49 results about "Pasteuria" patented technology

A method of enhancing plant growth comprises the step of introducing an alkane into a location adjacent to a plant. The alkane can be introduced intermittently, and can be combined with another gas and / or nutrients. The alkane preferably comprises a butane substrate. The butane substrate can stimulate the growth of butane-utilizing bacteria, such as Aeromonas caviae, Stenotrophomonas maltophilia, Micrococcus varians, Aureobacterium esteroaromaticum, Aureobacterium barkeri, Rhodococcus fascians, Nocardia paradoxus, Comamonas acidovorans and Pseudomonas aeruginosa. The alkane can increase the amount of heterotrophic bacteria in the location adjacent to the plant, and thereby accelerate a heterotrophic nitrification process. The butane substrate can also stimulate the growth of butane-utilizing fungi. The method can also enhance the growth protists and / or prokaryotes. A system for enhancing plant growth in accordance with the method is also disclosed.

The subject invention provides a novel and advantageous strain of Pasteuria bacteria with nematicidal activity against Reniform nematodes. The subject invention provides the novel bacteria culture referred to as ATCC PTA-9643, and mutants thereof. Also provided are nematicidal compositions comprising the Pasteuria strain or its mutants or variants and methods for treating phytopathogenic and soil-dwelling nematodes.

The subject invention provides novel and advantageous methods for growing bacteria. The methods of the subject invention are particularly advantageous for growing parasitic bacteria in vitro, without the presence of host tissue. In one embodiment of the subject invention, Pasteuria endospores, such as those that infect the rootknot nematode Meloidogyne arenaria or other host nematodes, are grown in vitro under acidic conditions. The process of the subject invention is highly advantageous because Pasteuria can be grown in the absence of nematode tissue.

The invention relates to the application of a macleaya cordata extractive in veterinary drugs for economic animals, in particular to the application of the macleaya cordata extractive in the preparation of veterinary drugs with favorable inhibition effect and killing effect on common livestock and poultry diseases, such as protoblem listeria monocytogenes, haemophilus and pasteurella.

The invention discloses bacillus subtilis AP139 and fermented microbial inoculum thereof, and an application method of the bacillus subtilis AP139 and the fermented microbial inoculum. The bacillus subtilis is preserved in the China Center For Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M2014053. The bacterial strain can restrain the growth and the propagation of porcine toxigenic pasteurella multocida in a culture dish experiment by screening porcine toxigenic pasteurella multocida resistance. The bacillus subtilis AP139 is atomized in an animal breeding shed, the bacillus subtilis AP139 has an obvious characteristic of restraining the growth of pasteurella multocida in air, the pasteurella multocida attack rate in a breeding farm is reduced, at the same time, the bacillus subtilis AP139 also has a good effect on restraining escherichia coli, staphylococcus aureus and salmonella, and the bacillus subtilis AP139 has a favorable application prospect.

The subject invention provides novel and advantageous methods for growing bacteria. The methods of the subject invention are particularly advantageous for growing parasitic bacteria, in vitro, without the presence of host tissue. In one embodiment of the subject invention, Pasteuria spores, such as those that infect the rootknot nematode Meloidogyne arenaria or other host nematodes, are grown in vitro. The process of the subject invention is highly advantageous because Pasteuria can be grown in the absence of nematode tissue.

The invention discloses a method for preparing an actinobacillus pleuropneumoniae (App) bacterial ghost and a method for preparing a subunit vaccine by loading a pasteurella antigen with the App bacterial ghost. A recombinant swine App bacterial ghost is prepared by controllable double-cracking technology and a pasteurella protection gene is introduced into an App bacterial ghost carrier, so that swine pleuropneumonia and a pasteurella bigeminal gene vaccine for preventing and treating swine pasteurellosis and swine pleuropneumonia are obtained. The preparation of the bacterial ghost carrier and the application of the bacterial ghost carrier to the prevention and treatment of important animal epidemic diseases are realized and a method is provided for the research of a multi-geminal gene vaccine at the same time. An animal experiment indicates that the protection rates of the bigeminal vaccine on infectious swine pleuropneumonia and pasteurellosis are up to 99 percent and 99.2 percent respectively.

The subject invention provides novel and advantageous materials and methods for controlling phytopathogenic and / or soil-dwelling nematodes by attaching an effective amount of Pasteuria spores to a seed and delivering the seeds to the situs of nematodes.

The invention relates to a rabbit hemorrhagic disease virus baculovirus vector and pasteurella multocida bivalent inactivated vaccine and a preparation method thereof, and belongs to the field of immune technology. Recombinant rabbit hemorrhagic disease virus VP60 baculovirus is inoculated into Sf9 insect cells and cultured at 27-28 DEG C. When cell lesion reaches 85% or more, a cell culture is harvested and inactivated, and the inactivated cell culture is used as a rabbit hemorrhagic disease virus antigen. Rabbit Pasteurella multocida capsular serotype A C51-17 strain is amplified and cultured, a bacterial solution is inactivated, and the inactivated bacterial solution is used as a Pasteurella multocida antigen. The rabbit hemorrhagic disease virus baculovirus vector and pasteurella multocida bivalent inactivated vaccine can be prepared by mixing the rabbit hemorrhagic disease virus antigen and the Pasteurella multocida antigen with adjuvants in proportion. The rabbit hemorrhagic disease virus baculovirus vector and pasteurella multocida bivalent inactivated vaccine has high safety, good immune effect and simple process, and can be used for preventing and controlling Rabbit Hemorrhagic Disease (Rabbit Plague) and Rabbit Pasteurella multocida.

The invention relates to bacillus subtilis fermentation broth of a Chinese herbal medicine as well as preparation and an application of the bacillus subtilis fermentation broth. The Chinese herbal medicine refers to Chinese gall; according to pasteurella multocida and staphylococcus aureus inhibiting screening tests, the Chinese gall can inhibit growth of the pasteurella multocida and the staphylococcus aureus, bacillus subtilis AP139 is used for fermenting the Chinese gall, spraying is performed in an animal breeding shed, the fermented Chinese herbal medicine remarkably inhibits the growth of the pasteurella multocida and the staphylococcus aureus in the air, the effect is superior to those of a bacillus subtilis AP139 separate fermentation group and a Chinese herbal medicine control group, and the fermentation broth has the broader application prospect.

The invention relates to a bacilli, its compound preparation and the preparing method for the compound preparation. Its name is Bacillus subtilis LY-35, which is preserved in the general microbe center of Micro Germ conservation Management committee of China, and its conservation number is:CGMCC N0.1222. The preparation is a mixture of lambda phage of culture of bacilli and staphylococcus, streptococcus, colibacillus, bacillus proteus, pseudomonad, salmonella, pasteurella and klebsiella, can cure and prevent all kinds of illness caused by conditional pathogen,is a micro biological preparation which can be used by being mixed with antibiotics or replace antibiotics without any remainder and pollution, with good curative effect and safety.

The invention discloses a preparing and immunological efficiency analysis method of a pasteurella multocida ghost vaccine. The preparing method comprises the steps that a pasteurella multocida ghost is prepared, the induction time and the initial induced concentration are induced when the ghost splitting rate is maximum, an induced pasteurella multocida solution is centrifuged, and pasteurella multocida body sediments are collected; then the pasteurella multocida ghost vaccine and an inactivated vaccine are prepared through an aseptic PBS re-suspended pasteurella multocida body; and finally, the prepared pasteurella multocida ghost vaccine is applied to conduct immunization on a mouse. The result shows that the prepared pasteurella multocida ghost vaccine can induce the mouse to produce high-level humoral immunity and cellular immunity, and high protective power can be provided for the mouse against attack of pasteurella multocida. The preparing and immunological efficiency analysis method of the pasteurella multocida ghost vaccine lays a foundation for further study on animal pasteurella ghost vaccines.

The subject invention provides novel and advantageous methods for growing bacteria. The methods of the subject invention are particularly advantageous for growing parasitic bacteria in vitro, without the presence of host tissue. In one embodiment of the subject invention, Pasteuria endospores, such as those that infect the rootknot nematode Meloidogyne arenaria or other host nematodes, are grown in vitro under acidic conditions. The process of the subject invention is highly advantageous because Pasteuria can be grown in the absence of nematode tissue.

The subject invention provides novel and advantageous materials and methods for controlling phytopathogenic and / or soil-dwelling nematodes by attaching an effective amount of Pasteuria spores to a seed and delivering the seeds to the situs of nematodes.

The invention discloses a preparation method of a nucleic acid vaccine for fowl cholera, which comprises the following steps of: designing primers by a fowl cholera bacterium genome sequence; carrying out PCR (polymerase chain reaction) amplification by taking a fowl cholera bacterium CVCC474 strain genome as a template to obtain a target gene; carrying out enzyme digestion on the target gene and an eukaryotic expression vector pcDNA3.1(+) by utilizing a restriction enzyme KpnI and a restriction enzyme EcoRI; then connecting the target gene with the eukaryotic expression vector pcDNA3.1(+); converting into the Escherichia coli competence JM83; extracting plasmids; and carrying out enzyme digestion identification to obtain the nucleic acid vaccine for fowl cholera. The animal experiment detection shows that the nucleic acid vaccine for fowl cholera prepared by the method disclosed by the invention can reduce the occurrence of fowl cholera and can reduce the intrusion of fowl cholera bacteria on fowls; and the preparation method of the nucleic acid vaccine is simple and easy to operate.

The invention discloses an avian Pasteurella multocida gene knockout strain mediated by Ngpiwi protein and a construction method and application thereof. The avian Pasteurella multocida gene knockoutstrain is a deleted strain which deletes an ORF sequence or a partial sequence of a latent virulence-related gene on the avian Pasteurella multocida genome. Firstly, that recombinant plasmid with Ngpiwi and the left and right homologous arm of the target gene sequence to be deleted is successfully construct; secondly, the recombinant plasmid was electroporated into Pasteurella multocida and induced to double-exchange recombination at 28 DEG C, the strains deleted the target gene sequence were screened by PCR, and then the plasmid was induced to lose at 42 DEG C, thus the strains deleted the potential virulence gene sequence were obtained. The genetic operating system has the advantages of small plasmid, easy operation, wide application, no potential miss-targeting effect, high knockout efficiency and no resistance gene screening marker, which provides an excellent tool for the development of genetic engineering vaccine of avian Pasteurella multocida.

The subject invention provides novel and advantageous materials and methods for controlling phytopathogenic and / or soil-dwelling nematodes by attaching an effective amount of Pasteuria spores to a seed and delivering the seeds to the situs of nematodes.

The invention relates to applications of lactobacillin plnE. The amino acid sequence of the lactobacillin plnE is as shown in SEQ ID NO.1. The lactobacillin plnE can be used for sterilization and forimproving the immunity of weaned piglets. Compared with the prior art, a genetic engineering technology is used to perform in vitro recombination expression on the lactobacillin plnE, so that the lactobacillin plnE has strong bactericidal action against bacteria such as escherichia coli, staphylococcus aureus, salmonella and pasteurella, can be used for raising pigs or poultry, and especially canreduce diarrhea rate, improve the intestinal function and improve intestinal mucosal immunity when applied to feeds for suckling piglets, weaned piglets, growing pigs or breeding pigs.

The invention discloses a primer and probe combination for detecting pasteurella multocida in an environmental aerosol sample. The primer and probe combination is characterized by comprising a positive primer, a negative primer and a probe, wherein the sequence of the positive primer is as shown in SEQ ID NO.1, the sequence of the negative primer is as shown in SEQ ID NO.2, and the sequence of the probe is as shown in SEQ ID NO.3. The invention also discloses a kit for detecting the pasteurella multocida in the environmental aerosol sample. The content and the distribution condition of the pasteurella multocida in the environmental aerosol sample are subjected to real-time fluorescence quantitative PCR detection by using TaqMan, the occurrence risk of pasteurellosis is predicted, and reference is provided for determination of selection, use times and key disinfection areas of sanitary disinfection reagents of a field area.

The invention discloses a phoP deleted pasteurella multocida attenuated strain of birds, as well as a construction method and application thereof, and belongs to the technical field of biological engineering. The attenuated strain has a classification name of Pasteurella multocida subsp.multocida str pm0818 delta phoP, is preserved in the China Center for Type Culture Collection, has a preservation number of CCTCC NO: M2014522, and is preserved on Oct. 29, 2014. The invention further provides a method for preparing the attenuated strain. According to the method, a phoP gene is knocked out based on a suicide plasmid mediated homologous recombination method to prepare the attenuated strain. The attenuated strain has relatively low toxicity, and can be used for studying a living vaccine for preventing fowl cholera. The invention discloses application of the attenuated strain on vaccines.

The invention provides a Pasteurella multocida toxin recombinant protein, comprising three epitopes of the Pasteurella multocida toxin protein. The three epitopes have the sequences as shown in SEQ IDNO:2, 3 and 4 respectively. Also provided are a virus-like particle containing the Pasteurella multocida toxin recombinant protein, a nucleic acid sequence encoding the Pasteurella multocida toxin recombinant protein or encoding the virus-like particle containing the Pasteurella multocida toxin recombinant protein, and an immune composition of swine atrophic rhinitis comprising the Pasteurella multocida toxin recombinant protein and / or the virus-like particle containing the Pasteurella multocida toxin recombinant protein.

The invention discloses a preparation method of a nucleic acid vaccine for fowl cholera, which comprises the following steps of: designing primers by a fowl cholera bacterium genome sequence; carrying out PCR (polymerase chain reaction) amplification by taking a fowl cholera bacterium CVCC474 strain genome as a template to obtain a target gene; carrying out enzyme digestion on the target gene andan eukaryotic expression vector pcDNA3.1(+) by utilizing a restriction enzyme KpnI and a restriction enzyme EcoRI; then connecting the target gene with the eukaryotic expression vector pcDNA3.1(+); converting into the Escherichia coli competence JM83; extracting plasmids; and carrying out enzyme digestion identification to obtain the nucleic acid vaccine for fowl cholera. The animal experiment detection shows that the nucleic acid vaccine for fowl cholera prepared by the method disclosed by the invention can reduce the occurrence of fowl cholera and can reduce the intrusion of fowl cholera bacteria on fowls; and the preparation method of the nucleic acid vaccine is simple and easy to operate.

This invention describes a bacillus subtilis and the process for preparing its compound pharmaceutics. The bacillus subtilis is conserved at China General Microbiological Culture Collection Center of China Committee of Culture Collection for Microorganisms, the name is: Bacillus subtilis LY-35 and the conservation number is: CGMCC No. 1222. The pharmaceutics is a compound of the bacteriophages of such pathogens as bacillus subtilis, Staphylococcus, Streptocoaus, colibacillus, proteus, pseudomonas, salmonella, shigella, pasteurella and klebsiella. The pharmaceutics can prevent and treat the diseases caused by pathogens, and can be a non-polluted, high therapeutic effectiveness and safe substitute for antibiotics.

The invention provides a haemophilus parasuis disease and swine pasteurella multocida disease combined vaccine which comprises at least one haemophilus parasuis antigen and at least one swine pasteurella multocida antigen, preferably, the haemophilus parasuis antigen at least comprises type 4 serotype and type 5 serotype, and the swine pasteurella multocida antigen at least comprises type A capsule serotype, type B capsule serotype and type D capsule serotype. By using the combined vaccine provided by the invention, the haemophilus parasuis antigen and the swine pasteurella multocida antigen do not generate mutual interference or influence, a vaccine composition containing the haemophilus parasuis antigen and the swine pasteurella multocida antigen not only can generate protective immune response to two kinds of bacilli, but also makes the haemophilus parasuis antigen and the swine pasteurella multocida antigen generate mutually-enhanced immune effect. The combined vaccine provided by the invention has an immunization protection stage as long as one year. The invention also correspondingly provides a preparation method of the combined vaccine.

The invention discloses a duck plague and escherichia coli dual PCR diagnostic kit. The two kinds of viruses including DPV and E.coli can be simultaneously detected in the same reaction system, and the detection amount of DPV and the detection amount of E.coli are 0.2 ng / mL and 0.1 ng / mL respectively. Through sensibility is lower than that of established single virus (average) PCR, the sensibility is higher than that of a traditional serology and Elisa detection method and meets the requirement of clinical detection; it is displayed by specificity verification results for the diagnostic kit that the amplification results of duck pasteurella multocida, duck salmonella, riemerella anatipestifer and healthy duck tissue DNA are negative, which indicates that the diagnostic kit is high in specificity and is capable of fast and accurately judging single or mixed infection of DPV and E.coli in clinic samples and capable of meeting the requirement of fast detecting samples in large batches and being used and popularized in China basic layer animal prevention and control centers or breeding enterprises.

The invention discloses a preparation method for an avian pasteurella genome vaccine. The preparation method comprises the following steps of: performing enzyme digestion on a target gene by using a restrictive incision enzyme Sau3AI; performing enzyme digestion on an eucaryon expression vector PcDNA 3.1(+) by using a restrictive incision enzyme BamHI; performing dephosphorylation on the eucaryon expression vector PcDNA 3.1(+) which is subjected to enzyme digestion by using calf intestinal alkaline phosphatase; connecting an avian pasteurella genome with the eucaryon expression vector pcDNA3.1(+); transform into a colon bacillus competence JM83; extracting plasmids; and identifying through enzyme digestion to obtain the avian pasteurella genome vaccine. As proved by animal experiment detection, the avian pasteurella genome vaccine prepared with the method can be used for reducing the generation of avian pasteurellosis and reducing the invasion of avian pasteurella to birds; and the preparation method is simple and is easy to operate.

The invention provides a propolis-adjuvant inactivated vaccine for mink pasteurellosis and a method for preparing the propolis-adjuvant inactivated vaccine. The method includes: vaccinating mink original pasteurella onto a blood agar slant, culturing for 24 hours at 37 DEG C, checking purity, using as seed pasteurella liquid vaccinated blood agar matrix, inactivating to obtain an aqueous phase to be fully mixed with propolis adjuvant to obtain the propolis-adjuvant inactivated vaccine for mink pasteurellosis. The propolis-adjuvant inactivated vaccine can effectively prevent mink pasteurellosis, and adverse reactions after vaccination and influences on fur quality can be evidently reduced to create conditions for control of fur-bearing animal diseases.

The invention relates to a culture method for increasing the yield of the capsule of avian pasteurella multocida. The culture method comprises the following steps: (1) inoculation: inoculating avian pasteurella multocida into a triangular flask with a culture medium; (2) shake culture: putting the triangular flask after inoculation in the step (1) into an incubator to undergo shake culture at a temperature of 36-38 DEG C for 5.5-6.5 hours, wherein the shaker speed is 180-220rpm; (3) stationary culture: putting the triangular flask after shake culture in the step (2) into the incubator to stand, wherein the culture temperature is 36-38 DEG C and the stationary culture time is 7.5-8.5 hours. The yield of the capsule of avian pasteurella multocida can be obviously increased by adopting the culture method provided by the invention.

The invention discloses an anti-Mycoplasma bovis and Pasteurella fusion protein Md-UF1-Md-AP2 and a gene encoding the fusion protein. It induces housefly larvae with Mycoplasma pneumoniae and constructs an inhibitory subtractive library. In the inhibitory subtractive library The Md-UF1 gene screened out in the chicken-derived Salmonella housefly larvae was used to construct a suppressive subtractive library, and the Md-AP2 gene screened out in the suppressive subtractive library was constructed using the overlap extension and shearing technique. The fusion gene Md-UF1-Md-AP2, the expressed anti-Mycoplasma bovis and Pasteurella fusion protein Md-UF1-Md-AP2, has inhibitory effects on Pasteurella multocida type A and Mycoplasma bovis, The problem of drug resistance of Pasteurella bovis and Mycoplasma bovis has been solved. When the cattle have pneumonia symptoms, early medication can prevent and treat at the same time.

The present invention discloses one kind of live vaccine of fowl Pasteur's multocidum bacterium and its preparation process, and belongs to the field of biological product technology. The live vaccine is prepared with natural low virulent strain P7810 of fowl Pasteur's multocidum bacterium, and through steps of anabiosis and passage of the bacterium seed to form seed bacterium liquid, culturing to proliferate, adding gelatin in 1.5 % and cane sugar in 5 %, packing and freeze drying to obtain live vaccine of fowl Pasteur's multocidum bacterium P7810. The live vaccine is fed to fowl for immunizing to prevent fowl cholera, and has immunizing period as long as 6 months, high protecting rate, low cost and other advantages.