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A Mounting Medium to Reduce Fluorescence Attenuation

A technology of fluorescence attenuation and mounting agent, which is applied in the direction of fluorescence/phosphorescence, material analysis through optical means, material excitation analysis, etc. It can solve the problems of fluorescence attenuation and inability to observe in time, so as to achieve less harm to health and facilitate microscopic examination Observation and storage, the effect of delaying the degree of attenuation

Active Publication Date: 2021-01-01
BEIJING LEAGENE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to various reasons, the sample cannot be observed in time after staining, so it needs to be stored in a special medium to avoid the decrease of fluorescence

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] "A mounting agent for reducing fluorescence attenuation" of the present invention includes the following components per 100ml: 3g of polyvinyl alcohol with an average molecular weight of 85,000-124,000; 18ml of glycerin; 1,4-diazobicyclo-[2,2,2 ] Octane 3g; EDTA.2Na 0.1g; Carbonate buffer (0.1mol / L, pH=8.3) 30ml; Tris-HCl buffer (0.15mol / L, pH=8.7) 25ml; Sodium azide 0.1g ; The rest is deionized water, a total of 100ml.

[0040] Preparation method of "a mounting medium that reduces fluorescence decay":

[0041] Carbonate buffer solution (0.1mol / L, pH=8.3): Dissolve 7.56g of anhydrous sodium bicarbonate and 2.862g of sodium carbonate decahydrate in 900ml of deionized water, the pH value of which is close to 9.16, adjust the pH with hydrochloric acid When the value reaches 8.3, add deionized water to 1000ml and set aside.

[0042] Tris-HCl buffer (0.15mol / L, pH=8.7): Dissolve 18.17g Tris in 900ml deionized water, fully dissolve, adjust the pH value to 8.7 with hydrochlo...

Embodiment 2

[0047] The "mounting agent for reducing fluorescence attenuation" of the present invention includes the following components per 100ml: 1g of polyvinyl alcohol with an average molecular weight of 170,000-220,000; 22ml of glycerin; 1,4-diazobicyclo-[2,2,2 ] Octane 2.5g; EGTA 0.08g; Carbonate buffer (0.2mol / L, pH=8.6) 20ml; Tris-HCl buffer (0.3mol / L, pH=8.4) 15ml; Chloroform 0.1ml; Distilled water, 100ml in total.

[0048] Preparation method of "a mounting medium that reduces fluorescence decay":

[0049] Carbonate buffer solution (0.2mol / L, pH=8.6): Dissolve 15.12g of anhydrous sodium bicarbonate and 5.724g of sodium carbonate decahydrate in 900ml of distilled water, the pH value is close to 9.1, adjust the pH value with hydrochloric acid To 8.6, add distilled water to 1000ml, set aside.

[0050] Tris-HCl buffer solution (0.3mol / L, pH=8.4): Dissolve 36.34g Tris in 900ml distilled water, fully dissolve, adjust the pH value to 8.4 with hydrochloric acid, add distilled water to ...

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PUM

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Abstract

The invention provides a mounting medium capable of reducing fluorescence decay. The mounting medium capable of reducing the fluorescence decay is characterized by comprising the following components: polyvinyl alcohol, glycerol, diazonium bicyclooctane, a metal ion chelating agent, a carbonate buffering solution, a Tris-HCl buffering solution, a preservative and water; and the mounting medium can be used for mounting a sample capable of emitting fluorescence light or a sample which is dyed by the fluorescence light so as to delay the decay degree of the fluorescence light of the sample, and can also be used for mounting after conventional dyeing. The invention further provides a preparation method of the mounting medium capable of reducing the fluorescence decay, and a mounting method; degrees of various types of fluorescence decay or quenching are greatly reduced; and compared with a common anti-fluorescence-quenching mounting medium, the anti-quenching effect is greatly enhanced.

Description

Technical field: [0001] The invention belongs to the application field of biomedicine, and in particular relates to a mounting agent for slowing down fluorescence attenuation or quenching, and a preparation and sealing method thereof. Background technique: [0002] Sealing is to seal and store tissue sections (also blood smears, cell slides, etc.) between the slide glass and the cover glass, so that they do not come into contact with the air, prevent their oxidation and fading, and facilitate microscopic observation. and save. For tissue sections, after staining, after dehydration and transparency, in order to be easy to observe under a microscope and to preserve for future reference, it is necessary to seal the stained slices with a mounting agent (also known as mounting agent, mounting medium, etc.). Thereby completing the making of a tissue slide specimen. In general, a better mounting agent has the following characteristics: 1. Strong viscosity; 2. Better refractive pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 刘涛王红郭喜孙继红陈恺王国强
Owner BEIJING LEAGENE BIOTECH CO LTD
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