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Method for preparing hydrolyzed oligosaccharides and short peptides from salt algae residues

A technology of oligosaccharides and salina peptides, which is applied in the field of salina utilization, can solve the problems of salina polysaccharides and protein utilization, and low resource utilization, so as to improve human immune function, high raw material utilization, and facilitate popularization and application Effect

Inactive Publication Date: 2009-06-03
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method only extracts small molecular nutrients in salina residue, such as minerals, amino acids, etc., but does not utilize salina polysaccharides and proteins, and the utilization rate of resources is extremely low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The specific steps of a method for preparing hydrolyzed oligosaccharides and short peptides from salina are as follows:

[0040] (1) Separation of salina residue

[0041] With the salina slag powder that extracts β-carotene as raw material, by the weight (gram) of salina slag powder: the volume (liter) ratio of deionized water is the ratio of 1:5, first adds deionized water in the container, Then add the salt algae residue powder, stir until the salt algae residue powder is evenly dispersed, then use ultrasonic treatment for 30 minutes, and then centrifuge at a speed of 10,000 rpm for 5 minutes to collect the lower sediment and supernatant respectively. The collected lower precipitate is used to prepare hydrolyzed salina peptide, and the collected supernatant is separated by ultrafiltration with an ultrafilter with a molecular weight cut-off of 5KDa, and then the ultrafiltrate and the intercepted concentrate are collected separately. The filtrate contains small Molecul...

Embodiment 2

[0065] The specific steps of a method for preparing hydrolyzed oligosaccharides and short peptides from salina are as follows:

[0066] (1) Separation of salina residue

[0067] With embodiment 1. It is characterized in that: the ratio of the weight (g) of salina slag powder: the volume (liter) of deionized water is 1:8, the ultrasonic treatment is 45 minutes, the centrifugation speed is 12000 rpm, and the centrifugation time is 10 minutes.

[0068] (2) Preparation of skin energy activation liquid

[0069] With embodiment 1. It is characterized in that: use potassium hydroxide (KOH) solution to adjust the pH value to 5.8, and carry out vacuum concentration to a soluble solid concentration of 11% at a temperature of 85°C and a vacuum degree of 0.07Mpa to prepare a K ion concentration The skin energy activation liquid is 50mg / mL, amino acid concentration 22mg / mL and phosphate concentration 15mg / mL, and its yield is 10% of the mass percent of de-β-carotene salt algae residue p...

Embodiment 3

[0091] The specific steps of a method for preparing hydrolyzed oligosaccharides and short peptides from salina are as follows:

[0092] (1) Separation of salina residue

[0093] With embodiment 1. It is characterized in that: the ratio of the weight (grams) of salina slag powder: the volume (liter) of deionized water is 1:10, the ultrasonic treatment is 60 minutes, the centrifugation speed is 13000 rpm, and the centrifugation time is 20 minutes.

[0094] (2) Preparation of skin energy activation liquid

[0095] With embodiment 1. It is characterized in that: use potassium hydroxide (KOH) solution to adjust the pH value to 6.0, and carry out vacuum concentration to a soluble solid concentration of 12% under the conditions of a temperature of 90°C and a vacuum degree of 0.08Mpa to prepare a K ion concentration The skin energy activation liquid is 60mg / mL, amino acid concentration is 25mg / mL and phosphate concentration is 16mg / mL, and its yield is 12% of the mass percent of de...

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Abstract

The invention provides a method for preparing hydrolyzed oligosaccharides and short peptides from salt algae residues, which belongs to the technical field of salt algae utilization. The method adopts beta-carotene removed salt algae residues as raw materials, separation and enzymatic hydrolysis are repeatedly performed, and then various products are made after concentration and drying. The method provided by the invention comprehensively utilizes the salt algae residues to make various daily use chemicals, medicines and health products, such as skin energy activating solution, hydrolyzed salt algae oligosaccharides and polysaccharide and short peptides. The residue and precipitate of the hydrolyzed salt algae is retained, but not discarded during production process, and then is used as fermentation base material for microbial fertilizer preparation. The products prepared through the method can be wildly applied to products for skin care, moisture preservation, nutrition, antioxidation and anti-ageing, can be applied to health products and anti-tumor products, and also can be served as single cell protein products to complement nitrogen nutrition and radioprotective products.

Description

1. Technical field [0001] The invention belongs to the technical field of salina utilization, and in particular relates to a method for preparing salhalose and short peptides from salina residues from which beta-carotene has been removed. 2. Background technology [0002] Salina, also known as salina, is a green single-celled algae with a tiny shape and a coating formed by glycoproteins. It is mainly distributed in coastal waters, and its adaptability to salinity ranges from 0.2 to 3.5%. It has the characteristics of fast reproduction, strong viability, and suitable for breeding. my country has a long coastline and a large number of inland salt lakes suitable for the survival of salina, so salina is widely distributed and rich in resources. Salina is rich in oil, β-carotene, protein, polysaccharide compounds, Ca, P, Zn and other trace minerals, including 8 kinds of amino acids necessary for human beings and other series of available substances. At present, Inner Mongolia J...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/04C12P19/00C12P21/06C08B37/00C07K1/34
Inventor 魏顺安周小华金挺廖妙飞苏霞利陈林赵成霞
Owner CHONGQING UNIV
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