Vacuum pulse type method of extracting and purifying ursolic acid of rosemary

Abstract

The invention discloses a vacuum pulse type method of extracting and purifying ursolic acid of rosemary. The method comprises the following steps: (1) drying and crushing a raw material medical material rosemary; (2) carrying out vacuum pulse type reflux extraction on the material by taking 20-90% of ethanol as a solvent, and concentrating the extracting liquor for later use; and (3) carrying out ultrasonic analysis on the concentrated ursolic acid extracting liquor after being adsorbed by D101 macroporous resin, and then, concentrating, crystallizing twice and drying to obtain a 90-98% ursolic acid product. The method provided by the invention efficiently and greenly extracts ursolic acid in the whole technical process under a condition that no organic solvents except ethanol are used, and the finally obtained ursolic acid product has the activities of bacteriostatic action, tumor resistance, inflammation dimishing and the like and is wide in application prospect.

Description

technical field [0001] The invention relates to a method for preparing ursolic acid, in particular to a method for extracting and purifying ursolic acid in rosemary by vacuum pulse. Background technique [0002] Rosemary is a perennial herb of Lamiaceae, a large cluster of evergreen shrubs, with a strong aromatic smell, and is currently recognized as a plant with a high antioxidant effect. Rosemary was introduced from the Mediterranean region of origin in 1981, and has been widely cultivated in southern my country. Rosemary is warm in nature, pungent in taste and non-toxic. It has the functions of invigorating the stomach, sweating, and calming the nerves. [0003] Ursolic acid is the main active substance in rosemary, which is a pentacyclic triterpenoid compound. Modern pharmacological studies have shown that: ursolic acid has various effects such as sedation, anti-inflammatory, antibacterial, anti-diabetic, anti-ulcer, lowering blood lipids, etc., and has obvious anti-oxi...

AI Technical Summary

Problems solved by technology

[0004] Solvent methods are mostly used to extract ursolic acid from rosemary, but conventional solvent extraction methods are difficult to guarantee high extraction efficiency. Therefore, various extraction methods such as ultrasonic-assisted, microwave-assisted, bionic extraction, and dynamic countercurrent extraction have been developed. , although these methods have improved the extraction efficiency, the production cost has not decreased significantly due to the large investment in equipment, and the prior art cannot meet the requirements of ursolic acid extraction

[0005] The reason for the low extraction efficiency of the conventional solvent method is that the main components of rosemary are substances such as cellulose, pectin and lignin, and the penetration of solvents to such substances is often poor, and ursolic acid is used as fiber Pectin, pectin and lignin are the main components of the plant cell tissue, so there is a large mass transfer resistance in the extraction process, it is difficult for the solvent to pass through the plant cell tissue to dissolve ursolic acid, and the ursolic acid is dissolved The solution is also difficult to release, the extraction time is long and the efficiency is low, and the residual rate of ursolic acid is often high

[0006] On the other hand, due to the low content of ursolic acid in rosemary, a large amount of impurities are also brought during the extraction process, so purification is required, but the purification technology at this stage has the disadvantages of low yield and high cost. Thereby, prior art can not satisfy rosemary ursolic acid extraction, purification requirement