90 results about "High throughput analysis" patented technology

The invention provides an exosome automatic identification and analysis method based on microscopic imaging, and belongs to the technical field of exosome identification and analysis, and the method comprises the steps: collecting an exosome fluorescence microscopic image, carrying out the preprocessing, and obtaining an exosome region through adaptive threshold segmentation and morphological processing. A GPU is adopted to process and extract morphological, texture and statistical feature matrixes in parallel, and meanwhile, the CPU recognizes an abnormal region. And combining the features to form a region feature set, inputting the region feature set into the preliminary screening model for scoring and screening high-credibility regions. Deep feature extraction is carried out on the high-credibility region, and multiple features are fused by using an attention fusion mechanism to construct vectors; and inputting a depth classifier to obtain a classification probability matrix and a screening result. And finally outputting the number, position, classification and credibility score of the exosomes. The problems that an existing exosome analysis method often depends on manual recognition, and automatic and high-throughput analysis is difficult to achieve are solved.

The present invention discloses a combination of different nucleic acid aptamers with 291 known targets. By performing sequencing analysis on the nucleic acid aptamer sequences of this combination through single-cell high-throughput sequencing technology, a cell atlas targeting mononuclear cells derived from the peripheral blood of patients with cholangiocarcinoma can be constructed. The nucleic acid aptamers covered by this combination have high specific affinity for their molecular targets. After extending adapters commercialized for sequencing platforms at both ends of the nucleic acid aptamer sequences respectively, by performing high-throughput sequencing on the nucleic acid aptamer sequences with sequencing adapters bound to the surface of mononuclear cells, high-throughput analysis of biomarkers on the surface of mononuclear cells derived from the peripheral blood of patients with cholangiocarcinoma can be achieved, thereby constructing a cell atlas of mononuclear cells derived from the peripheral blood of cholangiocarcinoma.

The present disclosure relates to methods for high-throughput analysis of proteins and peptides employing a set of engineered binders that recognize terminal amino acid residues of peptide analytes. In particular, disclosed herein is the analysis of occupancy of N-linked glycosylation sites in proteins and peptides. The disclosure finds utility at large-scale profiling of N-linked glycosylation sites, as well as monitoring changes in glycosylation patterns associated with numerous disease conditions.

The invention provides a method for quickly analyzing TEM (Transmission Electron Microscope) images in batches and measuring critical dimensions by nanoscope software, which relates to the technical field of chip manufacturing and comprises the following steps of: constructing a TEM image intelligent analysis and measurement system; the TEM image intelligent analysis and measurement system comprises an image acquisition module, an image preprocessing module, a measurement template generation module, a measurement path planning module, a data fusion and analysis module and a cloud optimization module, and TEM-Nanoscope in the TEM image intelligent analysis and measurement system comprises a quantum calculation acceleration module (20 quantum bits and 10 CNOT gates) and an A * algorithm path planning module. The feature extraction time of the million-level TEM image is shortened from the hour level to the minute level (the efficiency is improved by 50 times), the path planning time is shortened to 1 second, and the bottleneck of slow processing speed of traditional manual analysis and mainstream software is solved. Compared with one-by-one treatment of DigialMicrograph and ImageJ, the method disclosed by the invention has the advantages that high-throughput analysis is supported, and the production efficiency is greatly improved.

The present invention, among other things, provides technologies for detecting and / or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and / or DNA loci.

The present invention, among other things, provides technologies for detecting and / or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and / or DNA loci.

The present disclosure provides methods, compositions, and kits for the rapid release, labeling, and detection of O-glycans present on a glycoconjugate, such as a glycoprotein, glycopeptide, peptidoglycan, or proteoglycan of interest. The compositions and methods allow the released O-glycans to be subjected to high-throughput analysis.

Provided herein are compositions and methods for the multiplexed profiling of RNA and DNA modifications across transcriptomes and genomes, respectively. The methods combine molecular recognition of non-canonical features (e.g., base modifications, backbone modifications, lesions, and / or structural elements) of a target nucleic acid with a step of writing the information from this recognition event into the neighboring genetic sequence of the target nucleic acid using a barcode. The resultant barcoded nucleic acids are then converted into sequencing libraries and read by DNA / RNA sequencing methods. This step reveals the sequence of the barcode, which is correlated with the non-canonical feature in the target nucleic acid(s). The high throughput profiling methods described herein allow for localization of one or more modifications in a target nucleic acid. The methods also allow for identification of the nature and location of several or all DNA / RNA modifications in parallel.

The present disclosure relates to analysis of single molecule events (40) as observed by irradiating a sample (18) with excitation radiation (14) and detecting emitted radiation (22) from the sample induced by the excitation radiation, wherein the excitation radiation path from the radiation source (12) to the plurality of samples does not overlap the emitted radiation path from the plurality of samples to the detection device (26).

Disclosed are methods and systems for confirming the identification of samples processed by a high-throughput analytical technique. The method may include distributing a plurality of individual samples to individual predetermined positions of a multi-sample test unit and distributing an identifier material to at least one predetermined position of the multi-sample test unit. The method may also include determining the absence or presence of an analyte in the plurality of individual samples, and determining the presence of the identifier material, wherein the presence of the identifier material at the predetermined position of the multi-sample test unit is used to confirm the identity of the plurality of individual samples positioned in the multi-sample test unit. Also disclosed are systems for performing the methods. The methods and systems may be applied to high-throughput LC-MS / MS or other analytical methods.

This invention discloses a droplet microarray chip based on molecular logic operations and its construction method, belonging to the field of biochemical analysis and detection technology. It includes a substrate layer, a functional layer on the substrate layer, and molecular logic circuitry. The functional layer is a superhydrophilic-superhydrophobic patterned porous polymer film prepared in situ on the substrate using digital light processing technology, followed by PFOC acylation. The superhydrophilic-superhydrophobic patterned porous polymer film contains arrayed superhydrophilic microreaction units, separated by superhydrophobic polymer regions. Each superhydrophilic microreaction unit encapsulates an independent microdroplet, and the molecular logic circuitry is encapsulated within each independent microdroplet. This invention, through the combination of droplet microarray chip and molecular logic circuitry, achieves systematic improvements in multi-enzyme synergistic detection capabilities, logic computation complexity, spatial isolation control, high-throughput analysis, and adaptation to biological environments.

The invention belongs to the technical field of luminescence imaging detection, and particularly relates to an electrochemical luminescence imaging detection platform based on a closed bipolar electrode array and application. Comprising a sensor and a detection unit, a bipolar electrode array unit comprises an insulating substrate, an anode region and a cathode region, the anode region and the cathode region are isolated from each other and are of the same structure, and each anode region or each cathode region is provided with a plurality of independent holes which are arrayed in parallel; gold nanoparticles are loaded in a plurality of independent open pores of the parallel array in the anode region, and a Ru (bpy) 32 < + >-TPA system is constructed; au / g-C3N4 composite materials are loaded in a plurality of independent open pores of the parallel array in the cathode region, and an Au / g-C3N4-K2S2O8 system is constructed. According to the invention, based on the bipolar electrode array unit, the terpyridyl ruthenium and the gold / graphite phase carbon nitride are used as different illuminants to realize capture and quantitative analysis for synchronous detection of acetaminophen and rutin, and the detection accuracy and high-throughput analysis are ensured.

The application discloses a kind of based on multi-focal sharpness analysis's stacked cell hierarchy determination method and system.Acquire Z-stack multi-focal image sequence and extract each focal plane cell contour;Based on centroid coordinates and area features, establish the profile correlation data table of the same cell in different focal planes;Ellipse fitting algorithm is used to complete the shape of the profile of the cell that is blocked;Based on complete geometry mask, screen potential stacked candidate cell pair and locate overlapping area profile;With gradient function as evaluation index, calculate the overlapping area profile sharpness of each cell under multiple focal planes, determine the best focus focal length and judge cell hierarchy relationship;Attribute graph is constructed and topological sorting is executed, and global stacked hierarchy sequence is output.The application establishes hierarchy reasoning rule with rigorous physical basis according to the physical law that upper cell best focus focal length is higher, is compatible with existing Z-stack imaging process, adapts to basic laboratory high-throughput analysis demand.

The present invention, among other things, provides technologies for detecting and / or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and / or DNA loci.

The present invention belongs to the field of intelligent biotechnology and relates to a method, system and medium for wheat spike number recognition based on Faster R-CNN, including: extracting the canopy images of wheat spikes of different families, calibrating the wheat spikes in the images to obtain the labels corresponding to each wheat spike; inputting the images and labels of the wheat spikes into a ResNet network model for feature extraction to obtain the feature map of the wheat spikes, and establishing corresponding candidate boxes according to the wheat spike contours; training the Faster R-CNN model through the feature map to obtain an optimal wheat spike recognition model; inputting the wheat spike image to be measured into the wheat spike recognition model to obtain the candidate boxes corresponding to each wheat spike; counting the number of candidate boxes, so as to obtain the number of wheat spikes. It can accurately perform QTL mapping on wheat, can accurately identify wheat with high overlapping characteristics of wheat spike number, and is expected to provide a high-throughput analysis tool for the identification of yield-related phenotypes in wheat molecular breeding.

The invention relates to the technical field of preparation of functionalized inorganic nano materials, in particular to a preparation method of a nano porous rare earth oxide material, which comprises four synergistic steps of precursor preparation, molecular directional modification, secondary construction of a pore structure and high-temperature roasting conversion. The material prepared by the process has a mesopore-macropore composite hierarchical pore structure, is high in specific surface area and large in saturated adsorption capacity on forbidden substance molecules, and can specifically recognize at least three forbidden substances such as morphine, methylamphetamine and ketamine; when the material is applied to a prohibited article detection probe, non-invasive sampling can be realized through surface scratching, and high-throughput analysis is carried out by matching with an in-situ mass spectrometry detection device. The process parameters are controllable, large-scale production is easy, and the application prospect in the field of trace contraband detection is wide.

The invention discloses a method for detecting illegally added compounds in food, which is characterized in that complex matrix interference such as saccharides, fatty acid and phospholipid is efficiently removed through a purification strategy of combining a solid-phase extraction column and an adsorbent, the anti-interference capability of the method is remarkably improved, and the method is ensured to have good accuracy and applicability in various food matrixes. In a separation and detection link, high-throughput analysis for synchronously detecting various illegally added compounds in a relatively short time is realized by optimizing chromatography and mass spectrometry conditions, and the screening efficiency is greatly improved. Based on a multi-reaction monitoring and positive and negative ion switching scanning technology, the method is excellent in sensitivity and selectivity, can cover hormone compounds, PDE5 inhibitor compounds, SSRI compounds and other compounds with remarkable structural difference, and greatly expands the screening range. And the method also has effective identification capability on nafil derivatives, structural analogues and other medicines which are not listed in supervision, and provides reliable technical support for systematic screening and supervision of kidney-tonifying and yang-strengthening illegal additives in food.

The invention discloses a system for detecting ArsM in a sample, a detection method, a detection device and application thereof. The detection system of the present invention comprises a clustered regularly spaced short palindromic repeat (CRISPR)-Cas complex comprising a guide RNA targeting ArsM and a CRISPR-related (Cas) protein or a functional fragment thereof; and a horse radish peroxidase (HRP) complex to which a single-stranded DNA (ssDNA) is linked. According to the invention, a cascade amplification detection system based on CRISPR / Cas12a is constructed, and the cascade amplification detection system is further integrated on a multi-channel paper-based microfluidic device to realize on-site high-throughput analysis of nucleic acid molecules.

The present invention discloses a high-throughput ICP torch, comprising a sample pipeline and a sheath gas pipeline coaxially arranged with the sample pipeline; the sheath gas pipeline comprises an air inlet section, an intermediate section and a contraction section of a tapered structure connected in sequence, the sample pipeline outlet is arranged on the inner side of the air inlet section or the intermediate section, the sample pipeline is used to guide the sample gas into the sheath gas pipeline, the inlet of the contraction section is connected to the intermediate section, the outlet of the contraction section is the sheath gas pipeline outlet, the inlet diameter of the contraction section is larger than the outlet diameter of the contraction section; the contraction section is used to concentrate energy in the torch; the present invention improves the convective heat transfer efficiency, and shows significant advantages in terms of ionization speed, energy consumption, cooling efficiency and analytical throughput. Even at a lower gas flow rate, it can achieve the same cooling effect as a traditional Fasse l torch, ensuring the stable operation of the equipment in high-throughput analysis, extending the service life of the equipment, and reducing maintenance frequency and cost.

The invention discloses a preparation method of a silicon quantum dot-Fe < 3 + > fluorescent probe and application of the silicon quantum dot-Fe < 3 + > fluorescent probe in detection of glyphosate pesticide residues, belongs to the field of analytical chemistry, and solves the problems that an existing detection method is long in detection time consumption, complex in sample pretreatment and difficult in high-throughput analysis. The preparation method comprises the following steps: introducing a newly prepared sodium ascorbate solution and a 3-aminopropyltrimethoxysilane solution into ultrapure water for reaction; dialyzing the solution in distilled water to remove unreacted solvent, and storing the obtained solution in a dark place at room temperature; carrying out rotary evaporation on the solution until the solution is viscous, pumping by a vacuum pump until the solution is dry, and dissolving by distilled water to obtain a silicon quantum dot solution; and incubating the silicon quantum dot solution and Fe < 3 + > at room temperature to form the silicon quantum dot-Fe < 3 + > fluorescent probe. The preparation method is simple, and reaction products are easy to separate and purify; the method is simple and convenient in glyphosate detection operation, good in selectivity, rapid in response and high in sensitivity, and can be used for rapid and quantitative detection of glyphosate pesticide residues in vegetables.

The invention discloses a method for analyzing the interaction between a biomolecular condensate and a drug, and provides a signal report strategy based on the ultraviolet visible light absorption characteristic of the drug, which utilizes the unique space confinement effect of the biomolecular condensate and the influence of a chemical microenvironment obviously different from a water phase on the physicochemical properties of drug molecules to analyze the interaction between the biomolecular condensate and the drug. The color and ultraviolet-visible absorption spectrum change before and after the action of the drug and the biomolecular aggregate is monitored, and the peak shift and intensity change of the characteristic absorption peak are quantitatively analyzed, so that the interaction analysis of the biomolecular aggregate and the drug is finally realized. The analysis method adopted by the invention is simple to operate, does not need fluorescent labeling or centrifugal separation and other processes, can control the interaction analysis time of a single drug and a biological aggregate within 5 minutes, supports high-throughput analysis, and is suitable for large-scale popularization and application. And the interaction between 96 or 384 or more (depending on the specification of the microplate) drugs and molecular aggregates can be analyzed within 5 minutes.

The invention relates to a registration method and system for mouse brain tissue slice images. On the basis of a two-dimensional mouse brain tissue slice image to be registered and a section parameter prediction model, spatial positioning parameters are determined, and tedious three-dimensional space search and iterative matching in the prior art are effectively replaced by one-time prediction based on the section parameter prediction model, so that the matching efficiency is improved, and the robust spatial positioning parameters are given; determining deformation mapping information based on the spatial positioning parameters, the two-dimensional mouse brain tissue slice image and the three-dimensional average mouse brain template map, determining a two-dimensional labeled slice based on the spatial positioning parameters and the three-dimensional standard map, and determining a registered mouse brain tissue slice image based on the two-dimensional labeled slice and the deformation mapping information, according to the method and the system, subsequent fine alignment processing is carried out on a two-dimensional level, so that the calculation complexity and the algorithm difficulty are greatly reduced, an automatic workflow of intelligent three-dimensional positioning and efficient two-dimensional registration can be formed, and powerful support is provided for high-throughput analysis and clinical application.

According to the technical scheme, the real-time continuous blood plasma concentration detection instrument equipment is characterized by comprising a base, a high performance liquid chromatograph is fixedly installed on the top face of the base, a chromatographic column is arranged on one side of the high performance liquid chromatograph, a sliding rod is fixedly installed on the top face of the base, and the sliding rod is arranged on the other side of the high performance liquid chromatograph. A mounting frame is arranged on the top surface of the base, and a sliding hole is formed in the top surface of the mounting frame; the sample adding assembly is arranged on one side of the mounting frame and is used for continuously adding detection samples into the chromatographic column, and by arranging the sample adding assembly, the detection samples can be effectively and continuously added into the chromatographic column, so that the manual operation time and the waiting time for adding the samples every time are shortened, the continuity is higher, and the detection efficiency is improved. The method effectively reduces the white storage period, improves the overall efficiency of the experiment, enables high-throughput analysis to be possible, and is more convenient to efficiently and continuously monitor the blood concentration in real time.

The present disclosure relates to methods for high-throughput analysis of proteins and peptides employing a set of engineered binders that recognize terminal amino acid residues of peptide analytes. In particular, disclosed herein is the analysis of occupancy of N-linked glycosylation sites in proteins and peptides. The disclosure finds utility at large-scale profiling of N-linked glycosylation sites, as well as monitoring changes in glycosylation patterns associated with numerous disease conditions.

The present invention discloses a high-throughput analysis method for phthalate plasticizers based on thin-film microextraction technology, belonging to the technical field of physical and chemical inspection of phthalate plasticizers. The high-throughput analysis method is as follows: after surfactants, urea, tetraethyl orthosilicate, isopropanol, and cyclohexane are added to water and react, they are calcined to obtain nano-mesoporous silica; octadecyltrichlorosilane is used as a modifier to react with the activated mesoporous silica to obtain C18-modified mesoporous silica; then trimethylchlorosilane is used as a capping reagent for capping to obtain C18-modified nano-mesoporous silica; the C18-modified nano-mesoporous silica and polyacrylonitrile are respectively dispersed in DMF, and after mixing, they are sprayed and cured to obtain a microextraction thin film; it is installed in a high-throughput oscillating extraction device, and after the sample undergoes the thin-film microextraction analysis process, reverse-phase liquid chromatography is used for analysis to calculate the content. This method can meet the high-throughput, efficient, and rapid analysis of phthalate esters in a large number of samples.

The present disclosure relates to a screening system configured to identify biological agents, biological variances, pathogens, and / or genetic variances. The screening system may include an incubation zone having an incubation station for incubating multiple samples and a detector for detecting electromagnetic radiation emitted simultaneously or quasi-simultaneously from the multiple samples. The screening system may also include an optional, replaceable incubator unit housed within the incubation station. The incubation station may also include an optically based datum system used as a reference point for orienting an image of the incubation station captured by the detector and, optionally, for assisting in synchronizing the capture of spectral images corresponding to the output of a test for a pathogen or genetic variance.

Methods of generating organoids on multi-well plates are provided by depositing a polymeric solution comprising cells under conditions which result in a homogenous population of organoids, which can be used for high throughput analysis for drug screening and for determining treatment regimens of a drug.

Described herein are fluorescence imaging system designs, flow cell devices and methods thereof that enable imaging of three or more axially displaced surfaces without the use of any optical compensator. The optical systems and flow cell devices herein provide higher throughput analysis for genomics and other imaging applications at lower cost.

A preparation method of an extracellular vesicle drug delivery system belongs to the field of extracellular vesicles, and comprises the following steps: firstly, representing extracellular vesicles through a transmission electron microscope and a dynamic light scattering method, constructing a multi-dimensional feature matrix and calculating a stability coefficient; mixing the screened qualified vesicles with polyethylene glycol, and regulating and controlling the mixing process by adopting an online monitoring system; then adding chitosan and adjusting the pH value, and controlling process parameters through an intelligent automatic titration system; a targeting ligand is added for modification, and a high-throughput analysis system is adopted to evaluate the binding effect; after precise filtration sterilization and ultrafiltration centrifugal separation, a trehalose protective agent is added, finally, drying treatment is conducted through an intelligent freeze drying system, and a final product is obtained. The technical problem of unstable preparation quality caused by insufficient stability control in the preparation process of an extracellular vesicle drug delivery system in the prior art is solved.

This invention discloses a method for constructing and sequencing single-cell multi-omics libraries based on a dual-permeable microarray design, comprising the following steps: S1, performing an in-situ nucleic acid molecular labeling reaction on a single-cell or mononuclear suspension of the tissue to be tested in a multi-well plate to tag it with a known base sequence; S2, mixing the reacted single-cell or mononuclear suspension with microbeads carrying the known base sequence and loading it onto a dual-permeable microarray; after the reaction is complete, sealing the chip with sealing oil; S3, performing nucleic acid molecule polymerization and amplification within the chip, and then collecting the liquid within the chip to construct a single-cell sequencing library; S4, sequencing the single-cell library and processing the data to obtain single-cell multi-omics library data. This invention enables high-throughput analysis of multi-omics genetic information, including transcriptomics and chromatin availability, at the single-cell level at a low cost and with rapid convenience.