The invention discloses a specific primer for sex identification of
hippophae rhamnoides, an identification method and application of the specific primer, the specific primer for sex identification of
hippophae rhamnoides comprises a Higy04 primer pair or a Higy06 primer pair, the Higy04 primer pair comprises a
forward primer Higy04F: 5 '-CCCCTATAGTCACACCAACAC-3', a
reverse primer Higy04R: 5 '-ATCCCATGATCATACCATAC-3', a
reverse primer Higy04R: 5 '-ATCCCATGATATCATACCATAC-3', a
reverse primer Higy04R: 5 '- The primer pair of the Higy06 comprises a
forward primer of Higy06F: TAATCTGGTGCCTTCGATCAATT, and a reverse primer of Higy06R: TCTGTACAATTTATGCAGCACCAC, and the primer pair of the Higy06 comprises a
forward primer of Higy06F: TAATCTGGTGCCTTCGATCATT, a reverse primer of Higy06R: The invention makes an important breakthrough in the development of the sex-specific
molecular marker of the sea-buckthorn, has the remarkable advantages of high efficiency, strong universality, high accuracy, reliability and practicability, and can meet the urgent needs of breeding, cultivation and research of the sea-buckthorn.