Sweet taste peptide as well as preparation method and application thereof
A technology of sweetness and peptide γ, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of differences in taste characteristics, little mention of taste characteristics, and little research on D-amino acids. achieve good stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] Dissolve 300mM glutamine and 300mM valine in 100g water, adjust the pH value of the solution to 10.0 with 10 mol / l sodium hydroxide solution, add 0.4g glutaminase (Japan Amano Co., Ltd., the activity is 100GTU / g) React at 37°C for 12 hours, then adjust the pH to 7.0 with 4 mol / L hydrochloric acid, inactivate the enzyme at 90°C for 10 minutes, and perform sensory evaluation on the taste characteristics of the product. The taste characteristics before and after the reaction are as follows: figure 1 .
[0025] Depend on figure 1 It can be seen that after the reaction, the sweetness and umami taste of the reaction solution are significantly improved. It shows that the substance of the reaction solution has changed, and a substance with sweet taste is produced. In order to further confirm the change of substances, the reaction solution was used for high performance liquid chromatography to determine the product.
[0026] Quantitative analysis of HPLC liquid relative to t...
Embodiment 2
[0032] Add 0.01g of purified γ-L-Glu-γ-L-Glu-γ-L-Glu-γ-L-Glu-D-Val to the black coffee solution or black tea containing an appropriate amount of sugar-free milk powder to test the product Sensory evaluation. The sensory evaluation experiment consisted of 7 males and 9 females aged 30-41 with sensory evaluation experience. The sensory evaluation results are shown in Figure 5 a and b. Depend on Figure 5a It can be seen that when this sweet peptide is added to black coffee containing milk powder, compared with the blank group, the sweetness of the solution is significantly increased, and the bitterness and sourness are significantly reduced, which shows that this sweet peptide produces coffee mates similar to sucrose. The effect is to give black coffee a sweet taste. Such as Figure 5b , After adding sweet peptides, a taste similar to milk tea appeared, and the sweetness and silky feeling increased. Judging from these two applications, this sweet peptide can be used to repla...
Embodiment 3
[0034]Inoculate Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) SWJS22 (General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation number CGMCC No.8425) with 1% inoculum in the fermentation medium (the composition of the fermentation medium is: 4 % soybean meal, 2% bran, 2% corn flour), fermented at 37~39°C for 30h. The fermentation broth was centrifuged at 4°C and 10,000 r / min at high speed for 10 minutes, and the supernatant was filtered to obtain the crude glutaminase enzyme solution.
[0035] Dissolve 600mM glutamine and 300mM valine in 100g water, adjust the pH of the solution to 9.0 respectively, add 1g of crude enzyme solution, react at 40°C for 12h, adjust the pH to 7.0 with 4 mol / L hydrochloric acid, Inactivate the enzyme at 90°C for 10 min to obtain a reaction solution of γ-L-Glu-γ-L-Glu-γ-L-Glu-γ-L-Glu-D-Val. Using XSelect HSS T3 5μm4.6x 250mm analytical column, mobile phase A is 0.1% (V / V) formic acid-water s...
PUM
Property | Measurement | Unit |
---|---|---|
Concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com