Method for efficiently extracting longan seed polyphenols

A longan core and high-efficiency technology, applied in food extraction, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve the problems of high energy consumption, long time consumption of polyphenols, low extraction rate, etc. The effect of improving efficiency, improving purity and activity

Active Publication Date: 2017-05-31
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since cellulose, hemicellulose, and protein in plant tissues are easy to form complexes with plant polyphenols, the conventional solvent extraction of polyphenols generally has the problems of long time-consuming, high energy consumption, and low extraction rate.

Method used

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  • Method for efficiently extracting longan seed polyphenols
  • Method for efficiently extracting longan seed polyphenols

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Preliminary preparation:

[0048] Preparation of compound enzyme accelerator: mix 10g polygalacturonase, 20g pectin lyase, 5g cellulase, 10g pectin esterase, 8g Rhizopus oligosporus and 10g laccase, add 5g twelve Sodium alkylbenzene sulfonate and 80 g of purified water were used to adjust the pH value to 5.5, and shaken in a water bath at a speed of 100 r / min at 25° C. for 18 hours to obtain a compound enzyme accelerator.

[0049] Preparation of dichloromethane-chloroform mixed solution: 300 g of chemically pure grade dichloromethane solution and 100 g of chemically pure grade chloroform solution were uniformly mixed to obtain a dichloromethane-chloroform mixed solution.

[0050] The substances prepared above are used in the following method for extracting longan kernel polyphenols.

[0051] 2. A method for efficiently extracting longan kernel polyphenols, comprising the following steps:

[0052] (1) Pulping and radiation treatment: 100g of fresh longan core and 20...

Embodiment 2

[0057] 1. Preliminary preparation:

[0058] Preparation of compound enzyme accelerator: mix 11g polygalacturonase, 25g pectin lyase, 6g cellulase, 12g pectin esterase, 10g Rhizopus oligosporus and 12g laccase, add 6g twelve Sodium alkylbenzene sulfonate, 1g Tween and 85g purified water, adjust the pH value to 6, and shake in a water bath at 28°C for 19h at a speed of 120r / min to obtain a compound enzyme accelerator.

[0059] Preparation of dichloromethane-chloroform mixed solution: 330 g of chemically pure grade dichloromethane solution and 100 g of chemically pure grade chloroform solution were uniformly mixed to obtain a dichloromethane-chloroform mixed solution.

[0060] The substances prepared above are used in the following method for extracting longan kernel polyphenols.

[0061] 2. A method for efficiently extracting longan kernel polyphenols, comprising the following steps:

[0062] (1) Pulping and radiation treatment: 100g of fresh longan core and 260g deionized wat...

Embodiment 3

[0067] 1. Preliminary preparation:

[0068] Preparation of compound enzyme accelerator: mix 13g polygalacturonase, 25g pectin lyase, 7g cellulase, 12g pectin esterase, 10g Rhizopus oligospora and 11g laccase, add 5g lauric acid , 2g of coconut oil fatty acid monoethanolamide and 90g of purified water, adjust the pH value to 5.9, shake in a water bath at 30°C for 20h at a speed of 115r / min, and obtain a compound enzyme accelerator.

[0069] Preparation of dichloromethane-chloroform mixed solution: 340 g of chemically pure grade dichloromethane solution and 100 g of chemically pure grade chloroform solution were uniformly mixed to obtain a dichloromethane-chloroform mixed solution.

[0070] The substances prepared above are used in the following method for extracting longan kernel polyphenols.

[0071]2. A method for efficiently extracting longan kernel polyphenols, comprising the following steps:

[0072] (1) Pulping and radiation treatment: 100g of fresh longan core and 280g...

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Abstract

The invention discloses a method for efficiently extracting longan seed polyphenols. The method comprises the following steps: (1) pulping and radiation treatment: mixing fresh longan seeds and deionized water, pulping, adding an organic acid solution, heating to 45-50 DEG C, and carrying out 60Co ionizing radiation treatment for 30-60 seconds to obtain a radiated pulp; (2) leaching and filtration treatment: adding a composite enzyme into the radiated pulp in a nitrogen protective atmosphere, extracting at 60-70 DEG C under reflux for 2-3 hours, filtering the leaching solution through an ultrafiltration membrane, and collecting the filtrate; (3) reduced pressure concentration and extraction treatment; and (4) adsorption and drying treatment. The ionizing radiation is matched with the organic acid solution to treat the pulp, thereby lowering the polyphenol binding capacity. The composite enzyme is added in the leaching process to break through the chemical bonds for binding the polyphenols, thereby enhancing the polyphenol product yield. The method is simple to operate, and has the advantages of high extraction efficiency and the like.

Description

[0001] 【Technical field】 [0002] The invention relates to the field of deep processing of longan, in particular to a method for efficiently extracting longan kernel polyphenols. [0003] 【Background technique】 [0004] Plant polyphenols are the general term for polyhydroxy compounds, also known as tannins, which are complex secondary metabolites of phenols in plants. Plant polyphenols have relatively active hydroxyl hydrogen, which can provide hydrogen to react with free radicals to generate inert products or relatively stable free radicals, thereby interrupting or slowing down the chain reaction in which free radicals participate, so they have the effect of scavenging free radicals. It is an excellent active oxygen scavenger and lipid antioxidant, and plays a very good physiological role in the human body. In recent years, studies have found that plant polyphenols have biological activities such as prevention of high blood pressure, antithrombotic, antigastric ulcer, antivir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/77A23L33/105A61K8/9789A61Q19/00
CPCA23V2002/00A61K8/97A61K36/77A61K2236/10A61K2236/19A61K2236/33A61K2236/55A61K2800/10A61K2800/805A61K2800/81A61Q19/00A23V2200/30A23V2250/2132A23V2300/14
Inventor 何雪梅孙健李丽刘国明李昌宝郑凤锦盛金凤唐雅园李杰民零东宁
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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