Kit and method for quickly extracting DNA of plant by use of DNA adsorption column

An adsorption column and kit technology, applied in the field of kits for rapid extraction of plant DNA, can solve the problems of easy degradation, loss, and time-consuming, and achieve the effect of high purity and good integrity

Inactive Publication Date: 2014-12-03
FUJIAN NORMAL UNIV
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AI Technical Summary

Problems solved by technology

The traditional plant tissue DNA extraction process is time-consuming and prone to DNA loss and degradation.
[0004] Therefore, it is necessary to develop a convenient, fast and practical method suitable for plant genomic DNA extraction, so as to solve the shortcomings of DNA samples obtained by conventional methods such as poor integrity and easy degradation.

Method used

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  • Kit and method for quickly extracting DNA of plant by use of DNA adsorption column
  • Kit and method for quickly extracting DNA of plant by use of DNA adsorption column

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Experimental program
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Effect test

Embodiment Construction

[0025] A kit and method for rapidly extracting plant DNA using a DNA adsorption column, the specific steps are as follows:

[0026] (1) Raw materials: Weigh 1 g of each of the five plant samples of rice, sugarcane, corn, peanut, and wheat.

[0027] (2) Pretreatment of plant tissue samples: Grind the weighed rice, sugarcane, corn, peanut, and wheat tissues into powder with liquid nitrogen.

[0028] (3) Extraction of free DNA and impurity proteins: put the sample powder into a 2ml centrifuge tube, add 800μl lysate, shake the oscillator for 30 seconds, incubate in a 65°C water bath for 30 minutes, every 5 minutes Shake once. After incubation, 800 μl of extract was added, shaken for 30 seconds, and then centrifuged at 12000 rpm / min for 10 minutes. Transfer the supernatant to a new centrifuge tube.

[0029] (4) Adsorption of DNA by DNA adsorption column and removal of impurities: add an equal volume of precipitant to the centrifuge tube containing the supernatant, mix well and a...

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Abstract

The invention relates to a kit and a method for quickly extracting a DNA of a plant by use of a DNA adsorption column, and belongs to the technical field of biology. The kit comprises such components as a splitting liquor, a leaching liquor, a precipitator, a deproteinizing liquor, a rinsing liquor, an eluent and the DNA adsorption column. The method comprises pretreatment of a plant tissue sample, dissociation of DNA and removal of impurity proteins, adsorption of DNA by use of the DNA adsorption column and removal of impurities, and DNA elution. The kit can be used for quickly extracting the genome DNA of the plant, and the obtained genome DNA has relatively high purity.

Description

technical field [0001] The invention relates to a kit and a method for quickly extracting plant DNA by using a DNA adsorption column, belonging to the field of biotechnology. Background technique [0002] DNA extraction is the basic technology of plant molecular biology research. The continuous development of DNA extraction technology has created favorable conditions for many applications such as whole genome sequencing. With the development of modern molecular biology, DNA isolation techniques emerge in endlessly. DNA can already be isolated from extreme materials such as fossils, mummies, etc., and can also be obtained from trace materials. At present, a variety of methods have been developed to successfully extract DNA from plant leaves, callus, tissue culture seedlings, fruits, phloem and other tissues and organs. [0003] Traditional DNA extraction and purification, such as CTAB method and SDS method, are based on lysed cells, repeated extractions with organic solven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 王清水余彦
Owner FUJIAN NORMAL UNIV
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