11 results about "High Throughput Assay" patented technology

The invention relates to the technical field of phenotype measurement in aquatic genetic breeding, in particular to a macrobrachium rosenbergii small-size shrimp high-throughput phenotype measurement method and measurement system. According to the method, a camera obscura environment is constructed through a white shooting bottom plate, a dark extinction inner wall and an LED area array light source in a measuring box body, and illumination is adaptively adjusted by combining rough segmentation of a preview and analysis of a local gray histogram, so that stable bimodal gray distribution is formed between a shrimp body and a background. On the basis, a segmentation-key point combined deep learning model is adopted, a shrimp body mask and a standard key point are output at the same time, topological consistency loss constraints of a framework side length proportion and an included angle are introduced in training, and key point mismarking and drifting caused by the transparent body color are remarkably reduced. According to the method, rapid, non-contact, high-precision and high-throughput measurement of macrobrachium rosenbergii small-specification individual phenotype parameters and weight is realized, and a high-quality structured phenotype database is provided for genetic evaluation and multi-character combined selection.

This invention belongs to the field of biotechnology and relates to a specific monoclonal antibody against porcine circovirus type 2 (PCV2) Cap protein and its applications. The CDR of the heavy chain variable region of the monoclonal antibody includes CDR1, CDR2, and CDR3 as shown in SEQ ID No. 1, SEQ ID No. 2, and SEQ ID No. 3, respectively; the CDR of the light chain variable region of the monoclonal antibody includes CDR1, CDR2, and CDR3 as shown in SEQ ID No. 4, SEQ ID No. 5, and SEQ ID No. 6, respectively. A sandwich ELISA method for protein content determination has been established, which is simple to operate, has good sensitivity and specificity, is widely applicable, and can perform high-throughput determination of multiple samples to detect the content of Cap protein, an effective antigen of various types of PCV2 vaccines, thus shortening the vaccine testing time.

The invention belongs to the technical field of vitamin content detection, and particularly relates to a method for measuring the content of various water-soluble vitamins B. The water-soluble vitamins B comprise vitamin B1, vitamin B2, vitamin B3, vitamin B6, vitamin B7, vitamin B12 and nicotinamide. A high performance liquid chromatograph and an electrospray detector are used as detection instruments, and chromatographic conditions are as follows: a mobile phase A is ammonium formate or ammonium acetate, a mobile phase B is methanol, and gradient elution is performed; the gradient elution procedure is as follows: a solvent of a test sample is a mixed solution of acetonitrile, water and glacial acetic acid, and the volume ratio of the acetonitrile to the water to the glacial acetic acid is 5: 95: 0.5; the atomization temperature of the electrospray detector is 35 DEG C; the method is better in separation degree and higher in specificity and sensitivity.

This invention discloses an intelligent, high-throughput method for measuring rapeseed inflorescence density in field fields and its application. The method includes: using a drone paired with an RGB camera to collect rapeseed field images at intervals throughout the entire flowering period, creating a rapeseed inflorescence dataset; employing a flower cluster (inflorescence) detection and counting strategy, using the number of detection boxes for each rapeseed flower cluster as the flower cluster count output; designing a YOLOv5 network model detection method incorporating the CBAM attention mechanism, taking into account the small target characteristics of rapeseed flower clusters; and establishing a rapeseed inflorescence count calculation model using the field dataset, followed by model testing and optimization. This invention enables rapid, accurate, and high-throughput measurement of the total number of inflorescences per unit area during the entire flowering period of rapeseed in field fields. The acquired rapeseed inflorescence density data can be used to assess the growth vigor of rapeseed in field fields and estimate rapeseed yield levels, helping to understand the growth and development status of crops such as rapeseed, and providing an important new tool for their intelligent and efficient variety breeding.

The present application relates to the technical field of phenotype measurement in aquatic genetic breeding, and particularly relates to a high-throughput phenotype measurement method and system for small-sized Macrobrachium rosenbergii. In the measurement box, a dark box environment is constructed by a white shooting bottom plate, dark light-absorbing inner walls and an LED surface array light source, combined with preview graph rough segmentation and local gray histogram analysis, to adaptively adjust the light to make the shrimp body and the background form a stable bimodal gray distribution. On this basis, a segmentation-key point joint deep learning model is used to simultaneously output the shrimp body mask and standard key points, and the topological consistency loss constraint of the skeleton side length ratio and the included angle is introduced in the training to significantly reduce the key point mislabeling and drift caused by transparent body color. The present application realizes the rapid, non-contact, high-precision and high-throughput determination of the phenotype parameters and weight of small-sized Macrobrachium rosenbergii individuals, and provides a high-quality structured phenotype database for genetic evaluation and multi-trait joint selection.

The application provides a hydrolytic nitrogen absorption device and a method for determining the content of hydrolytic nitrogen, and belongs to the technical field of analysis and detection. The hydrolytic nitrogen absorption device comprises an automatic sampling tube, a sealed tube for containing the automatic sampling tube, an elastic buckle support for fixing the automatic sampling tube on the upper part inside the sealed tube, and a sealing cover for sealing the opening at the top of the sealed tube. The device is used for pretreating the sample to be measured, and has the advantages of simple operation, high processing efficiency, high-throughput detection by using a full-automatic equipment, liberation of manpower, time and labor saving, more than 200 samples can be tested in one day (24 hours of unattended operation), low error probability, and accurate determination of the content of hydrolytic nitrogen in soil.

This invention provides the use of compounds of formula (I) as defined herein for detecting the concentration of aluminum ions in biological products such as, for example, aqueous antibody compositions.

The present invention provides methods and kits for determining telomerase processivity. These methods and kits may be used to screen for drugs that inhibit or enhance telomerase repeat addition activity, and are readily adapted to an ultra-high-throughput format.

A method for high-throughput determination of whole water toxicity, including: exposing test organisms in a wastewater sample for pollution, obtaining phenotypic feature data of the test organisms; constructing a toxicity matrix; and building a machine learning model, and in combination with the toxicity matrix, determining a whole toxicity of the wastewater sample.

This application provides a high-throughput method for measuring fruit fly feed intake and its application, belonging to the field of animal feed intake research. The method includes: transferring each of multiple fruit flies to be tested into each well of a feeding plate in a high-throughput fruit fly feeding platform; adding a liquid food with a predetermined chemical composition to each well of the food substrate of the high-throughput fruit fly feeding platform; allowing the multiple fruit flies to feed freely for a predetermined time; measuring the protein content in the remaining liquid food after free feeding to obtain the remaining protein content in each well; and obtaining the feed intake of each fruit fly based on the remaining protein content in each well. By combining a high-throughput fruit fly feeding platform with protein concentration measurement, changes in the protein content of the liquid food are tracked to estimate changes in liquid volume, thereby improving the efficiency and accuracy of fruit fly feed intake measurement while simultaneously achieving high-throughput measurement.

The present disclosure provided methods and compositions for generating a triple cell culture comprising neurons, astrocytes, and microglia. The method includes providing neurons, astrocytes, and microglial cells or precursors, and culturing them under conditions that support survival, maturation, and functional interaction. In some embodiments, the cells are derived from pluripotent stem cells and seeded in defined ratios. The cultures are suitable for modeling central nervous system function, disease processes, and for evaluating candidate therapeutic agents. Also disclosed are variations of the culture system including use of reporter genes, cryopreserved cell components, three-dimensional formats, and high-throughput assay configurations.