Mammal embryo in-vitro culture device and culture method thereof

Abstract

The invention relates to a mammal embryo in-vitro culture device and a culture method thereof. The culture device is formed by bonding a flow channel layer and a main body layer, wherein flow channels are arranged in the flow channel layer; an electromagnetic coil is arranged below the middle part of the flow channels, and connected with a square-wave generator through a wire; the main body layer is provided with a membrane, and culture chambers are arranged around the membrane and communicated with each other through the flow channels; a magnetic sheet is arranged on the membrane and positioned above the electromagnetic coil; and the wire is provided with a circuit switch. The culture method mainly comprises the following steps: setting frequency of the square-wave generator; injecting a culture fluid and mineral oil required by embryo development into the flow channels from the culture chambers, and putting the in-vitro fertilized embryo into the culture chambers; turning on the circuit switch; and putting the whole culture device into a culture oven containing 5% of carbon dioxide to carry out culture. The invention simulates the mammal in-vivo micro environment of the embryo, stimulates the growth and development of the embryo, enhances the blastula development rate, and increases the cell count of the blastula inner cell mass.

Description

technical field [0001] The invention relates to the technical field of animal embryo culture, in particular to an in vitro culture device for mammalian embryos and a culture method thereof. Background technique [0002] Embryo in vitro culture technology is one of the most basic key technologies for embryo in vitro production in embryo engineering. Traditional in vitro culture devices for embryos are mainly petri dishes or plates, which can only provide a static, macroscopic, two-dimensional in vitro growth environment for embryos, which is quite different from the in vivo growth environment for embryos. Therefore, the use of this type of culture device often changes the growth rate, shape, intracellular metabolic activity and other related characteristics of the embryo, resulting in a low blastocyst development rate when the embryos fertilized in vitro are subsequently cultured in vitro using a petri dish or a culture dish , taking mice as an example, the development rate ...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide an in vitro culture device for mammalian embryos in response to the above problems, and at the same time provide a culture method using the culture device to solve the problem of in vitro ferti

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