DNA extracting method needing no tube transferring

An extraction method and nozzle technology, which is applied in the field of DNA extraction without tube transfer, can solve the problems of cross-contamination and DNA loss of different samples, and achieve the effect of avoiding cross-contamination, avoiding DNA loss, and simplifying the operation steps of DNA extraction
CN106434639AInactive Publication Date: 2017-02-22ANHUI SENPENG BIOTECHNOLOGY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ANHUI SENPENG BIOTECHNOLOGY CO LTD
Publication Date
2017-02-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of nucleic acid extraction and purification and particularly relates to a DNA extracting method needing no tube transferring. The DNA extracting method includes the steps of firstly, preparing reagents, to be more specific, preparing lysate, washing liquid and eluent, and preparing a combined tube; secondly, extracting DNA, to be more specific, placing a sample into the inner-layer splitting tube of the combined tube, adding the lysate and protease k, then performing splitting, centrifuging, allowing liquid in the inner-layer splitting tube to be combined by a nucleic acid absorption film when the liquid penetrates the nucleic acid absorption film in a middle-layer DNA absorption tube, and separating to obtain purified DNA; thirdly, performing PCR amplification and electrophoresis. The DNA extracting method has the advantages that the tube transferring is not needed, the DNA extracting steps are simplified, and detecting time is shortened; DNA loss and cross contamination risks possibly caused by the tube transferring are avoided.
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Description

technical field

[0001] The invention belongs to the technical field of nucleic acid extraction and purification, and in particular relates to a DNA extraction method without tube transfer. Background technique

[0002] DNA is the carrier of genetic information. For eukaryotes, nuclear genomic DNA is located in the nucleus. To obtain purified DNA, it is first necessary to crack the cell membrane, nuclear membrane and other structures to release the DNA, and then remove cell debris, proteins, Lipids, sugars and other impurities to purify DNA; if DNA needs to be extracted from swabs, feces, soil and other samples, it is also necessary to consider removing swabs, feces, soil and other impurities; currently, the available DNA extraction methods include Phenol-chloroform extraction method, magnetic bead method, silica gel membrane method, silica bead method, etc.

[0003] The principle of the phenol-chloroform extraction method is to lyse the cells through the lysate, and use an ...

Claims

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