26 results about "High Throughput Assay" patented technology

The invention relates to the technical field of phenotype measurement in aquatic genetic breeding, in particular to a macrobrachium rosenbergii small-size shrimp high-throughput phenotype measurement method and measurement system. According to the method, a camera obscura environment is constructed through a white shooting bottom plate, a dark extinction inner wall and an LED area array light source in a measuring box body, and illumination is adaptively adjusted by combining rough segmentation of a preview and analysis of a local gray histogram, so that stable bimodal gray distribution is formed between a shrimp body and a background. On the basis, a segmentation-key point combined deep learning model is adopted, a shrimp body mask and a standard key point are output at the same time, topological consistency loss constraints of a framework side length proportion and an included angle are introduced in training, and key point mismarking and drifting caused by the transparent body color are remarkably reduced. According to the method, rapid, non-contact, high-precision and high-throughput measurement of macrobrachium rosenbergii small-specification individual phenotype parameters and weight is realized, and a high-quality structured phenotype database is provided for genetic evaluation and multi-character combined selection.

The invention belongs to the technical field of agronomy, and provides a high-throughput measurement method for the transverse diameter and the longitudinal diameter of a pineapple fruit based on image processing, which comprises the following steps: measuring the transverse diameter and the longitudinal diameter of the pineapple fruit by using a vernier caliper to obtain transverse diameter and longitudinal diameter data of the fruit, and constructing a training set and a test set; the method for automatically measuring the longitudinal stem and the transverse diameter of the pineapple fruit based on an open source computer vision library (OpenCV) is established, an efficient and accurate method is provided for high-throughput accurate evaluation of pineapple germplasm resource fruits, and accurate data support is provided for genetic analysis and gene mining of pineapple fruit size characters. And a good foundation is laid for the breeding of a new variety of the large-fruit pineapple.

This invention belongs to the field of biotechnology and relates to a specific monoclonal antibody against porcine circovirus type 2 (PCV2) Cap protein and its applications. The CDR of the heavy chain variable region of the monoclonal antibody includes CDR1, CDR2, and CDR3 as shown in SEQ ID No. 1, SEQ ID No. 2, and SEQ ID No. 3, respectively; the CDR of the light chain variable region of the monoclonal antibody includes CDR1, CDR2, and CDR3 as shown in SEQ ID No. 4, SEQ ID No. 5, and SEQ ID No. 6, respectively. A sandwich ELISA method for protein content determination has been established, which is simple to operate, has good sensitivity and specificity, is widely applicable, and can perform high-throughput determination of multiple samples to detect the content of Cap protein, an effective antigen of various types of PCV2 vaccines, thus shortening the vaccine testing time.

The invention belongs to the technical field of vitamin content detection, and particularly relates to a method for measuring the content of various water-soluble vitamins B. The water-soluble vitamins B comprise vitamin B1, vitamin B2, vitamin B3, vitamin B6, vitamin B7, vitamin B12 and nicotinamide. A high performance liquid chromatograph and an electrospray detector are used as detection instruments, and chromatographic conditions are as follows: a mobile phase A is ammonium formate or ammonium acetate, a mobile phase B is methanol, and gradient elution is performed; the gradient elution procedure is as follows: a solvent of a test sample is a mixed solution of acetonitrile, water and glacial acetic acid, and the volume ratio of the acetonitrile to the water to the glacial acetic acid is 5: 95: 0.5; the atomization temperature of the electrospray detector is 35 DEG C; the method is better in separation degree and higher in specificity and sensitivity.

An assay system permits study of cell migration through tissue-relevant extracellular matrices under a chemokine gradient within a modular assay platform. Activated CD3+ T-cells were detected, quantified, and studied as they migrated towards chemokines (CXCL10 and CXCL12) and chemokine mimetics (CXCR3 agonist) using the assay system. Discovery of drugs, and the study of microenvironments, stimuli, and responding cells are facilitated by the assay system.

This invention discloses an intelligent, high-throughput method for measuring rapeseed inflorescence density in field fields and its application. The method includes: using a drone paired with an RGB camera to collect rapeseed field images at intervals throughout the entire flowering period, creating a rapeseed inflorescence dataset; employing a flower cluster (inflorescence) detection and counting strategy, using the number of detection boxes for each rapeseed flower cluster as the flower cluster count output; designing a YOLOv5 network model detection method incorporating the CBAM attention mechanism, taking into account the small target characteristics of rapeseed flower clusters; and establishing a rapeseed inflorescence count calculation model using the field dataset, followed by model testing and optimization. This invention enables rapid, accurate, and high-throughput measurement of the total number of inflorescences per unit area during the entire flowering period of rapeseed in field fields. The acquired rapeseed inflorescence density data can be used to assess the growth vigor of rapeseed in field fields and estimate rapeseed yield levels, helping to understand the growth and development status of crops such as rapeseed, and providing an important new tool for their intelligent and efficient variety breeding.

The present application relates to the technical field of phenotype measurement in aquatic genetic breeding, and particularly relates to a high-throughput phenotype measurement method and system for small-sized Macrobrachium rosenbergii. In the measurement box, a dark box environment is constructed by a white shooting bottom plate, dark light-absorbing inner walls and an LED surface array light source, combined with preview graph rough segmentation and local gray histogram analysis, to adaptively adjust the light to make the shrimp body and the background form a stable bimodal gray distribution. On this basis, a segmentation-key point joint deep learning model is used to simultaneously output the shrimp body mask and standard key points, and the topological consistency loss constraint of the skeleton side length ratio and the included angle is introduced in the training to significantly reduce the key point mislabeling and drift caused by transparent body color. The present application realizes the rapid, non-contact, high-precision and high-throughput determination of the phenotype parameters and weight of small-sized Macrobrachium rosenbergii individuals, and provides a high-quality structured phenotype database for genetic evaluation and multi-trait joint selection.

The utility model discloses a high-flux small aquatic animal metabolic rate measuring device which comprises a test cylinder, a placing table, a breathing chamber and a breathing cup, a temperature controller is connected in the test cylinder, a main body pipeline is connected to the upper side of the placing table, a water pump is fixedly connected in the test cylinder, and a water conveying pipe is fixedly connected between the water pump and the main body pipeline; covers are arranged on the front side and the rear side of the breathing chamber, a round hole is formed in the exterior of the main body pipeline, a single-pass small pipe is fixedly connected to the exterior of the round hole, a flow stabilizing mechanism is installed in the breathing chamber, a water outlet pipe is fixedly connected to the exterior of the breathing chamber, a regulating valve is fixedly connected to the exterior of the water outlet pipe, and an oxygen dissolving instrument is installed in the breathing cup; the device can be used for measuring the metabolic rate of various aquatic invertebrates, tadpoles, fishes and other small aquatic animals, can realize high-flux measurement of a plurality of samples at the same time, has portability, is easy to assemble and carry, and can ensure the experiment precision by adjusting the water flow speeds of the pipelines to be relatively consistent.

The application provides a hydrolytic nitrogen absorption device and a method for determining the content of hydrolytic nitrogen, and belongs to the technical field of analysis and detection. The hydrolytic nitrogen absorption device comprises an automatic sampling tube, a sealed tube for containing the automatic sampling tube, an elastic buckle support for fixing the automatic sampling tube on the upper part inside the sealed tube, and a sealing cover for sealing the opening at the top of the sealed tube. The device is used for pretreating the sample to be measured, and has the advantages of simple operation, high processing efficiency, high-throughput detection by using a full-automatic equipment, liberation of manpower, time and labor saving, more than 200 samples can be tested in one day (24 hours of unattended operation), low error probability, and accurate determination of the content of hydrolytic nitrogen in soil.

The application provides a high-throughput determination and prediction method for martensite transformation starting temperature, and belongs to the technical field of steel material detection, and comprises the following steps: treating a sample; performing austenitizing treatment on the treated sample; recording the phase change process of the sample from austenite to martensite in a cooling process by using a high-temperature confocal laser scanning microscope; frame by frame analyzing the critical temperature at which each grain is first transformed into martensite, and obtaining the martensite transformation starting temperature of different grain sizes; after the sample is treated, the depth of grain boundary erosion is not less than 3 µm, and the width of grain boundary erosion is not greater than 1 µm. The HT-CLSM is used to record the martensite transformation starting temperature of different grain sizes, and in order to realize high-throughput measurement of different grain sizes and avoid mutual influence between different grains, corresponding requirements are proposed for the treatment of the sample.

This invention provides the use of compounds of formula (I) as defined herein for detecting the concentration of aluminum ions in biological products such as, for example, aqueous antibody compositions.

Disclosed herein are improved methods to assay and quantify enzymatic activity of remodeling enzymes and identifying modulators of remodeling enzyme activity. Methods for performing a nucleosome remodeling assay can comprise steps of contacting a recombinant nucleosome substrate with a remodeling enzyme; binding the recombinant nucleosome substrate to a solid support; contacting the recombinant nucleosome substrate with a cleaving enzyme that recognizes a cleaving enzyme site of the recombinant nucleosome; activating the cleaving enzyme to cleave an exposed cleaving enzyme site on the recombinant nucleosome substrate to produce a cleaved nucleosome fragment; separating the cleaved nucleosome fragment from the solid support; and quantifying an amount of DNA associated with the cleaved nucleosome fragment, wherein the amount of DNA is associated with the nucleosome remodeling activity and / or detecting histones in the cleaved nucleosome fragment.

The present invention provides methods and kits for determining telomerase processivity. These methods and kits may be used to screen for drugs that inhibit or enhance telomerase repeat addition activity, and are readily adapted to an ultra-high-throughput format.

The invention belongs to the field of plant physiological and biochemical index determination, and provides a plant multi-physiological and biochemical index high-throughput determinator based on a spectrum technology, the plant multi-physiological and biochemical index high-throughput determinator comprises a bottom plate, universal wheels with self-locking functions are arranged around the bottom of the bottom plate, and the top of the bottom plate is fixedly connected with an operation table; a servo motor in the measuring assembly drives a first bidirectional lead screw to rotate, and by means of transmission of a first chain wheel, a second chain wheel, a first chain, a second chain, a first bevel gear and a second bevel gear, a first clamping plate and a second clamping plate move along the corresponding first bidirectional lead screw, a transverse limiting rod, a second bidirectional lead screw and a vertical limiting rod; a plurality of determinator bodies are fixed, physiological and biochemical indexes of a plurality of plants can be determined at the same time, the problem that a traditional instrument cannot determine the plants at the same time is effectively solved, the determination efficiency is greatly improved, and the requirement for high-throughput determination is met; powerful support is provided for development and efficiency improvement of plant physiological and biochemical research.

3D cell cultures and devices for 3D cell culture, and methods of use thereof are provided. In some embodiments, the 3D cell culture comprise pancreatic β cells and can be generated in multi-well plates, allowing for high throughput assays on the cell culture.

Protein biosensors for drug discovery, such as a high-throughput assay utilizing TR-F to detect the properties of binding proteins (e.g., actin-binding proteins, ABPs) using a fluorescence lifetime plate reader and fluorescent probes. In particular, the present invention is a new use of TR-F technique to rapidly evaluate binding of F-actin (− or +Tm) with cMyBP-C in solution. Changes in labeled actin fluorescence lifetime due to cMyBP-C phosphorylation and / or HCM mutations correlated with binding measured by traditional cosedimentation. The present invention features methods for HTS for identifying molecules that modulate cMyBP-C or cMyBP-C-actin complex.

The invention provides a high-throughput measurement and prediction method for martensite transformation initial temperature, and belongs to the technical field of steel and iron material detection.The high-throughput measurement and prediction method comprises the steps that a sample is treated; carrying out austenitizing treatment on the treated sample; in the cooling process, a high-temperature confocal laser scanning microscope is adopted to record the phase transformation process of the sample from austenite to martensite; analyzing the critical temperature at which each grain is converted into martensite for the first time frame by frame, and obtaining the transformation initial temperature of martensite with different grain sizes; after the sample treatment, the grain boundary erosion depth is not less than 3m, and the grain boundary erosion width is not more than 1m. According to the method, the transformation starting temperatures of martensite with different grain sizes are recorded through HT-CLSM, and in order to realize high-throughput measurement of different grain sizes and avoid mutual influence among different grains, corresponding requirements are put forward for sample treatment.

A method of preparing a hydrogel array comprising a multi-well plate includes preparing a polyacrylamide (PA) hydrogel with alternating stiffnesses. Preparing the PA hydrogel includes preparing a polymer solution, plasma treating the multi-well plate, attaching a patterned mask to a plate containing the polymer solution, the plate comprising a plurality of wells, and treating the polymer solution with UV light to form the PA hydrogel, the PA hydrogel having first regions with a first stiffness and second regions with a second stiffness, the first stiffness being greater than the second stiffness.

A method and device for high-throughput determination of binding energy between ligand and protein, the method comprising: a dissociation equilibrium constant calculation step, comprising correlating soluble protein ratio and free ligand concentration under different temperature and different concentration conditions by using a ligand-protein co-precipitation theoretical model equation, to calculate the dissociation equilibrium constant at different temperatures; a binding energy parameter calculation step, comprising calculating the binding energy parameter according to the Gibbs-Helmholtz equation; and an identification step, comprising identifying the interaction type between the ligand and the protein by using the binding energy parameter. The method provided by the application can realize high-throughput determination of various binding energies between ligand and protein in complex biological samples such as cell lysate, living cells, tissues, blood and the like.

The invention provides a crystal face orientation determination method for transparent particles, which comprises the following steps of: establishing a relationship database of crystal face orientation and angular frequency by combining a traditional crystal face orientation determination method with a polarized light microscope test; when the crystal orientation information of the to-be-measured transparent particles needs to be obtained, the angular frequency is obtained through testing of the polarized light microscope, then the crystal orientation of the to-be-measured transparent particles is obtained through the angular frequency in the crystal orientation and angular frequency relation database, and then accurate and rapid measurement of the crystal orientation is achieved. According to the determination method disclosed by the invention, the rapid high-flux determination of the crystal face orientation of any micro-scale transparent particle can be realized through simple operation, and the increasing scientific research and industry requirements can be met. In addition, the determination method provided by the invention avoids damaging pretreatment such as cutting on the sample, and realizes the targets of nondestructive determination and accurate positioning.

A method for high-throughput determination of whole water toxicity, including: exposing test organisms in a wastewater sample for pollution, obtaining phenotypic feature data of the test organisms; constructing a toxicity matrix; and building a machine learning model, and in combination with the toxicity matrix, determining a whole toxicity of the wastewater sample.

Methods and compositions allow for high throughput cellular analysis across multiple parameters simultaneously. The methods allow for the mass screening and mass harvesting of data with regard to the response of large numbers of cells to a large number of drug products or other biological stimuli. Microbeads or microparticles carrying multiple biological components may be used to introduce the drug products or biological stimuli. Perturbation beads deliver a perturbation agent and a perturbation barcode, spatial-index beads deliver identifying barcodes, and capture beads capture an analyte produced by a stimulated cell. The methods allow the user to identify a specific biological response of a specific cell in a biochip or other cellular analysis vessel to a specific perturbation agent.

To provide a cell determination method, a screening method, a cell separation method, and a gel that allow determination and separation of metastatic cancer cells with high throughput.SOLUTION: A cell determination method comprises: seeding a plurality of cells on a hydrogel having a radiation-crosslinked structure of a hydrophilic polymer, which is one selected from the group consisting of a natural polymer, a derivative thereof, and an artificial protein, the hydrogel having a modulus of elasticity of 10 to 50 kPa; detecting cells adhered to the hydrogel; and estimating the cells adhered to the hydrogel as metastatic cancer cells.SELECTED DRAWING: None

The present invention includes a method for analyzing tRNAHisGTG fragments. In one aspect, the present invention includes a method of identifying a subject in need of therapeutic intervention to treat and / or prevent a disease or condition, disease recurrence, or disease progression comprises characterizing the identity of tRNAHisGTG fragments. The invention further includes diagnosing, identifying or monitoring a disease or condition, a panel of engineered oligonucleotides, a kit for a high-throughput assay, and a method and system for identifying tRNAHisGTG fragments.

This application provides a high-throughput method for measuring fruit fly feed intake and its application, belonging to the field of animal feed intake research. The method includes: transferring each of multiple fruit flies to be tested into each well of a feeding plate in a high-throughput fruit fly feeding platform; adding a liquid food with a predetermined chemical composition to each well of the food substrate of the high-throughput fruit fly feeding platform; allowing the multiple fruit flies to feed freely for a predetermined time; measuring the protein content in the remaining liquid food after free feeding to obtain the remaining protein content in each well; and obtaining the feed intake of each fruit fly based on the remaining protein content in each well. By combining a high-throughput fruit fly feeding platform with protein concentration measurement, changes in the protein content of the liquid food are tracked to estimate changes in liquid volume, thereby improving the efficiency and accuracy of fruit fly feed intake measurement while simultaneously achieving high-throughput measurement.

The present disclosure provided methods and compositions for generating a triple cell culture comprising neurons, astrocytes, and microglia. The method includes providing neurons, astrocytes, and microglial cells or precursors, and culturing them under conditions that support survival, maturation, and functional interaction. In some embodiments, the cells are derived from pluripotent stem cells and seeded in defined ratios. The cultures are suitable for modeling central nervous system function, disease processes, and for evaluating candidate therapeutic agents. Also disclosed are variations of the culture system including use of reporter genes, cryopreserved cell components, three-dimensional formats, and high-throughput assay configurations.