Method for extracting DNA from animal tissue efficiently
An animal tissue, high-efficiency technology, applied in the field of DNA extraction, can solve the problems of DNA extraction, such as influence, loss, complicated operation steps, etc., to achieve the effect of reducing health damage, strong applicability, and simple operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0034] Example 1 DNA Extraction from Pig Muscle Tissue
[0035] (1) Cut 100 mg of pig muscle tissue sample, add 750 μL of buffer A, 37.5 μL of proteinase K solution with a mass concentration of 10 mg / mL, wherein buffer A is composed of 30 mM Tris, 50 mM EDTA, 50 mM NaCl, 1% (mass volume % content) Sodium dodecylsulfonate SDS composition, its pH is 7.5~8.0;
[0036] (2) Place the above mixture in a 55~58°C incubator (water bath or small electronic incubation equipment) for 3 hours to obtain a sample suspension;
[0037] (3) Shake for about 1 minute, add 250 μL of 6 mol L to the sample suspension -1 sodium chloride solution, mix well, and centrifuge at 14,000 rpm for 10 minutes at 4°C;
[0038] (4) Transfer 750 μL of the supernatant to a new centrifuge tube;
[0039] (5) Add 500 μL of isopropanol to the supernatant, mix vigorously (vortex for 1 minute), centrifuge at 14,000 rpm for 20 minutes at 4°C, remove the supernatant, and obtain a precipitate;
[0040] (6) Add 1 mL ...
Embodiment 2
[0046] Example 2 Extraction of Pig Liver Tissue DNA
[0047] (1) Cut 50 mg of pig liver tissue sample, add 750 μL of buffer A and 21 μL of proteinase K solution with a mass concentration of 20 mg / mL to obtain a mixture, in which buffer A is composed of 50 mM Tris, 100 mM EDTA, 100 mM NaCl, 2% Composed of sodium dodecylsulfonate SDS, its pH is 7.5~8.0;
[0048] (2) Place the above mixture in a 55~58°C incubator (water bath or small electronic incubation equipment) for 3 hours to obtain a sample suspension;
[0049] (3) Shake for about 1 minute, add 250 μL of 3mol L to the sample suspension -1 sodium chloride solution, mix well, and centrifuge at 14,000 rpm for 15 minutes at 4°C;
[0050] (4) Transfer 750 μL of the supernatant to a new centrifuge tube;
[0051] (5) Add 500 μL of isopropanol to the supernatant, mix vigorously (vortex for 1 minute), centrifuge at 14,000 rpm for 15 minutes at 4°C, remove the supernatant, and obtain a precipitate;
[0052] (6) Add 1 mL of 75% et...
Embodiment 3
[0058] Example 3 Extraction of pig adipose tissue DNA
[0059] (1) Cut out 100 mg of porcine adipose tissue sample, add 750 μL of buffer A, 30 μL of proteinase K solution with a mass concentration of 15 mg / mL, wherein buffer A consists of 80 mM Tris, 200 mM EDTA, 200 mM NaCl, 3% dodecyl Composed of sodium sulfonate SDS, its pH is 7.5~8.0;
[0060] (2) Place the above mixture in a 55~58°C incubator (water bath or small electronic incubation equipment) for 3 hours to obtain a sample suspension;
[0061] (3) Shake for about 1 minute, add 250 μL of 4mol L to the sample suspension -1 sodium chloride solution, mix well, and centrifuge at 14,000 rpm for 15 minutes at 4°C;
[0062] (4) Transfer 750 μL of the supernatant to a new centrifuge tube;
[0063] (5) Add 500 μL of isopropanol to the supernatant, mix vigorously (vortex for 1 minute), centrifuge at 13,000 rpm for 20 minutes at 4°C, remove the supernatant, and obtain a precipitate;
[0064] (6) Add 1 mL of 75% ethanol aqueo...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com