The invention belongs to the technical field of bioengineering, and relates to a method for promoting sheep in-vitro
embryo development by using
follistatin, which comprises the following steps: (1) acquiring a slaughtered sheep
ovary; (2) extracting follicles for later use; (3) culturing selected oocytes in a CO2
incubator, and adding into in-vitro fertilization
solution drops; (4) adding the in-vitro fertilization drops, and carrying out co-incubation with the oocytes; (5) taking out the early
embryo, transferring into a 10 mu g / L
follistatin in-vitro culture solution, washing 3-4 times, and culturing for 24-26 hours; and (6) taking out the
embryo, washing with the in-vitro culture solution, calculating the merogenesis rate, and calculating the
blastula rate after culturing for 144-168 hours. By using the
follistatin to treat the early embryo subjected to sheep
oocyte in-vitro fertilization, the method can obviously enhance the in-vitro embryo development
blastula rate.