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Amino acid fermentation bacteria utilization method

A technology of glutamic acid fermenting bacteria and cells, which is applied in the field of preparing enzymatic protein paste and protein powder to achieve the effects of convenient operation, easy enzymatic hydrolysis and improved product quality

Active Publication Date: 2012-10-10
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, it is an urgent technical problem in this field to study a systematic enzymatic hydrolysis and utilization of glutamic acid bacterium proteolysis to reduce wastewater pollution and reduce the nutritional value of bacterium protein due to the introduction of flocculants.

Method used

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  • Amino acid fermentation bacteria utilization method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Firstly, 10 cubic meters of glutamic acid fermentation broth is separated from the fermentation cells by a disc separator. Liquid and thalline protein were separated to obtain thalline protein 6500kg.

[0022] (2) Add the above bacterial protein into the stirring reactor, add an appropriate amount of warm water and mix thoroughly, adjust the solid content to 8%, adjust the enzymolysis reaction temperature to 55°C, add a little sulfuric acid to adjust the pH to 6.5, and add lysozyme 10kg / m 3 , acid protease 25Kg / m 3 , β-glucanase 5Kg / m 3 , slowly stirring the enzymolysis time for 6 hours, and using an amino acid analyzer to measure the contents of various amino acids in the enzymolysis solution.

[0023] (3) The above-mentioned enzymatic hydrolysis solution is separated and removed by a disc separator to remove the cell wall, and the obtained supernatant is evaporated at a low temperature by a three-effect plate evaporator. The first-effect feed temperature of the ...

Embodiment 2

[0026] The enzymolyzed bacterial protein paste obtained in Example 1 was dried in a spray granulation fluidized bed, and the obtained finished product was packaged into enzymatic bacterial protein powder, and the temperature of the hot air in the spray granulation fluidized bed was 130°C.

Embodiment 3

[0028] Using the enzymolyzed protein paste obtained in Example 1 as a nitrogen source to replace commercially available yeast powder, a threonine fermentation test was carried out, and the average fermentation parameters of 5 consecutive tanks were respectively:

[0029] Using the enzymolyzed protein paste prepared in Example 1 as a nitrogen source, the fermentation broth produced 13% acid, the fermentation period was 28 hours, and the fermentation sugar-acid conversion rate was 68%.

[0030] Using commercially available yeast powder as a nitrogen source, the fermentation broth produced 14% acid, the fermentation period was 28 hours, and the fermentation sugar-acid conversion rate was 67%.

[0031] Through the above comparison, the product produced by the present invention can completely replace commercially available yeast powder products for threonine fermentation, thereby greatly reducing the cost of threonine fermentation.

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Abstract

The invention discloses an amino acid fermentation bacteria utilization method, which belongs to the technical field of amino acid production. The method comprises the steps that by taking glutamic acid fermentation as an example and adopting the isoelectric point of fermentation broth, a disc separator is utilized to separate mycoprotein in the fermentation broth, and a moderate amount of compound enzyme is added into the mycoprotein serum, so that the walls of bacteria are broken and the bacteria are enzymetically hydrolyzed. The enzymatic hydrolysate is separated by the high-speed disc separator, and the cell walls are removed, the obtained clarified enzymatic hydrolysate is concentrated under low temperature to produce the enzymetically hydrolyzed mycoprotein extract, or the clarified enzymatic hydrolysate is dried by a gunite granulation fluidized bed drier, a spray granulation drier and the like to produce the enzymetically hydrolyzed mycoprotein powder. In the method, the utilization value of the mycoprotein is greatly increased, so that the mycoprotein is changed from cheap feed into high value-added yeast extract and yeast powder substitute, the product can be used as fermentation ingredient and in other fields, the resources can be sufficiently utilized in the whole process, the purposes of energy saving and consumption reduction are achieved, and the invention has a broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of amino acid production, and relates to a method for utilizing amino acid fermentation cells, in particular to a method for enzymatically hydrolyzing glutamic acid fermentation cells with a compound enzyme preparation to prepare enzymolyzed protein paste and protein powder. Background technique [0002] Glutamic acid thallus is a by-product in the process of producing monosodium glutamate by fermentation. It is a waste thallus after extracting and refining monosodium glutamate produced by glutamic acid-producing bacteria. It is a single-cell protein, rich in protein, and has a After the analysis of the chemical composition of the cell protein, it was found that the protein content in the glutamic acid cell was as high as 85.8%, and the total amino acid content was 78.77%, which was higher than that of soybean meal and yeast, which are commonly used as raw materials for protein hydrolyzate. Its amino acid ty...

Claims

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Application Information

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IPC IPC(8): C12P21/06A23K1/16
Inventor 唐永强冯珍泉汲广习马仕敏徐进钊李树标董吉子
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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