38 results about "Meloidogyne arenaria" patented technology

The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.

The invention discloses a method for preparing a transplanting matrix for blueberry pot seedlings. The method comprises the following steps: (1) surveying the soil pH of a seedling raising field; (2) deeply plowing the seedling raising field by the depth of 30-40cm, and removing foreign bodies such as stone; (3) applying sulfur powder to the seedling raising field so as to adjust the soil pH value to an appropriate pH value, meanwhile, broadcasting dazomet to the seedling raising field, carrying out uniform stirring, and then, covering the seedling raising field with films for fumigation; (4) adding peat and fermented sawdust which are in a certain ratio into soil, so as to improve the soil and increase the organic matter content of the soil; and (5) carrying out uniform stirring mechanically, so as to obtain a mixture for later use. According to the method, the dazomet is adopted to fumigate the soil, so that the disease damage caused by meloidogyne spp. is avoided, and the cost for treating weeds is reduced; meanwhile, the soil pH value is adjusted by using the sulfur powder, and a synergetic effect of the dazomet and the sulfur is generated, so as to obtain a better effect; and the fermented sawdust and the peat are added and interact with the sulfur, so that the structural formation of soil aggregates is promoted, the organic matter content is increased, the quality of the soil is improved, and the success rate of blueberry seedling raising is greatly increased.

The invention relates to a fungi culture method, which has toxic action on plant parasitic nematode. The invention also relates to a metabolite preparation method of the fungi and the application, which belongs to the technology area of microbial pesticide. The Trichoderma spp strain Snef85 related to the invention is Trichoderma harzianum, which is stored in General Microbiological Culture Collection Center (address: Datun Road, Chaoyang District, Beijing, China, 100101) on 30 October, 2007. And the preservation number of the strain is CGMCC No: M2235. The taxonomic status of Trichoderma sppstrain Snef85 is Deuteromycotina, Hyphomycetales, Moniliales, Moniliaceae, Trichoderma, Harzianum. The fungi is prepared through liquid fermentation culture method; the metabolite has the outstandingcharacteristics of strong toxic action and significant nematode killing effect on plant parasitic nematode, such as Meloidogyne spp., Aphelenchoides besseyi and Heterodera glycines especially. The fermentation broth of the strain has excellent control effect on Meloidogyne spp., Aphelenchoides besseyi and Heterodera glycines. Significant differences were observed between treatment with sterilizedwater and with Aspergillus fermentation broth at different concentrations. The nematode killing effect of fermentation broth can reach more than 95 percent. The invention has great application and development prospect.

The invention discloses a method for detecting a single knot of enterolobium cyclocarpum meloidogyne and application thereof. In the invention, universal primers MF (Forward Primer) and MR (Reverse Primer) for amplifying meloidogyne and specific primers FMe (Forward Primer) and RMe (Reverse Primer) for amplifying the enterolobium cyclocarpum meloidogyne are designed; a single root knot DNA containing the meloidogyne is taken as a template for PCR (Polymerase Chain Reaction) amplification reaction; a PCR amplification reaction product is subjected to lipolysaccharide electrophoresis detection, and a detection result is obtained according to the existence / absence and the size of straps; and the sequences of the primers are respectively shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4 in a table. The one-step dual PCR detection method disclosed by the invention has the advantages of increased molecular detection speed, increased sensitivity and accuracy, and has a practical application value in the aspect of detecting the enterolobium cyclocarpum meloidogyne.