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Total DNA extraction method for Eremochloa ophiuroides (Munro.) Hack.

An extraction method, the technology of the pseudo-threshold grass, which is applied in the field of molecular biology and can solve the problems of not being able to be applied well

Inactive Publication Date: 2013-12-11
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the total DNA extraction method of one plant often cannot be well applied to the extraction of total DNA of another plant

Method used

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  • Total DNA extraction method for Eremochloa ophiuroides (Munro.) Hack.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Select 2 parts of the current year's leaves (in mid-June) and 2 samples stored at -70°C for one month, a total of 4 genotypes, and use the method of the present invention to perform the following extractions:

[0038] A method for extracting the total DNA of Pseudomonas chinensis, characterized in that: the specific steps are as follows:

[0039] (1) Weigh 0.2g of Herba Cauliflower, grind it in a liquid nitrogen grinder, and transfer the finely ground Herba Cauliflower to a 2mL centrifuge tube;

[0040] (2) Add 0.7mL of CTAB extraction buffer and 14μL of β-mercaptoethanol to a 2ml centrifuge tube, cover the centrifuge tube, mix well, warm bath in 65°C water for 30min, during which time mix well by inversion 5-6 times; among them, The composition of CTAB extraction buffer is: 2%CTAB, 10mM Tris-HCl, 8mM EDTA, 1.4M NaCl, 5%PVP, pH 8.0;

[0041] (3) Take out the sample after the warm bath, cool it to room temperature, add 0.7mL of a mixture containing chloroform and isoamy...

Embodiment 2

[0056] Select 2 parts of the current year's leaves (in mid-June) and 2 samples stored at -70°C for one month, a total of 4 genotypes, and use the method of the present invention to perform the following extractions:

[0057] A method for extracting the total DNA of Pseudomonas chinensis, characterized in that: the specific steps are as follows:

[0058] (1) Weigh 0.2g of Herba Cauliflower, grind it in a liquid nitrogen grinder, and transfer the finely ground Herba Cauliflower to a 2mL centrifuge tube;

[0059] (2) Add 0.7mL of CTAB extraction buffer and 14μL of β-mercaptoethanol to a 2ml centrifuge tube, cover the centrifuge tube, mix well, warm bath in 65°C water for 30min, during which time mix well by inversion 5-6 times; among them, The composition of CTAB extraction buffer is: 2%CTAB, 10mM Tris-HCl, 8mM EDTA, 1.4M NaCl, 5%PVP, pH 8.0;

[0060] (3) Take out the sample after the warm bath, cool it to room temperature, add 0.7mL of a mixture containing chloroform and isoamy...

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Abstract

The invention discloses a total DNA extraction method for Eremochloa ophiuroides (Munro.) Hack. and relates to the technology of extracting total DNA from plants in the field of molecular biology. The total DNA extraction method mainly comprises the following steps: adding a cell nucleus extraction buffer solution into Eremochloa ophiuroides (Munro.) Hack. leaves which are ground into fine powder, and achieving DNA adsorption and elution and DNA purification and separation through silicon beads. According to the invention, the silicon beads are used for effectively adsorbing DNA, removing secondary metabolism products, such as polysaccharose, polyphenols, pigment and protein, and extracting to obtain high-quality Eremochloa ophiuroides (Munro.) Hack. total DNA. The total DNA extraction method can extract high-quality Eremochloa ophiuroides (Munro.) Hack. total DNA from Eremochloa ophiuroides (Munro.) Hack. tissues preserved at low temperature and fresh Eremochloa ophiuroides (Munro.) Hack. tissues, and is suitable for extracting high-quality total DNA from Eremochloa ophiuroides (Munro.) Hack.

Description

technical field [0001] The invention belongs to the field of molecular biology in the herbaceous science, and in particular relates to a method for extracting the total DNA of Pseudomonas chinensis. Background technique [0002] Eremochloa ophiuroides (Munro.) Hack., also known as Centipede Grass and Centipede Grass, is a perennial lawn plant with excellent perennial roots in the Poaceae Centipede grass. It is also the only species of Centipede grass that can be used as lawn grass. In addition to a small amount of distribution in Southeast Asia, Falsegrass is widely distributed in the hills, plains and various wetlands in the Yangtze River Basin and south of my country. It is recognized abroad as the best warm-season turfgrass originating in China. So it is called "Chinese lawn grass". The grass is known for its resistance to barrenness, few pests and diseases, and low maintenance requirements, so it is also called "lazy grass" abroad. In addition, Pseudomonas has very good...

Claims

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Application Information

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IPC IPC(8): C12N15/10
Inventor 王鹏良杨利平覃子海吴幼媚陈晓明陈博雯刘海龙蔡玲韦颖文黄金使黄宏喜
Owner GUANGXI FORESTRY RES INST