3 results about "Dna adsorption" patented technology

This invention discloses a single-sample multi-omics component extraction method for multi-omics joint analysis. The method includes: adding nucleic acid extraction solution to the sample, transferring it to a DNA adsorption column, centrifuging, and collecting the filtrate; washing the DNA adsorption column; eluting DNA; adding ethanol to the filtrate, transferring it to an RNA adsorption column, centrifuging, and collecting the filtrate; washing the RNA adsorption column; eluting RNA; and extracting proteins. This method integrates centrifugation column-based DNA / RNA co-extraction technology, a zinc ion-mediated protein precipitation and capture strategy, and an efficient Fe-IMAC phosphorylated peptide enrichment process. It achieves efficient protein precipitation and in-situ washing, thoroughly removing chemical components that interfere with mass spectrometry detection. It simultaneously achieves high-quality nucleic acid extraction and deep proteomics and phosphorylated proteomics coverage in a single sample, effectively solving the bottlenecks of traditional methods such as limited sample size, complex procedures, and poor reproducibility.

The invention provides a DNA extraction method and application thereof in multi-chromosome copy number variation analysis, and relates to the technical field of biomedical detection. The DNA extraction method provided by the invention comprises the steps of sample pretreatment, splitting decomposition, DNA adsorption and the like, and the DNA degradation rate of the FFPE sample is controlled below 15%. The detection system based on the extraction method comprises a wet experiment detection module, a biological information analysis module and a clinical decision module, wherein the wet experiment detection module executes the DNA extraction process; the biological information analysis module performs CNV detection through a dynamic sliding window algorithm, and calculates a Z value of a chromosome arm after GC correction and baseline normalization processing; the clinical decision module integrates MaxZ (maximum Z value) and CountZ (abnormal chromosome count) indexes and clinical variables to construct a model, and generates a risk layering report. The method and the system are suitable for multi-chromosome copy number variation analysis, and detection accuracy and clinical application value are improved.

The invention discloses a preparation method and application of TiO2-based bionic flower-like core-shell microspheres for forensic examined material DNA extraction, and belongs to the technical field of biology, the method comprises the following steps: step S1, preparing flower-like functional microspheres; step S2, realizing the coating of SiO2 through a sol-gel method; step S3: preprocessing; s4, carrying out DNA adsorption combination; step S5, washing the DNA precipitate; and step S6, DNA purification and enrichment. The TiO2-based bionic flower-like core-shell microspheres prepared by the method are good in stability and high in DNA enrichment rate, and the morphology and structural characteristics of the microspheres can be influenced by adjusting the quality of Fe3O4 in the microspheres. In addition, the microspheres prepared by the method can be well applied to DNA extraction and other products, and are simple to operate, low in cost and suitable for large-scale production.