Kit for rapid extraction of plant seed DNA, and application thereof

A plant seed and kit technology, applied in the direction of DNA preparation, recombinant DNA technology, etc., can solve the problems of high impurities and low DNA yield, achieve high purity, improve yield and purity, and high yield
CN103289991AActive Publication Date: 2013-09-11THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Publication Date
2013-09-11

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Abstract

The invention discloses a kit for rapid extraction of plant seed DNA, and an application thereof. The compositions of the kit comprise: a CTAB lysate, a silicon-based plasmalemma DNA adsorption column, an RNA enzyme, a buffer solution for passing through a column, a rinsing solution 1, a rinsing solution 2 and an eluting buffer solution; the compositions of the buffer solution for passing through the column include: 2.5-7.0 M of guanidine hydrochloride, 0.05 M of Tris-HCl, and 0.01 M of EDTA; and the pH value is 4.0-7.0. The invention further discloses the application of the kit for the rapid extraction of the high quality plant seed DNA. The kit can effectively improve yield and purity of the extraction of the plant seed DNA, has the extracted DNA with good integrity, high purity and high yield; while decreasing DNA extraction cost, the kit effectively improves extraction quality and extraction efficiency of the plant seed genomic DNA.
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Description

technical field

[0001] The invention relates to a DNA extraction kit, in particular to a rapid plant seed DNA extraction kit and an application thereof, belonging to the field of plant seed DNA extraction. Background technique

[0002] In molecular biology research, the extraction of high-quality DNA is the key to its downstream biological applications. With the development of molecular biology, DNA extraction methods of various plant materials have been established, including: CTAB method, SDS method, PVP method, urea method or high salt and low pH method, etc. The contents of proteins, polysaccharides, and phenolic substances in different plant materials vary greatly, and it is difficult to separate or purify high-quality DNA. In view of the differences in the cell walls and cell contents of different plant materials, the quality of DNA extraction can be guaranteed only by adopting an appropriate plant DNA extraction method.

[0003] Plant seeds usually contain a large a...

Claims

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