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Method for screening malignant ovarian tumor markers from blood serum metabolic profiling

A metabolic profile and ovarian tumor technology, applied in the field of screening of gynecological malignant ovarian tumor serum small molecule metabolic marker profiles, can solve the problems of easy introduction of errors in serum and difficulty in modeling, achieving simple process, saving analysis time, and resolution. high effect

Inactive Publication Date: 2010-07-07
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the complexity of biological samples, errors are easily introduced during the collection, storage and pretreatment of serum; the electrospray ion source itself has a certain attenuation effect, and if the analysis sequence is too long, the mass spectrometry response of the sample will have a certain deviation, which will give Subsequent modeling poses difficulties, which is what metabolomics is currently facing

Method used

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  • Method for screening malignant ovarian tumor markers from blood serum metabolic profiling
  • Method for screening malignant ovarian tumor markers from blood serum metabolic profiling
  • Method for screening malignant ovarian tumor markers from blood serum metabolic profiling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Serum collection

[0028] Before the collection, the included researchers signed the informed consent.

[0029] Inclusion criteria for malignant ovarian tumors: diagnosed as malignant ovarian epithelial tumors, the specific pathological type and stage are not limited. And did not receive any treatment related to the disease before collecting the specimen.

[0030] Inclusion criteria for the healthy control group: normal physical examination population (female between 50-70 years old). Physical examination of healthy people.

[0031]The collection time was from 6:00 to 8:00 in the morning, and the fasting venous blood was drawn. The body temperature of the subjects was between 36°C and 37.5°C, the vital signs were stable, and they were not in the acute stage of various diseases. The collected blood was placed in a negative pressure tube, and the supernatant was obtained after centrifugation for 15 minutes. Serum was stored in an ultra-low temperature freezer (-80°...

Embodiment 2

[0053] In order to illustrate the repeatability of the screening method described in this patent, the applicant performed the same sample analysis and data processing process as model 1 on another 14 cases of malignant ovarian tumor samples and 14 cases of healthy control samples, and established another PLSDA model. is called model 2.

[0054] The difference from Example 1 is that the relevant data of Model 2 are: A=4, R 2 X=0.677, R 2 Y=0.939, Q 2 Y=0.756. Model 2 can also distinguish between malignant ovarian tumors and healthy control samples. Through model 2, among the 10 variables screened out according to the variable importance factors, 8 variables are consistent with Table 1. Using the method described in this patent can screen out basically consistent markers for different groups of people, indicating that the method is indeed stable, repeatable, and not easily affected by the tested population, and is suitable for clinical application.

[0055] Using the 10 mar...

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Abstract

The invention discloses a method for screening malignant ovarian tumor markers according to blood serum metabolic profiling. In the method, the Ultra Performance Liquid Chromatography-mass spectrometry technology is employed to analyze blood serum to obtain blood serum metabolic profiling; then the multivariate statistics method is employed to analyze data concerning malignant ovarian tumor and blood serum metabolic profiling of healthy people, so as to screen maker spectrums. The screening method of the invention features good repeatability and good forecasting property of the screened markers. Proven by discriminant classification analysis, the screening method enjoys an average forecasting precision rate of 90.90% and a positive relevance ratio of 82.65%.The maker spectrum covering multiple compounds can be obtained in a single analysis, which indicates that the marker is suitable for high flux analysis and enjoys the prospect of being promoted to large-scale sample screening and clinical application.

Description

technical field [0001] The invention relates to the fields of analytical chemistry and medicine, and is a new method for screening the serum small molecule metabolic marker profile of gynecological malignant ovarian tumors based on the serum metabolic profile of metabonomics technology. Background technique [0002] Ovarian cancer, endometrial cancer, and cervical cancer are collectively known as the "three gynecological cancers". The incidence of the first two continues to rise, and the proportion of glandular epithelial types of cervical cancer is also increasing, which is seriously endangering women's health. General physical examination and imaging examinations have limited effects, and by the time they can be discovered and diagnosed, it is often in the middle or late stage, lacking early warning value. In the study of ovarian malignant tumors, commonly used serum markers are: CA125 (cancer antigen 125), squamous cell carcinoma antigen, CEA (carcinoembryonic antigen). ...

Claims

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Application Information

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IPC IPC(8): G01N30/78G01N33/48
Inventor 许国旺陈静徐丛剑路鑫张晓燕曹锐万小平吴小华赵素敏
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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