31results about How to "Reduce physical health damage" patented technology

This invention provides an aquatic dung bacteria DNA distilling kit and its method. In this invention, the dung is disposed by ethanol and TritonX-100 to increase sensitivity of the bacteria to cracking liquid. Then cell is cracked by cracking liquid with SDS, the liquid also has PVP that with absorption to PCR restrictor. Then a high concentration GuSCN, low pH environment is provided for further denaturation of protein in the solution, and the water solubility is reinforced, and the DNA can be binding on silica gel column. Finally, DNA is eluted from the column in low concentration buffer solution or water. The goal of the maximum diversities information, high quality and higher output DNA of aquatic dung bacteria community can be reached by using this kit and its distilling method. The DNA got can be used to molecular ecology, system evolution and other researches.

The invention discloses a soil DNA indirect extraction method for evaluating the diversity of a plant root system microflora. Because the mean density of bacteria is far smaller than the mean density of soil minerals, a sample is pretreated before cell lysis, and microbial cells are separated from the soil sample, thereby avoiding the problems of difficult humus removal and low DNA recovery rate occurring in the purification process. In the extraction process, the invention does not use phenols or chloroform, the damage to the health of an experimenter is reduced, the obtained DNA is complete, a molecular segment is larger than 10kb, and the yield is high. The OD260 / OD230 and the OD260 / OD280 of the extracted soil microbe DNA approach to a standard value, and the soil DNA indirect extraction method can be directly applied to molecular operation so as to evaluate the diversity of the plant root system microflora.

The invention relates to a pot polishing machine. The pot polishing machine comprises a closed machine frame shell. A clamping device and a polishing device are arranged in the machine frame shell, wherein the clamping device is used for clamping a pot body to be polished and driving the pot body to be polished to rotate, and the polishing device is used for polishing the pot body to be polished. Besides, the clamping device and the polishing device are controlled through a digital control device and a hydraulic device. The polishing device is arranged on a shifting mechanism and moved on the shifting mechanism so as to polish the pot body to be polished. The shifting mechanism is composed of two transverse guide rails, two longitudinal guide rails and an oblique guide rail. The transverse guide rails are arranged horizontally. The longitudinal guide rails slide on the transverse guide rails through sliding blocks to constitute a guide rail set shaped like a Chinese character 'jing'. The oblique guide rail is arranged on the longitudinal guide rails in a sliding mode through sliding blocks and fixedly connected with the polishing device through a fixed plate I fixed to the oblique guide rail. The pot polishing machine is provided with the polishing device with the guide rails, a dust removal device, a cooling device and the like, thereby being accurate in precision control, simple in structure, integrally formed, high in working efficiency, capable of improving the environment, safe and durable.

The invention discloses a bacteria genome DNA extracting solution in a blood and platelet product and a preparation method and an application method thereof. The bacteria genome DNA extracting solution consists of chelex-100, tween, NP-40, SDS, lysostaphin, a wall-breaking enzyme, a protease K and water. The extracting solution can be effectively used for extracting a bacteria genome DNA with high extraction efficiency. The preparation method and the application method are simple and convenient for operation.

The invention provides a mounting medium capable of reducing fluorescence decay. The mounting medium capable of reducing the fluorescence decay is characterized by comprising the following components: polyvinyl alcohol, glycerol, diazonium bicyclooctane, a metal ion chelating agent, a carbonate buffering solution, a Tris-HCl buffering solution, a preservative and water; and the mounting medium can be used for mounting a sample capable of emitting fluorescence light or a sample which is dyed by the fluorescence light so as to delay the decay degree of the fluorescence light of the sample, and can also be used for mounting after conventional dyeing. The invention further provides a preparation method of the mounting medium capable of reducing the fluorescence decay, and a mounting method; degrees of various types of fluorescence decay or quenching are greatly reduced; and compared with a common anti-fluorescence-quenching mounting medium, the anti-quenching effect is greatly enhanced.

The invention is applicable to the technical field of building waterproof paint, and discloses environment-friendly fast-setting building waterproof paint which comprises a component A and a component B. The component A is prepared from the following raw materials in parts by weight, 70-80 parts of EVA (ethylene-vinyl acetate) emulsion, 10-15 parts of inorganic fillers, 0.3-0.5 part of coagulating agents, 0.5-1 part of stabilizing agents, 0.2-0.3 part of dispersing agents, 0.2-0.3 part of defoaming agents, 0.15-0.25 part of sterilizing agents and 5-15 parts of water. The component B is prepared from the following raw materials in parts by weight, 12-23 parts of setting accelerators, 2.5-8 parts of promoters and 8-14 parts of water. According to the environment-friendly fast-setting building waterproof paint, the EVA emulsion serves as a main film-forming matter which is the component A, liquid alkali-free setting accelerators and nanometer film-forming promoters form the component B of curing agents, construction can be achieved in an atomizing spray mode, the paint is short in film-forming condensation time, uniform in thickness, high in tensile strength and elongation rate, green and environmentally friendly, construction intensity is greatly reduced, and construction efficiency is improved.

The invention relates to an agent box to distill microorganism gene group DNA from earth and the distilling method. The agent box includes agent A(immersion fluid), agent B(pretreatment fluid), agent C(cracking fluid), and agent D that is made up of RNA enzyme A, agent E Al2(SO4)3, agent F hexadecyl trimethyl amine, and agent G(DNA cleaning fluid). The distilling method includes the following steps: dipping the sample, taking pretreatment to the sample, cracking the microorganism, removing humus, taking DNA coupling, removing protein impurity, adhering DNA, washing DNA, and taking elution to DNA. The advantages of the invention are rapid, little DNA loss, little harmful to researchers, high yield, and low cost, etc.

The invention discloses a soil grinding system. Two extrusion plates are symmetrically installed on the two sides in a grinding tank through spring assemblies. A motor is installed in a movable cover. The rotating shaft end of the motor is connected with a grinding assembly through a fourth electric telescopic rod. The grinding assembly comprises a transverse plate. The transverse plate is connected with a first grinding rod through a first electric telescopic rod set. First grinding rods are connected with second grinding rods through a second electric telescopic rod set. The second grinding rods are connected with third grinding rods through a third electric telescopic rod set. The lower bottom faces of third grinding rods are fixedly connected with a grinding plate. Movable baffles are installed at the two ends of the transverse plate through rotating shafts with power devices. The first grinding rods, the second grinding rods and the third grinding rods are each provided with grinding particles and brush layers alternately. The soil grinding system is simple in structure, reasonable in design and easy and convenient to operate, manpower and material resources are saved, the processing efficiency is improved, harm caused by dust to health of workers and pollution caused by the dust to the environment are reduced, practicability is high, and the soil grinding system is easy to use and popularize.

The invention discloses a soil DNA extracting method for evaluating diversity of a microbial community of a plant root system. In the method, pretreatment is carried out on a sample before cell splitting, extracellular DNA and humic substances are removed, and the problems of difficult removal of the humic substances and low DNA recovery rate existing in a purification step are solved. In the extraction process of the method, phenol or chloroform is not used so as to reduce harm on the health of experimenters, obtain complete DNA and molecular fragments greater than 10kb and achieve high yield. OD260 / OD230 and OD260 / OD280 of the extracted soil microorganism DNA are close to standard values and can be directly applied to molecular operation so as to evaluate the diversity of the microbial community of the plant root system.

The invention discloses a dust removal system and a dust removal method for an electrical screen cabinet. The dust removal system includes: a dust removal room with a door for putting in and taking out the power supply screen cabinet; a window is arranged on the outer wall of the dust removal room; a rotary table , used to place the electrical screen cabinet, the top surface of the rotary table constitutes a part of the bottom surface of the dust removal room, and the rotary table can rotate to drive the electrical screen cabinet to rotate; dust removal equipment, the dust removal equipment The electrical screen cabinet located in the dust removal room can be dedusted through the window. According to the dust removal system and dust removal method of the present invention, operators can perform dust removal work on electrical screen cabinets outside the sealed dust removal room, reducing dust pollution to the environment and health damage to operators.

The invention provides a mixed solvent used for dissolving nitrated cellulose. The mixed solvent contains 1-50wt% of diethyl succinate, 10-60wt% of acetyl tributyl citrate, 10-80wt% of diethylene glycol butyl ether acetate and 1-40wt% of glycerol triacetate. The invention also provides a water-soluble nitrated cellulose product and a water-soluble nitrated cellulose product prepared by the mixed solvent provided by the invention. The nitrated cellulose product prepared by the mixed solvent is little in smell and low in toxicity, has good volatilization grade, and greatly improves the production environment of leather processing enterprises and alleviates the harm to the body health of workers on the basis of keeping the performances of the nitrated cellulose products.