138results about "Libraries apparatus" patented technology

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.

The invention provides an automatic screening platform and a screening method.The automatic screening platform comprises an automatic screening device, a driving mechanism, a temperature control module and an electric control module, the automatic screening device is provided with a first cavity, an incubation cavity and a plurality of functional cavities, an injection mechanism is arranged in the first cavity and comprises an injection push rod with a piston, and the injection push rod is connected with the incubation cavity; the rotating module is in transmission connection with the switching mechanism to control the switching mechanism to rotate; the driving mechanism can clamp the injection push rod; the temperature control module is used for heating the incubation cavity; the electric control module is electrically connected with the rotating module, the driving mechanism and the temperature control module so as to control the driving mechanism and the temperature control module to work cooperatively; according to the automatic screening platform, all operation steps of a screening experiment are integrated, all the screening steps are integrated in the automatic screening device, contact with the outside is little, and external pollution and assembly pollution are reduced; the whole experiment process is high in automation degree, so that the experiment is more standardized and unified.

The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.

The present invention discloses a single-cell library preparation system, including a machine base, a transfer mechanism and an extrusion mechanism, wherein a working area is formed on the machine base; the transfer mechanism includes an inlet and outlet bin movably mounted on the machine base in a horizontal direction, and the inlet and outlet bin is used for installing a microfluidic chip; the extrusion mechanism includes a multi-branch plate disposed above the working area and movably arranged in an up-down direction, and used to connect with the microfluidic chip at the working area when the multi-branch plate moves downward to the working area, so as to drive the raw materials of the microfluidic chip to flow from each raw material slot through each flow channel in sequence and then be formed and flow into the forming slot. In the technical solution of the present invention, the microfluid is transferred by the transfer mechanism, and the extrusion mechanism drives the raw materials in the multiple raw material slots of the microfluidic chip to enter the flow channel of the microfluidic chip to be formed and flow into the forming slot of the microfluidic chip, thereby realizing the forming of a single single-cell sample and the preparation of a single-cell library.

The present invention relates to methods, devices and systems for associating consumable data with analytical consumables used in biological analysis. An analysis system and related consumables are provided, wherein the analysis system adjusts one or more steps of an analysis protocol based on consumable data specifically for the consumables. Various types of consumable data are described, as well as methods for implementing analysis using the data by an analysis system. The present invention also relates to consumables (e.g., test kits and reagent containers), software, data deployment packages, computer-readable media, loading carts, instruments, systems and methods for performing automated biological analysis.

The invention provides a nucleic acid sequencing pretreatment workstation and a sequencing pretreatment method, the nucleic acid sequencing pretreatment workstation comprises: a nucleic acid extraction system configured to controllably extract nucleic acid molecules from a biological sample; the library construction system is configured to controllably prepare the nucleic acid molecules into a library capable of being used for on-machine sequencing; the quality control system is configured to controllably detect the concentration of the nucleic acid extraction product and add a homogenization reagent into the nucleic acid extraction product according to a detection result and library construction sample loading parameters; and / or, controllably performing concentration detection on the library construction product, adding a homogenization reagent into the library construction product according to a detection result, and diluting the library construction product to the concentration required by nucleic acid sequencing. According to the technical scheme, through the cooperation of the nucleic acid extraction system, the quality control system and the library construction system, the operations of nucleic acid extraction, nucleic acid extraction product substance control, library construction, library file product substance control and the like are realized, the automation degree of nucleic acid sequencing pretreatment is improved, and the consistency of nucleic acid sequencing is improved.

A parallel synthesis device for combinatorial chemistry is provided. [Solution] The parallel synthesis apparatus 100 includes a plurality of reaction vessels 102, each including a sealed chamber with a controlled gas inlet 102(a) and outlet system 102(b) for regulating the reaction environment, a reaction control module 104 connected to the reaction vessels and configured to monitor and regulate parameters including temperature, pressure, and reagent flow rates within each vessel, a reagent distribution unit 106 consisting of a plurality of precisely controlled pumps for delivering various chemical reagents to the reaction vessels, a parallel heating unit 108 integrated with each reaction vessel, a mixing device 110 installed within each reaction vessel, an automatic sampling mechanism 112 consisting of a robotic arm, and a processing unit 114 communicatively coupled to the reaction control module, the reagent distribution unit, the parallel heating unit, and the automatic sampling mechanism.

The invention provides a method for the exploration of chemical space through a multigenerational series of synthetic stages. In a first stage, a series of reactions is performed. The products of the first series are analysed, and a first product from the series of reactions is selected. The first product is used as a chemical input for each of the reactions in a second series of reactions. The products of the second series are analysed. The first and second stages are performed autonomously. The selection of a product in the first stage comprises the comparison of the products from the first series of reactions against a fitness function, where the selected product has a superior fitness compared with one or more other products in the first series. Each reaction in the first series differs in one or more chemical and / or physical inputs. Each reaction in the second series differs in one or more chemical and / or physical inputs. The invention also provides an automated exploration apparatus for performing the method, and the apparatus comprises a controller, and a chemical synthesiser and an analytical unit which are operable by the controller, as described herein.

Selectively controllable cleavable linkers include electrochemically-cleavable linkers, photolabile linkers, thermolabile linkers, chemically-labile linkers, and enzymatically-cleavable linkers. Selective cleavage of individual linkers may be controlled by changing local conditions. Local conditions may be changed by activating electrodes in proximity to the linkers, exposing the linkers to light, heating the linkers, or applying chemicals. Selective cleaving of enzymatically-cleavable linkers may be controlled by designing the sequences of different sets of the individual linkers to respond to different enzymes. Cleavable linkers may be used to attach polymers to a solid substrate. Selective cleavage of the linkers enables release of specific polymers from the solid substrate. Cleavable linkers may also be used to attach protecting groups to the ends of growing polymers. The protecting groups may be selectively removed by cleavage of the linkers to enable growth of specific polymers.

Systems and methods for querying a combinatorial synthesis library comprising a plurality of compounds and representing a plurality of reaction types, where each reaction type maps to a plurality of reactants, and each reactant maps to a plurality of synthons, accepts a query in the form of a single graph into a molecular encoder model, thereby obtaining a query vector. The query vector is inputted into a reaction query generator model thereby obtaining a first reaction type and a first plurality of reactants. A synthon is determined for each reactant by inputting the reactant into a synthon query generator model. A set of synthons is therefore determined, each corresponding to a reactant in the first plurality of reactants. A molecular structure in the combinatorial synthesis library is identified that includes the set of synthons arranged in accordance with a synthesis rule associated with the first reaction type.

The disclosure relates, inter alia, to a library of transcriptional data, and methods for assembling and using same.

The invention provides an automatic screening device and an automatic screening platform. The automatic screening device comprises a bottom plate, a valve plate, a liquid storage mechanism, an injection mechanism and a switching mechanism, and a mounting hole is formed in the bottom plate; the valve plate is fixed to the bottom plate and provided with a first flow distribution structure and a plurality of second flow distribution structures surrounding the first flow distribution structure. The liquid storage mechanism is fixed on the valve plate, and a first cavity, an incubation cavity and a plurality of functional cavities are formed in the liquid storage mechanism; the injection mechanism is arranged in the first cavity; the switching mechanism is arranged in the mounting hole and can rotate in the mounting hole, and the switching mechanism can rotate to the position where the first flow distribution structure communicates with any second flow distribution structure. According to the automatic screening device, manual sample transfer is not needed, and experimental result errors caused by manual operation are effectively reduced; and each cavity on the liquid storage mechanism is a closed space, so that the sample pollution risk is reduced.

The application discloses a gene chip and a gene chip detection device. The gene chip comprises a substrate and a positioning device fixed to the upper surface of the substrate; the positioning device is provided with a containing cavity used for containing microbeads; the containing cavity is opened on the surface of the positioning device away from the substrate; and the area of the containing cavity is gradually narrowed in the direction of the height of the positioning device towards the upper surface of the substrate. The gene detection device comprises the gene chip and the microbeads covered with fluorescent probes, and the microbeads are clamped in the containing cavity. The gene chip and the gene chip detection device of the application reduce the reflection of the surface of the containing cavity of the substrate to excitation light, and at the same time, the exposed area of the microbeads is increased. In the process of using the gene chip, the proportion of the fluorescence excited by the fluorescent probes on the microbeads in the light received by the detection device is increased, so that the accuracy of the gene chip detection is improved.

To solve the problem that automation of immunoassays presents a series of challenges such as repeatability and / or reproducibility which remains goals for all automated assay systems.SOLUTION: The present invention relates to a method, a device, and a system for associating consumable data with assay consumables used in biological assays. Provided is an assay system and associated consumable, where the assay system adjusts one or more steps of an assay protocol based on consumable data specific to the consumable. Various types of the consumable data and a method of using such data in execution of assays by the assay system are described. The present invention also relates to a consumable (for example, a kit and a reagent container), software, a data deployable bundle, a computer readable medium, a loading cart, a measuring instrument, a system, and a method for performing automated biological assays.SELECTED DRAWING: Figure 2

The present disclosure provides a barcode and print approach that links particular members of pooled sequenced libraries to specific protein variants that interact with the particular library members; and devices that allow such library-on library measurements.

Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.