124results about How to "Improve elution efficiency" patented technology

An analyte is separated from a fluid sample by introducing the sample into a cartridge having an extraction chamber containing capture material for capturing the analyte. The sample is forced to flow through the extraction chamber to capture the analyte with the capture material in the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber. The cartridge may optionally include a lysing region for lysing sample components (e.g., cells spores, or microorganisms), a waste chamber for storing waste fluid, and reaction or detection chambers for chemically reacting or detecting the eluted analyte.

A device for use with an ultrasonic transducer to lyse components of a fluid sample comprises a cartridge having a lysing chamber, an inlet port in fluid communication with the lysing chamber, and an outlet port for exit of the sample from the lysing chamber. The inlet and outlet ports are positioned to permit flow of the sample through the lysing chamber, and the chamber contains at least one solid phase for capturing the sample components to be lysed as the sample flows through the chamber. The lysing chamber is defined by at least one wall having an external surface for contacting the transducer to effect the transfer of ultrasonic energy to the chamber.

A device for lysing components (e.g., cells, spores, or microorganisms) of a fluid sample comprises a cartridge having a lysing chamber for receiving the sample and having at least one solid phase in the lysing chamber for capturing the sample components to be lysed. An ultrasonic transducer is coupled to a wall of the lysing chamber to transfer ultrasonic energy to the captured sample components.

An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.

An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.

An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.

An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.

The invention discloses multielement ex-situ remediation equipment and method used for organo-chlorine pesticide contaminated soil, belongs to the technical field of materializing remediation equipment for contaminated sites. The equipment comprises a stirring device, an eluent injecting device, an eluent recovery device, an ultrasonic generator and a heater. The equipment has extremely strong remediation broad-spectrum performance for organo-chlorine pesticide site soil with different characteristics such as organo-chlorine pesticide substance types, soil contamination incidents, soil contamination concentration, soil organic matter content, soil texture and the like, and a removal rate by single or repeated continuous elution is higher (larger than or equal to 85%); the equipment can form a good matching technology with a patent eluent (ZL201010547630.2), and is developed specially aiming at solving actual contamination situation of organo-chlorine pesticide sites in our country; and during a process of manufacturing the equipment, on the basis of synergistic effects generated by multielement strengthening components such as stirring, ultrasonic, heating and the like, the parameter condition design is optimized, the distribution is scientific and reasonable, and the optimal integration of multielement strengthening components is reflected.

The invention provides a rapid no-heating nucleic acid extraction kit based on a magnetic bead method. The kit comprises a solution containing magnetic beads, protease K, a polyA solution, deproteinizing liquid, washing liquid, eluant and lysate. The invention also provides a method for extracting nucleic acid by using the kit. The method includes the steps that the lysate and the magnetic beads are added to a sample and fully shaken for uniform mixing, and when lytic cells release nucleic acid, the nucleic acid is captured with the magnetic beads; then, the specific deproteinizing liquid andwashing liquid are adopted for washing respectively to remove protein, salt ions and other impurities on the surfaces of the magnetic beads; finally, the specific eluant is used for rapidly eluting the nucleic acid at room temperature. Compared with a conventional nucleic acid extraction kit based on a magnetic bead method, the kit has the advantages that the steps are simple and rapid, and the whole process only takes about 15 min; besides, heating is not needed, so that the probability of sample room contamination possibly caused by aerosol is reduced; no chloroform phenol or other toxic reagents are used, so that the kit is safe and free of pollution.

The invention discloses a new method for recycling gallium from a Bayer mother solution by using chelate resin. The method comprises the following steps of: (1) filling a gallium extracting resin into a resin column, and allowing a gallium-containing Bayer mother solution to flow through the resin by using an intermittent dynamic column passing method to reach adsorption saturation; (2) washing the saturated gallium extracting resin with water; (3) desorbing gallium adsorbed by the resin by using an acidic eluent, performing precipitation, impurity removal and other treatment on the eluant to obtain pure concentrated gallium-containing electrolyte, and electrolyzing to obtain the gallium; and (4) washing the eluted resin with water until the pH value of a liquid at the outlet of an exchange column is 6-8. The method has the advantages of simplicity and feasibility, high resin utilization rate, high gallium recovery rate, low energy consumption and cost, high grade of the obtained gallium product, and the like.

The present invention relates to a lectin-macromolecule carrier coupling compound for separating a glycosylated exosome in a clinical sample. The lectin-macromolecule carrier coupling compound comprises a macromolecule carrier; and lectin coupled to the outer side of the macromolecular carrier. The lectin-macromolecular carrier coupling compound provided by the invention can be used for simply, conveniently, rapidly and accurately separating the glycosylated exosomes in the clinical sample; the compound is simple in operation, high in separation efficiency and good in repeatability, and the separated exosome is complete in form and free of breakage or fragmentation and can be directly used for glycosylated exosome liquid detection or directly used for immunological related detection or directly used for gene detection and analysis by extracting related nucleic acid in the exosome.

The invention discloses a vacuum pulse type method of extracting and purifying ursolic acid of rosemary. The method comprises the following steps: (1) drying and crushing a raw material medical material rosemary; (2) carrying out vacuum pulse type reflux extraction on the material by taking 20-90% of ethanol as a solvent, and concentrating the extracting liquor for later use; and (3) carrying out ultrasonic analysis on the concentrated ursolic acid extracting liquor after being adsorbed by D101 macroporous resin, and then, concentrating, crystallizing twice and drying to obtain a 90-98% ursolic acid product. The method provided by the invention efficiently and greenly extracts ursolic acid in the whole technical process under a condition that no organic solvents except ethanol are used, and the finally obtained ursolic acid product has the activities of bacteriostatic action, tumor resistance, inflammation dimishing and the like and is wide in application prospect.

The invention discloses a method for treating high-concentration organic process wastewater generated in the production of 2-chloro-6-fluorobenzaldehyde. The method comprises the following steps: adding quicklime and flocculant into the wastewater and then regulating with acid; allowing the wastewater regulated with acid to pass through an adsorption tower filled with a nonpolar macroporous absorption resin I; respectively obtaining a primary saturated absorption resin and a primary eluent; performing desorption regeneration to the primary saturated absorption resin with a solvent I to obtaina solution after primary desorption; regulating the primary eluent with acid, and allowing the eluent regulated with acid to pass through an adsorption tower filled with a nonpolar macroporous absorption resin II; respectively obtaining a secondary saturated absorption resin and a secondary eluent; performing desorption regeneration to the secondary saturated absorption resin with a solvent II toobtain a solution after secondary desorption; and finally respectively recovering, purifying and separating the solution after primary desorption and the solution after second desorption to obtain 3-chloro-2-methylphenol, 2-chloro-6-fluorobenzaldehyde and 2-chloro-6-fluorobenzoicacid. The method can realize the organic combination of wastewater treatment and resource recovery.

The invention discloses a technical method for continuously extracting tea seed oil and saponin in tea seeds. The method comprises the following steps of: (1) tea seed treatment: drying and crushing the tea seeds; and (2) firstly, extracting the tea seed oil by using a supercritical CO2 extraction technology, then, changing the extraction temperature and pressure, adding a cosolvent, collecting secondary extracts, and separating and purifying the secondary extracts to obtain a saponin extract. All indexes of the tea seed oil obtained by using the method conform to the GB2716-2005 edible vegetable oil hygienic standard, and the mass percentage of the saponin in the saponin extract is 70-90%. The low-temperature, low-energy-consumption, green, efficient and continuous extraction of the effective ingredients in the tea seeds is realized through the effective integration of a macroporous resin absorption technology, an ultrasonic assistant extraction technology and a supercritical extraction technology; and the tea seed oil and the saponin extract are wide in purposes and market prospects.

The invention discloses a preparation method of abamectin. The preparation method comprises the following steps: (1) dissolving an abamectin crude product by adopting an organic solvent to obtain a crude product solution; (2) taking inorganic salt, a polar solvent and water and preparing an eluent with a certain concentration and eluting the crude product solution by utilizing the eluent in a counter-current extraction tower to obtain an eluting solution; (3) concentrating and drying the eluting solution, and recrystallizing a dried product to obtain a pure abamectin product. By introducing a counter-current extraction technology into an abamectin purification process, the eluting efficiency is greatly improved; large-polarity impurities can be effectively removed and continuous operation of a purification process is realized; the preparation method of the abamectin has the advantages of small equipment investment, simplicity in operation, relatively high yield, low production cost, capability of being industrially produced in a large scale and the like.

A microemulsion detergent for resource treatment of oil-containing solid waste, and a preparation method and an application thereof are provided. The microemulsion detergent comprises the components by the mass percentage: 1.2%-4.0% of fatty alcohol polyoxyethylene ether sulfate, 2.0%-4.0% of butyl lactate, 0.5%-1.0% of amyl propionate, 0.3%-0.9% of sodium chloride, and 91.1%-96% of water. The microemulsion detergent can be mixed with the oil-containing solid waste at room temperature, can complete repair treatment and can recycle oil resources with high efficiency; cheap and easily accessible NaCl is used as an electrolyte, other components have less amount, treatment operation is simple, three-phase separation is facilitated, and subsequent treatment of sludge is facilitated; and an intermediate liquid phase namely a microemulsion phase can also continue to be recycled, so that the cost is lower.

The present invention discloses a method for simultaneous detection of a plurality of content of non-protein nitrogen-containing compounds in milk by liquid chromatography-tandem mass spectrometry, the method is as follows: adding a protein precipitant into a to-be-tested sample, whirling, centrifuging, adding a degreasing agent into supernatant, whirling, centrifuging, removing a degreasing agent layer, concentrating a lower clearing liquid, dissolving with ammonia acetonitrile, whirling, performing column chromatography with a HLB solid phase extraction column, collecting an eluant, concentrating, dissolving with a liquid mixture of a 0.15% formic acid aqueous solution (containing 5mM of ammonium acetate) and acetonitrile in the volume ratio of 1: 9, and filtering to obtain a to-be-tested solution; preparing a standard mixing working solution series; respectively injecting the standard mixing working solution series and the to-be-tested solution under liquid chromatography-tandem mass spectrometry conditions, making a standard curve, and calculating the content of the non-protein nitrogen-containing compounds in the to-be-tested solution according to the standard curve. The method can be used to simultaneously detect the content of at least two of dicyclanil, dicyandiamide, biuret, cyromazine and melamine, and is high in accuracy and strong in sensitivity.

The invention discloses synthetic methods and applications of an acrylic chelated fiber APF and a color changing fiber APF-PAR. The synthetic method of the acrylic chelated fiber APF comprises the following step: the acrylic chelated fiber APF is prepared from an acrylic fiber as a parent and 1-(2-aminoethyl) piperidine as a ligand under nitrogen protection. The acrylic chelated fiber APF and PARare placed in an aqueous formaldehyde solution, the mixture is stirred and subjected to heating flux under nitrogen protection for 3-5 h, a stirring reaction ends at 70 DEG C, a product is washed withwarm water and dried to constant weight, and the color developing fiber APF-PAR is obtained. The synthesized acrylic chelated fiber APF has heavy metal ion adsorption capacity, has stronger selectiveadsorption on cupper ions and mercury ions, and is high in adsorption capacity and adsorption speed; the synthesized acrylic color developing fiber APF-PAR has sufficient tensile breaking strength tomeet actual application requirements and is applicable to detection in different environments, and color developing materials in different forms can be prepared from the fiber.

The invention discloses a selective separation and comprehensive recovery method for nickel in alkali chemical nickel waste water, and belongs to the field of heavy metal waste water treatment. The method mainly comprises the steps that 1, a I-stage large capacity adsorption and II-stage selective adsorption combined process is adopted for separating Ni in the alkali chemical nickel waste water, and effluent can be reused directly; 2, applied resin is regenerated by taking diluted acid as regenerant and adopting a cyclic preregeneration mode; 3, a high-acid high-Ni adsorption solution is mixed with an alkali chemical nickel ageing solution, Ni of multiple forms is recycled through a neutralization precipitation method or an electrocatalytic reduction method, Ni standard treatment and wash water reuse are achieved mainly through the two-stage combined process of large capacity adsorption and selective adsorption, the utilization rate of the regenerant is increased by adopting the cyclic preregeneration method, and the concentration of a desorption solution is increased; by means of 'waste control by waste' of the alkali chemical nickel ageing solution, the purposes that the strong-acidity high-concentration desorption solution is disposed at a low cost, and the nickel resource is recycled efficiently and comprehensively are achieved.

The invention discloses an eluent for a pesticide-DDT in black soil and an eluting method of the eluent. The eluent disclosed by the invention is composed of a non-ionic surfactant, an anionic surfactant, inorganic salt, an organic additive and a solvent. The specific composition of the eluent is as follows: the mass ratio of the non-ionic surfactant-twain 60 to the anionic surfactant-sodium dodecyl sulfate in the eluent is (1-3) to 1, the total concentration of the two is 3000-4000mg / L, the concentration of the inorganic salt-K2SiO3 or K2CO3 in the eluant is 1.0g / L, the concentration of the organic additive-n-propanol or n-butyl alcohol in the eluent is 1.0-2.0mol / L, and the solvent is water.

The invention relates to a low-sugar rice making method of a cooking utensil, which at least comprises the following stages: a, a starch elution stage: performing soaking for time t1 at 50-65 DEG C, performing heating to 70-90 DEG C, performing maintaining for time t2, and discharging water to separate rice from water; and b, a cooking stage: heating and cooking or steaming the rice subjected to starch elution. Compared with the prior art, the cooked rice is good in taste and low in sugar content.

The invention belongs to pollutant treatment methods in the field of environmental protection and particularly relates to a treatment method of cyanogen-containing tailing slurry. The method comprisesthe steps of positive pressure filtration, water washing, blowing-off, filtrate treatment and the like, and an integrated treatment process is formed. The method provided by the invention has the advantages that the defects of low elution efficiency, large water consumption and the like existing in traditional stirring and elution processes can be overcome, cyanides and valuable metals in the cyanogen-containing tailing slurry can be eluted and treated, harmless treatment of the cyanogen-containing tailing slurry can be realized, recovery of cyanogen and valuable metal elements in the tailingslurry can be realized, full utilization of water resources can be realized, and the method has the characteristics of high elution efficiency, low water consumption, good elution effect and broad application prospect.

The invention relates to a capture sieve. The screen mesh comprises a screen mesh framework and captured objects arranged on the screen mesh framework. The capture object can be specifically combinedwith target cells or biomolecules, wherein the screen framework is provided with metal atoms, the screen framework and the captured object are connected through a sulfhydrylation substance, the sulfhydrylation substance and the screen framework react to form a metal-sulfur bond to be combined, and the sulfhydrylation substance and the captured object react to form an amido bond to be combined; wherein the sulfhydrylation substance is one or a combination of two of sulfhydrylation sodium alginate and sulfhydrylation hyaluronic acid. According to the capturing screen, the sulfhydrylation substance is connected to the surface of the screen framework through the metal-sulfur bond. According to the invention, the sulfhydrylated substance is used as a capturing substance, the amido bond is formed between the sulfhydrylated substance and the capturing substance, the capturing substance is connected to the screen skeleton through the sulfhydrylated substance, and the sulfhydrylated sodium alginate or sulfhydrylated hyaluronic acid is adopted, so that the captured substance can be conveniently eluted subsequently on the premise of ensuring higher capturing efficiency.

The invention discloses an efficient plasma cell dissociation DNA extraction method based on a paramagnetic particle method. The efficient plasma cell dissociation DNA extraction method comprises the steps that firstly, pretreatment liquid P is prepared to preprocess a plasma sample; secondly, combination liquid B is prepared, and DNA in the sample is adsorbed through hydroxyl magnetic beads in a specific mode under the two-step temperature control condition; thirdly, washing liquid W1 and washing liquid W2 are prepared to wash the magnetic beads to which DNA is adsorbed; fourthly, efficient eluting of the sample is achieved under the auxiliary condition of physical reinforcement effect. By means of the efficient plasma cell dissociation DNA extraction method, high-quality cell dissociation DNA can be efficiently and quickly obtained, and the requirements of molecular biological experiments of PCR amplification, quantitative and qualitative analysis, sequencing and the like can be met.

A safe, simple, convenient, low-pollution and good-repeatability extracting method of alveolar lavage fluid metagenome DNAs is provided. The method includes steps of liquefying a sample, crushing cells, isolating target DNAs, removing impurity proteins and salt ions, performing DNA adsorption and performing DNA elution. The method can increase the yield and purity of the metagenome DNAs and can meet PCR, DNA library construction, gene sequencing, and other molecular biological experiments.

The invention provides a method for treating wastewater with a conductive filter material and achieving regeneration. The conductive filter material is made into a pipe shape suitable for pressure filtering, namely, a filter pipe, and the filter pipe is connected with the negative electrode of an electrolytic power supply; an inert electrode is arranged in the center of the filter pipe and connected with the positive electrode of the electrolytic power supply; one end of the filter pipe is provided with a water inlet pipe for pumping in wastewater to be treated, and the other end of the filter pipe is provided with a water outlet pipe. In the filtering process, the electrolytic power supply carries out power supply and electrolysis at the same time, or when the water outlet amount is obviously decreased after filtering is carried out for a period of time, electrolysis is started to recover the filtering performance of the filter pipe. In the electrolysis process, hydrogen bubbles are constantly generated in pore channels of the filter material serving as a negative electrode and generate huge bubble pressure in the growing process, so that particles filling the pore channels of the filter material are rapidly separated from a biological membrane, and the filtering performance of the filter material is rapidly recovered. The method solves the problems that a conventional filter material is difficult to regenerate, short in service life and the like.

The invention discloses a method for efficiently extracting longan seed polyphenols. The method comprises the following steps: (1) pulping and radiation treatment: mixing fresh longan seeds and deionized water, pulping, adding an organic acid solution, heating to 45-50 DEG C, and carrying out 60Co ionizing radiation treatment for 30-60 seconds to obtain a radiated pulp; (2) leaching and filtration treatment: adding a composite enzyme into the radiated pulp in a nitrogen protective atmosphere, extracting at 60-70 DEG C under reflux for 2-3 hours, filtering the leaching solution through an ultrafiltration membrane, and collecting the filtrate; (3) reduced pressure concentration and extraction treatment; and (4) adsorption and drying treatment. The ionizing radiation is matched with the organic acid solution to treat the pulp, thereby lowering the polyphenol binding capacity. The composite enzyme is added in the leaching process to break through the chemical bonds for binding the polyphenols, thereby enhancing the polyphenol product yield. The method is simple to operate, and has the advantages of high extraction efficiency and the like.

The invention discloses a method for detecting norovirus in a food based on negatively charged membrane concentration. The method comprises the following steps: immersing a sample to be detected in an eluant, carrying out oscillation elution at room temperature, and centrifuging the above obtained solution to obtain a supernatant; adjusting the pH value of the supernatant to 2-4, and carrying out suction filtration by using a negatively charged filter membrane with the aperture of 0.45 [mu]m to obtain suction-filtered filter paper; and shearing the suction-filtered filter paper, extracting RNA, carrying out a real-time quantitative PCR to obtain the Ct value of the sample to be detected, and finally obtaining the content of the norovirus in the sample to be detected according to a norovirus NoV GI standard curve and a norovirus NoV GII standard curve. Compared with existing local standard (DBS13 / 001-2015), the method has the advantages of time saving, high efficiency, and increase of the virus enrichment rate by 5-10%.

The method discloses a method for extracting calcium-magnesium phytate from corn soaked liquid and belongs to the technical field of starch processing. The method includes the following steps that firstly, ion exchange resin is used for adsorbing organic phosphorus in the corn soaked liquid; secondly, after ion exchange resin adsorption is saturate, the organic phosphorus on the ion exchange resin is washed and removed through a hydrochloric acid solution, and a solution containing phosphorus is obtained; thirdly, a calcium hydroxide solution is added in the solution containing phosphorus so that a neutralization reaction can be performed, and finished product liquid is obtained; fourthly, the finished product liquid is filtered and dried so that calcium phytate finished products can be obtained. Compared with the prior art, the method has the advantages that due to the fact that the organic phosphorus in the corn soaked liquid is adsorbed through the ion exchange resin, the problems that because clear limewater is directly added for a neutralization reaction in a traditional method, a large number of calcium ions exist in a solution, and an evaporator scales and cannot be used are solved, meanwhile, only the organic phosphorus in the corn soaked liquid is reduced after the corn soaked liquid passes the ion exchange resin, no other nutrient component are lost, and the method can also be used for preparing medicine, fermentation and fodder.