20 results about "Cellular proteins" patented technology

The present invention relates to compositions and methods for subpopulation-specific modulation of cell function and for treating repeat expansion disorders comprising genetic, degenerative, neurological and cellular diseases, including immune disorders. Also provided are research kits for subpopulation-specific modulation of protein activity and the corresponding potential to discover novel therapeutic compositions. More specifically, the disclosed compositions and methods selectively up- or downregulate at least a first population of a cellular protein or therapeutic target with minimal or negligible effect on the activity of at least a second population of the cellular protein or therapeutic target.

The present application relates to the field of feed raw material crushing device, specifically to a single-cell protein feed raw material processing crushing device, including the crushing tank, the top of the crushing tank is fixedly installed with the driving motor, the lower part of the inner wall of the crushing tank is fixedly connected with the filter plate, the top of the filter plate is rotatably connected with the transmission rod, and the top of the transmission rod is fixedly connected with the rotating end of the driving motor, further including: fiber collection mechanism. The single-cell protein feed raw material processing crushing device adjusts the cooperation of the adjusting ring and the expansion plate, periodically drives the movable blade disc to approach the fixed blade disc, in this process, on the one hand, the fiber wound on the fiber collection rod is pushed into the cutting area, on the other hand, the sharp blades arranged in a staggered manner are used for accurately and efficiently secondary crushing of the fiber, which makes the waste originally affecting production be converted into fine powder and re-mixed into the feed product, thus eliminating the hidden trouble and improving the comprehensive utilization rate of the raw material.

The invention provides a system and a method for realizing mitochondrial protein localization analysis through base editing and sequencing, which are a simplified platform without radioactive substances and are used for reporting the input and localization conditions of proteins to a mitochondrial matrix. The system and method of the invention can convert a mitochondrial input event into C-to-U base editing of a specific site in a mitochondrial genome. Subsequently, these edits are quantitatively analyzed by sequencing, enabling sensitive and scalable analysis of mitochondrial protein input under various genetic and chemical conditions. Different from a traditional input detection method, the system and the method provided by the invention integrate sensitivity, specificity and high throughput, and provide a multifunctional framework for analyzing subcellular protein localization change under genetic or chemical disturbance.

The invention belongs to the technical field of biological medicine, and particularly relates to application of beta cell protein in preparation of medicine for preventing and treating abortion. Research finds and verifies for the first time that application of beta cell protein (BTC) promotes invasion and tubulation processes of extravillus trophoblast cell line-HTR8 cells, BTC has an obvious rescue effect on embryo absorption in abortion model mice in early and middle stages of pregnancy, and BTC is injected into the abortion model mice in an intraperitoneal cavity in early and middle stages of pregnancy to increase placenta weight, so that the abortion model mice in early and middle stages of pregnancy have an obvious rescue effect on embryo absorption. Therefore, the BTC has a certain treatment effect on the abortion, so that the BTC has a good practical application value.

The present application provides a base model processing method and device for single-cell proteome detection data, which comprises: obtaining single-cell proteome detection data, uniformly processing protein identification information, and standardizing protein abundance data according to detection technology types to obtain uniformly distributed protein expression data; mapping relevant information into identification and abundance embedding vectors respectively and fusing them, adding a special marker vector to construct cell sequence data; inputting the cell sequence data into a base model for pre-training, optimizing parameters through a mask reconstruction and global prediction task to obtain a pre-training model; and fine-tuning the model for a target analysis task, analyzing and processing to-be-processed data to obtain downstream results. The present application can integrate single-cell proteome data of different detection technologies and batches, effectively remove batch effects, extract stable cell low-dimensional representations, support multi-downstream task fine-tuning, realize undetected protein inference and cell trajectory analysis, and improve data utilization efficiency and task adaptability.

The present invention relates to a composition comprising a yeast single cell protein (SCP) product comprising at least about 14% (w / w) of essential amino acids per gram of dried yeast SCP product. Preferably, the yeast single-cell protein product is produced by using ethanol or molasses as a raw material. The yeast single cell protein product advantageously contains high ratios of unsaturated and saturated fatty acids.

The application discloses a capturing device suitable for capturing rare cells and a preparation method thereof. The capturing device suitable for capturing rare cells comprises a substrate with at least a metal surface layer, a coating layer arranged on the surface of the substrate and a capturing agent connected to the coating layer, the capturing agent can specifically combine with target rare cells, and the coating layer comprises mercapto polyethylene glycol carboxyl and a mercapto compound. The application can reduce non-specific adsorption of background cells and proteins, and improve the purity of captured target cells.

A system and method for fixing carbon dioxide by fermentation are provided. More specifically, the disclosure includes the steps of producing methane by fermenting carbon dioxide with methanogenic archaea and producing a single-cell protein supplement. The disclosure further provides integration of methane-producing fermentation with additional methods to achieve improved carbon efficiency.

The application belongs to the technical field of biological medicine, and particularly relates to application of beta cell protein in preparation of medicines for preventing and treating abortion. The application finds and proves for the first time that the application of beta cell protein (BTC) promotes invasion and tube formation of the extravillous trophoblast cell line-HTR8 cells, BTC has obvious rescue effect on embryo resorption in a mouse model of early-mid pregnancy abortion, and BTC increases placental weight and improves placental development when injected into the mouse model of early-mid pregnancy abortion, thereby indicating that BTC can have certain therapeutic effect on abortion, and thus has good practical application value.

The invention discloses a cell preservation method and application, and the cell preservation method comprises the following steps: obtaining a target cell, and fixing the target cell; wrapping the fixed target cells with gel; the gel contains carbomer, a protease inhibitor, a cell permeation agent, a preservative and a buffer component, and directly preserving the target cells wrapped by the gel or embedding the target cells in paraffin to obtain a cell paraffin block for preservation. The cells fixed by the method can be directly preserved for a long time at 4 DEG C without dehydration, transparency and embedding, the gel concentration is adjusted to be liquefied before use, and the fixed gel can be removed by centrifugation; or paraffin embedding can be carried out according to a general process after gel wrapping, and paraffin is sliced before use. Therefore, the cell protein can be stored for a long time without complete denaturation, and meanwhile, an antigen repair process is not needed during use, so that the time is saved, and the efficiency is improved.

The invention discloses kluyveromyces marxianus and application of the kluyveromyces marxianus in production of single-cell protein. The kluyveromyces marxianus disclosed by the invention is a wild strain separated from soil, and has the characteristics of wide growth temperature range and high temperature resistance; monosaccharide and disaccharide obtained by degrading lignocelluloses such as glucose, xylose and cellobiose and tricarboxylic acid cycle metabolites of microbial cells such as succinic acid, citric acid, malic acid and fumaric acid can be rapidly metabolized, and microbial protein can be accumulated. The invention provides an excellent strain capable of being used for producing single-cell protein, and the strain has wide application value in the field of microbial protein.

The utility model is suitable for the technical field of single-cell protein preparation, and provides a fermentation device for single-cell protein preparation, which comprises a mounting plate, the fermentation tank is fixedly mounted at the top of the mounting plate, the fermentation tank is used for fermenting single-cell protein preparation, a heating plate is arranged on the fermentation tank, and the heating plate is used for heating the interior of the fermentation tank; the mounting frame is fixedly mounted at the top of the mounting plate, a centrifugal box is arranged on the mounting frame, and the centrifugal box is used for performing centrifugal operation on the fermented single-cell protein; the plurality of placing cavities are formed in the centrifugal box, and the placing cavities are used for placing the fermented single-cell protein; and the centrifugal mechanism is assembled on the mounting frame and the centrifugal box. The fermentation device for preparing the single-cell protein can ferment the single-cell protein, and can perform centrifugal treatment on the fermented single-cell protein solution, so that the preparation efficiency of the single-cell protein is improved.

The present invention relates to a method for producing a high protein (microbial) biomass or SCP from an alcohol feedstock. In particular, an object is to provide a process for preparing such high protein (microbial) biomass wherein the oxygen delivery capacity is improved. The present invention relates to a method for producing biomass, in particular single-cell proteins, wherein a yeast single-cell protein product comprises yeast cells of the Saccharomyces cerevisiae; and an animal feed comprising such biomass.

The present invention relates to compositions and methods for subpopulation-specific modulation of cell function through spatiochemical and spatiotemporal control over intracellular regulatory protein networks at the level of expressed gene products of nucleic acid-binding protein (NABP) genes. Subpopulation-specific cytoactive agents of the invention are used in therapeutic compositions that up- or downregulate disease-associated intracellular regulatory protein genes at the RNA and protein level for prevention and treatment of plant, animal and human diseases. Applications in human clinical medicine include biopharmaceutical preparations and associated treatment methods for genetic, degenerative, neurological, cellular and immune disorders resulting from dysregulation of endogenous NABP regulatory networks. Therapeutic compositions designed or selected for use in plants and nonhuman animals are applied to the fields of agriculture and veterinary medicine. Also provided are research kits for subpopulation-specific modulation of protein activity and the corresponding potential to discover novel therapeutic compositions across fields. More specifically, the disclosed compositions and methods selectively up- or downregulate at least a first subpopulation of a cellular protein or therapeutic target without substantially altering the activity of at least a second subpopulation of the cellular protein or therapeutic target.

The utility model discloses a hemostasis device, which relates to the technical field of medical equipment and comprises a contact piece, a negative pressure generating device and an electrifying component, the contact piece is provided with an attraction channel penetrating in the axial direction, and the contact piece is used for making contact with a wound. The negative pressure generating device is communicated with the suction channel; the energizing assembly is used for switching on an external power supply to conduct electricity to the contact piece. According to the technical scheme, the contact piece is in contact with the wound of the human body, blood in the wound is sucked out through the negative pressure generating device, then high-frequency current is led into the contact piece through the electrifying assembly, tissue making contact with the contact piece is locally heated, when the temperature rises to a certain degree, cell protein of the tissue is denatured and solidified, and meanwhile blood vessels are shrunk; therefore, the technical effect of rapid hemostasis is achieved.

The invention discloses a method for researching the effect of dermatopontin in diabetic nephropathy, and belongs to the technical field of medicine application research. Selecting mice, dividing the selected mice into an experimental group and a control group, feeding the mice, performing blood glucose and diabetic nephropathy modeling, treating the mice, separating kidneys, and performing cold storage; the refrigerated kidney cells are subjected to resuscitation, cell passage and cell freezing treatment; dividing the frozen cells into a control group, a high glucose group and a hypertonic group, performing culture medium treatment with different concentrations, and then collecting cell protein and RNA for CCK-8 experimental treatment; carrying out DPT recombinant protein intervention, cell lentivirus infection and gene knock-down stable strain screening; the method comprises the following steps: extracting cell total protein, measuring protein concentration, carrying out Western Blot western blot experiment, extracting cell total RNA, carrying out reverse transcription and fluorescent quantitative PCR, carrying out immunohistochemical staining, and carrying out statistical analysis on a result according to data. The result of the invention corresponds to the report that DPT is used as a fibrosis promoting factor in fibrosis of other organs, and the conservative function of DPT in the fibrosis process is proved.

The invention discloses a method for fermentation treatment of composite wastewater and joint production of single-cell protein. The method comprises the following steps: (1) carrying out multiplication culture on a microbial strain by using a basic culture medium until the OD600 of fermentation liquor reaches 50-100; and 2) feeding composite wastewater into the fermentation liquid to continue fermentation, stopping fermentation when the volume of the fermentation liquid reaches 2-3 times of the initial volume, and performing solid-liquid separation to obtain single-cell protein. According to the invention, the composite wastewater obtained by compounding two specific process wastewater in a fixed proportion is used as a fermentation raw material, so that the synergistic metabolism of organic matters can be realized to efficiently produce the single-cell protein, the utilization rate of each organic matter component in the wastewater is up to 90% or more, and meanwhile, the biochemical index BOD / COD of the residual fermentation liquor is increased to 0.5 or more.

A continuous methanol single-cell protein production method and device, the production method is applied to the production device, including the following steps: the three-dimensional physical reaction space in the fermentation device is discretized into a three-dimensional body grid structure, and a plurality of flow field discrete sampling nodes are defined at the grid intersection; the real-time fluid viscosity is calculated, the partial pressure sampling dissolved oxygen value is weighted and compensated in the spatial dimension, and a global dissolved oxygen characterization tensor covering the whole space is reconstructed; the metabolic activity of microorganisms corresponding to each flow field discrete sampling node is evaluated, and a virtual metabolic field map is generated; the metabolic low-activity area in the virtual metabolic field map is identified, and a spatial differential compensation is driven to be performed by an execution unit. The present application realizes accurate reconstruction and prediction of the metabolic state of the whole space, greatly suppresses the environmental gradient through spatial differential compensation, significantly reduces the operation cost and load of the precision monitoring system, and improves the safety and product consistency of the bacterial growth.

The present invention relates to a meat analogue product comprising a textured vegetable protein, a binder, a flavouring agent and 0.5 to 10% (w / w) of a yeast single cell protein product wherein the yeast single cell protein product comprises Saccharomyces yeast cells or parts thereof.

The invention relates to the field of feed raw material crushing devices, in particular to a crushing device for processing single-cell protein feed raw materials, which comprises a crushing tank, a driving motor is fixedly mounted at the top of the crushing tank, a filter plate is fixedly connected to the lower part of the inner wall of the crushing tank, and a transmission rod is rotatably connected to the top of the filter plate. And the top of the transmission rod is fixedly connected with the rotating end of the driving motor. According to the smashing device for single-cell protein feed raw material processing, through cooperation of an adjusting ring and a telescopic plate, a movable blade disc is driven to periodically approach a fixed blade disc, and in the process, on one hand, fibers wound on a fiber collecting rod are pushed into a cutting area; and on the other hand, the fibers are subjected to precise and efficient secondary crushing through the sharp blades which are arranged in a staggered mode, so that waste materials which originally influence production are converted into fine powder, the fine powder is re-mixed into feed products, the hidden trouble of faults is eliminated, and the comprehensive utilization rate of raw materials is improved.