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Kit for extracting fungal genome DNA

A genome and kit technology, which is applied in the field of kits for extracting fungal genomic DNA, can solve the problems of long extraction time of DNA samples and achieve the effect of saving extraction time

Inactive Publication Date: 2015-10-14
FUJIAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Develop a convenient, fast and practical method suitable for the extraction of fungal genomic DNA, and solve the problem of long extraction time of DNA samples obtained by conventional methods

Method used

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  • Kit for extracting fungal genome DNA

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Embodiment Construction

[0025] figure 1 Among them, lanes 1, 2, and 3 are the genomic DNAs of yeast, Fusarium, and Penicillium extracted by this kit, respectively, and lane 4 is DL4500Marker from TAKARA Company.

[0026] A kit for extracting fungal genomic DNA, the specific steps are as follows:

[0027] (1) Destruction of fungal cells: Pick 0.5g samples of yeast, Fusarium, and Penicillium in a mortar, add liquid nitrogen and grind them into powder, add the powder to a 1.5ml centrifuge tube, add 500ml Buffer A, 30μl Buffer B, 30μl Buffer C, incubate at 55°C for 6h.

[0028] (2) Extraction of free DNA and impurities: add 500 μl Buffer D for extraction, centrifuge at 12000 rpm / min for 10 min, and transfer the supernatant to a clean 1.5 ml centrifuge tube. Add 500μl Buffer E for extraction, centrifuge at 12000rpm / min for 10min, and transfer the supernatant to a clean 1.5ml centrifuge tube.

[0029] (3) Adsorption of DNA by DNA adsorption column and removal of impurities: add twice the volume of preci...

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Abstract

The invention relates to a kit for extracting fungal genome DNA and belongs to the technical field of biology. The kit for extracting the fungal genome DNA comprises the specific steps of crushing fungal cells, dissociating DNA and removing impure proteins, adsorbing DNA by using a DNA adsorbing column, removing impurities and eluting DNA, finally, so as to obtain a liquid which is the fungal genome DNA which is subjected to electrophoresis detection in 1% sepharose gel. The conventional steps for extracting the fungal genome DNA are tedious, consume a long time, and can extract the fungal genome DNA in about 18 hours generally. The method provided by the invention, compared with the conventional fungal genome extraction method, only needs about 7 hours to extract the fungal genome DNA, so that a lot of extraction time is saved, and the fungal genome DNA can be extracted more effectively, quickly and economically. Meanwhile, the kit is suitable for commercial production and has a certain economic value.

Description

technical field [0001] The invention relates to a kit for extracting fungal genome DNA, which belongs to the field of biotechnology. technical background [0002] Fungi are lower eukaryotic organisms with a large and diverse variety. It is estimated that there are about 1.5 million species of fungi in the world, and about 80,000 species have been described. Fungi are widely distributed in nature, exist in soil, water, air, and inside and outside organisms, and have a very close relationship with human production and life. Many fungi play a major role in the carbon and nitrogen cycles in nature, participating in the decomposition of organic carbon-containing compounds such as starch, cellulose, and lignin, and nitrogen-containing compounds such as protein. Some fungi such as mushrooms, straw mushrooms, fungus, ergot, Cordyceps, Poria, etc. can be directly used for food and medicine, or play an important role in the fermentation industry, food processing industry, and antibi...

Claims

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Application Information

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IPC IPC(8): C12N15/10
Inventor 王清水余彦
Owner FUJIAN NORMAL UNIV
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