201results about How to "Increase the number of cells" patented technology

The present invention provides compositions including siderophore receptor polypeptides and porins from gram negative microbes, and preferably, lipopolysaccarhide at a concentration of no greater than about 10.0 endotoxin units per milliliter. The present invention also provides methods of making and methods of using such compositions.

The present invention provides compositions and methods for treating an osteochondral defect. In one embodiment, provided is a composition for treating an osteochondral defect comprising a biphasic biocompatible matrix and platelet derived growth factor (PDGF), wherein the biphasic biocompatible matrix comprises a scaffolding material and wherein the scaffolding material forms a porous structure comprising an osseous phase and a cartilage phase. In another embodiment, also provided is a method for treating an osteochondral defect in an individual comprising administering to the individual an effective amount of a composition comprising a biphasic biocompatible matrix and PDGF to at least one site of the osteochondral defect, wherein the biphasic biocompatible matrix comprises a scaffolding material and wherein the scaffolding material forms a porous structure comprising an osseous phase and a cartilage phase.

A semiconductor power device comprising a termination area that includes a trenched gate runner electrically connected to a trenched gate of said semiconductor power device. The semiconductor power device further includes a trenched field plate disposed in a trench opened in the termination area and the trenched field plate is electrically connected to the trenched gate runner. A gate runner contact trench and a field plate contact trench opened through an insulation layer covering the gate runner and the trenched field plate for extending into a doped gate dielectric filling in the trenched gate runner and the field plate wherein the gate runner contact trench and the field plate contact trench filled with a gate runner contact plug and a field plate contact plug respectively. A gate metal disposed on top of the insulation layer to electrically contact the gate runner contact plug and the field plate contact plug for electrically interconnecting the trenched gate runner and the trenched field plate.

Methods of inducing a cancer-specific immune response are disclosed through administration of an immune stimulant in the context of tumor cell death induction. Currently used clinical methods of inducing localized tumor cell death are modified to optimize immune response induction. One embodiment of the invention discloses pharmaceutical compositions and kits for modifying the palliative procedure of transarterial chemoembolization so as to promote uptake and presentation of tumor antigens in an immunostimulatory microenvironment, thereby allowing for induction of T cell, B cell and NK responses, which control not only local, but also systemic tumor growth and metastasis.

In a solar cell module in which a plurality of dye sensitized solar cells is arranged on a plane basis and is connected in series with an intercell region interposed therebetween, a first transparent substrate, a first transparent conductive film, a dye carrying oxide semiconductor layer, an electrolyte layer, a catalyst layer, a second transparent conductive film and a second transparent substrate are laminated, an insulating barrier seals cells on both sides thereof in fluid tightness and insulates them in the intercell region, an electrode connecting portion provided in a central part in a vertical direction of the insulating barrier connects an extended portion of the first transparent conductive film of one of the cells on the both sides to that of a second transparent conductive film of the other cell, and the electrode connecting portion penetrates through at least one of the first transparent substrate and the second transparent substrate in the vertical direction and is thus exposed.

The invention provides methods that enhance the production of biomass from algae that grow autotrophically, heterotrophically, or photoheterotrophically, through the use of plant growth regulators (such as growth hormones, indole acidic acid, etc.) and hormone mimics (phenoxyacetic compounds, etc.). The plant growth regulators or mimics thereof may further increase the proportion of the desired value-added products, such as biodiesel or starch, in the algae culture or the harvested biomass.

The invention relates to an extruded and foamed thermoplastic polyurethane elastomer particle and a preparation method thereof. The particle contains the following components in percentage by mass: (A) 65%-90% of a thermoplastic polyurethane elastomer, (B) 2%-30% of a thermoplastic resin polymer, (C) 0.5%-15% of a compatilizer and (D) 0.1%-5% of a foaming nucleating agent. The preparation method of a TPU formed particle comprises the following steps: uniformly mixing the thermoplastic polyurethane elastomer, the thermoplastic resin polymer, the compatilizer and the foaming nucleating agent, putting the mixture into melt extrusion equipment, and carrying out granulation, so as to obtain a blending alloy particle; and putting the blending alloy particle into foaming extrusion equipment, carrying out melting plastification so as to obtain a melt, dissolving supercritical carbon dioxide into the melt, foaming by virtue of a mouth mold, and carrying out underwater dicing, so as to obtain the formed particle. The prepared thermoplastic polyurethane foamed particle is stable in size and basically does not shrink, the surface is bright and plumy, and the diameters of foamed holes are uniform.

To provide medicinal compositions containing an active ingredient which has a low molecular weight, is free from any side effects and has an effect of potentiating nerve growth factor activity and utilization thereof for treating diseases associated with dementia, medicinal preparations containing an effective amount of vitamin K and/or its derivative showing an effect of potentiating nerve growth factor activity are provided.

As described below, the present invention features methods for reprogramming somatic cells and related therapeutic compositions and methods.

The invention provides a culture liquid for promoting ectogenesis of embryo vitrified by an open pulled straw (OPS) method after thawing. Frozen 2-cell embryo after thawing is cultured in the culture liquid containing 10<-9> mol/L of melatonin (MT), so that the blastula development rate of the embryo is improved and the number of the cells in the embryo is increased, thereby improving the embryo quality, and providing a beneficial reference for further developing the study of OPS cryopreservation of livestock embryos.

Method and apparatus for use, e.g., with Synchronous Dynamic Random Access Memory (SDRAM) circuits are disclosed. In one described embodiment, three metal layers are deposited and patterned in turn overlying a memory array portion of an SDRAM. Relatively wide power conductors are routed on a third metal layer, allowing power conductors to be reduced in size, or in some cases eliminated, on first and second metal layers. The relatively wide power conductors thus can provide a more stable power supply to the memory array, and also free some space on first and/or second metal for routing of additional and/or more widely spaced signal conductors. Other embodiments are described and claimed.

In general, this invention provides methods and compositions for stabilizing, repairing, or replacing damaged or diseased tissues or organs by engineering blood vessels in such tissues or organs. In addition, the invention further provides methods and compositions for producing functional microvascular networks useful in tissue engineering.

A method for the treatment of hepatic fibrotic injuries caused by various diseases, viral infections or toxic agents which involves the use of glatiramer acetate as an immuno-modulatory agent. The diseases to be treated are hepatic fibrosis and hepatic cellular carcinomas. Also disclose is the use of glatiramer acetate in the treatment of inflammatory bowel diseases. Additionally, disclosed are methods for screening for immuno-modulatory agents which are useful in the treatment of hepatic fibrosis, hepatic cellular carcinomas and inflammatory bowel diseases.

In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.

In semiconductor devices and methods of manufacturing semiconductor devices, a zirconium source having zirconium, carbon and nitrogen is provided onto a substrate to form an adsorption layer of the zirconium source on the substrate. A first purging process is performed to remove a non-adsorbed portion of the zirconium source. An oxidizing gas is provided onto the adsorption layer to form an oxidized adsorption layer of the zirconium source on the substrate. A second purging process is performed to remove a non-reacted portion of the oxidizing gas. A nitriding gas is provided on the oxidized adsorption layer to form a zirconium carbo-oxynitride layer on the substrate, and a third purging process is provided to remove a non-reacted portion of the nitriding gas.

The invention aims to provide an efficient culture method of CIK cells. The prepared CIK cell has the characteristics of high multiplication speed, large cell quantity, high cell viability, high killing capacity on cancer cells and the like. The efficient culture method mainly characterized in that a culture flask coated with laminin and fibronectin is used for culturing PBMCs (peripheral blood mononuclear cells), and the cells can be in a half-adherence state by means of the absorption effect of the two kinds of protein, accordingly, the cells can be stimulated by various factors, and the two kinds of protein can promote CIK cell multiplication; and with the adoption of an anti-CD28 monoclonal antibody, the co-stimulation signal of a T cell subset in the CIK cells can be stimulated, and the activation of the CIK cells is promoted. So far, a research report on jointly using laminin, fibronectin and the anti-CD28 monoclonal antibody to prepare the CIK cells is absent. The method for increasing the cell number and improving the killing activity by adding the three factors during preparation of the CIK cells is firstly provided.

The invention relates to a mammal embryo in-vitro culture device and a culture method thereof. The culture device is formed by bonding a flow channel layer and a main body layer, wherein flow channels are arranged in the flow channel layer; an electromagnetic coil is arranged below the middle part of the flow channels, and connected with a square-wave generator through a wire; the main body layer is provided with a membrane, and culture chambers are arranged around the membrane and communicated with each other through the flow channels; a magnetic sheet is arranged on the membrane and positioned above the electromagnetic coil; and the wire is provided with a circuit switch. The culture method mainly comprises the following steps: setting frequency of the square-wave generator; injecting a culture fluid and mineral oil required by embryo development into the flow channels from the culture chambers, and putting the in-vitro fertilized embryo into the culture chambers; turning on the circuit switch; and putting the whole culture device into a culture oven containing 5% of carbon dioxide to carry out culture. The invention simulates the mammal in-vivo micro environment of the embryo, stimulates the growth and development of the embryo, enhances the blastula development rate, and increases the cell count of the blastula inner cell mass.

Use of whey protein isolate in the preparation of a composition for increasing mean aerobic capacity, enhanced haemoglobin production, enhanced red blood cell production and increased mean corpuscular haemoglobin concentration in competitive species, such as equine species, involved in strenuous activity. The composition may further comprise a prebiotic such as a fructo-oligosaccharide for example inulin.