10 results about "Dna adsorption" patented technology

According to the decalcification-free trace DNA extraction method for the old bone sample, an independent decalcification step is not adopted, a specific metal chelating agent and a phosphoramidate derivative are introduced into a bone incubation solution, the specific metal chelating agent has high chelating capacity on calcium and magnesium ions, and the specific metal chelating agent and a complex product thereof are high in water solubility and not prone to being separated out; the phosphoramidate derivative is easy to remove and does not affect the DNA adsorption efficiency of the magnetic beads and subsequent PCR amplification, the phosphoramidate derivative preferentially acts with a phosphate group fixed site on the surface of the soil, adsorption of the soil to the DNA is greatly reduced, an incubation product can be obtained to the maximum extent, then soil impurities are removed through centrifugation, and it is ensured that no adverse effect is generated on subsequent DNA adsorption of the magnetic beads. According to the method, trace DNA can be effectively extracted and purified from old bone detection materials, independent decalcification pretreatment is not needed, sample loss is avoided, and the extracted DNA can be used for forensic medicine identification.

The present invention proposes a polyionic liquid amphiphilic block copolymer brush and a preparation method thereof and a polyionic liquid photonic crystal porous microsphere containing the same, which belongs to the field of photonic crystal microsphere preparation. Specifically, the norbornene-based ionic liquid hydrophilic block is copolymerized with the hydrophobic block through ring-opening metathesis polymerization to obtain a series of amphiphilic block copolymer brushes with different block ratios and different molecular weights; then the brushes are emulsified together with a surfactant aqueous solution, and the organic solvent is volatilized to obtain polyionic liquid photonic crystal porous microspheres. That is, the present invention introduces charged ionic liquids into porous photonic microspheres by polymerizing ionic liquid hydrophilic blocks, which can be used in various fields such as DNA adsorption, DNA encoding, and sewage purification.

The present invention relates to a DNA extraction centrifugal disk. The DNA extraction centrifugal disk comprises at least one DNA extraction unit; the front of the DNA extraction unit is provided with a first liquid inlet, a second liquid inlet, a third liquid inlet, a temporary liquid storage area and a filtration area, and the back is provided with a first liquid storage area and a second liquid storage area; a DNA adsorption material is provided in the filtration area; the first liquid inlet and the second liquid inlet are respectively connected to the filtration area through a flow channel; the third liquid inlet is connected to the temporary liquid storage area through a flow channel, and the temporary liquid storage area is connected to the filtration area through a flow channel; the second liquid storage area is connected to the first liquid storage area through a flow channel, and the first liquid storage area is connected to the filtration area through a flow channel. The DNA adsorption material is preferably a silica gel membrane. The present invention integrates the reaction process of the silica gel membrane adsorption method into the centrifugal disk, which can efficiently realize DNA extraction.

This invention discloses a single-sample multi-omics component extraction method for multi-omics joint analysis. The method includes: adding nucleic acid extraction solution to the sample, transferring it to a DNA adsorption column, centrifuging, and collecting the filtrate; washing the DNA adsorption column; eluting DNA; adding ethanol to the filtrate, transferring it to an RNA adsorption column, centrifuging, and collecting the filtrate; washing the RNA adsorption column; eluting RNA; and extracting proteins. This method integrates centrifugation column-based DNA / RNA co-extraction technology, a zinc ion-mediated protein precipitation and capture strategy, and an efficient Fe-IMAC phosphorylated peptide enrichment process. It achieves efficient protein precipitation and in-situ washing, thoroughly removing chemical components that interfere with mass spectrometry detection. It simultaneously achieves high-quality nucleic acid extraction and deep proteomics and phosphorylated proteomics coverage in a single sample, effectively solving the bottlenecks of traditional methods such as limited sample size, complex procedures, and poor reproducibility.

The present invention relates to the technical field of biological sample extraction, and discloses a method for extracting plasma free DNA. 3+ There are uncoupled electrons in the 4f orbit, which present paramagnetism. The saturation magnetization intensity of the modified magnetic nano-microspheres is relatively high, and the effect of rapid separation can be achieved under the action of a very small magnetic field. By introducing Schiff base and quaternary ammonium salt structure on the surface of chitosan, the winding, coating and connection of magnetic nano-particles are achieved, and the Schiff base is combined with DNA molecules through π-π stacking, thereby achieving the control of DNA adsorption; the cationic quaternary ammonium salt chitosan can effectively combine with DNA with anions, and fix the DNA on the surface of the magnetic microspheres through electrostatic action, thereby achieving the purpose of separation and purification; the free DNA extraction method provided by the invention can prevent the free radicals from destroying the free DNA structure, ensure the structural integrity of the free DNA, and has a simple process and is suitable for large-scale promotion and application.

The invention provides a DNA extraction method and application thereof in multi-chromosome copy number variation analysis, and relates to the technical field of biomedical detection. The DNA extraction method provided by the invention comprises the steps of sample pretreatment, splitting decomposition, DNA adsorption and the like, and the DNA degradation rate of the FFPE sample is controlled below 15%. The detection system based on the extraction method comprises a wet experiment detection module, a biological information analysis module and a clinical decision module, wherein the wet experiment detection module executes the DNA extraction process; the biological information analysis module performs CNV detection through a dynamic sliding window algorithm, and calculates a Z value of a chromosome arm after GC correction and baseline normalization processing; the clinical decision module integrates MaxZ (maximum Z value) and CountZ (abnormal chromosome count) indexes and clinical variables to construct a model, and generates a risk layering report. The method and the system are suitable for multi-chromosome copy number variation analysis, and detection accuracy and clinical application value are improved.

The invention relates to the technical field of biochemistry, in particular to metal-hybridized magnetic nano chitosan and application thereof. Compared with the prior art, the metal-hybridized magnetic chitosan nanoparticles with high DNA adsorption performance provided by the invention have the advantages that the preparation steps are simple and rapid, rapid and high-flux separation of nucleic acid can be realized, and the nanoparticles can be repeatedly used and have wide application value.

The present invention relates to a DNA extraction device. The DNA extraction device comprises a centrifuge and a DNA extraction centrifugal disk, the DNA extraction centrifugal disk is installed on the centrifuge, and the DNA extraction centrifugal disk comprises at least one DNA extraction unit; the front of the DNA extraction unit is provided with a first liquid inlet, a second liquid inlet, a third liquid inlet, a temporary liquid storage area and a filtering area, and the back is provided with a first liquid storage area and a second liquid storage area; a DNA adsorption material is provided in the filtering area; the first liquid inlet and the second liquid inlet are respectively connected to the filtering area through a flow channel; the third liquid inlet is connected to the temporary liquid storage area through a flow channel, and the temporary liquid storage area is connected to the filtering area through a flow channel; the second liquid storage area is connected to the first liquid storage area through a flow channel, and the first liquid storage area is connected to the filtering area through a flow channel. The DNA adsorption material is preferably a silica gel membrane. The present invention integrates the reaction process of the silica gel membrane adsorption method into the centrifugal disk, and can efficiently realize DNA extraction.

The invention discloses an ultra-fast non-high-temperature DNA methylation conversion kit and a use method thereof. The kit is composed of a conversion solution, a protection solution, a binding solution, a rinsing solution, a desulfonation solution, an eluent and a DNA adsorption column. The conversion liquid is prepared from ammonium hydrogen sulfite, sodium hydrogen sulfite and water; the protection liquid is prepared from hydroquinone and water; the binding liquid is prepared from guanidine hydrochloride, sodium chloride and water; the rinsing liquid is prepared from Tris, ethanol and water; the desulfonation liquid is prepared from sodium hydroxide, ethanol and water; the eluent is prepared from a Tris-HCl buffer solution, EDTA (Ethylene Diamine Tetraacetic Acid) and water; and the DNA adsorption column is made of a silicon matrix material. According to the method, the direct transformation time of DNA methylation can be remarkably shortened, meanwhile, the destructive effect of high temperature on a DNA sample is avoided in a non-high-temperature transformation mode, and high recovery rate and high transformation efficiency are guaranteed.

The invention discloses a preparation method and application of TiO2-based bionic flower-like core-shell microspheres for forensic examined material DNA extraction, and belongs to the technical field of biology, the method comprises the following steps: step S1, preparing flower-like functional microspheres; step S2, realizing the coating of SiO2 through a sol-gel method; step S3: preprocessing; s4, carrying out DNA adsorption combination; step S5, washing the DNA precipitate; and step S6, DNA purification and enrichment. The TiO2-based bionic flower-like core-shell microspheres prepared by the method are good in stability and high in DNA enrichment rate, and the morphology and structural characteristics of the microspheres can be influenced by adjusting the quality of Fe3O4 in the microspheres. In addition, the microspheres prepared by the method can be well applied to DNA extraction and other products, and are simple to operate, low in cost and suitable for large-scale production.