15 results about "Cellular protein" patented technology

The application belongs to the technical field of genetic engineering, and particularly relates to application of NtNRAMP2 protein, a method for improving tobacco cadmium stress resistance and a method for obtaining tobacco plants with cadmium stress resistance. The application provides application of NtNRAMP2 protein in creating tobacco with cadmium stress resistance or breeding tobacco with cadmium stress resistance, and an amino acid sequence of the NtNRAMP2 protein is shown as SEQ ID NO:2. The NtNRAMP2 protein is synthesized by NtNRAMP2 gene, and it is proved by transgenic and functional identification that the NtNRAMP2 natural resistance macrophage protein synthesized by the NtNRAMP2 gene is closely related to tobacco cadmium absorption regulation.

The present invention discloses the use of Avn Anthramides C (Avn C) as an inducer of chondrocytic differentiation in Mesenchymal Stem Cells (MSCs), providing a promising approach for regenerative cartilage therapy. By employing Avn C treatment, MSCs exhibit enhanced chondrocytic differentiation and elevated expression of key specific chondrogenic extracellular matrix (ECM) factors, notably Collagen Type II (COL2A1). Furthermore, when Avn C is used in combination with Chondroitin Sulfate (CS), a synergistic effect is observed, leading to upregulation of the transcriptional activity state of SOX9, a key regulator of chondrogenesis. The disclosed invention shows significant improvements in mRNA gene expression (ACAN, LRP1, SOX9), cellular protein levels (COL2A1, SOX9, LRP1), and functional outcomes, as evidenced by the secretion of extracellular matrix proteins (FSTL-1, BGH3, CO1A1, CO1A2, and CO3A1).

The invention relates to the technical field of artificial dermis, in particular to a preparation method and application of artificial dermis based on bionic acellular matrix. The artificial dermis comprises a bionic acellular matrix component A and an auxiliary material component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, mother cell protein and glucan; the component B comprises antibacterial peptide, antibiotics and a high-molecular polymer material; the mass ratio of the component A to the component B is (4-6): (1-4). The preparation method comprises the following steps: respectively preparing procedure mother liquor of the component A and the component B, preparing hydrogel with a double-layer gradient structure through step-by-step crosslinking, and then carrying out freeze drying and sterilization treatment. The artificial dermis disclosed by the invention has good biocompatibility and antibacterial property, can effectively promote wound healing, is suitable for repairing acute and chronic wounds of four limbs, faces and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

This invention belongs to the field of biomedical technology, specifically relating to a delivery system for type III collagen mRNA and its applications. The nucleic acid delivery system of this invention includes a delivery carrier and a delivered nucleic acid, wherein the nucleic acid is collagen mRNA, and the delivery carrier includes one or more of modified amino acids, growth factors, and polymeric materials. Through intradermal injection, the delivery system of this invention can be administered to the dermal layer of the skin and enter relevant cells, utilizing endogenous cellular proteins to achieve mRNA transfection, forming natural human type III collagen. This invention avoids the immunogenicity risks and structural inactivation problems associated with exogenous collagen injections, and can efficiently and safely replenish non-regenerative type III collagen in tissues. It can be used for various indications such as treating skin aging, promoting wound healing, reducing scar formation, and improving vascular elasticity.

The application discloses a Candida utilis strain for efficiently converting ethanol / acetic acid and application thereof, and belongs to the technical field of single-cell protein production. In view of the technical problems of limited biomass accumulation, low carbon source conversion efficiency and low protein yield of a wild-type Candida utilis strain when the mixed carbon source of ethanol and acetic acid is used, the application provides the Candida utilis TU546 strain with the preservation number of CGMCC NO. 39030, and the strain is cultured in a fermentation medium containing the mixed carbon source of ethanol and acetic acid, so that the fermentation strain body with high protein content is obtained, and efficient conversion from the mixed carbon source of ethanol and acetic acid to single-cell protein is realized. The strain and the method thereof are mainly used for producing single-cell protein products, and can be used as feed or food additives, and have important application value in the microbial fermentation industry.

The present application relates to the technical field of artificial dermis, and particularly relates to a preparation method and application of artificial dermis based on biomimetic acellular matrix. The artificial dermis comprises a biomimetic acellular matrix component A and an auxiliary component B, wherein the component A comprises type I collagen, type VI collagen, elastin, fibronectin, laminin, matricellular protein and dextran; the component B comprises antibacterial peptide, antibiotic and high molecular polymer material; the mass ratio of the component A to the component B is (4-6):(1-4). The preparation method comprises the steps of respectively preparing A and B component process mother liquor, step-by-step crosslinking to prepare a hydrogel with a double-layer gradient structure, and then freeze-drying and sterilization treatment. The artificial dermis has good biocompatibility and antibacterial performance, can effectively promote wound healing, and is suitable for repairing acute and chronic wounds of limbs, facial parts and trunk parts, and is particularly suitable for repairing complex wounds with exposed bones, tendons, nerves and blood vessels.

The invention discloses a method for verifying combination of a small molecule drug and a cell target protein, and belongs to the field of cytobiology and molecular biology. The method comprises the following steps: combining a small molecule drug with a cell target protein, and reducing the content of a free drug; the primary antibody is combined with cell protein to form a primary antibody complex; capturing a primary antibody complex by the PVDF membrane for incubating the secondary antibody; taking out the PVDF membrane, cleaning, and splitting miscellaneous protein in the solution to dissociate residual drugs; and detecting the amount of residual free drugs by HPLC, and analyzing data. The method solves the problem that small molecule-protein binding is difficult to directly detect in a traditional method, and the sensitivity is improved through combination of immunocapture and liquid chromatography or mass spectrometry quantitative detection. According to the method, a trace reagent system is adopted, the dosage of a single sample antibody is reduced by 70%, the PVDF membrane can be repeatedly used for 3-5 times, and the comprehensive detection cost is reduced by 60-80%. Various drug properties can be adapted (by adjusting lysis conditions such as pH / temperature).

The invention provides a composition for inhibiting protein carbonylation and application of the composition. The composition of the present application comprises a first component and a second component, the first component comprising an umbelliferae carrots extract, a cruciferae brassica extract, an azaleaceae vaccinium extract, and an amaranaceae spinach extract as active ingredients; the second component comprises tocopheryl acetate as an active ingredient, and the mass ratio of the active ingredient of the first component to the active ingredient of the second component is 1: 20000 to 3.2: 1. By utilizing the composition provided by the invention, cell protein carbonylation caused by oxidative stress can be delayed, so that the aging state of a human body is improved. The main components of the composition are plant extracts, so that the composition is low in skin irritation, safe, effective and environment-friendly, and a new direction is developed for the development of pure natural whitening and anti-aging cosmetics. The invention further provides a preparation method of the composition and application of the composition in preparation of products for inhibiting protein carbonylation.

The invention discloses a composite single-cell protein feed for improving anti-infection immunity of micropterus salmoides and application of the composite single-cell protein feed, and belongs to the technical field of feed preparation. The feed comprises composite single-cell protein for replacing 40-100% of fish meal, and the composite single-cell protein comprises chlorella and clostridium ethanol protein in a mass ratio of 1: (4-5). The compound single-cell protein is adopted to replace part of fish meal in the feed, so that the cost of the feed can be remarkably reduced, the anti-infection immunity of the micropterus salmoides to aeromonas hydrophila can be improved, expression down-regulation of a pro-apoptosis gene casp8 and an antigen presentation related gene mhci in the micropterus salmoides can be promoted, and the apoptosis resistance of the micropterus salmoides can be improved. The expression up-regulation of the anti-apoptosis gene bcl2l1 and the anti-ferroptosis gene gpx4a is promoted. In addition, the liver metabolism function of the micropterus salmoides can be improved, and lipid metabolism is promoted.

The invention discloses a bacterial contamination control method and system for continuous fermentation of methanol-utilizing bacteria, and belongs to the technical field of microbial fermentation. The method comprises the following steps: in a continuous fermentation process of producing single-cell protein by using methanol, inputting clear liquid to be recycled obtained after thallus separation and a fermentation raw material methanol into a mixing storage tank for mixing and temporary storage, and supplementing the mixture as a fermentation culture medium component into a fermentation tank. The method provided by the invention can solve the problem of easy bacterial contamination in a clear liquid recycling link, is widely applicable to various methanol-utilizing bacteria, realizes long-period stable fermentation, and has significant industrial application value.

This invention provides a SNP site, identification kit, identification method, and application for the Jk(a+w) blood type, which triggers immune hemolytic transfusion reactions. The SNP site is the c.613C>T mutation at position 613, starting from the start codon of the SLC14A1 gene coding region. In the proband, the c.613C>T mutation was detected in one allele, while the other allele carried other known inactivating mutations. The allele carrying the c.613C>T mutation resulted in reduced expression of the Kidd protein in the proband's erythrocytes; the other allele, carrying other inactivating mutations, did not express the Kidd protein. The combined effect of these two mutated alleles resulted in the Jk(a+w) phenotype. This provides a genetic variation basis for the establishment of rare blood type banks and the assurance of transfusion compatibility.

The invention discloses a candida utilis strain capable of efficiently converting ethanol / acetic acid and application of the candida utilis strain, and belongs to the technical field of single-cell protein production. Aiming at the technical problems of limited biomass accumulation, low carbon source conversion efficiency and low protein yield when a wild type candida utilis strain utilizes an ethanol-acetic acid mixed carbon source, the invention provides a candida utilis TU546 strain with the preservation number of CGMCC NO.39030, and the candida utilis TU546 strain is cultured in a mixed carbon source fermentation culture medium containing ethanol and acetic acid. The high-protein-content zymophyte is obtained, so that high-efficiency conversion from the ethanol-acetic acid mixed carbon source to the single-cell protein is realized. The strain and the method thereof are mainly used for producing a single-cell protein product, can be used as a feed or food additive, and have important application value in the microbial fermentation industry.

The application belongs to the technical field of microorganisms, and specifically discloses a Candida utilis, a fermentation method and application thereof. The classification and naming of the strain is Candida utilis, which has been preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC NO:33529. The Candida utilis disclosed in the application can efficiently utilize various low-value industrial and agricultural by-products as carbon sources, and the crude protein content of the dry weight of the fermentation bacteria can be stably above 50%. In addition, the strain has excellent tolerance, can stably grow in the range of pH 3.5-6.0, and can tolerate an ammonium sulfate concentration as high as 15%. The strain of the application solves the problems of low protein yield and narrow substrate utilization spectrum of the yeast strain in the prior art, and provides important technical support for low-cost and high-efficiency production of single-cell protein.

The invention relates to pichia guilliermondii and application thereof in treatment of pea vermicelli wastewater, and belongs to the technical field of microbial technology and environmental engineering. The Pichia guilliermondii KWD1 can tolerate high-concentration COD (Chemical Oxygen Demand), has a relatively good removal effect on COD, ammonia nitrogen, total nitrogen and total phosphorus, can tolerate an acidic pea vermicelli wastewater environment, and can be used for treating wastewater and producing single-cell protein at the same time. According to the single-cell protein produced from the pea vermicelli wastewater, the heavy metal residual quantity meets the requirements of the national feed hygiene standard (GB 13078-2017); the protein product contains seven essential amino acids, has balanced amino acid composition characteristics, has practical and positive application prospects as a substitute protein source in the feed industry, and can be used as a protein supplement source to be matched with other feeds especially in the fields of aquatic products and livestock feeds.

The invention discloses a process for preparing single-cell protein through efficient utilization of methanol and metabolic optimization, and belongs to the technical field of microbial fermentation. Mixing the modified pichia pastoris liquid and the fermentation substrate mixed liquid, and performing fermentation treatment to obtain fermentation liquid; sequentially performing centrifugal separation and vacuum drying on the fermentation liquor to obtain a single-cell protein mixture. According to the invention, pichia pastoris is used as a mutant strain, and after culture activation, the pichia pastoris is sequentially subjected to heavy particle radiation mutagenesis treatment and organic solution mutagenesis treatment, and dual mutagenesis treatment, so that the cell structure can be strengthened, and the capability of producing single-cell protein by using the pichia pastoris through fermentation by using a carbon resource is further improved. Then, selective breeding culture is performed through a special culture medium to obtain modified pichia pastoris liquid, and finally, the modified pichia pastoris liquid is inoculated to special fermentation substrate mixed liquid for fermentation, so that the yield of single-cell protein is increased finally.