37results about "Libraries apparatus" patented technology

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The application discloses a gene chip and a gene chip detection device. The gene chip comprises a substrate and a positioning device fixed to the upper surface of the substrate; the positioning device is provided with a containing cavity used for containing microbeads; the containing cavity is opened on the surface of the positioning device away from the substrate; and the area of the containing cavity is gradually narrowed in the direction of the height of the positioning device towards the upper surface of the substrate. The gene detection device comprises the gene chip and the microbeads covered with fluorescent probes, and the microbeads are clamped in the containing cavity. The gene chip and the gene chip detection device of the application reduce the reflection of the surface of the containing cavity of the substrate to excitation light, and at the same time, the exposed area of the microbeads is increased. In the process of using the gene chip, the proportion of the fluorescence excited by the fluorescent probes on the microbeads in the light received by the detection device is increased, so that the accuracy of the gene chip detection is improved.

To solve the problem that automation of immunoassays presents a series of challenges such as repeatability and / or reproducibility which remains goals for all automated assay systems.SOLUTION: The present invention relates to a method, a device, and a system for associating consumable data with assay consumables used in biological assays. Provided is an assay system and associated consumable, where the assay system adjusts one or more steps of an assay protocol based on consumable data specific to the consumable. Various types of the consumable data and a method of using such data in execution of assays by the assay system are described. The present invention also relates to a consumable (for example, a kit and a reagent container), software, a data deployable bundle, a computer readable medium, a loading cart, a measuring instrument, a system, and a method for performing automated biological assays.SELECTED DRAWING: Figure 2

A library preparation device is a chip including (a) a biological sample well, (b) an end repair and elution well, downstream from the biological sample well, (c) an adapter ligation and elution well, downstream from the end repair and elution well, and (d) a final library well downstream from the ligation and elution well. The library preparation device is useful in a method of library preparation on a library preparation device.

Embodiments disclosed herein are directed to microfluidic devices that allow for scalable on-chip screening of combinatorial libraries and methods of use thereof. Droplets comprising individual molecular species to be screened are loaded onto the microfluidic device. The droplets are labeled by methods known in the art, including but not limited to barcoding, such that the molecular species in each droplet can be uniquely identified. The device randomly sorts the droplets into individual microwells of an array of microwells designed to hold a certain number of individual droplets in order to derive combinations of the various molecular species. The paired droplets are then merged in parallel to form merged droplets in each microwell, thereby avoiding issues associated with single stream merging. Each microwell is then scanned, e.g., using microscopy, such as high content imaging microscopy, to detect the optical labels, thereby identifying the combination of molecular species in each microwell.

Provided herein are methods and systems of molecular profiling of diseases, such as cancer. In some embodiments, the molecular profiling can be used to identify treatments for a disease, such as treatments that were not initially identified as a treatment for the disease or not expected to be a treatment for a particular disease.

This invention relates to methods, devices, and systems for associating consumable data with assay consumables used in biological assays. [Solution] Provided are an assay system 204 and associated consumables 202, the assay system 204 adjusting one or more steps of the assay protocol based on consumable data specific to the consumables 202. Various types of consumable data and methods for using such data in performing assays with the assay system 204 are also described. The present invention also relates to consumables 202 (e.g., kits and reagent containers), software, data expandable bundles, computer-readable media, loading carts, instruments, systems, and methods for performing automated biological assays.

A system and method for querying a combinatorial synthesis library that includes a plurality of compounds, represents a plurality of reaction types, where each reaction type is mapped to a plurality of reactants, and each reactant is mapped to a plurality of synthons accepts a query in the form of a single graph into a molecular encoder model, thereby obtaining a query vector. The query vector is input into a reaction query generator model, thereby obtaining a first reaction type and a first plurality of reactants. A synthon is determined for each reactant by inputting the reactant into a synthon query generator model. Thus, a set of synthons is determined, each corresponding to a reactant among the first plurality of reactants. A molecular structure in the combinatorial synthesis library is identified that includes the set of synthons arranged according to synthesis rules associated with the first reaction type.

Provided are methods and compositions for creating an arrayed library of cells. In particular, the methods and compositions provided herein improve the selection and transfer of cells by reducing contamination, increasing throughput, and improving consistency of transfer. Such methods and compositions include automated colony selection employing a solid barrier mounted on an automated apparatus for automatic handling of one or more pick tools. The solid barrier is configured to prevent undesired transfer of cells from a pick tool into the destination receptacle. Such methods and compositions also include acoustic transfer of cells in a high-density media by an acoustic liquid handler. The methods and compositions further include pooling the cells using a bitcode sample pooling scheme for high-throughput sequencing and deconvoluting the obtained nucleic acid sequences to identify the cells from which the sequences originated.

Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.

The invention relates to the field of molecular diagnosis equipment, and discloses an NGS library preparation instrument based on a digital microfluidic technology. Comprising a rack and a top plate fixedly arranged on the rack. And the substrate is provided with an opening penetrating through the thickness direction. And the chip bearing platform is arranged in the opening and is used for bearing the digital micro-fluidic chip. And the driving plate is fixed below the substrate, is positioned below the opening area, is provided with a pin array and is in contact with a bottom electrode of the chip. The pressing and releasing mechanism comprises a first pressing and releasing part and a second pressing and releasing part, elastic abutting force is applied through an elastic assembly, so that the digital micro-fluidic chip is electrically connected with the pin array, and the abutting force can be released to release the chip. A temperature control module; and a magnetic bead control module. The preparation instrument executes multiple steps of sample treatment, temperature control, magnetic bead separation and the like in the NGS library preparation process.

To provide a method, device and system for associating consumable data with an assay consumable used for a biological assay.SOLUTION: An assay system controls one or more steps of an assay protocol on the basis of consumable data peculiar to a consumable. Even a method using various types of consumable data and data related when performing an assay by an assay system is explained. The present invention relates even to a consumable (for example, a kit and a reagent vessel) for performing an automated biological assay, software, a data expandable bundle, a computer readable medium, a loading cart, a meter, a system and a method.SELECTED DRAWING: None

Provided in the present application is a nucleic acid sequencing pretreatment workstation. In the nucleic acid sequencing pretreatment workstation, a nucleic acid extraction system is configured to controllably extract nucleic acid molecules from a biological sample; a library construction system is configured to controllably prepare the nucleic acid molecules into a library which can be used for sequencing on a machine; and a quality control system is configured to controllably measure the concentration of a nucleic acid extraction product and add a normalization reagent to the nucleic acid extraction product on the basis of the measurement result and library construction sample loading parameters, and / or controllably measure the concentration of a library construction product, add a normalization reagent to the library construction product on the basis of the measurement result, and dilute the library construction product to a concentration required for nucleic acid sequencing. By means of the cooperation of several systems, operations such as nucleic acid extraction, nucleic acid extraction product quality control, library construction, and library construction product quality control are implemented, the degree of automation of nucleic acid sequencing pretreatment is improved, and the consistency of nucleic acid sequencing is improved.

The invention discloses a gene sequencing library preparation instrument and an automatic preparation method, and relates to the technical field of gene sequencing, the gene sequencing library preparation instrument comprises a shell, a three-dimensional mechanical arm arranged in the shell, a pipetting module, a table top function module, a main rack, a plate position, a pressing piece and a control terminal arranged on the shell, the pipetting module is arranged at the tail end of the three-dimensional mechanical arm, the plate position is used for placing blocky consumables, and the pressing piece can be in magnetic attraction connection with the plate position; and in the state that the pressing piece is magnetically attracted to the plate position, the pressing piece presses and fixes the blocky consumables to the plate position. According to the gene sequencing library preparation instrument, full automation of library preparation can be achieved, preparation efficiency and preparation quality are effectively improved, and reliable data support is provided for subsequent gene sequencing.

The present invention concerns an automated substance preparation method comprising: - providing a first substance container containing a first substance; - a step of sampling to extract at least one portion of the first substance; - determination of a mass of the extracted at least one portion of the first substance; - encapsulating, in a first capsule, the extracted at least one portion of the first substance; and - repeating the step of sampling, mass determination and encapsulating to form a plurality of first capsules of the first substance having randomly distributed different masses of the first substance.

A system (100) for synthesizing copolymerized molecules on a substrate (10) includes: a light source (20) emitting exposure light; a programmable light direction unit (30) for redirecting the light in specific patterns; a substrate holder (40); a reagent delivery system (50) for dispensing reagents; and a lateral movement mechanism (60) for generating relative movement between the light direction unit and the substrate holder. A controller (70) controls: i) reagent dispensing; ii) light exposure in a first pattern; iii) relative movement and adjustment of the light direction unit to a second pattern; and iv) subsequent exposure in the second pattern.

Provided in the present invention are an automated screening platform and a screening method. The automated screening platform comprises: an automated screening device, a driving mechanism, a temperature control module and an electric control module, wherein the automated screening device is provided with a first cavity, an incubation cavity and multiple functional cavities; an injection mechanism is provided in the first cavity, and the injection mechanism comprises an injection push rod provided with a piston; a rotating module is in transmission connection with a switching mechanism, so as to control the switching mechanism to rotate; the driving mechanism can clamp the injection push rod; the temperature control module is configured to heat the incubation cavity; and the electric control module is electrically connected to the rotating module, the driving mechanism and the temperature control module, so as to control the driving mechanism and the temperature control module to work cooperatively. The automated screening platform in the present invention integrates each operation step of a screening experiment, and screening steps are all assembled inside the automated screening device, with little contact with the outside, such that external contamination and component contamination are reduced; and the entire experimental process has a high degree of automation, making experiments more standardized and unified.

The present invention relates to methods, devices and systems for associating consumable data with an assay consumable used in a biological assay. Provided are assay systems and associated consumables, wherein the assay system adjusts one or more steps of an assay protocol based on consumable data specific for that consumable. Various types of consumable data are described, as well as methods of using such data in the conduct of an assay by an assay system. The present invention also relates to consumables (e.g., kits and reagent containers), software, data deployable bundles, computer-readable media, loading carts, instruments, systems, and methods, for performing automated biological assays.

The utility model provides a library construction workstation, comprising: a nucleic acid extraction module for extracting nucleic acid; the quality inspection module is used for carrying out quality inspection on the extracted nucleic acid; the library building module and the quality inspection module are arranged side by side, and the library building module is used for carrying out library building on the nucleic acid subjected to quality inspection; the first transfer mechanism is used for transferring the nucleic acid extracted by the nucleic acid extraction module to the quality inspection module; and the second transfer mechanism is used for transferring the nucleic acid in the quality inspection module to the library building module. According to the technical scheme, through the cooperation of the nucleic acid extraction module, the quality inspection module, the library building module and the first transfer mechanism, the operations of nucleic acid extraction, quality inspection, library building, quality inspection, homogenization and the like are realized, the automation degree of library building is improved, the consistency of gene sequencing is further improved, and the influence of external risks on a detection result is reduced.

The application relates to the technical field of sequencing library preparation, in particular to a system for gene sequencing library preparation, which comprises a functional device, a cooperation device and a positioning base. The functional device comprises a sample storage device, a sample processing device and a packaging processing device. The sample storage device is used for storing a sample carrier. The sample processing device is used for transferring and distributing samples and / or sample processing reagents in the sample carrier. The packaging processing device is used for removing a protective film on the sample carrier and covering a film for the sample carrier. Each device in the functional device has a feeding and discharging interaction position. The cooperation device is used for transferring the sample carrier between the devices in the functional device. The positioning base is used for positioning the relative positions of the feeding and discharging interaction positions of the devices in the functional device, so that the cooperation device can move to the feeding and discharging interaction positions to perform feeding and discharging operations. The system helps to realize the concatenation of multiple experimental processes in sequencing library preparation, thereby improving the sample detection efficiency.

The present application provides beads bearing covalently bound compounds and covalently bound DNA barcodes, as well as methods for using such beads. The beads bear multiple copies of substantially identical compounds and multiple copies of substantially identical DNA barcodes. The compounds are composed of one or more chemical monomers, and the DNA barcodes take the form of barcode modules, each corresponding to a corresponding chemical monomer and enabling its identification. The nucleic acid barcodes can have linked or independent structures. Methods are provided for sequencing the bead-bound nucleic acid barcodes to cleave the compounds from the beads and evaluate the biological activity of the released compounds.

A streamlined nucleic acid extraction and library preparation instrument includes a frame, a carriage device, a nucleic acid extraction device, a library construction device, a purification device mounted on the frame and located on one side of the carriage device, and a storage device mounted on the frame and located on the other side of the carriage device. The storage device is adapted to the nucleic acid extraction device, the library construction device, and the purification device through the carriage device, which moves the materials in the nucleic acid extraction device, the library construction device, the purification device, and the storage device. A pipetting device is installed above the frame, which is adapted to the nucleic acid extraction device and the storage device to move the liquid in the storage device to the nucleic acid extraction device.

Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.

Examples of flow cells include substrates. Embodiments of the present disclosure also relate to methods of fabricating flow cell substrates. Some example workflows exploit light blocking properties of an imprint layer such that the process does not include etch steps. Such processes may be used to create substrates compatible with simultaneous paired-end sequencing methods.

The present invention relates in one aspect to a laboratory automation device suitable for supporting an automated and continuous processing method for identifying an anti-cancer drug or an anti-cancer drug combination for patients suffering from, or being diagnosed with, a neoplastic disease or tumor. Furthermore, a corresponding automated and continuous method for identifying an anti-cancer drug or an anti-cancer drug combination, as well as kits and corresponding uses thereof, are provided.

The present disclosure relates to systems and methods for querying a combinatorial synthesis library comprising a plurality of compounds and representing a plurality of reaction types, wherein each reaction type maps to a plurality of reactants, and each reactant maps to a plurality of synthon, accepting a query to a molecular encoder model in the form of a single graph, thereby obtaining a query vector. The query vector is input into a reaction query generator model, thereby obtaining a first reaction type and a first plurality of reactants. Synthons for each reactant are determined by inputting the reactants into a synthon query generator model. A set of synthons is thus determined, each corresponding to a reactant in the first plurality of reactants. A molecular structure in a combinatorial synthesis library is identified, the combinatorial synthesis library comprising the set of synthons arranged according to synthesis rules related to the first reaction type.

A system (100) for synthesizing copolymerized molecules on a substrate (10) includes: a light source (20) emitting exposure light; a programmable light direction unit (30) for redirecting the light in specific patterns; a substrate holder (40); a reagent delivery system (50) for dispensing reagents; and a lateral movement mechanism (60) for generating relative movement between the light direction unit and the substrate holder. A controller (70) controls: i) reagent dispensing; ii) light exposure in a first pattern; iii) relative movement and adjustment of the light direction unit to a second pattern; and iv) subsequent exposure in the second pattern.

The present invention provides an automated screening device and an automated screening platform. The automated screening device comprises a bottom plate, a flow distribution plate, a liquid storage mechanism, an injection mechanism, and a switching mechanism; a mounting hole is formed in the bottom plate; the flow distribution plate is fixed on the bottom plate, and the flow distribution plate is provided with a first flow structure and a plurality of second flow structures surrounding the first flow structure; the liquid storage mechanism is fixed on the flow distribution plate, and a first cavity, an incubation cavity and a plurality of functional cavities are formed in the liquid storage mechanism; the injection mechanism is arranged in the first cavity; the switching mechanism is arranged in the mounting hole and can rotate in the mounting hole, and the switching mechanism can rotate to a position of being in communication with the first flow structure and any second flow structure. The automated screening device of the embodiments does not require manual sample transfer, effectively reducing experimental result errors caused by manual operation; and the cavities in the liquid storage mechanism are closed spaces, reducing the risk of sample contamination.

The application discloses a library construction method, a library construction device and a library construction kit. The library construction method of one embodiment comprises the following steps: extracting a nucleic acid sample from a genetic information carrier, wherein the nucleic acid sample comprises a first sample and a second sample; adding a label to at least one of the first sample and the second sample, and merging the first sample and the second sample to establish a library; performing sequencing on the established library, and distinguishing the first sample and the second sample according to the label. The embodiment provided by the application adds a label to at least one of the first sample and the second sample after extracting the first sample and the second sample in the process of establishing a library, and distinguishes the first sample and the second sample by using the label in sequencing, so that the merging of two types of samples is realized, the library construction time is reduced, the library construction efficiency is improved, the amount of reagent consumables is reduced, and the cost is reduced.