122results about How to "Improve elution efficiency" patented technology

An analyte is separated from a fluid sample by introducing the sample into a cartridge having an extraction chamber containing capture material for capturing the analyte. The sample is forced to flow through the extraction chamber to capture the analyte with the capture material in the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber. The cartridge may optionally include a lysing region for lysing sample components (e.g., cells spores, or microorganisms), a waste chamber for storing waste fluid, and reaction or detection chambers for chemically reacting or detecting the eluted analyte.

A device for use with an ultrasonic transducer to lyse components of a fluid sample comprises a cartridge having a lysing chamber, an inlet port in fluid communication with the lysing chamber, and an outlet port for exit of the sample from the lysing chamber. The inlet and outlet ports are positioned to permit flow of the sample through the lysing chamber, and the chamber contains at least one solid phase for capturing the sample components to be lysed as the sample flows through the chamber. The lysing chamber is defined by at least one wall having an external surface for contacting the transducer to effect the transfer of ultrasonic energy to the chamber.

The present invention provides an eluent for repairing contaminated soil. The eluent comprises, by weight, 50-70 parts of a nonionic biosurfactant and 5-30 parts of an anionic surfactant. The compounding synergism of the nonionic biosurfactant and the anionic surfactant enhances the solubilizing effect of the eluent on organic pollutants in soil, especially polycyclic aromatic hydrocarbons, so theelution efficiency of the eluent is significantly improved; and the components of the elutent are biodegradable, so the eluent does not cause secondary damage to the environment in the use process, and is an efficient, clean and environmentally-friendly in situ eluent for repairing contaminated soil. The eluent provided by the invention is firstly foamed in the use process, so the spreading efficiency of an eluent solution in the soil medium is increased, the migration of the eluent in the horizontal direction is enhanced, and the uniformity and the repairing efficiency of the eluent in the soil are further improved.

The invention provides a rapid no-heating nucleic acid extraction kit based on a magnetic bead method. The kit comprises a solution containing magnetic beads, protease K, a polyA solution, deproteinizing liquid, washing liquid, eluant and lysate. The invention also provides a method for extracting nucleic acid by using the kit. The method includes the steps that the lysate and the magnetic beads are added to a sample and fully shaken for uniform mixing, and when lytic cells release nucleic acid, the nucleic acid is captured with the magnetic beads; then, the specific deproteinizing liquid andwashing liquid are adopted for washing respectively to remove protein, salt ions and other impurities on the surfaces of the magnetic beads; finally, the specific eluant is used for rapidly eluting the nucleic acid at room temperature. Compared with a conventional nucleic acid extraction kit based on a magnetic bead method, the kit has the advantages that the steps are simple and rapid, and the whole process only takes about 15 min; besides, heating is not needed, so that the probability of sample room contamination possibly caused by aerosol is reduced; no chloroform phenol or other toxic reagents are used, so that the kit is safe and free of pollution.

The invention discloses a new method for recycling gallium from a Bayer mother solution by using chelate resin. The method comprises the following steps of: (1) filling a gallium extracting resin into a resin column, and allowing a gallium-containing Bayer mother solution to flow through the resin by using an intermittent dynamic column passing method to reach adsorption saturation; (2) washing the saturated gallium extracting resin with water; (3) desorbing gallium adsorbed by the resin by using an acidic eluent, performing precipitation, impurity removal and other treatment on the eluant to obtain pure concentrated gallium-containing electrolyte, and electrolyzing to obtain the gallium; and (4) washing the eluted resin with water until the pH value of a liquid at the outlet of an exchange column is 6-8. The method has the advantages of simplicity and feasibility, high resin utilization rate, high gallium recovery rate, low energy consumption and cost, high grade of the obtained gallium product, and the like.

The invention discloses a vacuum pulse type method of extracting and purifying ursolic acid of rosemary. The method comprises the following steps: (1) drying and crushing a raw material medical material rosemary; (2) carrying out vacuum pulse type reflux extraction on the material by taking 20-90% of ethanol as a solvent, and concentrating the extracting liquor for later use; and (3) carrying out ultrasonic analysis on the concentrated ursolic acid extracting liquor after being adsorbed by D101 macroporous resin, and then, concentrating, crystallizing twice and drying to obtain a 90-98% ursolic acid product. The method provided by the invention efficiently and greenly extracts ursolic acid in the whole technical process under a condition that no organic solvents except ethanol are used, and the finally obtained ursolic acid product has the activities of bacteriostatic action, tumor resistance, inflammation dimishing and the like and is wide in application prospect.

The invention discloses a method for treating high-concentration organic process wastewater generated in the production of 2-chloro-6-fluorobenzaldehyde. The method comprises the following steps: adding quicklime and flocculant into the wastewater and then regulating with acid; allowing the wastewater regulated with acid to pass through an adsorption tower filled with a nonpolar macroporous absorption resin I; respectively obtaining a primary saturated absorption resin and a primary eluent; performing desorption regeneration to the primary saturated absorption resin with a solvent I to obtaina solution after primary desorption; regulating the primary eluent with acid, and allowing the eluent regulated with acid to pass through an adsorption tower filled with a nonpolar macroporous absorption resin II; respectively obtaining a secondary saturated absorption resin and a secondary eluent; performing desorption regeneration to the secondary saturated absorption resin with a solvent II toobtain a solution after secondary desorption; and finally respectively recovering, purifying and separating the solution after primary desorption and the solution after second desorption to obtain 3-chloro-2-methylphenol, 2-chloro-6-fluorobenzaldehyde and 2-chloro-6-fluorobenzoicacid. The method can realize the organic combination of wastewater treatment and resource recovery.

The invention discloses a preparation method of abamectin. The preparation method comprises the following steps: (1) dissolving an abamectin crude product by adopting an organic solvent to obtain a crude product solution; (2) taking inorganic salt, a polar solvent and water and preparing an eluent with a certain concentration and eluting the crude product solution by utilizing the eluent in a counter-current extraction tower to obtain an eluting solution; (3) concentrating and drying the eluting solution, and recrystallizing a dried product to obtain a pure abamectin product. By introducing a counter-current extraction technology into an abamectin purification process, the eluting efficiency is greatly improved; large-polarity impurities can be effectively removed and continuous operation of a purification process is realized; the preparation method of the abamectin has the advantages of small equipment investment, simplicity in operation, relatively high yield, low production cost, capability of being industrially produced in a large scale and the like.

The present invention discloses a method for simultaneous detection of a plurality of content of non-protein nitrogen-containing compounds in milk by liquid chromatography-tandem mass spectrometry, the method is as follows: adding a protein precipitant into a to-be-tested sample, whirling, centrifuging, adding a degreasing agent into supernatant, whirling, centrifuging, removing a degreasing agent layer, concentrating a lower clearing liquid, dissolving with ammonia acetonitrile, whirling, performing column chromatography with a HLB solid phase extraction column, collecting an eluant, concentrating, dissolving with a liquid mixture of a 0.15% formic acid aqueous solution (containing 5mM of ammonium acetate) and acetonitrile in the volume ratio of 1: 9, and filtering to obtain a to-be-tested solution; preparing a standard mixing working solution series; respectively injecting the standard mixing working solution series and the to-be-tested solution under liquid chromatography-tandem mass spectrometry conditions, making a standard curve, and calculating the content of the non-protein nitrogen-containing compounds in the to-be-tested solution according to the standard curve. The method can be used to simultaneously detect the content of at least two of dicyclanil, dicyandiamide, biuret, cyromazine and melamine, and is high in accuracy and strong in sensitivity.

The invention discloses synthetic methods and applications of an acrylic chelated fiber APF and a color changing fiber APF-PAR. The synthetic method of the acrylic chelated fiber APF comprises the following step: the acrylic chelated fiber APF is prepared from an acrylic fiber as a parent and 1-(2-aminoethyl) piperidine as a ligand under nitrogen protection. The acrylic chelated fiber APF and PARare placed in an aqueous formaldehyde solution, the mixture is stirred and subjected to heating flux under nitrogen protection for 3-5 h, a stirring reaction ends at 70 DEG C, a product is washed withwarm water and dried to constant weight, and the color developing fiber APF-PAR is obtained. The synthesized acrylic chelated fiber APF has heavy metal ion adsorption capacity, has stronger selectiveadsorption on cupper ions and mercury ions, and is high in adsorption capacity and adsorption speed; the synthesized acrylic color developing fiber APF-PAR has sufficient tensile breaking strength tomeet actual application requirements and is applicable to detection in different environments, and color developing materials in different forms can be prepared from the fiber.

The invention discloses a selective separation and comprehensive recovery method for nickel in alkali chemical nickel waste water, and belongs to the field of heavy metal waste water treatment. The method mainly comprises the steps that 1, a I-stage large capacity adsorption and II-stage selective adsorption combined process is adopted for separating Ni in the alkali chemical nickel waste water, and effluent can be reused directly; 2, applied resin is regenerated by taking diluted acid as regenerant and adopting a cyclic preregeneration mode; 3, a high-acid high-Ni adsorption solution is mixed with an alkali chemical nickel ageing solution, Ni of multiple forms is recycled through a neutralization precipitation method or an electrocatalytic reduction method, Ni standard treatment and wash water reuse are achieved mainly through the two-stage combined process of large capacity adsorption and selective adsorption, the utilization rate of the regenerant is increased by adopting the cyclic preregeneration method, and the concentration of a desorption solution is increased; by means of 'waste control by waste' of the alkali chemical nickel ageing solution, the purposes that the strong-acidity high-concentration desorption solution is disposed at a low cost, and the nickel resource is recycled efficiently and comprehensively are achieved.

The invention relates to a low-sugar rice making method of a cooking utensil, which at least comprises the following stages: a, a starch elution stage: performing soaking for time t1 at 50-65 DEG C, performing heating to 70-90 DEG C, performing maintaining for time t2, and discharging water to separate rice from water; and b, a cooking stage: heating and cooking or steaming the rice subjected to starch elution. Compared with the prior art, the cooked rice is good in taste and low in sugar content.

The invention belongs to pollutant treatment methods in the field of environmental protection and particularly relates to a treatment method of cyanogen-containing tailing slurry. The method comprisesthe steps of positive pressure filtration, water washing, blowing-off, filtrate treatment and the like, and an integrated treatment process is formed. The method provided by the invention has the advantages that the defects of low elution efficiency, large water consumption and the like existing in traditional stirring and elution processes can be overcome, cyanides and valuable metals in the cyanogen-containing tailing slurry can be eluted and treated, harmless treatment of the cyanogen-containing tailing slurry can be realized, recovery of cyanogen and valuable metal elements in the tailingslurry can be realized, full utilization of water resources can be realized, and the method has the characteristics of high elution efficiency, low water consumption, good elution effect and broad application prospect.

The invention relates to a capture sieve. The screen mesh comprises a screen mesh framework and captured objects arranged on the screen mesh framework. The capture object can be specifically combinedwith target cells or biomolecules, wherein the screen framework is provided with metal atoms, the screen framework and the captured object are connected through a sulfhydrylation substance, the sulfhydrylation substance and the screen framework react to form a metal-sulfur bond to be combined, and the sulfhydrylation substance and the captured object react to form an amido bond to be combined; wherein the sulfhydrylation substance is one or a combination of two of sulfhydrylation sodium alginate and sulfhydrylation hyaluronic acid. According to the capturing screen, the sulfhydrylation substance is connected to the surface of the screen framework through the metal-sulfur bond. According to the invention, the sulfhydrylated substance is used as a capturing substance, the amido bond is formed between the sulfhydrylated substance and the capturing substance, the capturing substance is connected to the screen skeleton through the sulfhydrylated substance, and the sulfhydrylated sodium alginate or sulfhydrylated hyaluronic acid is adopted, so that the captured substance can be conveniently eluted subsequently on the premise of ensuring higher capturing efficiency.

The invention discloses an efficient plasma cell dissociation DNA extraction method based on a paramagnetic particle method. The efficient plasma cell dissociation DNA extraction method comprises the steps that firstly, pretreatment liquid P is prepared to preprocess a plasma sample; secondly, combination liquid B is prepared, and DNA in the sample is adsorbed through hydroxyl magnetic beads in a specific mode under the two-step temperature control condition; thirdly, washing liquid W1 and washing liquid W2 are prepared to wash the magnetic beads to which DNA is adsorbed; fourthly, efficient eluting of the sample is achieved under the auxiliary condition of physical reinforcement effect. By means of the efficient plasma cell dissociation DNA extraction method, high-quality cell dissociation DNA can be efficiently and quickly obtained, and the requirements of molecular biological experiments of PCR amplification, quantitative and qualitative analysis, sequencing and the like can be met.

A safe, simple, convenient, low-pollution and good-repeatability extracting method of alveolar lavage fluid metagenome DNAs is provided. The method includes steps of liquefying a sample, crushing cells, isolating target DNAs, removing impurity proteins and salt ions, performing DNA adsorption and performing DNA elution. The method can increase the yield and purity of the metagenome DNAs and can meet PCR, DNA library construction, gene sequencing, and other molecular biological experiments.

The invention discloses a method for efficiently extracting longan seed polyphenols. The method comprises the following steps: (1) pulping and radiation treatment: mixing fresh longan seeds and deionized water, pulping, adding an organic acid solution, heating to 45-50 DEG C, and carrying out 60Co ionizing radiation treatment for 30-60 seconds to obtain a radiated pulp; (2) leaching and filtration treatment: adding a composite enzyme into the radiated pulp in a nitrogen protective atmosphere, extracting at 60-70 DEG C under reflux for 2-3 hours, filtering the leaching solution through an ultrafiltration membrane, and collecting the filtrate; (3) reduced pressure concentration and extraction treatment; and (4) adsorption and drying treatment. The ionizing radiation is matched with the organic acid solution to treat the pulp, thereby lowering the polyphenol binding capacity. The composite enzyme is added in the leaching process to break through the chemical bonds for binding the polyphenols, thereby enhancing the polyphenol product yield. The method is simple to operate, and has the advantages of high extraction efficiency and the like.

The invention discloses a method for detecting norovirus in a food based on negatively charged membrane concentration. The method comprises the following steps: immersing a sample to be detected in an eluant, carrying out oscillation elution at room temperature, and centrifuging the above obtained solution to obtain a supernatant; adjusting the pH value of the supernatant to 2-4, and carrying out suction filtration by using a negatively charged filter membrane with the aperture of 0.45 [mu]m to obtain suction-filtered filter paper; and shearing the suction-filtered filter paper, extracting RNA, carrying out a real-time quantitative PCR to obtain the Ct value of the sample to be detected, and finally obtaining the content of the norovirus in the sample to be detected according to a norovirus NoV GI standard curve and a norovirus NoV GII standard curve. Compared with existing local standard (DBS13/001-2015), the method has the advantages of time saving, high efficiency, and increase of the virus enrichment rate by 5-10%.

An ultrasonic transmitter (95) and detector (e.g., integrated as an ultrasound transducer) utilized in a feedback control system automatically monitors and/or detects surface profile (e.g., shape) of the liquid-air interface and adjusts the flow rate of sampling liquid to ensure that experimental conditions remain consistent at the time of sample introduction during serial samplings. The feedback control can provide for automated adjustment of the surface profile of the liquid-air interface in accordance with changes in desired set point according to an experimental workflow (e.g., automated adjustment between an interface corresponding to a vortex sampling set point and an overflow cleaning set point). Improvements in desorption efficiency and quality of mass spectrometry data by degassing of the liquid solvent utilized within the sampling interfaces, and/or utilization in a feedback control system for generating data indicative of a surface profile of the liquid-air interface within the interface's sampling port may be realized.