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Souvenir containing DNA and production thereof

A manufacturing method and technology for souvenirs, applied in the field of souvenirs containing DNA, can solve the problems of cumbersome experimental steps, invisible DNA, and DNA content, and achieve the effect of high DNA purity, concise experimental steps, and rapid extraction

Inactive Publication Date: 2006-08-16
哈尔滨基太生物芯片开发有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the defects of cumbersome experimental steps, DNA containing protein, low purity, and almost invisible DNA in the prior art, the present invention provides a souvenir containing DNA and a manufacturing method thereof

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0007] Embodiment 1: The souvenir containing DNA in this embodiment is a fully enclosed container, and the inner cavity of the fully enclosed container is equipped with DNA-absorbing glue beads or glass beads.

[0008] In this embodiment, the inner cavity of the fully enclosed container is a transparent body. The fully enclosed container can be a pendant of ornaments such as crystals, headgear, watches, brooches, rings, earrings or necklaces, or newborn souvenirs or ancestral cards.

specific Embodiment approach 2

[0009] Specific embodiment two: This embodiment manufactures souvenirs containing DNA according to the following methods: a, extract DNA from human body according to conventional methods; b, add 0.1 ~ 0.4ml sodium lauryl sulfate and 0.2 ~ 2ml of rubber beads or glass beads, invert and mix for 5-6 minutes, centrifuge at 9000-14000r / min for 30-60 seconds at room temperature, remove the supernatant; add ethanol to mix thoroughly, and centrifuge at 9000-14000r / min for 30 seconds at room temperature ~60 seconds, remove the supernatant, then add ethanol to mix thoroughly, centrifuge at a speed of 9000~14000r / min at room temperature for 1~5 minutes, remove the supernatant; at this time, the DNA is adsorbed on the rubber beads or glass beads, and then put The glue beads or glass beads are suspended in the DNA storage solution, and stored in an environment of 4° C. for later use; c. Put the glue beads or glass beads adsorbed with DNA into a container, seal it, and isolate it from the ai...

specific Embodiment approach 3

[0011] Specific implementation mode three: this implementation mode manufactures souvenirs containing DNA according to the following method:

[0012] 1. Take 0.1-5ml of venous blood from the human body as a blood sample, and reserve it;

[0013] 2. Prepare and use the following reagents:

[0014] a. 4-10% (w / v) dimethyl salt of ethylenediaminetetraacetic acid;

[0015] b. TNM: 10mM tris-hydrochloric acid (pH=7.6), 10mM sodium chloride, 5mM magnesium chloride;

[0016] c. TE: 10mM tris-hydrochloric acid (pH=8.0), 1mM ethylenediaminetetraacetic acid dimethyl salt (pH=8.0);

[0017] d. 20mg / ml proteinase K;

[0018] e, 0.2% (w / v) sodium lauryl sulfate;

[0019] f, 10% (w / v) sodium lauryl sulfate;

[0020] g. 1ml glue beads or glass beads;

[0021] h, 70% (v / v) ethanol.

[0022] 3. Selected equipment: water bath, ice maker, centrifuge;

[0023] 4. Process:

[0024] ① DNA extraction from blood:

[0025] For blood collection, put 0.1-5ml of whole blood into a test tube con...

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PUM

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Abstract

A souvenir containing DNA is composed of a full-sealed container and the resin bead or glass bead in which the DNA is adsorbed. Its preparing process includes such steps as extracting DNA from a human body, adsorbing the DNA by resin bead or glass bead, loading the resin bead or glass bead in said container, sealing and vacuumizing.

Description

technical field [0001] The invention relates to a souvenir containing DNA and a manufacturing process of the DNA souvenir. Background technique [0002] The Human Genome Project is a major project in the history of human science along with the Manhattan Atomic Project and the Apollo Moon Landing Project. It was officially launched by the U.S. government in October 1990. Scientists from six countries including Germany, Japan, Britain, France, and China joined in successively. 16 laboratories and 1,100 biological scientists, computer experts, and technicians participated. The project produced a "complete map" of the human genome in 2003. The human genome is the sum of the genes that carry all the genetic material on the chromosomes of the human cell nucleus. Chromosomes are twisted double helixes of DNA (deoxyribonucleic acid), the material that makes up genes. There are six trillion cells in the human body, each cell has a nucleus, and chromosomes are located in the nucleu...

Claims

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Application Information

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IPC IPC(8): A44C5/00A44C7/00A44C9/00A44C15/00A44C25/00B44C5/00
Inventor 韩俊李景鹏杨光闫作梅陶嫄于小丽王维海
Owner 哈尔滨基太生物芯片开发有限责任公司
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