Batch and fast DNA extraction method and extraction device

Abstract

The invention provides a batch and fast DNA extraction method and extraction device, and relates to the technical field of a biological device. DNA extraction liquid is added into a deep hole plate; an extraction device is pressed for several seconds; filter paper falls into the extraction liquid; after 3 to 5 seconds, DNA is adsorbed onto the filter paper. The device comprises a press plate, a filter paper groove plate, a protection pipe and elastic posts uniformly distributed on the press plate at equal intervals; the quantity and the space of the elastic posts are identical to the quantityand spacing of holes on the deep hole plate; the head parts of the elastic posts are fixed at one side of the press plate; the filter paper is clamped in the lower end longitudinal center shaft position of the clamping part at the other end; the clamping part is inserted into a downward extending protection pipe of the filter paper groove plate; a spring is sleeved on the outer periphery of the head of the elastic post, so that a certain distance is formed between the filter paper groove plate and the press plate; a slide resistance block for preventing the clamping part from sliding out extends in the axis direction from the top end of the protection pipe; anti-slip bulges corresponding to the slide resistance blocks are arranged on the outer periphery of the clamping part. The method andthe device have the beneficial effects that the fast and batch DNA adsorption is realized; the adhesion between adjacent pieces of filter paper can be prevented; the sample crossed pollution is prevented; the operation is simple; the application range is wide.

Description

technical field [0001] The invention provides a method for rapidly extracting DNA in batches and an extraction device, and relates to the technical field of biological devices. Background technique [0002] DNA extraction from biological samples is the basis of all molecular biology experiments. The traditional nucleic acid DNA extraction process requires professional equipment to obtain the crude extract of nucleic acid lysis; complex crude extract processing steps to wash away pollutants and impurities, and finally isolate and purify high-quality DNA. The whole process needs to be carried out in a professional molecular biology laboratory and requires professionally trained technicians to operate. It takes 2-3 hours to extract a small number of samples (<50). If the sample size increases to thousands or even tens of thousands, the cost of manpower, material resources and time will be inestimable. Studies have shown that cellulose-based filter paper can quickly adsorb ...

AI Technical Summary

Problems solved by technology

[0003] The invention provides a method and an extraction device for rapidly extracting DNA in batches, used in conjunction with deep-well plates, and solves the technical problems that DNA extraction is difficult to perform in simple, fast, and batch manual operations, and DNA extraction samples are cross-contaminated

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