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Method for treating threonine mother liquor

A treatment method and technology of threonine mother, applied in the field of threonine production, can solve the problems of low pollution and high energy efficiency, and achieve the effects of simple process, reduced investment, and avoidance of potential feed safety hazards.

Active Publication Date: 2014-03-12
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of the present invention not only solves the problem of separating effective components in threonine mother liquor, but also enables full utilization of threonine fermentation cell protein and residual sugar, and has high energy efficiency, less waste water discharge, low pollution, and electrodialysis technology It has the advantages of simple operation, no pollution, and long service life. It not only reduces the investment cost of enterprises, but also saves energy and reduces emissions. It has multiple effects.

Method used

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  • Method for treating threonine mother liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for processing threonine mother liquor, comprising the steps of:

[0021] Centrifugal separation: Use a high-speed disc separator to separate the bacterial protein in the threonine mother liquor. The speed of the high-speed disc separator is about 4000-5000r / min, recover the bacterial protein precipitate, and collect the supernatant.

[0022] Membrane filtration for secondary removal of bacterial protein: filter the supernatant obtained in step 1) with a ceramic membrane with a membrane pore size of 40nm, wherein the filtration temperature is 37°C, the membrane inlet pressure is 2.0bar, the bacterial protein is recovered by filtration, and collected filtrate.

[0023] Cellular protein treatment: Combine the bacterial protein in step 1) and step 2) into the stirring reactor, add an appropriate amount of warm water and mix thoroughly, adjust the solid content to 8%, adjust the enzymolysis reaction temperature to 55°C, add a little sulfuric acid to adjust the pH t...

Embodiment 2

[0026] Using the enzymolyzed protein paste obtained in Example 1 as a nitrogen source to replace commercially available yeast powder, a threonine fermentation test was carried out, and the average fermentation parameters of 5 consecutive tanks were respectively:

[0027] Using the enzymolyzed protein paste prepared in Example 1 as a nitrogen source, the fermented broth produced 12% acid, the fermentation period was 28 hours, and the fermentation sugar-acid conversion rate was 66%.

[0028] Using commercially available yeast powder as a nitrogen source, the fermentation broth produced 14% acid, the fermentation period was 28 hours, and the fermentation sugar-acid conversion rate was 67%.

[0029] Through the above comparison, the product produced by the present invention can completely replace commercially available yeast powder products for threonine fermentation, thereby greatly reducing the cost of threonine fermentation.

[0030] Through high-performance liquid chromatograp...

Embodiment 3

[0032] The enzymolyzed protein paste prepared in Example 1 is dried by spray granulation fluidized bed, and the obtained finished product is packaged into enzymatic bacterial protein powder (hot air temperature of spray granulation fluidized bed<150°C), replacing the standard The yeast powder in the YPD medium, and the rest of the ingredients remain unchanged. Saccharomyces cerevisiae and Pichia pastoris are cultivated under the same conditions, and the culture effect of the product is evaluated by comparing the growth of the cells.

[0033]

[0034] Determination of OD by nephelometric method 600 The growth of the cells was characterized, indicating that this product can replace commercially available yeast powder products and achieve the desired effect.

[0035] Compared with the conventional process, the present invention has stable and reliable product quality and greatly reduced production cost. For monosodium glutamate production enterprises, it can not only reduce en...

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Abstract

The invention relates to a method for treating threonine mother liquor, and concretely relates to the method for treating other waste liquid by using compound enzyme to perform enzymolysis and fermentation of waste thallus and by using a bipolar membrane electrodialysis treatment. According to the invention, the problem of effective components separation on threonine mother liquor, threonine fermentation residual sugar and other components are fully utilized, the method of the invention has the advantages of high energy efficiency, less waste water emission and low pollution; in addition, an electrodialysis technology has the advantages of simple operation, no pollution, long service life and the like, thereby the investment cost of enterprises can be reduced, the energy saving and emission reduction can be carried out, so that the method of the invention serves multiple purposes.

Description

technical field [0001] The invention belongs to the technical field of threonine production, and relates to a method for treating threonine mother liquor, specifically a method for using compound enzymes to enzymolyze and ferment waste cells and using bipolar membrane electrodialysis to treat other waste liquors. Background technique [0002] Threonine, scientific name 2-amino-3-hydroxybutyric acid. Threonine is an essential amino acid. Threonine is mainly used in medicine, chemical reagents, food fortifiers, feed additives, etc. In particular, the amount of feed additives is increasing rapidly. It is often added to the feed of immature piglets and poultry. It is the second limiting amino acid in pig feed and the third limiting amino acid in poultry feed. Adding L-threonine to the compound feed has the following characteristics: ① It can adjust the amino acid balance of the feed and promote the growth of livestock; ② It can improve the meat quality; ③ It can improve the nut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07C229/22C07C227/40C07H1/08C07H3/04C07H3/06
Inventor 唐永强冯珍泉汲广习马仕敏吕成德
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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