35 results about "Meloidogyne arenaria" patented technology

The subject invention provides novel and advantageous methods for growing bacteria. The methods of the subject invention are particularly advantageous for growing parasitic bacteria, in vitro, without the presence of host tissue. In one embodiment of the subject invention, Pasteuria spores, such as those that infect the rootknot nematode Meloidogyne arenaria or other host nematodes, are grown in vitro. The process of the subject invention is highly advantageous because Pasteuria can be grown in the absence of nematode tissue.

The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.

The invention discloses primers and a method for directly and simultaneously detecting Meloidogyne and Rotylenchulus reniformis in soil and belongs to the technical field of molecular biology detection. Nucleotide sequences of the primers for directly and simultaneously detecting the Meloidogyne and the Rotylenchulus reniformis in the soil are shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 respectively. The invention also discloses the method for directly and simultaneously detecting the Meloidogyne and the Rotylenchulus reniformis in the soil. The method comprises the following steps of: directly extracting deoxyribonucleic acid (DNA) from soil, purifying, performing first-round polymerase chain reaction (PCR) by using the universal primers of nematodes, namely D2A and D3B by taking the purified DNA as a template, performing second-round PCR by using the primers which are shown as SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6 by taking a product as a template, and judging whether the Meloidogyne and the Rotylenchulus reniformis are contained in a sample through the magnitude of fragments of a PCR product. The primers are sensitive and high in specificity; by the method for directly and simultaneously detecting the Meloidogyne and the Rotylenchulus reniformis in the soil, the detection time can be greatly saved, and results are accurate and reliable; and the primers have high practical application value in the aspect of the rapid detection of the Meloidogyne and the Rotylenchulus reniformis.

The invention relates to wettable powder, powder or a microgranule preparation prepared from accessory ingredients, such as a wetting agent, a dispersing agent, fillers and the like, and a pesticide composition containing dazomet and 1,3-dichloropropylene for controlling soil-borne diseases such as Phytoph-thora capsici Leonian and the like as well as Meloidogyne. The invention also discloses a method for preventing the soil-borne diseases such as the Phytoph-thora capsici Leonian and the like as well as Meloidogyne by using the composition containing the dazomet and the 1,3-dichloropropylene. The dazomet and the 1,3-dichloropropylene are mixed at the ratio of (1:2)-(2:1) to especially perform action on the Phytoph-thora capsici Leonian and the Meloidogyne. The pesticide composition is not only used for controlling the Phytoph-thora capsici Leonian and the Meloidogyne, but also widely applied to the aspects of controlling the soil-borne diseases (including the meloidogyne) in vegetables, such as hot peppers, gingers, cucumbers and the like, field crops and flowers. The pesticide composition has high application value and obvious economic and social benefits.

The invention discloses a preparation for prevention and treatment of tobacco and tomato meloidogyne spp. and an application thereof. The preparation for prevention and treatment of tobacco and tomato meloidogyne spp. has an active ingredient of a fermentation liquid of a strain of bacillus amyloliquefaciens YN2010-KC CGMCC No.5722. The preparation not only is a microbial fertilizer which can significantly promote the growth of plants, but also is a biological pesticide and has the average relative control efficiency on tobacco meloidogyne spp. disease of up to 78.32%; the preparation has the control efficiency on the tomato meloidogyne spp. disease of up to 74.50% or more, and has remarkable prevention and treatment effect. The preparation has low production cost, an application method is simple and easy to implement, and the preparation can be used for direct seed dressing and root irrigation, has no toxicity, no pollution and no residue, and is safe to human and livestock.

The subject invention provides novel and advantageous methods for growing bacteria. The methods of the subject invention are particularly advantageous for growing parasitic bacteria in vitro, without the presence of host tissue. In one embodiment of the subject invention, Pasteuria endospores, such as those that infect the rootknot nematode Meloidogyne arenaria or other host nematodes, are grown in vitro under acidic conditions. The process of the subject invention is highly advantageous because Pasteuria can be grown in the absence of nematode tissue.

The present invention discloses one kind of pesticide for preventing and killing soil nematode and other pests and its preparation. Emamectin benzoate as main material is mixed with solvent, emulsifier and penetrant to form the pesticide oil cream. The pesticide oil cream is diluted and irradiated to roots or sprayed to soil so as to prevent and controlling meloidogyne hapla and other pests. It has simple use, low consumption, high pesticidal effect, fast degradation and no residue, and may be used in produce green food material.

The invention relates to the technical field of prevention and treatment of plant diseases, in particular to application of benzoic acid in prevention and treatment of plant nematode diseases, wherein the benzoic acid is used as an effective component in nematicide. Based on the research result of the invention, the benzoic acid has strong toxic activity on various nematodes such as Meloidogyne, Heterodera, Bursaphelenchusxylophilus and the like, can be used for effectively preventing and treating plant nematode diseases, has the advantages of low harm to a human body, low toxicity, low residue, easiness in degradation, greenness, environmental protection and the like, and has good application values are realized in the aspects of preventing and controlling the plant nematodes and developing related medicament products.

The invention provides an isolated Purpureocillium lilacinum strain, a composition containing the same, and an application of the strain in prevention and treatment of Meloidogyne. The strain is highin sporulation capacity, high in viable count and good in prevention and treatment effect, and can be used for prevention and treatment of various plants.

The invention discloses a meloidogyne culture medium. The meloidogyne culture medium comprises compound amino acid powder, fish meal, calcium chloride dihydrate, agar powder and water. By adding the compound amino acid powder and the fish meal into the water agar culture medium, the yield of the meloidogyne can be greatly increased, and the period is shortened, so that the invention provides a simple, convenient and rapid culture method capable of obtaining a large number of plant meloidogyne, and the problems of dependence on hosts, tedious operation, long culture time and small meloidogyne number in the existing meloidogyne culture are solved.

The invention discloses a marigold straw organic fertilizer with a nematode control function and a preparation method thereof. The marigold straw organic fertilizer is prepared from the following rawmaterials in parts by weight: 800-900 parts of marigold straw, 80-150 parts of rape cake, 0.2-0.5 part of a decomposing inoculant, 15-30 parts of ammonium nitrate, 0.2-0.5 part of potassium phosphiteand 4.6-19 parts of potassium silicate, wherein the decomposing inoculant is prepared from trichoderma viride, aspergillus niger and bacillus subtilis in an equal mass ratio; the organic fertilizer provided by the invention can effectively improve the conversion of nematocidal active ingredients through secondary fermentation, and enhance the reduction rate of meloidogyne in soil.

The invention discloses a quasi-trichoderma harzianum strain and an application thereof. The stain is quasi-trichoderma harzianum strain Thar2 which is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC NO.10924. For the application of a biocontrol seed coating agent prepared from the quasi-trichoderma harzianum strain Thar2 in prevention and treatment of Meloidogyne, the quasi-trichoderma harzianum Thar2 strain is a fungus obtained by being separated from soil, is harmless to crops, has prevention and treatment functions for the Meloidogyne, has safety in the application and is suitable for biological control of the Meloidogyne. The death rate of quasi-trichoderma harzianum Thar2 strain fermentation broth on second instar larvae of the Meloidogyne reaches over 98.8 percent, the prevention and treatment efficiency in a greenhouse reaches 83.5 percent, the prevention and treatment efficiency is high and stable, and the quasi-trichoderma harzianum Thar2 strain fermentation broth is suitable for agricultural production requirements.

The invention discloses a method for separating meloidogyne incognita egg granules, which comprises the following steps of: performing static incubation, NaClO dissociation, oscillation, centrifugation, cleaning and the like on meloidogyne incognita egg blocks stripped from the surface of a plant root system, so that pure meloidogyne incognita egg granules can be separated, meanwhile, higher egg activity is ensured, and the method has popularization and application values.

The invention discloses a method for preventing and treating fruit and vegetable meloidogyne disease. The method comprises the following steps: treating fruit and vegetable vegetables by using a meloidogyne disease treating powder and paecilomyces lilacinus; spreading meloidogyne treating powder based on 2-4kg/ mu as base fertilizer to the land during land preparation; performing rotary tillage, bedding, film covering, holing and field planting; dropwise irrigating a paecilomyces lilacinus aqueous solution after seedlings of the fruit and vegetable slowly grow once every 20-30 days based on 1000-2000ml/ mu; and stopping dropwise irrigating 7d before uprooting. With the adoption of the method, the fruit and vegetable meloidogyne disease can be effectively prevented and treated, and the preventing and treating effect reaches 89.07-95.13%.

The invention provides Bacillus aerophilus ZDXC-01. The collection number of Bacillus aerophilus ZDXC-01 is CGMCC (China general microbiological culture collection center) No.14104. The invention further provides a 16S rRNA gene segment of the strain, a fermentation liquid, a fermentation method, an application of the strain in prevention of meloidogyne and the like.

The invention relates to the technical field of microorganisms, and particularly provides ascophyllum nodosum enzymatic hydrolysate, a process for culturing EM bacteria by using the ascophyllum nodosum enzymatic hydrolysate as a culture medium component, and an application of the cultured EM bacteria to prevention and treatment of meloidogyne. The ascophyllum nodosum enzymatic hydrolysate is obtained by performing enzymolysis on ascophyllum nodosum by using ADL compound enzyme, and can be used as an important component to be added into a culture medium for culturing the EM bacteria, so that stable and high-yield viable bacteria can be obtained. Research on obtained fermentation liquid shows that the fermentation liquid has an extremely remarkable prevention and control effect on M.incognita, a new direction is provided for prevention and control of the meloidogyne, and the fermentation liquid better serves agricultural production.

The invention discloses a kit for detecting Meloidogyne arenria and belongs to the field of biotechnical detection. According to the technical scheme, the kit for detecting Meloidogyne arenria comprises a Mg<2+>-free 10*PCR buffer solution, a MgCl2 solution of which the concentration is 25mM, a dNTP solution of which the concentration is 0.1mM and a taq enzyme solution of which the concentration is 5U/mu l as well as an upstream primer solution and a downstream primer solution of which the concentrations are both 10mu M, wherein the upstream primer solution and downstream primer solution comprise a pair of specific primers, the nucleotide sequences of the pair of specific primers respectively are as follows: the upstream primer 5, -GTGAGGATTCAGCTCCCCAG-3, and the downstream primer 5, -CAAGACCCAATGGCCATAGG-3. According to the invention, the technical problems of low sensitivity, poor stability, cumbersome operation and environmental pollution in the existing Meloidogyne arenria detection technology are solved.