36 results about "Protease K" patented technology

The invention relates to the technical field of nucleic acid extraction, in particular to a nucleic acid extraction method and a nucleic acid extraction kit. The nucleic acid extraction kit can remove protein in a sample and further release / purify nucleic acid. According to the nucleic acid extraction method, the protease K is added in the washing liquid of the extraction reagent, protein which is deeply combined can be treated, nucleic acid is further released, high-temperature dissociation is used in the nucleic acid elution process, the protease K is inactivated, and the influence on subsequent amplification is avoided.

The invention relates to the technical field of molecular diagnosis, in particular to a high-sensitivity peripheral blood free DNA magnetic bead capture system, a methylation transformation method kit and application. The kit is composed of a lysis solution, a protease K diluent, a composite conversion reagent, a washing solution, a scavenger, an eluent and combined magnetic beads. According to the high-sensitivity peripheral blood free DNA magnetic bead capture system and the methylation transformation method kit, the detection limit of early-stage lung cancer ctDNA reaches 0.005% AF, the I-stage cancer ctDNA detection rate is increased to 93% from the industry average 40%, the sample size is reduced to 4 mL from the conventional 10 mL, the batch-to-batch difference of the kit is small, and the kit has very high application value clinically.

The invention relates to the field of cosmetic compositions, in particular to a cosmetic composition containing triple polishing enzyme and a preparation method of the cosmetic composition. The triple polishing enzyme comprises protease, protease K and superoxide dismutase, the weight part ratio of the protease to the protease K to the superoxide dismutase is 1: (0.5-2): (0.5-2), the total mass percent of the triple polishing enzyme in the composition is 0.0002%-0.001%, and the invention further discloses a preparation method of the cosmetic composition. A triple polishing enzyme synergistic system is formed through scientific compounding, the integrated skin care effect under low concentration is achieved, and the defects of a traditional chemical stripping agent and the shortages of a single enzyme preparation are overcome; meanwhile, by optimizing the proportion of formula components, the composition forms a liquid crystal structure, so that long-acting dual moisturizing of internal water locking and external water sealing is realized, triple polishing enzyme can be wrapped and protected, and the problems that the composition is easy to oxidize and inactivate and low in transdermal efficiency are solved.

The invention discloses a method for efficiently extracting genome DNA (Deoxyribose Nucleic Acid) of ophiurocephalus serratus. The method comprises the following steps: mixing a sample with a lysis solution and metal balls, crushing the mixture at-20 to-10 DEG C for 250-350s at 40-60Hz, and placing the crushed sample at-20 to 4 DEG C; cracking the sample at the temperature of 50-60 DEG C for 4-6 minutes; centrifuging at 2-4 DEG C, taking a supernatant, adding an extraction agent, and uniformly mixing; centrifuging at 2-4 DEG C, taking an upper-layer solution, adding an extracting agent, and uniformly mixing; centrifuging at 2-4 DEG C, taking an upper-layer solution, adding isopropanol, uniformly mixing, and standing at-20--15 DEG C for 20-30 minutes; centrifuging at 2-4 DEG C, discarding the supernatant, washing the precipitate with an ethanol solution, and dissolving with an eluent; the lysis solution contains CTAB (Cetyltrimethyl Ammonium Bromide), protease K and beta-mercaptoethanol. According to the invention, the problem of high-mineralization echinodermis sample treatment is solved, and a high-quality nucleic acid template and a new technology are provided for research on ocean invertebrate genetics.

The invention relates to the field of DNA extraction of leather cultural relics, and discloses a method for extracting cattle genomes from vegetable tanned cattle leather cultural relics. The method comprises the following steps: after cleaning the vegetable tanned cow leather cultural relics, dehydrating, freeze-drying and grinding and crushing with liquid nitrogen; metal ions are chelated through an EDTA-Tris detanning reagent to destroy a tannin structure, so that the wrapping on DNA (Deoxyribose Nucleic Acid) is reduced; then, lysate and protease K are used for lysis and digestion, cell membranes are damaged, protein is degraded, and DNA is released; adding absolute ethyl alcohol to precipitate and separate out DNA; and finally, purifying, recovering and purifying the DNA through an adsorption column. The genome DNA extracted by the method is large in fragment, high in purity and stable and reliable in quality.

The application provides a polypeptide with angiotensin converting enzyme inhibitory activity and a preparation method and application thereof, and relates to the technical field of active peptide preparation. The abalone is pulverized, a complex enzyme is added for primary enzymolysis, and then a specific protease mixture containing prolyl endopeptidase, chymotrypsin and protease K is added for two-step enzymolysis, so that the obtained polypeptide solution has high ACE inhibitory activity. The obtained polypeptide is analyzed and screened to obtain polypeptides with amino acid sequences as shown in SEQ ID NO. 1-5. The polypeptide has high ACE inhibitory activity, and the IC 50 0.05mg / mL-0.40mg / mL, and therefore can play a blood pressure lowering effect as an angiotensin converting enzyme inhibitor.

The invention discloses a plasma free DNA extraction reagent, a kit and an extraction method, the extraction reagent comprises protease K, a lysis solution, a binding solution, a washing solution 1, a washing solution 2, a washing solution 3 and an eluent, the extraction reagents contain more common and less-dosage common reagents such as Tris-HCl (pH 8.0), EDTA.2Na. 2H2O, NaCl, glycerol, SDS, isopropanol, ethanol, Tween-20 and the like. The method has the advantages that the problems that the reagent is easy to separate out and needs to be heated and redissolved for use during storage can be solved without traditional high-concentration guanidine salts such as guanidine hydrochloride and guanidinium isothiocyanate, the stability and the operation convenience of the extraction reagent are increased, a better extraction effect is achieved, and the eluent can effectively protect the extracted cfDNA from being degraded.

The invention belongs to the technical field of food detection, and particularly relates to a detection method for determining the form of trace selenium in vegetables in a geological high background area. The method comprises the following steps: cleaning a vegetable sample, freeze-drying, grinding and crushing, adding a specific compound enzyme system (protease XIV, protease K and cellulase) and a Tris-HCl buffer solution, performing water bath ultrasonic treatment, performing constant-temperature oscillation for enzymolysis, performing low-temperature centrifugation on an extracting solution for multiple times, and filtering and purifying the extracting solution by a microporous filter membrane, the method comprises the following steps: detecting the selenium form of a sample by adopting a high performance liquid chromatograph and inductively coupled plasma mass spectrometer serial device, and quantitatively analyzing tetravalent selenium (Se4 +), hexavalent selenium (Se6 +), selenomethionine (SeMet), methylselenocysteine (SeMeCys) and selenocystine (SeCys2) in the sample by adopting a normalization method. The detection method disclosed by the invention is strong in anti-interference capability and high in sensitivity, and original form information can be completely reserved.

The invention discloses a plant RNA extraction method, and belongs to the technical field of RNA extraction. The extraction method of the plant RNA comprises the following steps: mixing plant tissue powder, a CTAB lysate, a sulfydryl reducing agent and protease K, and then cracking at 60-65 DEG C to obtain a lysate; centrifuging the lysate, taking a supernatant, mixing the supernatant with a first solvent, oscillating, and washing the supernatant to obtain a precipitate; the precipitate and nuclease-free water are mixed and dissolved, and then DNase I and Buffer RDD are added for incubation; continuously adding sodium acetate, an RNA extraction phenol reagent and a second solvent, oscillating, carrying out ice bath, and centrifuging to obtain supernate; carrying out isopropanol precipitation on the supernate to obtain plant RNA (Ribonucleic Acid); the first solvent or the second solvent is a mixture of chloroform and isoamyl alcohol. According to the extraction method provided by the invention, the purpose of completely digesting the genome DNA is achieved on the premise of not influencing the quality of extracted RNA.

The invention relates to an immunofluorescence method and kit for detecting zebra fish heart DNA methylation level, and belongs to the technical field of biological detection. The immunofluorescence method provided by the invention comprises the following steps: exposing a zebrafish embryo to a substance to be detected, dissecting a heart sample of the zebrafish embryo and carrying out fixation, membrane permeation, protease K digestion, hydrochloric acid DNA denaturation and neutralization treatment, and then carrying out sealing, incubation of a 5-methylcytosine primary antibody and a fluorescently labeled secondary antibody, cell nucleus staining and fluorescence detection. And finally, analyzing and evaluating the DNA methylation level through fluorescence imaging. According to the method disclosed by the invention, through a combined step of protease K and hydrochloric acid denaturation treatment, the binding efficiency to the antibody is remarkably improved, so that high-sensitivity and low-background detection on the methylation level of the whole DNA in a zebra fish micro heart sample is realized; the detection kit provided by the invention can be widely applied to epigenetics evaluation of toxicity of environmental pollutants.

The application discloses a DNA / RNA reagent kit based on a magnetic bead method and free of enzyme digestion, and belongs to the technical field of nucleic acid extraction and purification. The reagent kit comprises a lysis solution AL, protease K, a specific binding buffer CLB, amino magnetic bead suspension, silicon hydroxyl magnetic bead suspension and other components. The application solves the problems of nucleic acid degradation, incomplete removal of cross contamination and complicated process caused by enzyme digestion in the prior art, is suitable for automatic high-throughput processing, and has a wide application prospect in scientific research and clinical application.

The invention belongs to the technical field of molecular detection, and discloses a nucleic acid hybridization capture immunofluorescence detection kit as well as a use method and application thereof. In the kit provided by the invention, guanidine hydrochloride, guanidine isothiocyanate, triton X-100, NaCl, protease K and lauryl sodium sulfate are compounded according to a specific concentration to obtain the nucleic acid release liquid, so that pathogen nucleic acid in a clinical sample can be fully dissociated, and the nucleic acid release liquid can cooperate with a probe aiming at a specific target pathogen; the solution system can be used for optimizing the recognition and combination process of DNA and RNA, the specific recognition and combination capacity between the probe and specific pathogen nucleotide is improved, and under the condition that a common nucleic acid stabilizer, namely Tween, is not contained, the solution system can further strengthen differential structural epitopes existing in a DNA / RNA complex compared with DNA and RNA, so that the specificity of the DNA / RNA complex is improved, and the specificity of the DNA / RNA complex is improved. The kit can be better recognized and combined by an anti-DNA / RNA complex antibody, and has excellent detection specificity, sensitivity and precision.

The application discloses a marine organism muscle tissue DNA extraction kit and application thereof, and belongs to the technical field of biological detection. The extraction kit comprises a lysis solution, a first washing solution and a second washing solution. The lysis solution is composed of Triton x-100 45 mM, Tris-HCl 10 mM, EDTA-Na 80 mM, NaCl 80 mM and Brij-35 3 mM, and has a pH of 8.3. The first washing solution is composed of 70% ethanol solution containing 200 mM Tris-HCl. The second washing solution is composed of 80% ethanol solution. The marine organisms include oceanic fishes, Antarctic fishes, shellfishes, crabs and shrimps. The tissue is taken, cut, mixed with the lysis solution and protease K, and uniformly mixed in a water bath. The application is based on the phenol extraction method, and various mild reagents are mixed to realize rapid and accurate DNA extraction, so that the purity, quality and integrity of the DNA are ensured.

The invention discloses an automatic body fluid sample pathogen and host genome nucleic acid synchronous extraction method. The method comprises the following steps: S1, mixing a large-volume body fluid sample with a lysis buffer solution Buffer LA containing chaotropic salt and protease K, and carrying out lysis to release nucleic acid; s2, amino-modified magnetic beads are added, nucleic acid is captured through electrostatic interaction, after room-temperature combination, magnetic separation is conducted, supernatant is abandoned, and the amino-modified magnetic beads are reserved; s3, eluting nucleic acid on the amino-modified magnetic beads, and performing magnetic separation to retain eluent; s4, adding a protective agent Buffer GTN, isopropanol and ion exchange magnetic beads into the eluent, uniformly mixing and combining, performing magnetic separation, discarding supernatant, and retaining the ion exchange magnetic beads; s5, washing the ion exchange magnetic beads, drying, and eluting with Buffer EB1 to obtain whole nucleic acid; according to the method, large-volume, high-efficiency and high-purity nucleic acid synchronous extraction is realized through a double-magnetic-bead staged capturing and cracking-protective agent combination technology.

The invention discloses a kit capable of being used for extracting specific short fragment cfDNA in urine and application of the kit. The kit comprises protease K, a lysis solution, a cleaning solution, an eluent and magnetic beads, wherein the magnetic beads comprise silicon hydroxyl magnetic microspheres and magnetic microspheres modified by streptavidin. According to the technical scheme, through matching of composition control of the magnetic beads and optimization of selection of the components, when the obtained kit is used for extracting free DNA in urine, the extraction efficiency of specific short fragments is greatly improved, and the detection accuracy is further improved.

The invention relates to the technical field of biological detection, in particular to a cat polycystic kidney gene mutation detection kit based on CRI SPR-Cas12a. The kit comprises a freeze-dried CRI SPR reaction ball which is composed of a Cas12a enzyme, a targeted PKD1 gene c.10063Cgt, a CRI SPR gene c.10063Cgt, a CRI SPR gene c.10063Cgt and a CRI SPR gene c.10063Cgt. The kit is formed by premixing and freeze-drying gRNA of a T mutation site and a fluorescence labeling-quenching group reporter probe. The normal-temperature lysis solution is prepared from protease K, a TritonX-100 surfactant and sodium chloride; the chromogenic test strip is formed by arranging two detection lines, namely a T1 line and a T2 line, on a nitrocellulose membrane; and a quality control line C. The CRI SPR reaction ball is freeze-dried to integrate Cas12a enzyme, PAM simulates gRNA and double reporter probes, so that the normal-temperature long-acting stability of key components is realized; a normal-temperature lysis solution is fused with thermal stable protease K and a nuclease inhibition technology, sample DNA is directly released in a short time, degradation is blocked, and the step of centrifugal purification is eliminated.

The invention belongs to the technical field of medical detection, and discloses a staining reagent applied to acid-fast staining and a staining method. The dyeing reagent comprises a primary dyeing agent, a decolorizing agent, a counterdyeing agent and an accelerating agent, wherein the accelerating agent is prepared from the following components: lauryl sodium sulfate, triton X-100, hexadecyl trimethyl ammonium bromide, a Tris-HCl buffer solution, ethylenediamine tetraacetic acid, sodium chloride, potassium chloride, protease K and beta-mercaptoethanol. According to the dyeing reagent, the dyeing accelerant is introduced into a traditional acid-fast dyeing system, the component capable of specifically damaging the lipid structure of the cell wall of the Mycobacterium is utilized, the hindering effect of the lipid structure on the dye in the dyeing process is reduced, the binding efficiency of the dye and thalli is remarkably improved, and therefore the positive detection rate of acid-fast dyeing is greatly increased.

The invention belongs to the technical field of molecular biology, and discloses a preparation method of a fluorescence in situ hybridization kit based on a hybridization chain reaction (HCR), the core components of the kit comprise a hybridization probe, an amplification probe, a washing liquid, a confining liquid, protease K, HRP-mouse anti-digoxin, and a TSA developing liquid 520 nm. According to the FISH detection system combined with the HCR signal amplification technology, when a hybridization probe and an amplification probe are simultaneously combined to a position adjacent to a target sequence, a small section of sequence at the top end can trigger a branched HCR reaction to form a macromolecular nucleic acid aggregate. Signal amplification of HCR is achieved through cascade hybridization reaction, detection sensitivity and specificity can be remarkably improved, and meanwhile the problem of background noise caused by a traditional enzymatic method is avoided. The kit disclosed by the invention is wide in sample application range, and can be used for positioning analysis of target RNA of samples such as paraformaldehyde or formalin-fixed animal and plant tissues, paraffin or frozen sections, cells and the like, and high-sensitivity detection of the single cell level of non-coding RNA.

The invention discloses a targeted protein degradation robot based on DNA origami as well as a construction method and application of the targeted protein degradation robot, and relates to the technical field of biological medicines. The targeted protein degradation robot comprises a DNA origami structure and a recognition module, the construction method of the DNA origami structure comprises the following steps: mixing M13 ssDNA with 60 stapled chains, carrying out annealing reaction to obtain DNA six-screw column origami, and carrying out incubation reaction on the DNA six-screw column origami and a P chain coupled with protease K to obtain origami coupled with inner layer protease K; performing incubation reaction on the origami coupled with the inner layer protease K, a T + S + A-ABD compound and an L + A-ABD compound to obtain an origami compound; and carrying out incubation reaction on the origami compound and human serum albumin (HSA) to obtain the DNA origami structure. The robot can achieve non-endogenous-dependence in-situ precise degradation of target protein, and a new strategy is provided for achieving treatment of diseases such as tumors through interference of the protein level.

The application discloses a preparation method and application of PDRN (plant-derived RNA) from rice callus. The preparation method comprises the following steps: inducing and suspending culturing of the rice callus; homogenizing the rice callus suspension cells obtained in step 1; sequentially using cellulase, pectinase and protease K for enzymolysis; nucleic acid purification and precise ultrasonic shearing are performed in sequence, so that PDRN with a molecular weight of 25-500 bp is obtained. Through optimization of the rice callus culture condition and combination of the joint lysis process of "mechanical grinding-composite enzymolysis-protease treatment", efficient and high-purity preparation of the plant source PDRN is realized, the extraction rate can be higher than 0.5 mg / g of fresh weight of the callus, and the purity A260 / A280 is greater than or equal to 1.8. The obtained PDRN has significant effects in terms of anti-wrinkle, soothing, moisturizing, anti-inflammatory and anti-aging.

The application discloses a crab meat DNA extraction kit and an extraction method, and belongs to the field of molecular biology. The kit comprises a lysis solution, and the lysis solution comprises SDS, protease K and RNase A. The kit is simple in components, easy to prepare, low in cost and free of toxicity. The extraction method is simple and easy to operate, and crab meat DNA can be quickly extracted, thereby promoting the high-quality and sustainable development of the aquaculture industry.

The invention relates to a pretreatment reagent and method for a tissue sample and microorganisms difficult to break walls and application. The pretreatment reagent comprises a first enzyme mixed solution and a second enzyme mixed solution, the first enzyme mixed solution contains protease K and collagenase I, and the second enzyme mixed solution contains lywallzyme and beta-glucanase. A specific pretreatment reagent and a treatment method are designed, a tissue sample is efficiently dissociated into a dispersed cell state by utilizing a first enzyme mixed solution, subsequent grinding and second enzyme mixed solution treatment are facilitated, and the second enzyme mixed solution can greatly improve the release effect of nucleic acid in the sample and has a relatively great promotion effect on subsequent nucleic acid extraction; in addition, specific grinding condition parameters are designed, nucleic acid release can be further promoted, the sample detection performance can be improved, and the probability of false negative in detection is reduced.

The invention relates to the technical field of biomedicine, in particular to a trace blood DNA (deoxyribonucleic acid) extraction method which comprises the following steps: S1, mixing a blood sample and lysate according to a ratio of 1: 3, uniformly mixing by vortex, and centrifuging; s2, protease K is added, vortex mixing is carried out, incubation is carried out at 55 DEG C for 15 min, centrifugation is carried out for 30 s, and 500 microliters of a binding solution is added; and S3, completely transferring the liquid into a purification column with a collection tube, and standing for 2 minutes. The extraction method of the trace blood DNA comprises the following steps: denaturating protein through SDS (Sodium Dodecyl Sulfate), and separating nucleic acid; the binding liquid adopts high salt and low PH, so that the long fragment nucleic acid can be more easily bound on the adsorption column, guanidine hydrochloride can enhance the splitting effect, and the nucleic acid in the blood sample can be fully released due to the dual splitting effect; and on the other hand, the key point is that the step of removing red blood cells by red blood cell lysate is avoided, but the whole blood is directly lysed, so that the loss of white blood cells is reduced, the operation steps are simplified, and the method is suitable for extracting fresh and frozen anticoagulant blood samples.

The invention discloses a method for extracting giardia and cryptosporidium nucleic acid and application thereof. The method comprises the following steps: mixing a sample containing giardia and / or cryptosporidium with lysozyme and protease K, and incubating and digesting; adding a lysis enhancement solution and glass beads into the digested sample, carrying out ultrasonic treatment, and centrifuging after ultrasonic treatment to obtain a supernatant; and carrying out automatic nucleic acid extraction and purification on the obtained supernate. According to the method, the sample treatment time is shortened to be within 3 h, the detection efficiency is greatly improved, the detection cost is reduced, the cracking effect is excellent, the sensitivity is high, and the specificity is high.

The invention relates to a method for quantitatively detecting mycoplasma nucleic acid copy number based on a microdroplet dPCR technology and application, and the method comprises the following steps: adding a lysate into a mycoplasma strain suspension to be detected for lysis, adding protease K for incubation to obtain DNA, diluting, and carrying out dPCR detection; the lysate is prepared from SDS (Sodium Dodecyl Sulfate), Tween-20, Triton X100, EDTA (Ethylene Diamine Tetraacetic Acid), Tris-HCl, beta-mercaptoethanol and sodium chloride; the mycoplasma strain is Mycoplasma hyorhinis, and the mycoplasma strain is Mycoplasma hyorhinis. According to the invention, two cracking reagents are used for cracking a strain suspension, and the cracking solution is diluted by at least 100 times with molecular biological water and then is used for dPCR detection, so that the interference of impurities on an amplification system is reduced; meanwhile, a nucleic acid extraction step is omitted, so that the loss in the nucleic acid extraction process is effectively avoided. Due to the fact that a large amount of free nucleic acid exists in supernate, the method adopts a strain suspension direct cracking mode, and detection and analysis of two parts of nucleic acid of sediment and the supernate can be achieved at the same time.

The invention discloses a multi-enzyme composition for promoting degradation of poly-L-lactic acid facial filling microspheres and a preparation method of the multi-enzyme composition, and belongs to the technical field of enzyme compositions. The multienzyme composition is prepared from the following raw materials in parts by mass: 10 to 15 mg of protease K, 30 to 40 mg of bromelain, 0.75 to 1 mg of collagenase, 20 to 30 mg of esterase, 10 to 15 mg of lipase, 1600 to 1800 mg of mannitol and 600 to 800 mg of sodium carboxymethyl cellulose. All the raw materials are mixed in a phosphate buffer system and prepared into the freeze-dried powder injection. The method has the advantages that multiple enzymes perform their own functions, lipase and esterase have a synergistic effect, the degradation efficiency of poly-L-lactic acid is remarkably improved through an enzymatic hydrolysis-acidic autocatalysis two-stage mechanism, and effective and rapid degradation of the poly-L-lactic acid facial filling microspheres is achieved.

The invention discloses a multifunctional biological scaffold as well as a preparation method and application thereof. The method comprises the following steps: performing proteinase K treatment on a natural egg membrane to obtain a pure substrate; performing gas foaming in the wet substrate by hydrolyzing sodium borohydride to release hydrogen so as to construct a three-dimensional porous scaffold; then, a polydopamine coating is formed on the surface of the stent through dopamine oxidation auto-polymerization, and nano-silver particles are loaded through in-situ reduction; and finally, through simulated body fluid mineralization, a bone-like apatite layer is deposited on the surface of the coating. A uniformly interconnected three-dimensional porous structure is created through gas foaming, and a physical platform is provided for subsequent functionalization. The obtained final material integrates a three-dimensional porous structure, rapid physical-chemical synergistic hemostasis, lasting broad-spectrum antibacterial property and strong osteogenic activity promotion, and is especially suitable for repairing infectious bone defects or refractory soft tissue wounds. The preparation technology is simple, raw materials are easy to obtain, and high-value utilization of waste resources is achieved.

The invention relates to the technical field of molecular biology and in-vitro diagnosis, in particular to a kit and method for directly extracting circulating free DNA (cfDNA) from whole blood, the kit comprises a stationary liquid, a binding liquid, protease K, magnetic beads, a washing liquid, a rinsing liquid A, a rinsing liquid B and an eluent, and the stationary liquid comprises 20% methanol, 0.8 M trehalose and the balance purified water. According to the kit and the method for directly extracting the circulating free DNA (cfDNA) from the whole blood, the process is simplified, plasma separation is not needed, and the manual processing time and the manual error are reduced; cfDNA loss is reduced, and degradation in the plasma transfer process is avoided; the device is suitable for being integrated to a full-automatic nucleic acid extraction platform; the method is high in extraction efficiency, suitable for low-abundance mutation detection and high in sensitivity; and consumables and manual operation cost are reduced.

The invention relates to a method and a kit for extracting DNA from glacier bottom sediment, and belongs to the field of molecular biology. In the prior art, the glacier bottom sediment is extremely low in biomass and complex in mineral composition, so that a traditional DNA extraction method cannot meet the metagenome sequencing requirement. The invention provides an efficient extraction method which comprises the following steps: unfreezing sediments, adding lysate with a specific formula, carrying out repeated freezing and thawing, enzymolysis, centrifugation, organic solvent extraction and the like, finally, carrying out auxiliary precipitation on DNA through PEG8000 and glycogen, and dissolving with sterile water to obtain a high-purity DNA sample. According to the method, the DNA extraction efficiency is remarkably improved, and the concentration can reach gt; 0.1 ng / mu L, and the requirement of metagenome sequencing is met. Meanwhile, the invention further provides a matched kit which comprises key reagents such as lysate, lysozyme and a protease K solution, the proportion of all the components is optimized, and it is ensured that the extraction process is efficient and stable. Experiments prove that compared with the prior art, the method has the advantages that the DNA yield is remarkably increased, the impurity content is greatly reduced, and a powerful tool is provided for glacier bottom sediment microorganism research.

The present application relates to the technical field of extraction reagent, in particular to an economic and general high molecular weight DNA extraction reagent and extraction method, which comprises a lysis solution, a precipitant, a binding solution, a washing solution 1, a washing solution 2, an eluent and a protease K. The present application adopts a precipitation method to extract DNA, avoids using high-priced nucleic acid capture carriers (such as magnetic beads and silica gel membranes) and special extraction instruments, and the reagent components are all conventional inexpensive reagents, so that the cost is reduced by more than 30% compared with existing commercial reagents, and the present application is easy to popularize and widely apply in laboratories.