Efficient plasma cell dissociation DNA extraction method based on paramagnetic particle method
An extraction method, the technology of magnetic bead method, applied in the biological field, can solve the problems of low extraction efficiency, easy loss of DNA fragments, uneven batch operation effect, etc., and achieve the effect of promoting adsorption and improving extraction efficiency
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0023] Example 1
[0024] A high-efficiency method for extracting free DNA from plasma cells based on the magnetic bead method is characterized in that the specific steps are as follows:
[0025] (1) Pretreatment
[0026] Set up three parallel experiments. Take 200μl of fresh plasma sample from healthy people and place it in a centrifuge tube, add 20μl of pretreatment solution P, and store it in a 50℃ water bath for 0.5h;
[0027] (2) Adsorption
[0028] Add 800μl binding solution B to the pretreated sample; vortex for 30s; react for 5min in a 50℃ water bath; remove the centrifuge tube from the water bath and vortex for 15s; use a rotary mixer to react for 10min at room temperature; end of timing Then, centrifuge at 4000g for 30 seconds, put the centrifuge tube on the magnetic stand, and let it stand for 2 minutes to separate the magnetic beads. After the timing is over, use a pipette to remove the supernatant and retain the magnetic beads;
[0029] (3) Washing
[0030] The first step i...
Example Embodiment
[0042] Example 2:
[0043] In step (1), the same amount of isopropanol was used to replace n-hexane when preparing the binding solution B. The other steps and dosages were the same as in Example 1. Three sets of substitution comparison tests were performed, and the DNA yield decreased by about 22.3%. The results are shown in Table 2. Show.
[0044] Table 2 Extraction results of plasma cell free DNA
[0045]
Example Embodiment
[0046] Example 3:
[0047] In step (2), the DNA adsorption process was changed to vortex oscillation for 30s; the reaction was performed at room temperature for 15 minutes, and the other steps and dosage were the same as in Example 1. Three sets of alternative comparison experiments were performed, and the DNA yield decreased by about 16.5%. The results are shown in Table 3. Shown.
[0048] Table 3 Extraction results of plasma cell free DNA
[0049]
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap