Kit and method for extracting pectin-rich polysaccharide plant DNA

A kind of polysaccharide and kit technology, applied in the field of genetic engineering, can solve the problems of difficult to obtain high-quality and high-quantity genomic DNA, inability to obtain DNA, and low DNA quantity, so as to improve DNA quality and yield and avoid secondary quality browning high-quality, high-quality
CN108949751AActive Publication Date: 2018-12-07四川省植物工程研究院
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
四川省植物工程研究院
Publication Date
2018-12-07

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Abstract

The invention relates to a kit and a method for extracting pectin-rich polysaccharide plant DNA. The kit comprises a desaccharifying buffer solution, a lysate, leaching liquor, a column buffer solution, a protein-free solution, a rinsing solution, an eluant and a DNA adsorption column. An extraction method comprises the following steps: removing polysaccharide from a plant, extracting DNA, subsequently extracting and removing polysaccharide again by filtering with a column, thereby acquiring the extracted DNA with high quality and no impurity. The kit and the method provided by the invention are suitable for the extraction of plant genome DNA of mass secondary metabolites rich in polysaccharide, polyphenol, and the like. The kit has the characteristics of a low cost, simple, convenient andquick operation, high quality, high efficiency, high applicability, and the like.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a kit and a method for extracting plant DNA rich in pectin-like polysaccharides. Background technique

[0002] In molecular biology experiments, the quality of DNA isolated from cells is crucial to many subsequent molecular biology experiments, for example, the success or failure of restriction enzyme analysis, Southern blot, DNA library construction, etc. Depends on the quality of the DNA, and the extraction method is an important factor affecting the quality of the DNA. At present, in the extraction of plant genomic DNA, the most commonly used methods are the classic CTAB (Cetyl-trimethyl-ammonium bromide, hexadecyltrimethylammonium bromide) method, SDS (Sodium dodecyl sulfate, dodecyl sulfate Sodium) methods, but these methods are generally only applicable to plants with low secondary substances such as polysaccharides and polyphenols. Due to the di...

Claims

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