by oxidase in garlic cells is avoided.
Owner:SICHUAN XICHENG NATURAL FOODS
Strong-taste peptide, strong-taste endowing agent, seasoning and preparation method of strong-taste peptide
InactiveCN107114752AHas savory propertiesPeptides with abnormal peptide linkFood scienceGlutaminaseValine
The invention discloses strong-taste peptide, a strong-taste endowing agent, seasoning and a preparation method of the strong-taste peptide. The strong-taste peptide (gamma-Glu-gamma-Glu-gamma-Glu-gamma-Glu-val) disclosed by the invention has favorable stability, and can significantly improve the strong taste of foods particularly condiments under the concentration of 20-100mg / kg. The preparation method comprises the specific preparation steps of mixing glutamine with valine to obtain a mixture, dissolving the mixture in water to obtain a solution, adding glutaminase of which the mass is 0.001-0.4%(w / w) of that of the solution or gamma-glutamyl transpeptidase of which the mass is 0.001-0.4%(w / w) of that of the solution, regulating the pH of the solution to be 8.0-10.0 with a 10mol / l sodium hydroxide solution, performing a reaction at 25-50 DEG C for 3-12 hours, then regulating the pH of the solution back to be 6-7 with 4 mol / l hydrochloric acid, and performing enzyme deactivation at 90 DEG C for 10min.
Owner:SOUTH CHINA UNIV OF TECH
Multifunctional lactobacillus buchneri CCFM1053 for relieving PFOA toxic action as well as fermented food and application thereof
The invention discloses multifunctional lactobacillus buchneri CCFM1053 for relieving PFOA toxic action as well as fermented food and application thereof. The lactobacillus buchneri CCFM1053 can adsorb PFOA, improve spleen atrophy caused by PFOA exposure, increase content of TNA-alpha in serum after PFOA exposure, increase content of glutamic-pyruvic transaminase, glutamic oxalacetic transaminaseand gamma-glutamyl transpeptidase in serum in mice after PFOA exposure, reduce the content of MDA in liver after PFOA exposure to the normal level, reduce GAH activity, improve intestinal flora disorder, reduce hepatopathy, improve excrement moisture content and first melena discharge time of constipation patients, relieve constipation, improve proliferation of INS-1 cells and expression of MafA genes under the high glucose action and relieve PFOA related diabetes mellitus.
Owner:JIANGNAN UNIV
Chemical enzymatic process of synthesizing glutathion
InactiveCN1772916APromote conversionImprove bindingTransferasesFermentationBenzyl groupGamma-glutamyltransferase
The present invention discloses chemical enzymatic process of synthesizing glutathion. The chemical enzymatic process includes the following steps: A. synthesizing substrate chemically; B. preparing gamma-glutamyl transpeptidase; and C. adding gamma-glutamyl transpeptidase into the substrate solution to produce transpeptidase reaction preparing glutathion. The said substrate is S-benzyl-cysteinyl glycine and L-glutamine. The process is simple, low in cost and high in glutathion converting rate, and may be used in industrial production.
Owner:NANJING UNIV OF TECH
Method for modifying gamma-glutamyl transpeptidase immobilized enzyme by using carrier ampholyte
InactiveCN103937776AIncrease loading capacityImproves pH stabilityOn/in inorganic carrierSolventHigh activity
The invention discloses a method for modifying gamma-glutamyl transpeptidase immobilized enzyme by using carrier ampholyte. The method comprises the following steps: in the environment of a toluene solvent, chemically modifying the surface of mesoporous titanium oxide by 3-aminopropyl triethoxysilane to obtain modified mesoporous titanium dioxide; mixing a gamma-glutamyl transpeptidase liquid with mesoporous titanium dioxide to obtain a mixture and oscillating the mixture to obtain immobilized enzyme; mixing the immobilized enzyme with Pharmalyte (CA) and oscillating for reaction; and filtering out raffinate, washing with a buffer solution and performing filtering-drying. According to the method, gamma-glutamyl transpeptidase (GGT) is immobilized with high-specific-surface-area mesoporous titanium oxide as a carrier and the carrying capacity of the carrier is high; the immobilized enzyme is modified by carrier pharmalyte with isoelectric point approximating the optimum pH of GGT; the pH tolerance of the immobilized gamma-glutamyl transpeptidase is improved effectively simultaneously when the stability of the immobilized enzyme is enhanced, and high activity is maintained in the pH range of 6-11.
Owner:NANJING UNIV OF TECH
Supplementary strategy for improving catalytic synthesis of L-theanine from gamma-glutamyl transpeptidase
InactiveCN104789538AAvoid inhibitionImprove conversion rateMicroorganism based processesAcyltransferasesL-theanineL-Glutamin
The invention discloses to further optimization of a process for generating L-theanine from L-glutamine and ethylamine catalyzed by gamma-glutamyl transpeptidase secreted from recombinant pMA5-ggt / Bacillus. subtilis by using a method that double substrates are added in different batches, and belongs to the field of fermentation engineering and enzyme engineering. The process comprises the following steps: culturing the strain of bacillus subtilis which is established in previous operation and is used for secreting gamma-glutamyl transpeptidase in a fermentation tank; concentrating supernate crude enzyme liquid for conversion of theanine; on the basis, adding the substrates in different batches so as to further regulate and control the conversion process, that is, in a reaction system with 60U / mL of the enzyme liquid, adding substrates glutamine and ethylamine into the system every other two hours, wherein the addition amount of glutamine is fixed to be 20 mM, the ratio of a donor to a receptor is controlled to be 1:12, the pH value is adjusted to be 10, 164.2 mM of theanine can be obtained after 18 hours, and the conversion rate of glutamine can be up to 91.2%. The method has the advantages that the operation is simple, the cost is low, the yield of theanine is high, the substrate conversion rate is high, and the like, and is beneficial for industrial large-scale production.
Owner:JIANGNAN UNIV
Process for the enzymatic preparation of a gamma-glutamyl compound
Disclosed is a process for the enzymatic preparation of a gamma-glutamyl compound. The process comprises a step of contacting a gamma-glutamyl donor and a gamma-glutamyl acceptor with an aqueous medium comprising a gamma glutamyl transpeptidase enzyme. The enzyme is derived from a plant belonging to the Graminaceae or Leguminaceae family, or from Camellia sinensis.
Owner:CONOPCO INC D B A UNILEVER
Preparing gamma-poly glutamic acid, glutamate, glutathione and its precursor with bacillus subtilis NX-2
InactiveCN1164737CImprove protectionSimple process routeBacteriaFermentationProduction rateDipeptide
The bacillus subtilis NX-2, or CGMCC NO.0833 is one bacillus spawn obtained through screening and is used for the production of gamma-polyglutamic acid and its salt as well as glutathione and its precursor. In optimized condition, gamma-polyglutamic acid may be accumulated to 30-50 g / L and produced in the production efficinecy of 0.8-2.5 g / L. When bacillus subtilis NX-2 is used in fermenting to produce gamma-glutamyl transpeptidase in optimized condition, the fermented liquid may reach enzyme activity of 1-5 U / ml; and the fermented liquid or separation obtained enzyme or the immobilized enzyme is used in the production of glutathione and its precursor with substrate dipeptide or dipeptide precursor converting rate of 30-80% being reached.
Owner:WUHAN ELITE CHEM FERTILIZER
Compositions and methods that modulate phosphorus or enzymes in a companion animal
PendingUS20180168194A1Low costNo reduction in nutritional benefitsNervous disorderHydroxy compound active ingredientsPhysiologyAspartate Aminotransferases
A specific type of organ health marker in a companion animal can be improved by adjusting the diet of the animal to increase the amount of a compound which positively or negatively modulates the specific type of organ health marker or adjusting the diet of the animal to decrease the amount of a compound which positively or negatively modulates the specific type of organ health marker. The specific type of organ health marker can be one or more of phosphorus, alkaline phosphatase, aspartate aminotransferase, or gamma-glutamyltransferase.
Owner:SOC DES PROD NESTLE SA
Gamma glutamyl transpeptidase inhibitors and methods of use
ActiveUS8741937B2High sensitivityGood effectBiocideOrganic chemistryTranspeptidase InhibitorsCancer cell
Compositions and methods for inhibiting human gamma-glutamyl transpeptidase (GGT) in vivo or in vitro and for inhibiting and killing neoplastic cancer cells, for example for the treatment, inhibition or prevention of tumors or malignant growths or other neoplasias in mammals, or sensitizing tumor to other therapies including radiation therapy. The GGT inhibitor compounds comprise a class of benzylthiadiazol benzenesulfoniamides. The compounds may also be used to treat a reversible airways obstruction in a mammal or a disease associated with reverse airways obstruction such as asthma, chronic obstructive pulmonary disease (COPD), allergic reaction, respiratory tract infection or upper respiratory tract disease. Other diseases or conditions which may be treated include, for example, degenerative diseases, renal diseases, liver diseases, cardiovascular disease and inner ear conditions or diseases.
Owner:THE BOARD OF RGT UNIV OF OKLAHOMA
Strong-taste peptide, strong-taste endowing agent, seasoning and preparation method of strong-taste peptide
The invention discloses strong-taste peptide, a strong-taste endowing agent, seasoning and a preparation method of the strong-taste peptide. The strong-taste peptide (gamma-Glu-gamma-Glu-gamma-Glu-gamma-Glu-val) disclosed by the invention has favorable stability, and can significantly improve the strong taste of foods particularly condiments under the concentration of 20-100mg / kg. The preparation method comprises the specific preparation steps of mixing glutamine with valine to obtain a mixture, dissolving the mixture in water to obtain a solution, adding glutaminase of which the mass is 0.001-0.4%(w / w) of that of the solution or gamma-glutamyl transpeptidase of which the mass is 0.001-0.4%(w / w) of that of the solution, regulating the pH of the solution to be 8.0-10.0 with a 10mol / l sodium hydroxide solution, performing a reaction at 25-50 DEG C for 3-12 hours, then regulating the pH of the solution back to be 6-7 with 4 mol / l hydrochloric acid, and performing enzyme deactivation at 90 DEG C for 10min.
Owner:SOUTH CHINA UNIV OF TECH
Sweet taste peptide as well as preparation method and application thereof
InactiveCN108794573AHas sweet peptide propertiesImprove stabilityPeptide preparation methodsFermentationSucroseGlutaminase
The invention discloses a sweet taste peptide as well as a preparation method and application thereof. The preparation method specifically comprises the steps as follows: mixing L-glutamine with D-valine and dissolving in water, adding glutaminase or gamma-glutamyl transpeptidase with the mass being 0.001-0.4% (w / w) of the solution mass, adjusting the pH of the solution to 8.0-10.0 with a 10 mol / lsodium hydroxide solution, allowing a reaction at 25-50 DEG C for 3-12 h, then adjusting the pH back to 6-7 with 4 mol / L hydrochloric acid, and performing enzyme denaturing at 90 DEG C for 10 min. The gamma-glutamine peptide (gamma-L-Glu-gamma-L-Glu-gamma-L-Glu-gamma-L-Glu-D-Val) prepared by the invention is good in stability and has a natural sweet taste similar to that of sucrose.
Owner:GUANGDONG POLYTECHNIC OF ENVIRONMENTAL PROTECTION ENG
Application of monomeric compounds of fruits of Chinese magnoliavine (Schisandra chinensis(Turcz.) baill. and Schisandra sphenanthera Rehd.et Wils) to preparation of medicine for preventing and treating cholestasis liver diseases
InactiveCN107569481APrevention and Treatment of Congestive Liver InjuryProve the effect of the treatmentDigestive systemEther/acetal active ingredientsAlanine aminotransferaseHepatica
The invention discloses application of monomeric compounds of fruits of Chinese magnoliavine (Schisandra chinensis(Turcz.) baill. and Schisandra sphenanthera Rehd.et Wils) to preparation of medicine for preventing and treating cholestasis liver diseases. The monomeric compounds of fruits of Chinese magnoliavine refer to Schisandrin B, Schisandrin C, Schisandrol A, Schisantherrin A and Schisantherrin B. The result of the study shows that all of the five kinds of monomeric compounds can obviously reduce the content of total bile acids and direct bilirubin in blood serum in the cholestasis liverdisease state, can relieve the cholestasis symptoms, can reduce the activity of alkaline phosphatase, gamma-glutamyltransferase, aspartate transaminase and / or alanine aminotransferase in the liver, and can effectively improve the liver tissue necrosis degree. The invention discovers the condition for the first time that the five kinds of monomeric compounds of fruits of Chinese magnoliavine can beused for effectively preventing and treating the cholestasis liver diseases induced by liithocholic acid. The discovery provides the powerful technical theoretical basis for the new medicine researchand development for the cholestasis liver diseases and the clinic application of the monomeric compounds of fruits of Chinese magnoliavine; and wide application prospects are realized in the field ofcholestasis prevention and treatment.
Owner:SUN YAT SEN UNIV
AD biomarker and detection method thereof
ActiveCN106645755AAccurately reflect the pathological development statusReflect the pathological developmentDisease diagnosisBiological testingPolymeric immunoglobulin receptorTubulin Alpha
The invention discloses an AD biomarker and a detection method thereof. The AD biomarker comprises at least one of Protein-glutamine gamma-glutamyltransferase E, Protein S100-A12, Alph-2-macroglobulin-like protein 1, Polymeric immunoglobulin receptor, Myeloperoxidase, Peroxiredoxin-1, Protein S100-A11, Alpha-2-macroglobulin, Cystatin-C, Histone H2A, Histone H2B, Tubulin alpha-1B and Annexin A3.
Owner:SHENZHEN UNIV
Fluorescent probe for detecting high-concentration gamma-Glutamyl Transpeptidase and preparation method thereof
ActiveCN108893106AGood luminous propertiesThe synthesis process is simplePeptide preparation methodsGroup 3/13 element organic compoundsHigh concentrationFluorescence
The invention provides a fluorescent probe for detecting high-concentration gamma-Glutamyl Transpeptidase, which solves the problem of selective response to abnormally high-concentration gamma-Glutamyl Transpeptidase in vivo. The fluorescent probe of the invention starts to produce obvious fluorescence changes after the gamma-Glutamyl Transpeptidase is higher than the normal concentration of humanbody, and as the concentration of gamma-Glutamyl Transpeptidase increases, the fluorescent probe solution of the invention produces large fluorescence enhancement and obvious color change. At the same time, after the fluorescent probe of the invention reacts with gamma-Glutamyl Transpeptidase, the permeability of the cell membrane is greatly enhanced, and when applied to cell imaging, the fluorescent probe can selectively image ovarian cancer cells with high expression of gamma-Glutamyl Transpeptidase. According to the invention, the synthesis process is simple and feasible, the raw materialsare cheap and easy to obtain, the preparation cost is low, and the preparation method is easy to popularize.
Owner:川北医学院
Method for preparing theanine by using species coupling ATP regenerative technology
InactiveCN101457241BConducive to conversion productionIncrease production capacityMicroorganism based processesFermentationMicroorganismMicrobial transformation
The invention discloses a method for preparing a theanine by an interspecific coupling ATP regenerative technology comprising the following steps: preparing gamma-glutamyltransferase immobilized cells by using bacillus licheniformis, preparing 1,6-diphosphate conversion culture solution by using bacillus stearothermophilus; preparing the theanine conversion solution by mixing the gamma-glutamyltransferase immobilized cells, 1,6-diphosphate conversion culture solution and substrates which are glutamine (glutamic acid) and ethylamine; obtaining the theanine by extracting and refining the theanine conversion liquid. The technical scheme provided by the invention is a fine new technical scheme for theanine preparation by coupling the ATP regenerative technology and an enzyme conversion technology, a technology capable of promoting the ATP consumed enzyme reaction smooth, also a technology suitable for the theanine production by the microorganism conversion method and has a wide application prospect in the food, medical treatment and the enzyme engineering field.
Owner:GUANGDONG PHARMA UNIV
A compound, a product containing the compound and applications in detection of gamma-glutamyl transpeptidase
ActiveCN110204476AHigh sensitivityReduce distractionsGroup 5/15 element organic compoundsColor/spectral properties measurementsGamma-glutamyltransferaseOrganic chemistry
Owner:FUDAN UNIV +1
Gamma-glutamyl transpeptidase inhibitors and methods of use
ActiveUS20130085168A1High sensitivityGood effectBiocideOrganic chemistryCancer cellTranspeptidase Inhibitors
Compositions and methods for inhibiting human gamma-glutamyl transpeptidase (GGT) in vivo or in vitro and for inhibiting and killing neoplastic cancer cells, for example for the treatment, inhibition or prevention of tumors or malignant growths or other neoplasias in mammals, or sensitizing tumor to other therapies including radiation therapy. The GGT inhibitor compounds comprise a class of benzylthiadiazol benzenesulfoniamides. The compounds may also be used to treat a reversible airways obstruction in a mammal or a disease associated with reverse airways obstruction such as asthma, chronic obstructive pulmonary disease (COPD), allergic reaction, respiratory tract infection or upper respiratory tract disease. Other diseases or conditions which may be treated include, for example, degenerative diseases, renal diseases, liver diseases, cardiovascular disease and inner ear conditions or diseases.
Owner:THE BOARD OF RGT UNIV OF OKLAHOMA
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