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DNA specimen normal temperature preservation preparation method and packaging equipment

A technology for storing specimens at room temperature, which is applied in the field of DNA preservation, and can solve problems such as low quality preservation, DNA damage, and inability to guarantee

Inactive Publication Date: 2017-05-17
浙江丽生生物科技有限公司
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AI Technical Summary

Problems solved by technology

Each sugar molecule is linked to one of the four bases. These bases are arranged along the long DNA chain. DNA specimens are preserved in the existing technology. In order to prolong the preservation time, high pressure or High temperature treatment to reduce oxygen concentration and sealing treatment will damage the DNA, the quality of preservation is not high, and when sealing, it cannot guarantee an ideal low oxygen environment for operation, and it is difficult to reduce the oxygen in the storage bottle Content, currently there is no method of preservation and packaging at room temperature in the market

Method used

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  • DNA specimen normal temperature preservation preparation method and packaging equipment
  • DNA specimen normal temperature preservation preparation method and packaging equipment
  • DNA specimen normal temperature preservation preparation method and packaging equipment

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Embodiment Construction

[0019] A method for preparing DNA specimens stored at room temperature in the present invention, first of all, to prepare specimens, cut the cotton swabs of oral swabs from the rod with scissors and place them in a 2ml centrifuge tube, add 400 μl Buffer ATL; add 20 μl Proteinase K, and vortex 15 seconds, mix well, 56°C water bath for 30 minutes, mix once during the period; add 400μl Buffer AL, mix well, 70°C water bath for 10 minutes, at this time the solution should become clear, centrifuge briefly to collect the solution on the tube wall At the bottom of the tube; add 400 μl of absolute ethanol, vortex to mix thoroughly, and centrifuge briefly to collect the solution on the tube wall to the bottom of the tube; transfer the solution or emulsion obtained in the previous step to the adsorption column twice, once without For more than 700μl, centrifuge at 8000×g for 1 minute, discard the filtrate, and put the adsorption column back into the collection tube; add 500ul cleaning sol...

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Abstract

The invention relates to a DNA preservation method and equipment, in particular to a DNA specimen normal temperature preservation preparation method and packaging equipment. The DNA specimen normal temperature preservation preparation method comprises the following steps: enabling DNA to be adsorbed on an adsorption film, taking out the adsorption film, placing the adsorption film in a 3ML disinfection clean specimen bottle, then placing the specimen bottle on a DNA normal temperature preservation automatic packaging machine, and placing the DNA normal temperature preservation automatic packaging machine in a vacuum drying box chamber; carrying out first vacuumizing on the vacuum drying box chamber to 0.005MPa, raising the temperature to 10-35 DEG C, and drying for 30mins; filling nitrogen into the vacuum drying box chamber until the pressure is 0.1MPa; carrying out second vacuumizing to 0.005MPa; filling the nitrogen the vacuum drying box chamber secondly until the pressure is 0.1MPa; carrying out third vacuumizing to 0.005MPa; automatically plugging a rubber plug into a bottle mouth of the specimen bottle by the DNA normal temperature preservation automatic packaging machine; and taking out the DNA normal temperature preservation automatic packaging machine and the specimen bottle, adding a metal cover cap at the external of the specimen bottle, and permanently packaging the external of the metal cover cap and the neck of the specimen bottle together with resin. According to the method, harmful gas in the packaging environment is greatly lowered, the DNA is prevented from being damaged by high temperature, and automatic packaging is finished in the vacuum environment.

Description

technical field [0001] The invention relates to a DNA preservation method and equipment, in particular to a preparation method and packaging equipment for DNA specimens stored at room temperature. Background technique [0002] DNA is a long-chain polymer composed of four deoxynucleotides, namely adenine deoxynucleotide (dAMP deoxyadenosine), thymine deoxynucleotide (dTMP deoxythymidine), cytosine deoxynucleotide Nucleic acid (dCMP deoxycytidine), guanine deoxynucleotide (dGMP deoxyguanosine). Deoxyribose (five-carbon sugar) and phosphoric acid molecules are connected by ester bonds to form a long-chain skeleton, which is arranged on the outside, and the four bases are arranged on the inside. Each sugar molecule is linked to one of the four bases. These bases are arranged along the long DNA chain. DNA specimens are preserved in the existing technology. In order to prolong the storage time, high pressure or High temperature treatment to reduce oxygen concentration and sealin...

Claims

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Application Information

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IPC IPC(8): B65D81/18B65D81/20
CPCB65D81/18B65D81/2015B65D81/2076
Inventor 蓝允强黄方亮
Owner 浙江丽生生物科技有限公司
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