83 results about "Dna adsorption" patented technology

The invention provides a multifunctional integrated microfluidic nucleic acid analysis chip. The chip is prepared by preparing a combination mould according to 3D printing or micromachining technology, generating a microfluidic substrate according to an injection moulding method, and completing bonding of multiple layers including a chip substrate, a liquid circuit layer, an elastic film, a gas circuit layer and the like by means of plasma cleaning to finally obtain the complete chip. The chip integrates various structures including reagent cavities, mixing cavities, a splitting cavity, a DNA (deoxyribonucleic acid) extraction cavity, amplification reaction cavities, a microvalve, a microchannel and the like and has functions of sample loading, reagent mixing, heating for splitting, DNA adsorption, cleaning, eluting, amplification reaction and the like. The multifunctional integrated microfluidic nucleic acid analysis chip has the advantages that totally-closed automatic operation of 'sample input-result output' is realized, complicated manual operations are avoided, and detection efficiency is improved.

The invention discloses a preparation method for a surface plasmon resonance (SPR) DNA sensor based on graphene oxide, and belongs to the technical field of nanometer material biology. The technical problem to be solved in the preparation method is that the distinctive effect of graphene oxide-DNA is utilized, the surface plasmon resonance technology and the signal amplification mechanism of the AuNPs are applied, based on the competitive inhibition method, the phenomenon that the single-stranded DNA with different concentrations is absorbed on the surface of a sensing chip to cause SPR spectral change, and the single-stranded DNA is detected through the linear change of the resonance angle. Through the adoption of the preparation method, the SPR technology is adopted, the GO (Graphene Oxide) is utilized to assemble the surface of the chip, the single-stranded DNA is sensitively detected through adopting the competitive inhibition method and the AuNPs signal amplification effect, the concentration of the single-stranded DNA is quantitatively detected through analyzing the change of the SPR peak, and the detection limit is ultra-low. The preparation method has the advantages as follows: the instrument and equipment are low in cost, the cost is low, the operation is simple, the efficiency is high, the precision is high, and the detection limit is ultra-low.

The invention discloses a kit and an extraction method for quickly extracting microbial genome DNA from animal fecal microorganisms. The extraction method for extracting microbial genome DNA from animal fecal microorganisms comprises the following five steps: pre-treatment of feces; splitting decomposition of microbial cells in feces; dissociation of DNA and abstraction of impure proteins; DNA adsorption by a DNA adsorption column and removal of impurities; and elution of DNA. The kit disclosed by the invention is capable of absorbing the genome DNA by a silicone mold adsorption column and removing impurities such as proteins by using special deproteinization liquid and wash liquid. Compared with conventional extraction methods for extracting microbial genome DNA from animal fecal microorganisms, the microbial genome DNA from animal fecal microorganisms can be extracted within 4 hours, so that not only is a lot of extraction time shortened, but also the experimental steps are simplified. Therefore, the microbial genome DNA from animal fecal microorganisms can be effectively, quickly and economically extracted.

A souvenir containing DNA is composed of a full-sealed container and the resin bead or glass bead in which the DNA is adsorbed. Its preparing process includes such steps as extracting DNA from a human body, adsorbing the DNA by resin bead or glass bead, loading the resin bead or glass bead in said container, sealing and vacuumizing.

The invention relates to an agent box to distill microorganism gene group DNA from earth and the distilling method. The agent box includes agent A(immersion fluid), agent B(pretreatment fluid), agent C(cracking fluid), and agent D that is made up of RNA enzyme A, agent E Al2(SO4)3, agent F hexadecyl trimethyl amine, and agent G(DNA cleaning fluid). The distilling method includes the following steps: dipping the sample, taking pretreatment to the sample, cracking the microorganism, removing humus, taking DNA coupling, removing protein impurity, adhering DNA, washing DNA, and taking elution to DNA. The advantages of the invention are rapid, little DNA loss, little harmful to researchers, high yield, and low cost, etc.

The invention provides a bacteriophage genome DNA (deoxyribonucleic acid) extraction kit and method. The kit comprises a reagent A (chloroform), a reagent B (DNase I), a reagent C (RNase A), a reagent D (precipitation buffer solution), a reagent E (pyrolysis buffer solution), a reagent F (primary pyrolysis main solution), a reagent G (secondary pyrolysis solution), a reagent H (impurity removal solution), a reagent I (DNA combination buffer solution), a reagent J (rinsing buffer solution) and a reagent K (DNA eluate). The extraction method comprises the following steps: removal of bacterium cells and fragments, degradation of bacterium nucleic acid, bacteriophage particle precipitation, bacteriophage capsid protein structure damage and hydrolysis, impurity removal, DNA liquid separation, DNA adsorption, cleaning of adsorbed DNA and elution of adsorbed DNA. The kit and method can be widely used for extracting the bacteriophage genome DNA, and has the advantages of high extraction speed, high extracted DNA yield and high purity, and the extracted DNA can not be polluted by the host bacterium genome DNA.

The invention relates to a kit and a method for quickly extracting a DNA of a plant by use of a DNA adsorption column, and belongs to the technical field of biology. The kit comprises such components as a splitting liquor, a leaching liquor, a precipitator, a deproteinizing liquor, a rinsing liquor, an eluent and the DNA adsorption column. The method comprises pretreatment of a plant tissue sample, dissociation of DNA and removal of impurity proteins, adsorption of DNA by use of the DNA adsorption column and removal of impurities, and DNA elution. The kit can be used for quickly extracting the genome DNA of the plant, and the obtained genome DNA has relatively high purity.

The invention discloses a method for quickly extracting and preserving a silica gel membrane type DNA. By utilizing the characteristic of a silica gel membrane that the silica gel membrane adsorbs DNAunder the conditions of high salt and low pH and releases the DNA under the conditions of low salt and high pH, the invention does not need a process of extracting the DNA in advance after the silicagel membrane is processed by a novel solution containing a cell cracking agent and a nuclease suppressor, cells are directly lysed, the DNA is adsorbed on the silica gel membrane, the silica gel membrane adsorbing the DNA can be preserved for a long time because of the existence of the nuclease suppressor and the degradation of the DNA is avoided. In the extracting process, the pure DNA is adsorbed on the membrane by the elution function of eluent I after salt and cell residues on the membrane are rinsed. By the elution function of eluent II, the DNA combined on the membrane is eluted into the solution. The whole DNA extracting and preserving process has quick operation and low price and is simple and convenient.

The invention relates to a method and a reagent for improving the rate of extraction of the DNAs of castoff cells in a case trace sample. Due to the problems of trace amount of case sample, keratinization of cells, multiple polymerase chain reaction (PCR) complex amplification of 16 short tandem repeat (STR) loci and the like, the extraction of the DNAs of castoff cells has long been a difficult point in forensic medicine. According to researches, the combination of pronase digestion, bath at 70 to 100 DEG C and polyethylene glycol(PEG)-ethanol magnetic bead combined system can help to effectively extract trace DNAs and to obtain DNA fragments with a proper length, so that the need of complex amplification of the 16 STR loci in case detection can be satisfied and the success rate of the case detection is improved. The pronase digestion and warm bath allow the castoff cells to release DNAs effectively and ensures the relatively complete structure of the DNAs and the easy combination of the DNAs with the magnetic beads in the PEG-ethanol system. The use of the PEG-ethanol combined system promotes the high-efficiency DNA adsorption of the magnetic beads, ensures the relatively complete structure of the DNA and ensures that DNA fragments with proper length can be obtained to satisfy the need for the complex amplification of the 16 STR loci in case detection.

The invention discloses a ribonucleic acid (RNA) extraction kit without deoxyribonucleic acid (DNA) residues and an RNA extraction method. The RNA extraction kit without the DNA residues comprises RNA eluent, DNA eluent, at least one DNA adsorption column which is matched with the RNA eluent and at least one RNA adsorption column which is matched with the DNA eluent. The RNA extraction method at least comprises the following two steps of: (A), eluting RNA on the DNA adsorption column by using the RNA eluent; and (B), eluting DNA on the RNA adsorption column by using the DNA eluent. The RNA extraction method is simple and quick; and the extracted RNA is high in purity, DNA residues are a few and cannot be seen by naked eyes under a common ultraviolet lamp.

The invention relates to a DNA extraction kit for soil urine and a method. The method comprises the following steps: 1) DNA extraction: a urine-containing soil sample is subjected to splitting decomposition with a soil lysate and proteinase K, soil and a liquid supernatant are separated centrifugally, a binding solution is added to the liquid supernatant, a silicon-based DNA adsorbing material is added after the materials are mixed, the silicon-based DNA adsorbing material and a liquid are separated, the silicon-based DNA adsorbing material is rinsed, and DNA is eluted; 2) PCR amplification and electrophoresis: DNA purified in the step 1) is subjected to PCR amplification, and DNA is detected in electrophoresis apparatus. With adoption of the kit and the method, DNA adsorption by silicon dioxide sourced from sample soil is eliminated, so that unnecessary DNA loss is reduced, and high-quality and high-yield DNA from soil urine can be obtained.

This invention relates to a biological sample DNA extraction method, which comprises the following steps: a, leading the micro magnetic leads with carboxyl mark into the micro channel of the chip and absorbing and fixing it inside the channel to form the micro flow chip of the sample; b, mixing the sample and DNA absorbing liquid and then leading into the chip and mixing it with the micro magnetic leads, wherein the DNA in sample is absorbed on the leads with buffer liquid flowing in for washing and then collecting the washing liquid to get the high purified DNA.

The invention provides a kit for rapidly extracting genomes of animal excrement by virtue of a CTAB method. The kit is utilized for carrying out pretreating the excrement, splitting microbial cells in the excrement, dissociating DNA, abstracting impurity protein, adsorbing DNA by virtue of a DNA adsorption column, removing impurities and eluting DNA; after cells are disrupted, protein impurities are removed by virtue of a CTAB solution, meanwhile, genome DNA is adsorbed by virtue of a silicone mold adsorption column, and finally, the residual protein impurities are eluted by virtue of special deproteinization liquid and rinsing liquid. A conventional animal excrement microbial genome extraction method needs to be finished within 14 hours or longer, and the genomes are easily degraded due to the long-playing operation. Compared with the conventional animal excrement microbial genome extraction method, the kit has the advantages that the animal excrement genomes can be extracted within only 4 hours, so that plenty of extraction time is saved, meanwhile, experimental steps are simplified, and the microbial genomes of the animal excrement can be relatively effectively, rapidly and economically extracted.

A safe, simple, convenient, low-pollution and good-repeatability extracting method of alveolar lavage fluid metagenome DNAs is provided. The method includes steps of liquefying a sample, crushing cells, isolating target DNAs, removing impurity proteins and salt ions, performing DNA adsorption and performing DNA elution. The method can increase the yield and purity of the metagenome DNAs and can meet PCR, DNA library construction, gene sequencing, and other molecular biological experiments.

The invention relates to a kit for extracting fungal genome DNA and belongs to the technical field of biology. The kit for extracting the fungal genome DNA comprises the specific steps of crushing fungal cells, dissociating DNA and removing impure proteins, adsorbing DNA by using a DNA adsorbing column, removing impurities and eluting DNA, finally, so as to obtain a liquid which is the fungal genome DNA which is subjected to electrophoresis detection in 1% sepharose gel. The conventional steps for extracting the fungal genome DNA are tedious, consume a long time, and can extract the fungal genome DNA in about 18 hours generally. The method provided by the invention, compared with the conventional fungal genome extraction method, only needs about 7 hours to extract the fungal genome DNA, so that a lot of extraction time is saved, and the fungal genome DNA can be extracted more effectively, quickly and economically. Meanwhile, the kit is suitable for commercial production and has a certain economic value.

The invention provides a batch and fast DNA extraction method and extraction device, and relates to the technical field of a biological device. DNA extraction liquid is added into a deep hole plate; an extraction device is pressed for several seconds; filter paper falls into the extraction liquid; after 3 to 5 seconds, DNA is adsorbed onto the filter paper. The device comprises a press plate, a filter paper groove plate, a protection pipe and elastic posts uniformly distributed on the press plate at equal intervals; the quantity and the space of the elastic posts are identical to the quantityand spacing of holes on the deep hole plate; the head parts of the elastic posts are fixed at one side of the press plate; the filter paper is clamped in the lower end longitudinal center shaft position of the clamping part at the other end; the clamping part is inserted into a downward extending protection pipe of the filter paper groove plate; a spring is sleeved on the outer periphery of the head of the elastic post, so that a certain distance is formed between the filter paper groove plate and the press plate; a slide resistance block for preventing the clamping part from sliding out extends in the axis direction from the top end of the protection pipe; anti-slip bulges corresponding to the slide resistance blocks are arranged on the outer periphery of the clamping part. The method andthe device have the beneficial effects that the fast and batch DNA adsorption is realized; the adhesion between adjacent pieces of filter paper can be prevented; the sample crossed pollution is prevented; the operation is simple; the application range is wide.

The invention discloses preparation of a graphene-based composite material and application thereof in chemiluminiscence detecting the DNA (Deoxyribonucleic Acid) content. The main technical characteristic is characterized in that according to a method, magnetism is modified on the surface of graphene oxide, and a magnetic graphite base composite material with a conjugate adsorption capacity on a single-stranded DNA molecule is synthetized, the preparation process is simple, the conditions are easy to control, and the production cost is low. The invention provides a new method for detecting DNA. The synthetized Fe3O4@SiO2/GO composite material can realize quick adsorption on the single-stranded DNA, is strong in adsorption capacity, and high in efficiency; the time for the single-stranded DNA adsorption to achieve the balance is only 10 to 15min, and the maximal adsorption capacity is the saturated adsorption amount achieving the magnitude order 10<-7> to 10 <-6>mol/g. The method for detecting DNA is low in detection line, can achieve 10<-9>mol/L, and has the advantages of high sensitivity, high selectivity, good reproducibility, no pollution on the environment and the like.

The invention discloses a total DNA extraction method for Eremochloa ophiuroides (Munro.) Hack. and relates to the technology of extracting total DNA from plants in the field of molecular biology. The total DNA extraction method mainly comprises the following steps: adding a cell nucleus extraction buffer solution into Eremochloa ophiuroides (Munro.) Hack. leaves which are ground into fine powder, and achieving DNA adsorption and elution and DNA purification and separation through silicon beads. According to the invention, the silicon beads are used for effectively adsorbing DNA, removing secondary metabolism products, such as polysaccharose, polyphenols, pigment and protein, and extracting to obtain high-quality Eremochloa ophiuroides (Munro.) Hack. total DNA. The total DNA extraction method can extract high-quality Eremochloa ophiuroides (Munro.) Hack. total DNA from Eremochloa ophiuroides (Munro.) Hack. tissues preserved at low temperature and fresh Eremochloa ophiuroides (Munro.) Hack. tissues, and is suitable for extracting high-quality total DNA from Eremochloa ophiuroides (Munro.) Hack.

The invention relates to a DNA preservation method and equipment, in particular to a DNA specimen normal temperature preservation preparation method and packaging equipment. The DNA specimen normal temperature preservation preparation method comprises the following steps: enabling DNA to be adsorbed on an adsorption film, taking out the adsorption film, placing the adsorption film in a 3ML disinfection clean specimen bottle, then placing the specimen bottle on a DNA normal temperature preservation automatic packaging machine, and placing the DNA normal temperature preservation automatic packaging machine in a vacuum drying box chamber; carrying out first vacuumizing on the vacuum drying box chamber to 0.005MPa, raising the temperature to 10-35 DEG C, and drying for 30mins; filling nitrogen into the vacuum drying box chamber until the pressure is 0.1MPa; carrying out second vacuumizing to 0.005MPa; filling the nitrogen the vacuum drying box chamber secondly until the pressure is 0.1MPa; carrying out third vacuumizing to 0.005MPa; automatically plugging a rubber plug into a bottle mouth of the specimen bottle by the DNA normal temperature preservation automatic packaging machine; and taking out the DNA normal temperature preservation automatic packaging machine and the specimen bottle, adding a metal cover cap at the external of the specimen bottle, and permanently packaging the external of the metal cover cap and the neck of the specimen bottle together with resin. According to the method, harmful gas in the packaging environment is greatly lowered, the DNA is prevented from being damaged by high temperature, and automatic packaging is finished in the vacuum environment.

The invention discloses a real-time fluorescent quantitative PCR (polymerase chain reaction) detection kit for shrimp white spot syndrome virus. The kit comprises the following reagents of a reagent A, a reagent B, a reagent C, a reagent D, a reagent E, a reagent F, a reagent G, sterilized double distilled water, and a DNA (deoxyribonucleic acid) absorption column, wherein the reagent A is prepared by adding water into hexadecyl trimethyl ammonium bromide, NaCl (sodium chloride), ethylene diamine tetraacetate, and hydroxymethyl aminomethane-hydrochloric acid, the reagent B adopts protease K, a solvent of the reagent B is the sterilized double distilled water, the reagent C is an ethanol water solution, the reagent D is prepared by mixing a PCR reaction buffer containing MgCl2 (magnesium chloride), Taq (thermos aquaticus) DNA polymerase and the sterilized double distilled water, the reagent E is prepared by mixing a primer P1, a primer P2 and deoxyribonucleotide, the reagent F is a fluorescent probe premixed solution, and the reagent G adopts a shrimp white spot syndrome virus positive plasmid. The kit has the advantages that the detection sensitivity is high, the kit is used for the early infection and tracking monitoring of the cultivation shrimp white spot syndrome virus, and the kit is also used for the related basic scientific research of virus multiplication.

The invention provides a kit for extracting a gram-positive bacterium genome, and belongs to the field of biotechnology. The kit comprises the following specific steps: breaking gram-positive bacterium cells, dissociating DNA and removing impurity protein, adsorbing the DNA by virtue of a DNA adsorption column and removing impurities, and eluting the DNA. Different from a conventional kit for extracting a gram-positive bacterium genome which is relatively complex in extraction step and relatively long in extraction time consumed which generally requires about 12h, the kit for extracting the gram-positive bacterium genome, compared with a conventional kit in method for extracting gram-positive bacterium genome DNA, is capable of extracting the gram-positive bacterium genome DNA for only about 2h, so as to save a lot of extraction time; and meanwhile, the kit can simplify experimental steps, and can be used for extracting the gram-positive bacterium genome DNA more effectively, rapidly and economically.

The invention relates to a kit and a method for extracting pectin-rich polysaccharide plant DNA. The kit comprises a desaccharifying buffer solution, a lysate, leaching liquor, a column buffer solution, a protein-free solution, a rinsing solution, an eluant and a DNA adsorption column. An extraction method comprises the following steps: removing polysaccharide from a plant, extracting DNA, subsequently extracting and removing polysaccharide again by filtering with a column, thereby acquiring the extracted DNA with high quality and no impurity. The kit and the method provided by the invention are suitable for the extraction of plant genome DNA of mass secondary metabolites rich in polysaccharide, polyphenol, and the like. The kit has the characteristics of a low cost, simple, convenient andquick operation, high quality, high efficiency, high applicability, and the like.

The invention discloses a kit and method for extracting collybia radicata genome DNA (Deoxyribonucleic Acid). The kit is prepared from the following components: an equilibrium liquid including 1 percent of Na2SiO3.9H2O and 1 percent of NaOH, lysate including 5.0M of guanidine hydrochloride, 25 percent of isopropanol and 1 percent of PVP K30, a cleaning solution including 20 mM of NaCl, 20 mM of Tris-HCl and 85 percent of ethanol with pH of 7.5, eluent including 10 mM of Tris-HCl with the pH of 8.5 and a DNA adsorption column. The reagent for extracting the collybia radicata genome DNA does notcontain toxic substances such as phenol and chloroform, so that higher safety is realized; the extraction method is simple and efficient and the extraction time is shortened to 15 minutes; an extracted product has high purity and good integrity, and can be directly used for molecular biology and genetic research.

The invention relates to a method for recycling DNA from agarose gel. The method for recycling DNA samples from agarose gel after electrophoresis comprises the following steps in sequence: first, stripped absorbent material of wedge type is prepared, then inserted into gel, sucks out the DNA samples, and then the method is finished through centrifugal collection, DNA absorption, DNA washing, DNA elution, etc; the invention overcomes the defects existing in the prior art of complicated operation of cutting gel for recycling and easy cross contamination when operating a plurality of samples, provides a method for recycling liner or annular DNA from agarose gel after electrophoresis, can recycle DNA segments for purification from agarose gel within 15min, has high pureness and can meet the subsequent operation of molecular cloning, enzyme analysis, marking, PCR, etc.; the method adopts easy equipment, has convenient operation, high recycling rate and low cost and can put reagent in kits.